Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000559-TA|BGIBMGA000559-PA|IPR000652|Triosephosphate
isomerase
         (174 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic, p...   180   5e-46
At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,...   177   3e-45
At2g16750.1 68415.m01921 protein kinase family protein contains ...    31   0.43 
At5g57390.1 68418.m07170 ovule development protein, putative sim...    28   4.0  
At5g25630.1 68418.m03050 pentatricopeptide (PPR) repeat-containi...    27   5.3  
At3g11490.1 68416.m01401 rac GTPase activating protein, putative...    27   5.3  
At2g29060.1 68415.m03532 scarecrow transcription factor family p...    27   5.3  
At3g24710.1 68416.m03102 hypothetical protein                          27   7.0  
At3g22670.1 68416.m02861 pentatricopeptide (PPR) repeat-containi...    27   7.0  
At5g55520.1 68418.m06915 expressed protein weak similarity to ph...    27   9.2  
At5g41220.1 68418.m05009 glutathione S-transferase, putative sim...    27   9.2  
At5g21222.1 68418.m02532 protein kinase family protein contains ...    27   9.2  
At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase, put...    27   9.2  
At1g01130.1 68414.m00016 expressed protein ; expression supporte...    27   9.2  

>At3g55440.1 68416.m06157 triosephosphate isomerase, cytosolic,
           putative strong similarity to triosephosphate isomerase,
           cytosolic from Petunia hybrida [SP|P48495], from Coptis
           japonica [SP|P21820]
          Length = 254

 Score =  180 bits (438), Expect = 5e-46
 Identities = 84/136 (61%), Positives = 102/136 (75%), Gaps = 1/136 (0%)

Query: 37  EVAHALESGLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLAYEPVWAI 96
           +VA+AL  GLKVIAC+GETLEERE+G T +VV  Q KA+   + + W N+V+AYEPVWAI
Sbjct: 113 KVAYALAQGLKVIACVGETLEEREAGSTMDVVAAQTKAIADRVTN-WSNVVIAYEPVWAI 171

Query: 97  GTGKTATPQQAQDVHHALRNWLSANVSGSVSDAVRIQYGGSVTAANAKELASCKDIDGFL 156
           GTGK A+P QAQ+VH  LR WL+ NVS  V+   RI YGGSV   N KEL    D+DGFL
Sbjct: 172 GTGKVASPAQAQEVHDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGFL 231

Query: 157 VGGASLKPEFVEIVNA 172
           VGGASLKPEF++I+ A
Sbjct: 232 VGGASLKPEFIDIIKA 247



 Score = 42.7 bits (96), Expect = 1e-04
 Identities = 19/44 (43%), Positives = 27/44 (61%), Gaps = 1/44 (2%)

Query: 1  MGRKFVVGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVAHALES 44
          M RKF VGGNWK NG   ++ +IVN L +  + P+ +V   + S
Sbjct: 1  MARKFFVGGNWKCNGTAEEVKKIVNTLNEAQV-PSQDVVEVVVS 43


>At2g21170.1 68415.m02511 triosephosphate isomerase, chloroplast,
           putative similar to Triosephosphate isomerase,
           chloroplast precursor: SP|P48496 from Spinacia oleracea,
           SP|P46225 from Secale cereale
          Length = 315

 Score =  177 bits (432), Expect = 3e-45
 Identities = 89/137 (64%), Positives = 103/137 (75%), Gaps = 2/137 (1%)

Query: 37  EVAHALESGLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLAYEPVWAI 96
           + A+AL  GL VIACIGE LEERE+GKT +V F QLKA   A+   W+NIV+AYEPVWAI
Sbjct: 172 KAAYALSEGLGVIACIGEKLEEREAGKTFDVCFAQLKAFADAV-PSWDNIVVAYEPVWAI 230

Query: 97  GTGKTATPQQAQDVHHALRNWLSANVSGSVSDAVRIQYGGSVTAANAKELASCKDIDGFL 156
           GTGK A+PQQAQ+VH A+R WL  NVS  V+   RI YGGSV   N+ ELA  +DIDGFL
Sbjct: 231 GTGKVASPQQAQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFL 290

Query: 157 VGGASLK-PEFVEIVNA 172
           VGGASLK PEF  IVN+
Sbjct: 291 VGGASLKGPEFATIVNS 307



 Score = 41.5 bits (93), Expect = 3e-04
 Identities = 16/35 (45%), Positives = 25/35 (71%)

Query: 4  KFVVGGNWKMNGDKNQINEIVNNLKKGPLDPNVEV 38
          KF VGGNWK NG K+ I +++++L    L+ +V+V
Sbjct: 65 KFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDV 99


>At2g16750.1 68415.m01921 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain
          Length = 617

 Score = 31.1 bits (67), Expect = 0.43
 Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 2/69 (2%)

Query: 18  NQINEIVNNLKKGPLDPNVEVAHAL--ESGLKVIACIGETLEERESGKTEEVVFRQLKAL 75
           N +  + N   KG L+  ++  H L  E  LK+   +GE L+   +  +  V+ R +K+ 
Sbjct: 344 NDLISVYNLSSKGSLEETLQGKHVLRWEERLKIAIGLGEALDYLHNQCSNPVIHRDVKSS 403

Query: 76  VSAIGDKWE 84
              + D++E
Sbjct: 404 NVLLSDEFE 412


>At5g57390.1 68418.m07170 ovule development protein, putative
           similar to ovule development protein AINTEGUMENTA
           (GI:1209099)[Arabidopsis thaliana]
          Length = 555

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 15/49 (30%), Positives = 21/49 (42%)

Query: 81  DKWENIVLAYEPVWAIGTGKTATPQQAQDVHHALRNWLSANVSGSVSDA 129
           D + N   A  PV AI    T T     + +    NW++ N+S S   A
Sbjct: 492 DGYNNHSAASNPVSAIPLSSTTTMSNGNEGYGGNINWINNNISSSYQTA 540


>At5g25630.1 68418.m03050 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 574

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 37/153 (24%), Positives = 60/153 (39%), Gaps = 23/153 (15%)

Query: 6   VVGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVAHALESGLKVIACIGETLEERESGKTE 65
           V+ G W  NG  +    + N + K  + PN++    L  G          LE ++  K E
Sbjct: 405 VISG-WCSNGSMDDAMRVFNKMCKFGVSPNIKTFETLMWGY---------LEVKQPWKAE 454

Query: 66  EVVFRQLKALVSAIGDKWEN-IVLAYEPVWAIG-----TGKTATPQQAQDVHHA-LRNWL 118
           EV+      ++   G K EN   L     W +      + K     + +D+  A L    
Sbjct: 455 EVL-----QMMRGCGVKPENSTFLLLAEAWRVAGLTDESNKAINALKCKDIEIAKLEKLY 509

Query: 119 SANVSGSVSDAVRIQYG-GSVTAANAKELASCK 150
               SGS  + ++I  G   +  A A  L++CK
Sbjct: 510 QKQSSGSSFNLLQIPVGKRELPTAKAMNLSACK 542


>At3g11490.1 68416.m01401 rac GTPase activating protein, putative
           similar to rac GTPase activating protein 1 GB:AAC62624
           GI:3695059 [Lotus japonicus]; contains Pfam profile
           PF00620: RhoGAP domain
          Length = 435

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 15/41 (36%), Positives = 23/41 (56%), Gaps = 1/41 (2%)

Query: 7   VGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVAHALESGLK 47
           V G +++NG+  Q   I   L KG +  N++V H L S +K
Sbjct: 173 VEGIFRINGENGQEEYIREELNKGIIPDNIDV-HCLASLIK 212


>At2g29060.1 68415.m03532 scarecrow transcription factor family
            protein 
          Length = 1336

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 13/48 (27%), Positives = 23/48 (47%)

Query: 43   ESGLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKWENIVLAY 90
            ++G ++  C    LE R  G T  ++     AL S++ D   + + AY
Sbjct: 990  DAGQRLAHCFANALEARLQGSTGPMIQTYYNALTSSLKDTAADTIRAY 1037


>At3g24710.1 68416.m03102 hypothetical protein
          Length = 126

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 14/31 (45%), Positives = 22/31 (70%), Gaps = 1/31 (3%)

Query: 48 VIACIGETLEERESGKTEEVVFRQLKALVSA 78
          V++ +GE + E E+G ++EV  R+LKAL  A
Sbjct: 28 VMSALGEIVSE-ETGGSKEVASRRLKALEKA 57


>At3g22670.1 68416.m02861 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 562

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 15/59 (25%), Positives = 29/59 (49%), Gaps = 4/59 (6%)

Query: 15  GDKNQINEIVNNLKKGPLDPNVE----VAHALESGLKVIACIGETLEERESGKTEEVVF 69
           GD  ++NE++  +++   +PNV     V H+L    +V   +G   + +E G   +  F
Sbjct: 322 GDFRRVNEMLEEMRENGCNPNVVTYTIVMHSLGKSKQVAEALGVYEKMKEDGCVPDAKF 380


>At5g55520.1 68418.m06915 expressed protein weak similarity to
           phragmoplast-associated kinesin-related protein 1
           [Arabidopsis thaliana] GI:8745333; expression supported
           by MPSS
          Length = 802

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 12/45 (26%), Positives = 20/45 (44%)

Query: 7   VGGNWKMNGDKNQINEIVNNLKKGPLDPNVEVAHALESGLKVIAC 51
           V  + K       + E  +   + PL P   +A +L+ GL +I C
Sbjct: 288 VAASTKFQASPRNVTESSSTGNRKPLSPTDSLAASLQRGLNIIDC 332


>At5g41220.1 68418.m05009 glutathione S-transferase, putative
           similar to emb|CAA10662
          Length = 590

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 16/61 (26%), Positives = 27/61 (44%), Gaps = 1/61 (1%)

Query: 11  WKMNGDK-NQINEIVNNLKKGPLDPNVEVAHALESGLKVIACIGETLEERESGKTEEVVF 69
           WK  G   N    + N  K+ P       +   +   K + C  + L +R SG++E+ VF
Sbjct: 304 WKRIGAYFNNSASLANLPKREPSHCKQRWSKLNDKVCKFVGCYDQALNQRSSGQSEDDVF 363

Query: 70  R 70
           +
Sbjct: 364 Q 364


>At5g21222.1 68418.m02532 protein kinase family protein contains
           Pfam profile: PF00069 protein kinase domain
          Length = 831

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 10/35 (28%), Positives = 17/35 (48%)

Query: 11  WKMNGDKNQINEIVNNLKKGPLDPNVEVAHALESG 45
           W   GD  +  EI  ++ +G +DP++     L  G
Sbjct: 611 WSSVGDMKRCEEIYTDMLEGGIDPDIHAFSILAKG 645


>At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase,
           putative similar to glycerol-3-phosphate dehydrogenase
           GB:BAA08926 from Mus musculus [SP|Q64521], Homo sapiens
           [GI:1020315], Rattus norvegicus [SP|P35571]; contains
           Pfam profile PF01266 FAD dependent oxidoreductase
          Length = 629

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)

Query: 44  SGLKVIACIGETLEERESGKTEEVVFRQLKALVSAIGDKW 83
           SG++ +A +  T +  ES   + VVF +   LV+  G KW
Sbjct: 401 SGIRPLA-MDPTAKSTESISRDHVVFEENPGLVTITGGKW 439


>At1g01130.1 68414.m00016 expressed protein ; expression supported
           by MPSS
          Length = 180

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 3/43 (6%)

Query: 8   GGNWKMNGDKNQINEIVNNLKKGPLDPNVEVAHAL--ESGLKV 48
           GG WK++G KN+ N+     +K  + P  E+  AL   SG KV
Sbjct: 81  GGKWKVDGRKNK-NKKEKEKEKEEIIPFKEIIEALLGNSGDKV 122


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.313    0.131    0.382 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,178,026
Number of Sequences: 28952
Number of extensions: 163065
Number of successful extensions: 362
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 351
Number of HSP's gapped (non-prelim): 17
length of query: 174
length of database: 12,070,560
effective HSP length: 76
effective length of query: 98
effective length of database: 9,870,208
effective search space: 967280384
effective search space used: 967280384
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 56 (26.6 bits)