BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000564-TA|BGIBMGA000564-PA|IPR001382|Glycoside hydrolase, family 47 (533 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein s... 378 e-105 At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mann... 330 2e-90 At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mann... 330 2e-90 At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein s... 156 4e-38 At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein S... 118 1e-26 At4g02405.1 68417.m00325 expressed protein 33 0.47 At1g48390.1 68414.m05405 syntaxin-related family protein contain... 31 1.9 At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (... 31 2.5 At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 29 5.8 At1g26830.1 68414.m03270 cullin, putative similar to Cullin homo... 29 7.7 >At1g30000.1 68414.m03669 glycoside hydrolase family 47 protein similar to GI:5579331 from [Homo sapiens]; contains Pfam profile PF01532: Glycosyl hydrolase family 47 Length = 624 Score = 378 bits (930), Expect = e-105 Identities = 223/499 (44%), Positives = 299/499 (59%), Gaps = 55/499 (11%) Query: 87 RQRALVESFRHAWRGYKEHAWGHDNLKPVSGMASDWFS-LGLTIVDSLDTAYIMGLTDVF 145 RQ+++ E+F HAW GY+++A G+D L P+S D LG T+VD+LDTA IMGL ++ Sbjct: 128 RQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGATVVDALDTAMIMGLDNIV 187 Query: 146 EEGKQWIKEELI--FTKNKDVNFFEVTIRILGGLLTNHYFTQDQ--------------LF 189 E W++ L+ ++ VN FE TIR+LGGLL+ ++ + + ++ Sbjct: 188 SEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSGGEQGTVNMTHVGPKPVIY 247 Query: 190 LDKAIDLGDRLMAAF-SSPSGIPYSDVNLGARTAHAPEWSHYSTTAEVTTVQLEFRQLSR 248 L+ A DL DRL++AF SSP+ +P+ DV L TAH P S+TAEV +VQLEF LS Sbjct: 248 LNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAH-PAPGGASSTAEVASVQLEFNYLSS 306 Query: 249 ASGNPIFEEAAAAVSEKIHQLPKKHGLVPIFINPNTGHFLPHATITLGARGDSYYEYLLK 308 SG+P + A V I LPK GLVPI+I+P TG F+ I LG+RGDSYYEYL+K Sbjct: 307 ISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGE-NIRLGSRGDSYYEYLIK 365 Query: 309 QWLQTGKTIN----YLLDDYLTGIEGVREFLAKRSSPNKRLFIGELSAGSEA-FNPKMDH 363 WLQ G +N YL D Y+ ++GVR L + S P +F+GEL GS+ F+PKMDH Sbjct: 366 VWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVGELPYGSKGEFSPKMDH 425 Query: 364 LTCFLPGTLALGHLNGLPD--------------WHMTMAEELLYTCYLTYAAHPTFLAPE 409 L CFLPGTLALG GL ++ +AE+L TC+ Y T LAPE Sbjct: 426 LVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAKTCFEMYEVTATGLAPE 485 Query: 410 ITHFNLASTTDD---------MYT-----KSADAHSLLRPEFIESLWYMYQITGNTTYQE 455 I +F+ T+D MY K AD H+LLRPE +ESL+ +Y+IT +T Y++ Sbjct: 486 IAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVESLFVLYRITKDTKYRD 545 Query: 456 WGWQIFQGFEKYAKVPN-GYSSLTNVKSENPIPRDMMESFFLSETXXXXXXXXSDDRFLI 514 GWQIF+ FEKY KV + GY+SL +V P RD ME+FFL ET DD +I Sbjct: 546 QGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGETLKYLYLLFGDDS-VI 604 Query: 515 DLNKYVVNSEAHPLPIHKN 533 L+K+V N+EAHPLPI +N Sbjct: 605 PLDKFVFNTEAHPLPIRRN 623 >At3g21160.1 68416.m02673 mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative similar to mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine max][GI:6552504] Length = 572 Score = 330 bits (810), Expect = 2e-90 Identities = 194/492 (39%), Positives = 290/492 (58%), Gaps = 24/492 (4%) Query: 50 KLRNTSLNAKPALDNTVQIKYDPTVYE-RPVFTGPKN-DRQRALVESFRHAWRGYKEHAW 107 KL L + L+ + D +V + V P + R + + E+ HAW Y+++AW Sbjct: 59 KLNEEVLRLQQMLEEIKSVTEDVSVNSLKDVQEDPVDAQRMQRVKEAMVHAWSSYEKYAW 118 Query: 108 GHDNLKPVSGMASDWFS-LGLTIVDSLDTAYIMGLTDVFEEGKQWIKEELIFTKNKDVNF 166 G D L+P + D F LG T++D+LDT YIMGL + F++ ++W+ L F K+ + Sbjct: 119 GQDELQPQTKDGVDSFGGLGATMIDALDTLYIMGLDEQFQKAREWVASSLDFDKDYAASM 178 Query: 167 FEVTIRILGGLLTNHYFTQDQLFLDKAIDLGDRLMAAFSSPSGIPYSDVNLGARTAHAPE 226 FE TIR++GGLL+ + + D++FL+KA+D+ DRL+ A+ + SGIPY+ +NL AH P Sbjct: 179 FETTIRVVGGLLSAYDLSGDKIFLEKAMDIADRLLPAWDTQSGIPYNIINLKHGNAHNPT 238 Query: 227 WS-HYSTTAEVTTVQLEFRQLSRASGNPIFEEAAAAVSEKIHQLPKKHGLVPIFINPNTG 285 W+ S A+ T QLEF LS+ +G+P +++ V +++ GL+PI+INP+T Sbjct: 239 WAGGDSILADSGTEQLEFIALSQRTGDPKYQQKVEKVISVLNKNFPADGLLPIYINPDTA 298 Query: 286 HFLPHATITLGARGDSYYEYLLKQWLQTGKT--INYLLDDYLTGIEGVREFLAKRSSPNK 343 + +TIT GA GDS+YEYLLK W+ KT + + D + + G+ L K+S+P Sbjct: 299 N-PSQSTITFGAMGDSFYEYLLKVWVFGNKTSAVKHYRDMWEKSMNGLLS-LVKKSTPLS 356 Query: 344 RLFIGELSAGSEAFNPKMDHLTCFLPGTLALGHLNGLPD-----WHMTMAEELLYTCYLT 398 +I E S S KMD L CF PG LALG +G D +T+AEEL +TCY Sbjct: 357 FTYICEKSGNS--LIDKMDELACFAPGMLALG-ASGYSDPAEGKKFLTLAEELAWTCYNF 413 Query: 399 YAAHPTFLAPEITHFNLASTTDDMYTKSADAHSLLRPEFIESLWYMYQITGNTTYQEWGW 458 Y + PT LA E FN S DM + ++LRPE +ESL+Y++++TGN TYQEWGW Sbjct: 414 YQSTPTKLAGENYFFNSGS---DM--SVGTSWNILRPETVESLFYLWRLTGNKTYQEWGW 468 Query: 459 QIFQGFEKYAKVPNGYSSLTNVKSENPIPRDMMESFFLSETXXXXXXXXSDDRFLIDLNK 518 IF+ FEK +++ +GY L +V + + + M+SFFL+ET S +I L++ Sbjct: 469 NIFEAFEKNSRIESGYVGLKDVNT--GVKDNKMQSFFLAETLKYLYLLFSPTT-VIPLDE 525 Query: 519 YVVNSEAHPLPI 530 +V N+EAHPL I Sbjct: 526 WVFNTEAHPLKI 537 >At1g51590.1 68414.m05808 mannosyl-oligosaccharide 1,2-alpha-mannosidase, putative similar to mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine max][GI:6552504] Length = 560 Score = 330 bits (810), Expect = 2e-90 Identities = 185/452 (40%), Positives = 280/452 (61%), Gaps = 20/452 (4%) Query: 87 RQRALVESFRHAWRGYKEHAWGHDNLKPVSGMASDWFS-LGLTIVDSLDTAYIMGLTDVF 145 R++ + E+ HAW Y+++AWG D L+P + +D F LG T+VDSLDT YIMGL + F Sbjct: 97 RRQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQF 156 Query: 146 EEGKQWIKEELIFTKNKDVNFFEVTIRILGGLLTNHYFTQDQLFLDKAIDLGDRLMAAFS 205 ++ ++W+ L F K+ D + FE TIR++GGLL+ + + D++FL+KA D+ DRL+ A++ Sbjct: 157 QKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLSGDKMFLEKAKDIADRLLPAWN 216 Query: 206 SPSGIPYSDVNLGARTAHAPEWS--HYSTTAEVTTVQLEFRQLSRASGNPIFEEAAAAVS 263 +P+GIPY+ +NL AH P W+ S A+ T QLEF LS+ +G+P +++ V Sbjct: 217 TPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALSQRTGDPKYQQKVEKVI 276 Query: 264 EKIHQLPKKHGLVPIFINPNTGHFLPHATITLGARGDSYYEYLLKQWLQTGKT--INYLL 321 ++++ GL+PI+INP+ + ++T T GA GDS+YEYLLK W+Q KT + Sbjct: 277 TELNKNFPADGLLPIYINPDNAN-PSYSTTTFGAMGDSFYEYLLKVWVQGNKTSAVKPYR 335 Query: 322 DDYLTGIEGVREFLAKRSSPNKRLFIGELSAGSEAFNPKMDHLTCFLPGTLALGHLNGLP 381 D + ++G+ L K+S+P+ +I E + G+ + KMD L CF PG LALG P Sbjct: 336 DMWEKSMKGLLS-LVKKSTPSSFTYICEKN-GNNLID-KMDELACFAPGMLALGASGYGP 392 Query: 382 DWH---MTMAEELLYTCYLTYAAHPTFLAPEITHFNLASTTDDMYTKSADAHSLLRPEFI 438 D +++A EL +TCY Y + PT LA E F + DM + ++LRPE + Sbjct: 393 DEEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFF---TAGQDM--SVGTSWNILRPETV 447 Query: 439 ESLWYMYQITGNTTYQEWGWQIFQGFEKYAKVPNGYSSLTNVKSENPIPRDMMESFFLSE 498 ESL+Y++++TGN TYQEWGW IFQ FEK ++V +GY L +V + + M+SFFL+E Sbjct: 448 ESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNT--GAKDNKMQSFFLAE 505 Query: 499 TXXXXXXXXSDDRFLIDLNKYVVNSEAHPLPI 530 T S +I L+++V N+EAHPL I Sbjct: 506 TLKYLYLLFSPSS-VISLDEWVFNTEAHPLKI 536 >At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein similar to mannosyl-oligosaccharide 1,2-alpha-mannosidase IB [Mus musculus][SP|P39098] Length = 624 Score = 156 bits (378), Expect = 4e-38 Identities = 142/455 (31%), Positives = 208/455 (45%), Gaps = 48/455 (10%) Query: 95 FRHAWRGYKEHAWGHDNLKPVSGMASDWFS-LGLTIVDSLDTAYIMGLTDVFEEGKQWIK 153 F HA+ GY +A+ D L+P+S D LT++DSLDT ++G + F +WI Sbjct: 48 FYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTLIDSLDTLALLGDRERFTSSVEWIG 107 Query: 154 EELIFTKNKDVNFFEVTIRILGGLLTNHYFTQDQL-----------FLDKAIDLGDRLMA 202 + L F NK V+ FE TIR+LGGLL+ H D L A +L R++ Sbjct: 108 KNLQFNINKTVSVFETTIRVLGGLLSAHLIASDYATGMRIPSYNNELLVLAENLARRMLP 167 Query: 203 AFSSPSGIPYSDVNLGARTAHAPEWSHYSTTAEVTTVQLEFRQLSRASGNPIFEEAAAAV 262 AF +P+GIP+ VNL S ++TA T+ LEF LSR + +P+FE+ A Sbjct: 168 AFDTPTGIPFGSVNL--MYGVDKHESKITSTAGGGTLSLEFGVLSRLTNDPVFEQVAKNA 225 Query: 263 SEKIHQLPKKHGLVPIFINPNTGHFLPHATITLGARGDSYYEYLLKQWLQTGKTINYLLD 322 + LV IN TG + +G DS+YEYLLK ++ G + Sbjct: 226 VRGLWARRSNLDLVGAHINVFTGEW-TQKDAGIGTSIDSFYEYLLKAYILFGD------E 278 Query: 323 DYLTGIEGVREFLAKRSSPNKRLFIGELSAGSEAFN-PKMDHLTCFLPGTLAL-GHLNGL 380 +YL + + + +K + E++ S A P + L F PG L G ++ Sbjct: 279 EYLYIFQ--EAYRSAMQYLHKDPWYVEVNMDSAAIVWPVFNSLQAFWPGLQVLAGDVDPA 336 Query: 381 PDWHMTMAEELLYTCYLTYAAHPTFLAPEITHFNLASTTDDMYTKSADAHSLLRPEFIES 440 H ++ + Y PE FNLA+ + KS LRPE IES Sbjct: 337 IRTHTA-----FFSVWKRYG-----FTPE--GFNLATLSVQYGQKSYP----LRPELIES 380 Query: 441 LWYMYQITGNTTYQEWGWQIFQGFEKYAKVPNGYSSLTNVKSENPIPRDMMESFFLSETX 500 +++Y+ T + Y + G + AK P GY +T+V+ D MESFFL+ET Sbjct: 381 TYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDVELHK--QEDHMESFFLAETV 438 Query: 501 X---XXXXXXSDDRFLIDLN--KYVVNSEAHPLPI 530 D L+D KY+ ++E H LPI Sbjct: 439 KYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPI 473 >At1g27520.1 68414.m03355 glycoside hydrolase family 47 protein Similar to gb|U04299 mannosyl-oligosaccharide alpha-1,2-mannosidase from Mus musculus. ESTs gb|R84145 and gb|AA394707 come from this gene Length = 574 Score = 118 bits (283), Expect = 1e-26 Identities = 82/259 (31%), Positives = 134/259 (51%), Gaps = 30/259 (11%) Query: 84 KNDRQRALVESFRHAWRGYKEHAWGHDNLKPVSGMASDWFS-LG---------------L 127 K + + E F HA+ Y +A+ HD LKP++ +D S LG + Sbjct: 34 KKQMREKVREMFYHAYDNYMTYAFPHDELKPLTKSFTDSLSELGNLKLEHLPTDYNGSAV 93 Query: 128 TIVDSLDTAYIMGLTDVFEEGKQWIKEELIFTKNKDVNFFEVTIRILGGLLTNHYFTQD- 186 T+V+SL + I+G + FE+G W+ E L F + VN FE IR+LGGL++ H D Sbjct: 94 TLVESLSSLAILGNSTEFEKGVLWLSENLTFDIDARVNLFECNIRVLGGLISAHLLAIDP 153 Query: 187 ----------QLFLDKAIDLGDRLMAAFSSPSGIPYSDVNLGARTAHAPEWSHYSTTAEV 236 L A DLG R + AF +P+G+PY+ +NL + + ++T+ Sbjct: 154 NNRLIQGSYNNQLLRLAEDLGKRFLPAFETPTGLPYAWINL--KNGVMENETTETSTSGC 211 Query: 237 TTVQLEFRQLSRASGNPIFEEAAAAVSEKIHQLPKKHGLVPIFINPNTGHFLPHATITLG 296 ++ LE LSR +G+P FE AA ++ ++ L+ ++ TG ++ +++ ++G Sbjct: 212 GSLVLEMGALSRLTGDPRFESAALRALRQLWRMRSSLDLLGTTLDVVTGEWIEYSS-SIG 270 Query: 297 ARGDSYYEYLLKQWLQTGK 315 A DS+YEYLLK ++ GK Sbjct: 271 AGVDSFYEYLLKAYILFGK 289 Score = 59.7 bits (138), Expect = 5e-09 Identities = 34/98 (34%), Positives = 51/98 (52%), Gaps = 4/98 (4%) Query: 433 LRPEFIESLWYMYQITGNTTYQEWGWQIFQGFEKYAKVPNGYSSLTNVKSENPIPRDMME 492 LRPE ES +Y+YQ T + Y + G + + Y KVP G++S+ +V + D Sbjct: 385 LRPELAESTFYLYQATKDPWYLDVGESMVKSLNLYTKVPGGFASVRDVTTMQ--LEDHQH 442 Query: 493 SFFLSETXXXXXXXXSDDRFLIDLNKYVVNSEAHPLPI 530 SFFL+ET DD F+ N Y+ +E HP+ + Sbjct: 443 SFFLAETCKYLYLLF-DDSFVAKRN-YIFTTEGHPIQV 478 >At4g02405.1 68417.m00325 expressed protein Length = 333 Score = 33.1 bits (72), Expect = 0.47 Identities = 27/87 (31%), Positives = 39/87 (44%), Gaps = 6/87 (6%) Query: 208 SGIPYSDVNLGARTAHAPEWSHYSTTA--EVTTVQLEFRQLSRASGNPIFEEAAAAVSEK 265 S S ++L ++ AP H+ TA T L R L RA P + A + S Sbjct: 16 SSFKKSAISLRMQSLCAPPPVHFPATAIRRCRTPTLAVRSL-RAENEPSIQSALVSAS-- 72 Query: 266 IHQLPKKHGLVPIFINPNTGHFLPHAT 292 H+L + + P+FI+P FLP T Sbjct: 73 -HRLRETNRTEPLFIDPYAACFLPPYT 98 >At1g48390.1 68414.m05405 syntaxin-related family protein contains a novel domain similar to F-box that is shared among other proteins in Arabidopsis; similar to proteins At3g54160, At1g47920 (syntaxin SYP81), At5g41830, At3g44180, At3g58890, At1g56610, At1g48390, At3g59270 [Arabidopsis thaliana] Length = 413 Score = 31.1 bits (67), Expect = 1.9 Identities = 23/94 (24%), Positives = 42/94 (44%), Gaps = 4/94 (4%) Query: 403 PTFLAPEITHFNLASTTDDMYTKSADAHSLLRPEFIESLWYMYQITGNTTYQE--WGWQI 460 P F+AP + + + +S + Y D +SL+ L + G++ +GW Sbjct: 185 PCFIAPSLVYLDYSSYLSENYDL-IDLNSLVEARLNLRLLESFNNYGSSDNDGDVYGWDF 243 Query: 461 FQGFEKYAKVPNGYSSLTNVKSENPIPRDMMESF 494 F+ Y V N + +TN+ + + P D +E F Sbjct: 244 NVPFDYYGDVTNLVAGITNITTLHLSP-DSLEVF 276 >At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (Atm) identical to ataxia-telangiectasia mutated protein (Atm) [Arabidopsis thaliana] GI:7529272; contains Pfam profile PF00855: PWWP domain; contains GA donor splice site at exon 73 Length = 3255 Score = 30.7 bits (66), Expect = 2.5 Identities = 18/59 (30%), Positives = 25/59 (42%), Gaps = 1/59 (1%) Query: 91 LVESFRHAWRGYKEHAWG-HDNLKPVSGMASDWFSLGLTIVDSLDTAYIMGLTDVFEEG 148 L+ SF + Y EHAW H + P +A D +G + LD A D+ G Sbjct: 1609 LLRSFAKVYEEYTEHAWNTHSDTVPSKSLAPDSPEVGRIVDMDLDLAEDTKERDIIAAG 1667 >At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein / eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1 domain 3 Length = 434 Score = 29.5 bits (63), Expect = 5.8 Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 2/57 (3%) Query: 132 SLDTA-YIMGLTDVFEEGKQWIKEELIFTKNKDVNFFEVTIRILGGLLTNHYFTQDQ 187 S DT Y+ G+ D + + E LI +N D+N +E+ G ++ H F +DQ Sbjct: 294 SQDTGKYVFGVEDTLKALEMGAIETLIVWENLDINRYELKNSTTGEMVVKH-FGKDQ 349 >At1g26830.1 68414.m03270 cullin, putative similar to Cullin homolog 3 (CUL-3) SP:Q13618, GI:3639052 from [Homo sapiens]; contains Pfam profile PF00888: Cullin family Length = 732 Score = 29.1 bits (62), Expect = 7.7 Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 2/74 (2%) Query: 249 ASGNPIFEEAAAAVSEKIHQLPKKHGLVPIFINPNTGHFLPHATITLGARGDSYYEYLLK 308 ASG E A + +H+ +K L FI T H + + A+G S+ E L K Sbjct: 44 ASGLSFEELYRNAYNMVLHKFGEK--LYTGFIATMTSHLKEKSKLIEAAQGGSFLEELNK 101 Query: 309 QWLQTGKTINYLLD 322 +W + K + + D Sbjct: 102 KWNEHNKALEMIRD 115 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.319 0.135 0.412 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,448,049 Number of Sequences: 28952 Number of extensions: 528999 Number of successful extensions: 1165 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 1122 Number of HSP's gapped (non-prelim): 15 length of query: 533 length of database: 12,070,560 effective HSP length: 84 effective length of query: 449 effective length of database: 9,638,592 effective search space: 4327727808 effective search space used: 4327727808 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 62 (29.1 bits)
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