Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000564-TA|BGIBMGA000564-PA|IPR001382|Glycoside
hydrolase, family 47
         (533 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide 1,2-al...   331   2e-91
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar...   146   5e-36
SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity prote...    29   1.6  
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...    27   4.9  
SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe...    27   4.9  
SPBC1347.09 |||hexaprenyldihydroxybenzoate methyltransferase|Sch...    27   6.5  
SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr ...    27   6.5  
SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2...    27   6.5  
SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po...    27   8.6  

>SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide
           1,2-alpha-mannosidase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 521

 Score =  331 bits (813), Expect = 2e-91
 Identities = 188/486 (38%), Positives = 285/486 (58%), Gaps = 26/486 (5%)

Query: 73  TVYERPVFTGPKND-RQRALVE-SFRHAWRGYKEHAWGHDNLKPVSGMASDWF--SLGLT 128
           TV   P +T  K   R++AL++ +F  +W  Y+ + WG D   P+     ++    +G  
Sbjct: 30  TVQRPPGYTKLKGSTRRKALIKKTFIESWTDYETYGWGKDEYYPIIKRGRNYLRKGMGWM 89

Query: 129 IVDSLDTAYIMGLTDVFEEGKQWIKEELIFTKN-KDVNFFEVTIRILGGLLTNHYFTQDQ 187
           I+DSLDT  IMGL +     ++W+   L + ++ ++V+ FE TIRILGGLL++++ +QD+
Sbjct: 90  IIDSLDTMMIMGLDEQVLRAREWVNNSLTWNQDDEEVSVFETTIRILGGLLSSYHLSQDK 149

Query: 188 LFLDKAIDLGDRLMAAFSSPSGIPYSDVNLGARTAHAPEWSHYSTTAEVTTVQLEFRQLS 247
           L+LD+A+DL DRL+AA+++ +G+P S+VNLG R +      ++ +TAE  TVQ+E R LS
Sbjct: 150 LYLDRAVDLADRLLAAYNTSTGLPRSNVNLGTRKSRKRTREYFVSTAESGTVQMELRYLS 209

Query: 248 RASGNPIFEEAAAAVSE-KIHQLPKKH-GLVPIFINPNTGHFLPHATITLGARGDSYYEY 305
             +G+P +   A    E  +      H GLVPI +N  TG ++    I LG+ GDSYYEY
Sbjct: 210 YLTGDPKYWITADKTMEVLLGDATWSHTGLVPITVNLITGAYVGR-NIRLGSHGDSYYEY 268

Query: 306 LLKQWLQTGKTINYLLDDYLTGIEGVREFLAKRSSPNKRLFIGELSAGSE-AFNPKMDHL 364
           LLKQ LQ   +       +   ++G+ E+L   ++PN   +I EL  G E A  PKMDHL
Sbjct: 269 LLKQDLQLFSSGTVYRKAFDLSVDGIIEYLLNYTTPNHFAYIAELPGGLEHAQLPKMDHL 328

Query: 365 TCFLPGTLALGHLNGL--------PDW------HMTMAEELLYTCYLTYAAHPTFLAPEI 410
            CFLPGTL  G  NG          +W       + +A+EL+ TCY  Y    T LAPEI
Sbjct: 329 VCFLPGTLMWGATNGTSLEAARTSKNWGTRQERDVKLAQELMRTCYEMYNMTATGLAPEI 388

Query: 411 THFNLASTTDDMYTKSADAHSLLRPEFIESLWYMYQITGNTTYQEWGWQIFQGFEKYAKV 470
             F++  T +++Y+K  D H+L+RPE +ESL+ +Y+IT +  Y+EWGW IF  F +Y+++
Sbjct: 389 VFFDVDQTKNEIYSKRRDQHNLMRPETVESLFILYRITRDEIYREWGWNIFVSFLRYSRL 448

Query: 471 P--NGYSSLTNVKSEN-PIPRDMMESFFLSETXXXXXXXXSDDRFLIDLNKYVVNSEAHP 527
           P  + ++ L +V+S+     RD  ESF+ +ET         DD  ++ L  Y  N+EAHP
Sbjct: 449 PGRDAFTCLDSVESKKVKDQRDKTESFWFAETLKYLYLLFEDDFSILPLTNYTFNTEAHP 508

Query: 528 LPIHKN 533
            P  +N
Sbjct: 509 FPNIEN 514


>SPAC23A1.04c |mnl1||alpha mannosidase-like
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score =  146 bits (355), Expect = 5e-36
 Identities = 129/464 (27%), Positives = 219/464 (47%), Gaps = 57/464 (12%)

Query: 95  FRHAWRGYKEHAWGHDNLKPVS--GMASDWFS---LG---------LTIVDSLDTAYIMG 140
           F H +  Y + A+ +D L P+S  G+  D+ +   +G         LT+VD LDT  ++G
Sbjct: 43  FYHGYNNYMQFAFPNDELAPLSCEGLGPDYENPNNIGVNDVRGDYLLTLVDVLDTLVVLG 102

Query: 141 LTDVFEEGKQWIKEELIFTKNKDVNFFEVTIRILGGLLTNHYFTQDQLF----------- 189
             + F++    +   + F ++  V  FE TIRILGGLL++H F  ++ +           
Sbjct: 103 DREGFQDAVDKVIHHINFERDTKVQVFEATIRILGGLLSSHIFASEEKYGFQIPLYKGEL 162

Query: 190 LDKAIDLGDRLMAAFSSPSGIPYSDVNLGARTAHAPEWSHYSTTAEVTTVQLEFRQLSRA 249
           L  A +L +RL+ AF +P+GIP++ +NL    A+     + +  A  +++ LEF  L+  
Sbjct: 163 LTLATELAERLLPAFRTPTGIPFARINLMKGVAYREVTENCAAAA--SSLVLEFSMLTAL 220

Query: 250 SGNPIFEEAAAAVSEKIHQLPKKHGLVPIFINPNTGHFLPHATITLGARGDSYYEYLLKQ 309
           +GN  F+ +A      + +     GL+   I+  +G ++ +    +GA  DS+YEY  K 
Sbjct: 221 TGNNKFKASAENAFFSVWKRRSGIGLLGNSIDVLSGRWI-YPVSGVGAGIDSFYEYAFKS 279

Query: 310 WLQTGKTINYLLDDYLTGIEGVREFLAKRSSPNKRLFIGELSAGSEAFNPKMDHLTCFLP 369
           ++  G    Y L+ +   +E +R + A   S N+  +    SA     +  +D L+ + P
Sbjct: 280 YIFLGDP-RY-LEVWQKSLESLRHYTA---SLNEYYYQNVYSANGMVMSRWVDSLSAYFP 334

Query: 370 GTLAL-GHLNGLPDWHMTMAEELLYTCYLTYAAHPTFLAPEITHFNLASTTDDMYTKSAD 428
           G L L G L      H+       ++ YL +   P         +NL       YTKS +
Sbjct: 335 GLLVLAGELELAKKMHL-----YYFSIYLKFGQLP-------ERYNL-------YTKSIE 375

Query: 429 AHSL-LRPEFIESLWYMYQITGNTTYQEWGWQIFQGFEKYAKVPNGYSSLTNVKSENPIP 487
            +   LRPEF ES +Y+Y+ T +  Y   G  +    E +   P G++++ N+  E    
Sbjct: 376 LNGYPLRPEFAESTYYLYRATKDVFYLHVGELLLSNIENHLWTPCGFAAVENL--EKYTL 433

Query: 488 RDMMESFFLSETXXXXXXXXSDDRFL-IDLNKYVVNSEAHPLPI 530
            + MESFFLSET         DD  +    + ++  +E H  P+
Sbjct: 434 SNRMESFFLSETLKYLFLLFDDDNPIHRSHHDFIFTTEGHLFPV 477


>SPBC106.01 |mph1|SPBC1271.16c, SPBC243.01|dual specificity protein
           kinase Mph1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 678

 Score = 29.1 bits (62), Expect = 1.6
 Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 2/76 (2%)

Query: 368 LPGTLALGHLNGLPDWHMTMAEELLYTCYLTYAAHPTFLAPEITHFNLASTTDDMYTKSA 427
           +P  L    LN LP +   +A++ L     T  AHP  L+ EI+   L+   +     S 
Sbjct: 594 IPELLVHPFLNPLPSYLTPLAKKPLPVSGHTNNAHPLRLSTEISASQLSMIIERSVELS- 652

Query: 428 DAHSLLRPEFIESLWY 443
             H  L  E I+S+ Y
Sbjct: 653 -KHKRLNKELIDSMAY 667


>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score = 27.5 bits (58), Expect = 4.9
 Identities = 12/34 (35%), Positives = 19/34 (55%)

Query: 285 GHFLPHATITLGARGDSYYEYLLKQWLQTGKTIN 318
           G+ LPHA + L   G    +YL+K  ++ G T +
Sbjct: 168 GYALPHAIMRLDLAGRDLTDYLMKILMERGYTFS 201


>SPBC27B12.11c |||transcription factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 738

 Score = 27.5 bits (58), Expect = 4.9
 Identities = 15/29 (51%), Positives = 17/29 (58%), Gaps = 3/29 (10%)

Query: 376 HLNGLPDWHMTMAEELLYTCYLTYAAHPT 404
           H+N LPD H   AEEL YT  L +   PT
Sbjct: 677 HVNMLPDTH---AEELAYTSMLLFHDIPT 702


>SPBC1347.09 |||hexaprenyldihydroxybenzoate
           methyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 284

 Score = 27.1 bits (57), Expect = 6.5
 Identities = 11/35 (31%), Positives = 20/35 (57%)

Query: 324 YLTGIEGVREFLAKRSSPNKRLFIGELSAGSEAFN 358
           ++  ++ V   L+K   PN RLF+ +L  G + F+
Sbjct: 162 HIKDLQEVTNKLSKLLKPNGRLFVADLIKGGDTFH 196


>SPBC16C6.12c |||Las1-like protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 470

 Score = 27.1 bits (57), Expect = 6.5
 Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%)

Query: 302 YYEYLLKQWLQTGKTINYLLDDYLTGIEGVREFLAKRSSPNKRLFIGELS 351
           +Y YL+++   TG+     L    T +  V E   +RS P  ++ I ELS
Sbjct: 337 WYAYLMRE-AYTGQPWTSTLSVTQTDLASVLEICLQRSDPFTKIIIDELS 385


>SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 825

 Score = 27.1 bits (57), Expect = 6.5
 Identities = 23/75 (30%), Positives = 34/75 (45%), Gaps = 4/75 (5%)

Query: 266 IHQLPKKHGLVPIFINPN--TGHFLPHATITLGARGDSYYEYLLKQWLQTGKTINYLLDD 323
           I + PK  G  P ++N     G F  H      AR D+    LLK WL +    ++ LD+
Sbjct: 324 ILRTPKGKGC-PKYLNGKFLEGTFRAHQVPLKLARTDTNQRNLLKDWLNSYNCQDF-LDE 381

Query: 324 YLTGIEGVREFLAKR 338
           +    +G+ E L  R
Sbjct: 382 HGLPTKGITEHLPPR 396


>SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 365

 Score = 26.6 bits (56), Expect = 8.6
 Identities = 16/49 (32%), Positives = 23/49 (46%), Gaps = 2/49 (4%)

Query: 44  EDSVQEKLRN--TSLNAKPALDNTVQIKYDPTVYERPVFTGPKNDRQRA 90
           E S +E +RN  TSLN +      V ++ DP     P+   P   R R+
Sbjct: 140 EFSTEEDMRNALTSLNGEVIKGQAVTLREDPDAANEPLPEVPSRFRSRS 188


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.319    0.135    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,486,974
Number of Sequences: 5004
Number of extensions: 108217
Number of successful extensions: 255
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 238
Number of HSP's gapped (non-prelim): 9
length of query: 533
length of database: 2,362,478
effective HSP length: 76
effective length of query: 457
effective length of database: 1,982,174
effective search space: 905853518
effective search space used: 905853518
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 56 (26.6 bits)