Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000544-TA|BGIBMGA000544-PA|IPR001611|Leucine-rich
repeat, IPR003591|Leucine-rich repeat, typical subtype
         (562 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF322227-1|AAK01654.1|  782|Tribolium castaneum cell surface pro...    98   2e-22
AM292380-1|CAL23192.2|  489|Tribolium castaneum gustatory recept...    23   7.4  
AM292346-1|CAL23158.2|  314|Tribolium castaneum gustatory recept...    23   7.4  

>AF322227-1|AAK01654.1|  782|Tribolium castaneum cell surface
           protein chaoptin protein.
          Length = 782

 Score = 97.9 bits (233), Expect = 2e-22
 Identities = 80/280 (28%), Positives = 138/280 (49%), Gaps = 21/280 (7%)

Query: 5   LKESVFTKLDLVDYFSLADNSLPELPRHVLQHLPHVKTLDLCRNKITKLTEEDFRDIQEL 64
           +++  F  L  ++   L DN +  L R    +L  +K L+L  NKI  +  E F+++ EL
Sbjct: 107 VQQHTFADLIQLEQIHLDDNRIESLERRAFMNLKSLKRLNLKGNKIATIAYETFQNLPEL 166

Query: 65  EHLLVADNQISEIEKDALPK----GLKHVHLGINKLNTL----------NGALRDLDDLE 110
           E L +A N IS ++ +   +    G+ HV++  NKL  L          +  L  L +++
Sbjct: 167 EDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQNIK 226

Query: 111 WIFINANNLKSIENEL--PTKAKKMQLIHAAHNELQSLPSDL-KLMPSLQSLYFYGNNIK 167
            + ++ NN+ S+  +   P +   MQL +  HN+L +   DL   MP LQ L    N++ 
Sbjct: 227 VLDLSFNNITSVAKQFFRPVELSLMQL-YLGHNKLLNATKDLFGNMPHLQVLDLSHNSLY 285

Query: 168 SLD-ETLQKSRNLMRIGLSFNKIEFLTKDDFTEAEKLAELDIAYNELKYLNGSLRSLKSL 226
            LD +T + ++ L  +  S N+I  +  D F     L  +D ++N L+ L  +L     L
Sbjct: 286 ELDFDTFRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLFRETGL 345

Query: 227 RYLNLTHNYMTEFSLQEIK--GLRRLSVIDLSHNQISLLT 264
             L+++HN + +  L  +     + LS +DLS N IS L+
Sbjct: 346 ERLDVSHNLLGKLPLTSLSLASAQTLSELDLSWNSISSLS 385



 Score = 92.3 bits (219), Expect = 8e-21
 Identities = 76/296 (25%), Positives = 149/296 (50%), Gaps = 22/296 (7%)

Query: 10  FTKLDLVDYFSLADNSLPELPRHVLQHLPHVKT--LDLCRNKITKLT-------EED--F 58
           F  L  ++   LA NS+  L  ++   +  +    +++  NK+  L        E+D   
Sbjct: 160 FQNLPELEDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGL 219

Query: 59  RDIQELEHLLVADNQISEIEKD---ALPKGLKHVHLGINKL-NTLNGALRDLDDLEWIFI 114
             +Q ++ L ++ N I+ + K     +   L  ++LG NKL N       ++  L+ + +
Sbjct: 220 GGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDLFGNMPHLQVLDL 279

Query: 115 NANNLKSIENELPTKAKKMQLIHAAHNELQSLPSDL-KLMPSLQSLYFYGNNIKSLDETL 173
           + N+L  ++ +     KK+Q +  +HN +  +P+DL + + +L+ + F  N ++SL + L
Sbjct: 280 SHNSLYELDFDTFRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNL 339

Query: 174 QKSRNLMRIGLSFNKIEFL--TKDDFTEAEKLAELDIAYNELKYLN--GSLRSLKSLRYL 229
            +   L R+ +S N +  L  T      A+ L+ELD+++N +  L+  G L   K L +L
Sbjct: 340 FRETGLERLDVSHNLLGKLPLTSLSLASAQTLSELDLSWNSISSLSHGGQLARFKCLSWL 399

Query: 230 NLTHNYMTEFSLQEIKGLRRLSVIDLSHN-QISLLTGSLENLVDVETRVLELRLDH 284
           +L++N + +      KG+ RL+ ++L HN Q++L    L +   +E  +L L LD+
Sbjct: 400 DLSYNRLGQIDAGTFKGIPRLASLNLGHNSQLTLEINGL-SFQGLEYTLLHLNLDN 454



 Score = 65.3 bits (152), Expect = 1e-12
 Identities = 57/257 (22%), Positives = 113/257 (43%), Gaps = 20/257 (7%)

Query: 24  NSLPELPRHVLQHLPHVKTLDLCRNKITKLTEEDFRDIQELEHLLVADNQISEIEKDA-- 81
           +S+   P   ++ L  ++ LDL  N++  + +  F  ++ L+ + + DN I  I +    
Sbjct: 28  SSVQNFPSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNTIEMIHRGTFQ 87

Query: 82  --LPKGLKHVHLGINKL-NTLNGALRDLDDLEWIFINANNLKSIENELPTKAKKMQLIHA 138
             + + L  V+   N + N       DL  LE I ++ N ++S+E       K ++ ++ 
Sbjct: 88  GDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAFMNLKSLKRLNL 147

Query: 139 AHNELQSLPSD-LKLMPSLQSLYFYGNNIKSLDETL---QKSRNLMRIGLSFNK------ 188
             N++ ++  +  + +P L+ L    N+I SLD  +     S  +  + +S NK      
Sbjct: 148 KGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVV 207

Query: 189 ---IEFLTKDDFTEAEKLAELDIAYNEL-KYLNGSLRSLK-SLRYLNLTHNYMTEFSLQE 243
              + F         + +  LD+++N +        R ++ SL  L L HN +   +   
Sbjct: 208 APSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDL 267

Query: 244 IKGLRRLSVIDLSHNQI 260
              +  L V+DLSHN +
Sbjct: 268 FGNMPHLQVLDLSHNSL 284



 Score = 63.7 bits (148), Expect = 3e-12
 Identities = 56/263 (21%), Positives = 117/263 (44%), Gaps = 10/263 (3%)

Query: 10  FTKLDLVDYFSLADNSLPELPRHVLQHLPHVKTLDLCRNKITKLTEEDFRDIQELEHLLV 69
           F     + +   + N + E+P  + + L +++ +D   N++  L +  FR+   LE L V
Sbjct: 292 FRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLFRETG-LERLDV 350

Query: 70  ADNQISEIEKDALP----KGLKHVHLGINKLNTLN--GALRDLDDLEWIFINANNLKSIE 123
           + N + ++   +L     + L  + L  N +++L+  G L     L W+ ++ N L  I+
Sbjct: 351 SHNLLGKLPLTSLSLASAQTLSELDLSWNSISSLSHGGQLARFKCLSWLDLSYNRLGQID 410

Query: 124 NELPTKAKKMQLIHAAHNELQSLPSDLKLMPSLQSLYFYGN--NIKSLDETLQKSRNLMR 181
                   ++  ++  HN   +L  +      L+    + N  N+         + NL+ 
Sbjct: 411 AGTFKGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLLHLNLDNVSLSQVPALSTPNLLS 470

Query: 182 IGLSFNKIEFLTKDDFTEAEKLAELDIAYNELKYLNGSLRSLKSLRYLNLTHNYMTEFSL 241
           + L+FN +  +  +       L  L++ YN+L  +     SL  LR+L+L  N +T  S 
Sbjct: 471 LSLAFNSLPTVALEVAGNISSLRYLNLDYNDLSAVPIVTHSLTELRHLSLEGNPITTLSN 530

Query: 242 QEIKG-LRRLSVIDLSHNQISLL 263
             + G   +L  ++L +  +++L
Sbjct: 531 TSLLGAANQLEELNLKNIDLTVL 553



 Score = 50.8 bits (116), Expect = 2e-08
 Identities = 59/272 (21%), Positives = 116/272 (42%), Gaps = 18/272 (6%)

Query: 133 MQLIHAAHNELQSLPSD-LKLMPSLQSLYFYGNNIKSL-DETLQKSRNLMRIGLSFNKIE 190
           ++L HA  + +Q+ PSD +K++  L+ L    N ++++ D +    R+L ++ L  N IE
Sbjct: 20  LKLTHALSSSVQNFPSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNTIE 79

Query: 191 FLTKDDFTE--AEKLAELDIAYNELKYL-NGSLRSLKSLRYLNLTHNYMTEFSLQEIKGL 247
            + +  F       L E+  ++N ++ +   +   L  L  ++L  N +     +    L
Sbjct: 80  MIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAFMNL 139

Query: 248 RRLSVIDLSHNQISLLT-GSLENLVDVETRVLELR----LDHNHIFXXXXXXXXXXXXXX 302
           + L  ++L  N+I+ +   + +NL ++E   L       LD N IF              
Sbjct: 140 KSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFN-IFDQVGSLGMFHVNMS 198

Query: 303 XXXXXXXIQKIXXXXXXXXXXXXXXXV-----SYNHITTL-EETSKTFLPSLEELIAHHN 356
                  +                  +     S+N+IT++ ++  +    SL +L   HN
Sbjct: 199 HNKLINLVVAPSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHN 258

Query: 357 NITVLDKDFHG-LPSLCSADLSFNKIHTVNYD 387
            +    KD  G +P L   DLS N ++ +++D
Sbjct: 259 KLLNATKDLFGNMPHLQVLDLSHNSLYELDFD 290



 Score = 36.3 bits (80), Expect = 6e-04
 Identities = 40/172 (23%), Positives = 69/172 (40%), Gaps = 12/172 (6%)

Query: 225 SLRYLNLTH---NYMTEFSLQEIKGLRRLSVIDLSHNQISLLTGS----LENLVDVETRV 277
           SL  L LTH   + +  F    IK L RL  +DLS+N++  +  +    L +L  V  + 
Sbjct: 16  SLLTLKLTHALSSSVQNFPSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQD 75

Query: 278 LELRLDHNHIFXXXXXXXXXXXXXXXXXXXXXIQKIXXXXXXXXXXXXXXXVSYNHITTL 337
             + + H   F                     ++ +               +  N I +L
Sbjct: 76  NTIEMIHRGTFQGDIHRDLTEVYFSFNS----VRNVQQHTFADLIQLEQIHLDDNRIESL 131

Query: 338 EETSKTFLPSLEELIAHHNNI-TVLDKDFHGLPSLCSADLSFNKIHTVNYDL 388
           E  +   L SL+ L    N I T+  + F  LP L   DL++N I ++++++
Sbjct: 132 ERRAFMNLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNI 183


>AM292380-1|CAL23192.2|  489|Tribolium castaneum gustatory receptor
           candidate 59 protein.
          Length = 489

 Score = 22.6 bits (46), Expect = 7.4
 Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 3/48 (6%)

Query: 389 VSKSRCTINGVPSILKIYLQDNPVLCDDHLYELVTVFESL---NARVS 433
           VSK+R  +  +P  + +Y++      +D   +L   F S    NAR+S
Sbjct: 387 VSKARQFLPALPGYVPVYIRPGDTPLEDINPDLAEAFSSYARKNARLS 434


>AM292346-1|CAL23158.2|  314|Tribolium castaneum gustatory receptor
           candidate 25 protein.
          Length = 314

 Score = 22.6 bits (46), Expect = 7.4
 Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 3/48 (6%)

Query: 389 VSKSRCTINGVPSILKIYLQDNPVLCDDHLYELVTVFESL---NARVS 433
           VSK+R  +  +P  + +Y++      +D   +L   F S    NAR+S
Sbjct: 212 VSKARQFLPALPGYVPVYIRPGDTPLEDINPDLAEAFSSYARKNARLS 259


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.318    0.135    0.387 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 121,525
Number of Sequences: 317
Number of extensions: 5230
Number of successful extensions: 33
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 5
Number of HSP's gapped (non-prelim): 11
length of query: 562
length of database: 114,650
effective HSP length: 60
effective length of query: 502
effective length of database: 95,630
effective search space: 48006260
effective search space used: 48006260
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 45 (22.2 bits)