SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000535-TA|BGIBMGA000535-PA|IPR008937|Ras guanine
nucleotide exchange factor, IPR001895|Guanine-nucleotide dissociation
stimulator CDC25
         (433 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ...    57   6e-09
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    54   3e-08
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy...    32   0.14 
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo...    31   0.42 
SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces pombe...    31   0.42 
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    29   1.7  
SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4 |S...    28   2.2  
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch...    28   2.9  
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc...    27   3.9  
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces...    27   5.1  
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    27   5.1  
SPBC1703.03c |||armadillo repeat protein, unknown biological rol...    27   5.1  
SPBC725.09c |hob3||BAR adaptor protein Hob3|Schizosaccharomyces ...    27   6.8  
SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch...    27   6.8  
SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo...    26   8.9  

>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
           Ste6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 911

 Score = 56.8 bits (131), Expect = 6e-09
 Identities = 54/200 (27%), Positives = 85/200 (42%), Gaps = 20/200 (10%)

Query: 162 KLTALEQYEESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVELHRLSFVGPEEFV 221
           K+   ++  + L D++  +S D +   D L L L P E+A QL I+E    S +   +F+
Sbjct: 629 KMLEGKELSQELEDLSLHNSPDPIIYKDELVLLLPPREIAKQLCILEFQSFSHISRIQFL 688

Query: 222 QTFAPIHPPQSTSACKTSISLHHIDTKCTRNLEAYADWFNRLSYLVATDILKGVKSKYRA 281
                I    +  + K   S  ++               N L   V   I++  + + R 
Sbjct: 689 ---TKIWDNLNRFSPKEKTSTFYLS--------------NHLVNFVTETIVQEEEPRRRT 731

Query: 282 RVIEQWVMTGRECFNLGNFNSLMAIISALNMSPVMRLKKTWSRTSAVCGQQLRQLELCVE 341
            V+  ++        L NF SL +IISALN SP+ RL+KTW+  ++   + L   EL   
Sbjct: 732 NVLAYFIQVCDYLRELNNFASLFSIISALNSSPIHRLRKTWANLNS---KTLASFELLNN 788

Query: 342 PTGNHARLSGNRVNFEKCAL 361
            T      S  R   E C L
Sbjct: 789 LTEARKNFSNYRDCLENCVL 808


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 54.4 bits (125), Expect = 3e-08
 Identities = 28/84 (33%), Positives = 48/84 (57%), Gaps = 3/84 (3%)

Query: 256 YADWFNRLSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMSPV 315
           Y ++ N  +Y +   IL+   +K R  VI  ++ T  +C +L NF++LM+I+SALN +P+
Sbjct: 793 YINFSNCFTYWIINCILEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPI 852

Query: 316 MRLKKTWSRTSA---VCGQQLRQL 336
            RL   +    A   +C   LR++
Sbjct: 853 YRLHAAYKLVKAEDIICLSGLREI 876


>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 4924

 Score = 32.3 bits (70), Expect = 0.14
 Identities = 38/132 (28%), Positives = 58/132 (43%), Gaps = 14/132 (10%)

Query: 202  NQLTIVELHRLSFVG--PEEFVQTFAPIHPPQSTSACKTSISLHHIDTKC-----TRNLE 254
            N+   +E+    F+G  P +F +    I+P Q T  C  SISL  ID  C     T +  
Sbjct: 1491 NKNVDLEIWSKIFLGFKPSKFPELCKDINPEQRTYKCNLSISLSQIDDLCNGFSFTTSTF 1550

Query: 255  AYADWFNRLSYLV-ATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMS 313
                W   LS+L+ + DI  G     R   +++ V      FN  +F   + + S L+ +
Sbjct: 1551 LQCCWAKTLSFLLNSRDICFGNIISGRDVPVDEVVSLVAPTFN--SFPLRVLLDSKLSFA 1608

Query: 314  PV----MRLKKT 321
             V     RLK+T
Sbjct: 1609 EVCGQLQRLKET 1620


>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
           Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 778

 Score = 30.7 bits (66), Expect = 0.42
 Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 7/53 (13%)

Query: 39  SLTSDPTLLHGTGLSYYDLSTVLTPGVKA-IPNHPYTNCTTEKSDIEYRDGQI 90
           +L+ +P   + T L+YYD+ T+   G  A + +HP      EK  I Y DG+I
Sbjct: 326 TLSDNPITANSTILNYYDIVTIKHMGTNAFLHSHP------EKYPIPYDDGRI 372


>SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 590

 Score = 30.7 bits (66), Expect = 0.42
 Identities = 23/92 (25%), Positives = 45/92 (48%), Gaps = 6/92 (6%)

Query: 94  EVKDLVEKELNDMIALVRVLRQWTSMFPYDFRDDRVMALVRSITQRCAAEHMESVRVEMS 153
           EV  + ++  NDM+  V+ L   + +  Y  +D+++  L   I  RC +E M S+ +   
Sbjct: 202 EVNQVTQQHPNDMVLCVQWLANVSLLLFYLKKDNKLDDLTVDIQNRC-SELMNSLYI--- 257

Query: 154 TTLEKLLDKLTALEQYEESLVDITQVSSLDQL 185
           T  +  + ++   E  EE +V  T +  L+ +
Sbjct: 258 TICQDAMRRMN--ENLEEGMVKYTGIQGLEDI 287


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 11/29 (37%), Positives = 19/29 (65%)

Query: 89  QIGKAEVKDLVEKELNDMIALVRVLRQWT 117
           + GK+EV+DL++  LN+ +  +   R WT
Sbjct: 580 KFGKSEVRDLLKSSLNEKVMDLLAERYWT 608


>SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 28.3 bits (60), Expect = 2.2
 Identities = 13/33 (39%), Positives = 17/33 (51%)

Query: 227 IHPPQSTSACKTSISLHHIDTKCTRNLEAYADW 259
           +  P S S+ K   +LHH   K   + EAY DW
Sbjct: 70  VRQPFSISSRKDDFTLHHWVLKSEVDSEAYEDW 102


>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 27.9 bits (59), Expect = 2.9
 Identities = 20/59 (33%), Positives = 28/59 (47%), Gaps = 2/59 (3%)

Query: 165 ALEQYEESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVELHRLSFVGPEEFVQT 223
           A E Y  +  D    SS+ Q S  ++ T   TP E A+++  V L+   F G  E V T
Sbjct: 479 AYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPAEPASKVDGVVLY--DFTGEHEGVIT 535


>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 354

 Score = 27.5 bits (58), Expect = 3.9
 Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 1/50 (2%)

Query: 93  AEVKDLVEKELNDMIALVRVLRQWTSMFPYDFRDDRVMALVRSITQRCAA 142
           A V D +EKE   +I   RVL+   S+  Y  +D   + LV+++ Q  AA
Sbjct: 34  APVAD-IEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLVKTLGQNPAA 82


>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1727

 Score = 27.1 bits (57), Expect = 5.1
 Identities = 29/94 (30%), Positives = 48/94 (51%), Gaps = 8/94 (8%)

Query: 93   AEVKDLVEKELNDMIALVRVLRQWTSMFPYDFRD-DRVMA---LVRSITQRCAAEHMESV 148
            +E + L+E  L+D+ +    L    S + Y  R+ +++ A   L   + +R   E+ E  
Sbjct: 911  SEREALLESSLSDLQSKHTSLE---SQYNYSLRNIEQLQAASKLAEEMVERVKTEYDE-Y 966

Query: 149  RVEMSTTLEKLLDKLTALEQYEESLVDITQVSSL 182
            R++ S +LEK   K+T+LEQ    L D    SSL
Sbjct: 967  RLQTSESLEKNHLKITSLEQRIVILQDEIASSSL 1000


>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 570

 Score = 27.1 bits (57), Expect = 5.1
 Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 7/61 (11%)

Query: 46  LLHGTGLSYYDLSTV-----LTPGVKAIPNHPYTNCTTEKSDIE-YRDGQIGKAEVKDLV 99
           L+HG    Y D++T      L  G  A  +  Y N  +EK  ++ ++D + GKA+V  LV
Sbjct: 486 LIHGLHAVYNDINTEWDYQNLLKGYGAKNSRSY-NIHSEKELLDLFKDEEFGKADVIQLV 544

Query: 100 E 100
           E
Sbjct: 545 E 545


>SPBC1703.03c |||armadillo repeat protein, unknown biological
           role|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 664

 Score = 27.1 bits (57), Expect = 5.1
 Identities = 12/35 (34%), Positives = 22/35 (62%)

Query: 152 MSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS 186
           M++ L+ L D    LE+ E+SL++   +S +D +S
Sbjct: 346 MTSLLQSLADGTDELEEQEDSLMEDEDLSYMDDMS 380


>SPBC725.09c |hob3||BAR adaptor protein Hob3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 264

 Score = 26.6 bits (56), Expect = 6.8
 Identities = 17/58 (29%), Positives = 27/58 (46%)

Query: 58  STVLTPGVKAIPNHPYTNCTTEKSDIEYRDGQIGKAEVKDLVEKELNDMIALVRVLRQ 115
           +TVL P  +     P  N    K + +  D    +A+V+ LV+K  ND   L R  ++
Sbjct: 112 TTVLDPISRFCSYFPDINAAITKRNHKLLDHDAMRAKVQKLVDKPSNDTTKLPRTEKE 169


>SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 747

 Score = 26.6 bits (56), Expect = 6.8
 Identities = 15/50 (30%), Positives = 24/50 (48%)

Query: 219 EFVQTFAPIHPPQSTSACKTSISLHHIDTKCTRNLEAYADWFNRLSYLVA 268
           +F+QT   I    S++  +T +SL+ +  +C   L   A  FN    L A
Sbjct: 309 KFLQTLETILKSFSSTLDETQLSLYQVAVRCCTKLIEQASHFNLSEKLFA 358


>SPAC3C7.12 |tip1|noc1|CLIP170 family protein
           Tip1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 461

 Score = 26.2 bits (55), Expect = 8.9
 Identities = 15/40 (37%), Positives = 20/40 (50%)

Query: 170 EESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVEL 209
           + S+  +T VSS   LS   I +  LTP E   Q  I +L
Sbjct: 106 QSSIPRLTNVSSSSNLSMNTISSTALTPTEKILQKRIEDL 145


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.131    0.383 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,776,501
Number of Sequences: 5004
Number of extensions: 65513
Number of successful extensions: 176
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 164
Number of HSP's gapped (non-prelim): 17
length of query: 433
length of database: 2,362,478
effective HSP length: 75
effective length of query: 358
effective length of database: 1,987,178
effective search space: 711409724
effective search space used: 711409724
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 55 (26.2 bits)

- SilkBase 1999-2023 -