BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000535-TA|BGIBMGA000535-PA|IPR008937|Ras guanine nucleotide exchange factor, IPR001895|Guanine-nucleotide dissociation stimulator CDC25 (433 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 57 6e-09 SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 54 3e-08 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 32 0.14 SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 31 0.42 SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces pombe... 31 0.42 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 29 1.7 SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4 |S... 28 2.2 SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 28 2.9 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 27 3.9 SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 27 5.1 SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 27 5.1 SPBC1703.03c |||armadillo repeat protein, unknown biological rol... 27 5.1 SPBC725.09c |hob3||BAR adaptor protein Hob3|Schizosaccharomyces ... 27 6.8 SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosacch... 27 6.8 SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharo... 26 8.9 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 56.8 bits (131), Expect = 6e-09 Identities = 54/200 (27%), Positives = 85/200 (42%), Gaps = 20/200 (10%) Query: 162 KLTALEQYEESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVELHRLSFVGPEEFV 221 K+ ++ + L D++ +S D + D L L L P E+A QL I+E S + +F+ Sbjct: 629 KMLEGKELSQELEDLSLHNSPDPIIYKDELVLLLPPREIAKQLCILEFQSFSHISRIQFL 688 Query: 222 QTFAPIHPPQSTSACKTSISLHHIDTKCTRNLEAYADWFNRLSYLVATDILKGVKSKYRA 281 I + + K S ++ N L V I++ + + R Sbjct: 689 ---TKIWDNLNRFSPKEKTSTFYLS--------------NHLVNFVTETIVQEEEPRRRT 731 Query: 282 RVIEQWVMTGRECFNLGNFNSLMAIISALNMSPVMRLKKTWSRTSAVCGQQLRQLELCVE 341 V+ ++ L NF SL +IISALN SP+ RL+KTW+ ++ + L EL Sbjct: 732 NVLAYFIQVCDYLRELNNFASLFSIISALNSSPIHRLRKTWANLNS---KTLASFELLNN 788 Query: 342 PTGNHARLSGNRVNFEKCAL 361 T S R E C L Sbjct: 789 LTEARKNFSNYRDCLENCVL 808 >SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 987 Score = 54.4 bits (125), Expect = 3e-08 Identities = 28/84 (33%), Positives = 48/84 (57%), Gaps = 3/84 (3%) Query: 256 YADWFNRLSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMSPV 315 Y ++ N +Y + IL+ +K R VI ++ T +C +L NF++LM+I+SALN +P+ Sbjct: 793 YINFSNCFTYWIINCILEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPI 852 Query: 316 MRLKKTWSRTSA---VCGQQLRQL 336 RL + A +C LR++ Sbjct: 853 YRLHAAYKLVKAEDIICLSGLREI 876 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 32.3 bits (70), Expect = 0.14 Identities = 38/132 (28%), Positives = 58/132 (43%), Gaps = 14/132 (10%) Query: 202 NQLTIVELHRLSFVG--PEEFVQTFAPIHPPQSTSACKTSISLHHIDTKC-----TRNLE 254 N+ +E+ F+G P +F + I+P Q T C SISL ID C T + Sbjct: 1491 NKNVDLEIWSKIFLGFKPSKFPELCKDINPEQRTYKCNLSISLSQIDDLCNGFSFTTSTF 1550 Query: 255 AYADWFNRLSYLV-ATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMS 313 W LS+L+ + DI G R +++ V FN +F + + S L+ + Sbjct: 1551 LQCCWAKTLSFLLNSRDICFGNIISGRDVPVDEVVSLVAPTFN--SFPLRVLLDSKLSFA 1608 Query: 314 PV----MRLKKT 321 V RLK+T Sbjct: 1609 EVCGQLQRLKET 1620 >SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizosaccharomyces pombe|chr 2|||Manual Length = 778 Score = 30.7 bits (66), Expect = 0.42 Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 7/53 (13%) Query: 39 SLTSDPTLLHGTGLSYYDLSTVLTPGVKA-IPNHPYTNCTTEKSDIEYRDGQI 90 +L+ +P + T L+YYD+ T+ G A + +HP EK I Y DG+I Sbjct: 326 TLSDNPITANSTILNYYDIVTIKHMGTNAFLHSHP------EKYPIPYDDGRI 372 >SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces pombe|chr 1|||Manual Length = 590 Score = 30.7 bits (66), Expect = 0.42 Identities = 23/92 (25%), Positives = 45/92 (48%), Gaps = 6/92 (6%) Query: 94 EVKDLVEKELNDMIALVRVLRQWTSMFPYDFRDDRVMALVRSITQRCAAEHMESVRVEMS 153 EV + ++ NDM+ V+ L + + Y +D+++ L I RC +E M S+ + Sbjct: 202 EVNQVTQQHPNDMVLCVQWLANVSLLLFYLKKDNKLDDLTVDIQNRC-SELMNSLYI--- 257 Query: 154 TTLEKLLDKLTALEQYEESLVDITQVSSLDQL 185 T + + ++ E EE +V T + L+ + Sbjct: 258 TICQDAMRRMN--ENLEEGMVKYTGIQGLEDI 287 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 28.7 bits (61), Expect = 1.7 Identities = 11/29 (37%), Positives = 19/29 (65%) Query: 89 QIGKAEVKDLVEKELNDMIALVRVLRQWT 117 + GK+EV+DL++ LN+ + + R WT Sbjct: 580 KFGKSEVRDLLKSSLNEKVMDLLAERYWT 608 >SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 28.3 bits (60), Expect = 2.2 Identities = 13/33 (39%), Positives = 17/33 (51%) Query: 227 IHPPQSTSACKTSISLHHIDTKCTRNLEAYADW 259 + P S S+ K +LHH K + EAY DW Sbjct: 70 VRQPFSISSRKDDFTLHHWVLKSEVDSEAYEDW 102 >SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 27.9 bits (59), Expect = 2.9 Identities = 20/59 (33%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Query: 165 ALEQYEESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVELHRLSFVGPEEFVQT 223 A E Y + D SS+ Q S ++ T TP E A+++ V L+ F G E V T Sbjct: 479 AYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPAEPASKVDGVVLY--DFTGEHEGVIT 535 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 27.5 bits (58), Expect = 3.9 Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Query: 93 AEVKDLVEKELNDMIALVRVLRQWTSMFPYDFRDDRVMALVRSITQRCAA 142 A V D +EKE +I RVL+ S+ Y +D + LV+++ Q AA Sbjct: 34 APVAD-IEKERQRLIYAGRVLKDEESLKTYKIQDGHSIHLVKTLGQNPAA 82 >SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1727 Score = 27.1 bits (57), Expect = 5.1 Identities = 29/94 (30%), Positives = 48/94 (51%), Gaps = 8/94 (8%) Query: 93 AEVKDLVEKELNDMIALVRVLRQWTSMFPYDFRD-DRVMA---LVRSITQRCAAEHMESV 148 +E + L+E L+D+ + L S + Y R+ +++ A L + +R E+ E Sbjct: 911 SEREALLESSLSDLQSKHTSLE---SQYNYSLRNIEQLQAASKLAEEMVERVKTEYDE-Y 966 Query: 149 RVEMSTTLEKLLDKLTALEQYEESLVDITQVSSL 182 R++ S +LEK K+T+LEQ L D SSL Sbjct: 967 RLQTSESLEKNHLKITSLEQRIVILQDEIASSSL 1000 >SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 27.1 bits (57), Expect = 5.1 Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 7/61 (11%) Query: 46 LLHGTGLSYYDLSTV-----LTPGVKAIPNHPYTNCTTEKSDIE-YRDGQIGKAEVKDLV 99 L+HG Y D++T L G A + Y N +EK ++ ++D + GKA+V LV Sbjct: 486 LIHGLHAVYNDINTEWDYQNLLKGYGAKNSRSY-NIHSEKELLDLFKDEEFGKADVIQLV 544 Query: 100 E 100 E Sbjct: 545 E 545 >SPBC1703.03c |||armadillo repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 664 Score = 27.1 bits (57), Expect = 5.1 Identities = 12/35 (34%), Positives = 22/35 (62%) Query: 152 MSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS 186 M++ L+ L D LE+ E+SL++ +S +D +S Sbjct: 346 MTSLLQSLADGTDELEEQEDSLMEDEDLSYMDDMS 380 >SPBC725.09c |hob3||BAR adaptor protein Hob3|Schizosaccharomyces pombe|chr 2|||Manual Length = 264 Score = 26.6 bits (56), Expect = 6.8 Identities = 17/58 (29%), Positives = 27/58 (46%) Query: 58 STVLTPGVKAIPNHPYTNCTTEKSDIEYRDGQIGKAEVKDLVEKELNDMIALVRVLRQ 115 +TVL P + P N K + + D +A+V+ LV+K ND L R ++ Sbjct: 112 TTVLDPISRFCSYFPDINAAITKRNHKLLDHDAMRAKVQKLVDKPSNDTTKLPRTEKE 169 >SPBC887.03c |noc3||Noc2p-Noc3p complex subunit Noc3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 747 Score = 26.6 bits (56), Expect = 6.8 Identities = 15/50 (30%), Positives = 24/50 (48%) Query: 219 EFVQTFAPIHPPQSTSACKTSISLHHIDTKCTRNLEAYADWFNRLSYLVA 268 +F+QT I S++ +T +SL+ + +C L A FN L A Sbjct: 309 KFLQTLETILKSFSSTLDETQLSLYQVAVRCCTKLIEQASHFNLSEKLFA 358 >SPAC3C7.12 |tip1|noc1|CLIP170 family protein Tip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 461 Score = 26.2 bits (55), Expect = 8.9 Identities = 15/40 (37%), Positives = 20/40 (50%) Query: 170 EESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVEL 209 + S+ +T VSS LS I + LTP E Q I +L Sbjct: 106 QSSIPRLTNVSSSSNLSMNTISSTALTPTEKILQKRIEDL 145 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.131 0.383 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,776,501 Number of Sequences: 5004 Number of extensions: 65513 Number of successful extensions: 176 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 164 Number of HSP's gapped (non-prelim): 17 length of query: 433 length of database: 2,362,478 effective HSP length: 75 effective length of query: 358 effective length of database: 1,987,178 effective search space: 711409724 effective search space used: 711409724 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 55 (26.2 bits)
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