Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000548-TA|BGIBMGA000548-PA|IPR006968|Protein of unknown
function DUF647
(422 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13770.1 68414.m01616 expressed protein contains Pfam domain,... 119 3e-27
At3g45890.1 68416.m04966 expressed protein contains Pfam domain,... 108 6e-24
At5g01510.1 68418.m00065 expressed protein contains Pfam domain,... 87 2e-17
At2g31190.1 68415.m03809 expressed protein contains Pfam domain,... 79 7e-15
At5g49820.1 68418.m06170 expressed protein contains Pfam domain,... 77 2e-14
At2g23470.1 68415.m02802 expressed protein contains Pfam domain,... 48 1e-05
At5g14220.1 68418.m01661 protoporphyrinogen oxidase, putative si... 33 0.27
At4g13650.1 68417.m02123 pentatricopeptide (PPR) repeat-containi... 31 1.4
At4g27500.1 68417.m03950 expressed protein non-consensus GA dono... 31 1.9
At3g02020.1 68416.m00164 aspartate kinase, lysine-sensitive, put... 31 1.9
At1g52620.1 68414.m05941 pentatricopeptide (PPR) repeat-containi... 30 2.5
At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF051... 30 3.3
At4g30960.1 68417.m04395 CBL-interacting protein kinase 6 (CIPK6... 29 4.4
At3g23000.1 68416.m02900 CBL-interacting protein kinase 7 (CIPK7... 29 4.4
At2g32700.5 68415.m04001 WD-40 repeat family protein contains 7 ... 29 4.4
At2g32700.4 68415.m04000 WD-40 repeat family protein contains 7 ... 29 4.4
At2g32700.3 68415.m03999 WD-40 repeat family protein contains 7 ... 29 4.4
At2g32700.2 68415.m03998 WD-40 repeat family protein contains 7 ... 29 4.4
At2g32700.1 68415.m03997 WD-40 repeat family protein contains 7 ... 29 4.4
At1g56660.1 68414.m06516 expressed protein 29 4.4
At5g21222.1 68418.m02532 protein kinase family protein contains ... 29 7.7
>At1g13770.1 68414.m01616 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647; contains
non-consensus (GC) splice site at intron 2
Length = 440
Score = 119 bits (287), Expect = 3e-27
Identities = 53/142 (37%), Positives = 86/142 (60%)
Query: 9 KINYFRNWFTNVFLPKGYPDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVGDT 68
+ N+ F+P+G+P SV+ DY +Q+WDT Q + L+TQ +L +GVG+
Sbjct: 45 RFNHVWRRVLQAFVPEGFPGSVTPDYVGFQLWDTLQGLSTYTKMMLSTQALLSAIGVGEK 104
Query: 69 TATPLAATVTWVIKDGCGHLGRILFAFTHGTCLDAYSKKWRLYADILNDAAMCIEIALPY 128
+AT + AT W ++D G LG ILF F G+ LD+ +K WRL AD++ND M +++ P
Sbjct: 105 SATVIGATFQWFLRDFTGMLGGILFTFYQGSNLDSNAKMWRLVADLMNDIGMLMDLLSPL 164
Query: 129 FKNYTTVVLCVSTVMKAIVGVS 150
F + VV+C+ ++ ++ GV+
Sbjct: 165 FPSAFIVVVCLGSLSRSFTGVA 186
>At3g45890.1 68416.m04966 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647
Length = 608
Score = 108 bits (260), Expect = 6e-24
Identities = 51/123 (41%), Positives = 75/123 (60%), Gaps = 2/123 (1%)
Query: 14 RNWFTNVFLPKGYPDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVGDTTATPL 73
RN T LP+G+P+SV+ DY Y +W Q S I+G LATQ +L VG+G A P
Sbjct: 191 RNLLTQFLLPEGFPNSVTSDYLDYSLWRGVQGIASQISGVLATQSLLYAVGLG-KGAIPT 249
Query: 74 AATVTWVIKDGCGHLGRILFAFTHGTCLDAYSKKWRLYADILNDAAMCIEIALPYFKNYT 133
AA + WV+KDG G+L +I+ + +G D + K WRL+AD+L +AA +E+ P F +
Sbjct: 250 AAAINWVLKDGIGYLSKIMLS-KYGRHFDVHPKGWRLFADLLENAAFGMEMLTPVFPQFF 308
Query: 134 TVV 136
++
Sbjct: 309 VMI 311
>At5g01510.1 68418.m00065 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647
Length = 509
Score = 87.4 bits (207), Expect = 2e-17
Identities = 49/153 (32%), Positives = 72/153 (47%), Gaps = 8/153 (5%)
Query: 7 ETKINYFRNWFTNVFLPKGYPDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVG 66
ET I + + + P G+P SVS DY Y +W I L T +L+ VGVG
Sbjct: 105 ETNILWLPDVVRDFVFPSGFPGSVSDDYLDYMLWQFPTNITGWICNVLVTSSLLKAVGVG 164
Query: 67 DTTATPLAAT-------VTWVIKDGCGHLGRILFAFTHGTCLDAYSKKWRLYADILNDAA 119
+ T AAT + WV KDG G LGR+L G+ D K+WR+YAD + A
Sbjct: 165 SFSGTSAAATAAASAAAIRWVSKDGIGALGRLLIGGRFGSLFDDDPKQWRMYADFIGSAG 224
Query: 120 MCIEIALPYFKNYTTVVLCVSTVMKAIV-GVSD 151
++A + + ++ + KA+ G+ D
Sbjct: 225 SFFDLATQLYPSQFLLLASTGNLAKAVARGLRD 257
>At2g31190.1 68415.m03809 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647
Length = 433
Score = 78.6 bits (185), Expect = 7e-15
Identities = 40/111 (36%), Positives = 60/111 (54%), Gaps = 3/111 (2%)
Query: 17 FTNVFLPKGYPDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVGDTTATPLAAT 76
F N F P GYP SV+ Y Y + Q F S L+TQ +L G+ T A A
Sbjct: 67 FLNKFFPSGYPYSVNEGYLRYTQFRALQHFSSAALSVLSTQSLLFAAGLRPTPAQ--ATV 124
Query: 77 VTWVIKDGCGHLGRILFAFTHGTCLDAYSKKWRLYADILNDAAMCIEIALP 127
V+W++KDG H+G+++ + G +D+ K+WR+ AD+L D +E+ P
Sbjct: 125 VSWILKDGMQHVGKLICS-NLGARMDSEPKRWRILADVLYDLGTGLELVSP 174
>At5g49820.1 68418.m06170 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647
Length = 497
Score = 77.4 bits (182), Expect = 2e-14
Identities = 38/144 (26%), Positives = 68/144 (47%), Gaps = 1/144 (0%)
Query: 7 ETKINYFRNWFTNVFLPKGYPDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVG 66
+T + ++ + +P+G+P SV+ Y Y W + F G TQ +L VG
Sbjct: 98 QTPFDEVGSFLRSYVVPEGFPGSVNESYVPYMTWRALKHFFGGAMGVFTTQTLLNSVGAS 157
Query: 67 DTTATPLAATVTWVIKDGCGHLGRILFAFTHGTCLDAYSKKWRLYADILNDAAMCIEIAL 126
++ A + W++KDG G +G++LFA G D K+ R D+L + +E+A
Sbjct: 158 RNSSASAAVAINWILKDGAGRVGKMLFA-RQGKKFDYDLKQLRFAGDLLMELGAGVELAT 216
Query: 127 PYFKNYTTVVLCVSTVMKAIVGVS 150
+ + C + V+K + V+
Sbjct: 217 AAVPHLFLPLACAANVVKNVAAVT 240
Score = 37.5 bits (83), Expect = 0.017
Identities = 18/56 (32%), Positives = 30/56 (53%)
Query: 170 KKKYPKASHLKNIKDVYENRNYIIIPNVQDRKMYVLLNEKAMNDDILRAYFHAVLL 225
K + S +K ++ Y++ + K+Y LL +A +DDIL+A FHA +L
Sbjct: 365 KDAFQDPSTYMAVKPFFDKERYMVTYSPTKGKVYALLKHQANSDDILKAAFHAHVL 420
>At2g23470.1 68415.m02802 expressed protein contains Pfam domain,
PF04884: Protein of unknown function, DUF647
Length = 405
Score = 48.0 bits (109), Expect = 1e-05
Identities = 21/62 (33%), Positives = 32/62 (51%)
Query: 27 PDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVGDTTATPLAATVTWVIKDGCG 86
P VS +Y +Y W S+ LATQ + R +G+G + + +A W++KDG G
Sbjct: 125 PKQVSDNYISYVKWKFLHRVFSSALQVLATQAMFRAIGIGQSRSLASSAAFNWILKDGLG 184
Query: 87 HL 88
L
Sbjct: 185 RL 186
>At5g14220.1 68418.m01661 protoporphyrinogen oxidase, putative
similar to protoporphyrinogen IX oxidase, mitochondrial
(PPO II) from Nicotiana tabacum [SP|O24164], Glycine
max, AB025102, Spinacia oleracea [GI:14349153]; contains
Pfam amine oxidase, flavin-containing domain [PF015930]
Length = 508
Score = 33.5 bits (73), Expect = 0.27
Identities = 15/46 (32%), Positives = 26/46 (56%)
Query: 27 PDSVSRDYSAYQIWDTAQAFCSTITGTLATQEVLRGVGVGDTTATP 72
PDS+S +S +W+ ++F S I G + T+ +G DT ++P
Sbjct: 185 PDSLSMKHSFPDLWNVEKSFGSIIVGAIRTKFAAKGGKSRDTKSSP 230
>At4g13650.1 68417.m02123 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 1064
Score = 31.1 bits (67), Expect = 1.4
Identities = 32/110 (29%), Positives = 50/110 (45%), Gaps = 5/110 (4%)
Query: 73 LAATVTWVIKDGCGHLGRILFAFTHGTCLDAYSKKWRLYADILNDAAMC--IEIALPYFK 130
LA+ V DG G+ L A+T T L ++ ++ +LN A C IE AL YF
Sbjct: 392 LASLVVACSADGTLFRGQQLHAYT--TKL-GFASNNKIEGALLNLYAKCADIETALDYFL 448
Query: 131 NYTTVVLCVSTVMKAIVGVSDLKLCGFQIKMGVSIKDLIKKKYPKASHLK 180
+ + VM G+ D F+I + I++++ +Y S LK
Sbjct: 449 ETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPSILK 498
>At4g27500.1 68417.m03950 expressed protein non-consensus GA donor
splice site at exon 6
Length = 612
Score = 30.7 bits (66), Expect = 1.9
Identities = 17/44 (38%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Query: 378 KKEASKRRVHDIG--TETESPEIEEYREPNTEAEVKAEVPKENR 419
KKE K+ G TETE+ E+ E E EA V+ E P++ +
Sbjct: 514 KKEQEKKAKKKTGGNTETETEEVPEASEEEIEAPVQEEKPQKEK 557
>At3g02020.1 68416.m00164 aspartate kinase, lysine-sensitive,
putative similar to aspartate kinase gi:2257743
(Arabidopsis thaliana)
Length = 559
Score = 30.7 bits (66), Expect = 1.9
Identities = 20/55 (36%), Positives = 26/55 (47%)
Query: 339 KDYVISEADAKLTKELLMELEHGDGVTYGVVETKPLMHYKKEASKRRVHDIGTET 393
K V+ A AK T +LLM E V+T + Y KE R H++G ET
Sbjct: 114 KPVVVLSAMAKTTNKLLMAGEKAVCCGVTNVDTIEELSYIKELHIRTAHELGVET 168
>At1g52620.1 68414.m05941 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 819
Score = 30.3 bits (65), Expect = 2.5
Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 6/65 (9%)
Query: 94 AFTHGTCLDAYSKKWRLYADILNDAAMCIEIALPYF----KNYTTVVLC-VSTVMKAIVG 148
A HG C+ SK+WR D N +E+A+ Y ++ V+C ST++ A+V
Sbjct: 750 AILHGFCVVGNSKQWR-NMDFCNLGEKGLEVAVRYSQVLEQHLPQPVICEASTILHAMVE 808
Query: 149 VSDLK 153
+D K
Sbjct: 809 KADTK 813
>At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF05178:
Krr1 family
Length = 638
Score = 29.9 bits (64), Expect = 3.3
Identities = 28/106 (26%), Positives = 46/106 (43%), Gaps = 18/106 (16%)
Query: 312 LLMVDEWRISSVKPLSQYAPIEGRVSTKDYVISEADAKLTKELLMELEHGDGVTYGVVET 371
+L +D+ ++ PL + AP KD+ ++ K K + EL G
Sbjct: 522 ILTLDDAELNQYVPLKKMAPYV----EKDWEVNRHKVKEQKRKIRELWEGK--------- 568
Query: 372 KPLMHYKKEASKRRVHDIGTETESPEIEEYREPNTEAEVKAEVPKE 417
H +K++ KR+ +D+ ET+ E E EAE +AE E
Sbjct: 569 ----HDEKKSKKRKKNDV-VETKPTPKEAEAEAEAEAEAEAEAEAE 609
>At4g30960.1 68417.m04395 CBL-interacting protein kinase 6 (CIPK6)
identical to CBL-interacting protein kinase 6
[Arabidopsis thaliana] gi|9280634|gb|AAF86505
Length = 441
Score = 29.5 bits (63), Expect = 4.4
Identities = 21/93 (22%), Positives = 45/93 (48%), Gaps = 6/93 (6%)
Query: 140 STVMKAIVGVSDLKLCGF--QIKMGVSIKDLIKKKYPKASHLKNIKDVYENRNYIIIPNV 197
S MK +VG + G QIK +S+ ++K +P L + +++ Y + V
Sbjct: 49 SVAMK-VVGKEKVVKVGMVDQIKREISVMRMVK--HPNIVELHEVM-ASKSKIYFAMELV 104
Query: 198 QDRKMYVLLNEKAMNDDILRAYFHAVLLSVIIC 230
+ +++ + + + +D+ R YF ++ +V C
Sbjct: 105 RGGELFAKVAKGRLREDVARVYFQQLISAVDFC 137
>At3g23000.1 68416.m02900 CBL-interacting protein kinase 7 (CIPK7)
identical to CBL-interacting protein kinase 7
[Arabidopsis thaliana] gi|13249113|gb|AAK16682; contains
Pfam profiles PF00069: Protein kinase domain and
PF03822: NAF domain; identical to cDNA CBL-interacting
protein kinase 7 (CIPK7) GI:13249112
Length = 429
Score = 29.5 bits (63), Expect = 4.4
Identities = 13/63 (20%), Positives = 29/63 (46%)
Query: 239 VYQAPNEERPFAQVCRTIQTTDWSREPIETHVFNEFKFEPSYDLMKYVNRIVQREWMRIK 298
+YQ + T+ T+W ++ +ET F+ F+ ++ VN I + + +
Sbjct: 257 IYQMLDPNPVTRMSIETVMKTNWFKKSLETSEFHRNVFDSEVEMKSSVNSITAFDLISLS 316
Query: 299 TGM 301
+G+
Sbjct: 317 SGL 319
>At2g32700.5 68415.m04001 WD-40 repeat family protein contains 7
WD-40 repeats ; similar to LEUNIG
(GP:11141605)[Arabidopsis thaliana]
Length = 785
Score = 29.5 bits (63), Expect = 4.4
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Query: 15 NWFTNVFLPKGYPDS-VSRDYSAYQIWDTAQAFCSTITG 52
N F +V YPD V Y A ++W+T + C T+ G
Sbjct: 717 NKFHSVVFHPSYPDLLVIGGYQAIELWNTMENKCMTVAG 755
>At2g32700.4 68415.m04000 WD-40 repeat family protein contains 7
WD-40 repeats ; similar to LEUNIG
(GP:11141605)[Arabidopsis thaliana]
Length = 787
Score = 29.5 bits (63), Expect = 4.4
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Query: 15 NWFTNVFLPKGYPDS-VSRDYSAYQIWDTAQAFCSTITG 52
N F +V YPD V Y A ++W+T + C T+ G
Sbjct: 719 NKFHSVVFHPSYPDLLVIGGYQAIELWNTMENKCMTVAG 757
>At2g32700.3 68415.m03999 WD-40 repeat family protein contains 7
WD-40 repeats ; similar to LEUNIG
(GP:11141605)[Arabidopsis thaliana]
Length = 787
Score = 29.5 bits (63), Expect = 4.4
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Query: 15 NWFTNVFLPKGYPDS-VSRDYSAYQIWDTAQAFCSTITG 52
N F +V YPD V Y A ++W+T + C T+ G
Sbjct: 719 NKFHSVVFHPSYPDLLVIGGYQAIELWNTMENKCMTVAG 757
>At2g32700.2 68415.m03998 WD-40 repeat family protein contains 7
WD-40 repeats ; similar to LEUNIG
(GP:11141605)[Arabidopsis thaliana]
Length = 787
Score = 29.5 bits (63), Expect = 4.4
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Query: 15 NWFTNVFLPKGYPDS-VSRDYSAYQIWDTAQAFCSTITG 52
N F +V YPD V Y A ++W+T + C T+ G
Sbjct: 719 NKFHSVVFHPSYPDLLVIGGYQAIELWNTMENKCMTVAG 757
>At2g32700.1 68415.m03997 WD-40 repeat family protein contains 7
WD-40 repeats ; similar to LEUNIG
(GP:11141605)[Arabidopsis thaliana]
Length = 787
Score = 29.5 bits (63), Expect = 4.4
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Query: 15 NWFTNVFLPKGYPDS-VSRDYSAYQIWDTAQAFCSTITG 52
N F +V YPD V Y A ++W+T + C T+ G
Sbjct: 719 NKFHSVVFHPSYPDLLVIGGYQAIELWNTMENKCMTVAG 757
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 29.5 bits (63), Expect = 4.4
Identities = 19/83 (22%), Positives = 41/83 (49%), Gaps = 3/83 (3%)
Query: 340 DYVISEADAKLTKELLMELEHGDGVTYGVVETKPLMHYKKEASKRRVHDIGTETESPEIE 399
D + E+D K+ + E EH G E + KK+ +K+ + G E ++ + +
Sbjct: 98 DLEVKESDVKVEEH---EKEHKKGKEKKHEELEEEKEGKKKKNKKEKDESGPEEKNKKAD 154
Query: 400 EYREPNTEAEVKAEVPKENRKKD 422
+ ++ ++ K E+ +E+ KK+
Sbjct: 155 KEKKHEDVSQEKEELEEEDGKKN 177
>At5g21222.1 68418.m02532 protein kinase family protein contains
Pfam profile: PF00069 protein kinase domain
Length = 831
Score = 28.7 bits (61), Expect = 7.7
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Query: 158 QIKMGVSIKDLIKKKYPKASHLKNIKDVYENRNYIIIPNVQDRKMY-VLLNEKAMNDDIL 216
QIK +SI LI +P L + + + YI++ + K++ + N+ MN+D
Sbjct: 57 QIKREISIMKLIN--HPNVVQLYEVL-ASKAKIYIVLEFISGGKLFDKIKNDGRMNEDEA 113
Query: 217 RAYFHAVLLSVIIC 230
+ YF ++ +V C
Sbjct: 114 QRYFQQLINAVDYC 127
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.136 0.411
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,170,079
Number of Sequences: 28952
Number of extensions: 429389
Number of successful extensions: 1114
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1095
Number of HSP's gapped (non-prelim): 26
length of query: 422
length of database: 12,070,560
effective HSP length: 83
effective length of query: 339
effective length of database: 9,667,544
effective search space: 3277297416
effective search space used: 3277297416
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 61 (28.7 bits)
- SilkBase 1999-2023 -