Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000535-TA|BGIBMGA000535-PA|IPR008937|Ras guanine
nucleotide exchange factor, IPR001895|Guanine-nucleotide dissociation
stimulator CDC25
(433 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_54793| Best HMM Match : No HMM Matches (HMM E-Value=.) 64 2e-10
SB_10256| Best HMM Match : RasGEF (HMM E-Value=1.8e-34) 60 3e-09
SB_2866| Best HMM Match : RasGEF (HMM E-Value=1.3e-26) 60 5e-09
SB_48676| Best HMM Match : No HMM Matches (HMM E-Value=.) 58 1e-08
SB_25728| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 2e-08
SB_54230| Best HMM Match : EGF (HMM E-Value=0) 52 9e-07
SB_5795| Best HMM Match : RasGEF (HMM E-Value=5.3e-06) 48 1e-05
SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24) 46 5e-05
SB_29257| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.005
SB_49937| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.007
SB_8431| Best HMM Match : Spt4 (HMM E-Value=4.6) 36 0.085
SB_7844| Best HMM Match : RasGEF (HMM E-Value=0.00058) 33 0.34
SB_58409| Best HMM Match : DUF947 (HMM E-Value=5.9) 32 0.79
SB_4457| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.79
SB_41416| Best HMM Match : RasGEF (HMM E-Value=1e-04) 31 1.4
SB_32413| Best HMM Match : HEAT (HMM E-Value=0.00039) 30 3.2
SB_58276| Best HMM Match : MIB_HERC2 (HMM E-Value=8.3e-33) 30 3.2
SB_37011| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.6
SB_2107| Best HMM Match : zf-B_box (HMM E-Value=3.9e-08) 29 5.6
SB_871| Best HMM Match : 7tm_1 (HMM E-Value=0.0017) 29 5.6
SB_58556| Best HMM Match : 4F5 (HMM E-Value=5.7) 29 7.3
SB_46318| Best HMM Match : DUF947 (HMM E-Value=1.9) 29 7.3
SB_13429| Best HMM Match : Vicilin_N (HMM E-Value=0.13) 29 7.3
SB_41080| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 9.7
SB_26027| Best HMM Match : DUF164 (HMM E-Value=0.1) 29 9.7
SB_37167| Best HMM Match : RhoGEF (HMM E-Value=1.5e-10) 29 9.7
SB_28402| Best HMM Match : ResIII (HMM E-Value=0.061) 29 9.7
>SB_54793| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 523
Score = 64.1 bits (149), Expect = 2e-10
Identities = 35/107 (32%), Positives = 65/107 (60%), Gaps = 4/107 (3%)
Query: 218 EEFVQTFAPIHPPQSTSACKTSISLHHIDTKCTRNLEAY--ADWFNRLSYLVATDILKGV 275
+E V +P + S+S+ ++ H ++ K R+ + ++FNR++ LVA++IL
Sbjct: 41 DEEVSNESPFNRKWSSSS-SAGMNQHKVE-KYVRSPDVMEMVEFFNRIAMLVASEILAEE 98
Query: 276 KSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMSPVMRLKKTW 322
++ RARV+ + + +C LGN+NSL A+++ L +P+ RLK+TW
Sbjct: 99 TTQARARVVSKVIQIADKCRLLGNYNSLKALMAGLQSAPIYRLKQTW 145
>SB_10256| Best HMM Match : RasGEF (HMM E-Value=1.8e-34)
Length = 792
Score = 60.1 bits (139), Expect = 3e-09
Identities = 36/93 (38%), Positives = 50/93 (53%), Gaps = 4/93 (4%)
Query: 253 LEAYADWFNRLSYLVATDILKGVKSK---YRARVIEQWVMTGRECFNLGNFNSLMAIISA 309
++A D FN +S V + IL+ ++K R R I QW+ + C NL NF+SL AIISA
Sbjct: 35 MKATVDQFNAVSLKVLSSILRTPENKSPAQRGRYINQWINIAQCCRNLKNFSSLKAIISA 94
Query: 310 LNMSPVMRLKKTWSRTSAVCGQQLRQL-ELCVE 341
L + + RLKK+W +L LC E
Sbjct: 95 LQSASIHRLKKSWQHVPRESTALFNELASLCSE 127
>SB_2866| Best HMM Match : RasGEF (HMM E-Value=1.3e-26)
Length = 566
Score = 59.7 bits (138), Expect = 5e-09
Identities = 29/95 (30%), Positives = 50/95 (52%)
Query: 252 NLEAYADWFNRLSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALN 311
NL+ FN + Y V T+I + R ++++++ C + N NS AII ALN
Sbjct: 300 NLDICLSRFNEVHYWVITEICLATQLSKRVFLLKKFIKIASYCKDYKNLNSAFAIIMALN 359
Query: 312 MSPVMRLKKTWSRTSAVCGQQLRQLELCVEPTGNH 346
+ + RL +TW + + R+L+ ++P+GNH
Sbjct: 360 CAAISRLTQTWEKIPNKYKKLYRELDAILDPSGNH 394
>SB_48676| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 453
Score = 58.0 bits (134), Expect = 1e-08
Identities = 42/169 (24%), Positives = 75/169 (44%), Gaps = 17/169 (10%)
Query: 170 EESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIVELHRLSFVGPEEFVQTFAPIHP 229
++ L + S D + + P +A ++T+++ LS +G EE
Sbjct: 50 KDKLCQVVPTLSADGRNNVPFHLADIAPRAMAEEITMIDSEMLSAIGYEEV--------- 100
Query: 230 PQSTSACKTSISLHHIDTKCTRNLEAYADWFNRLSYLVATDILKGVKSKYRARVIEQWVM 289
T +L ID + ++ D++N ++ + AT++L S+ RA VI + +
Sbjct: 101 --------TGGALMSIDKVKSPHVVQMIDFYNSIAAITATEVLVKEGSRDRAYVISRVIQ 152
Query: 290 TGRECFNLGNFNSLMAIISALNMSPVMRLKKTWSRTSAVCGQQLRQLEL 338
EC + NFNS A++S L + RL KTW + + LR L +
Sbjct: 153 MADECVDFNNFNSAKALMSGLRYVAITRLTKTWKKVPEKHKRTLRHLSI 201
>SB_25728| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 250
Score = 57.2 bits (132), Expect = 2e-08
Identities = 31/66 (46%), Positives = 43/66 (65%), Gaps = 8/66 (12%)
Query: 206 IVELHRLSFVGPEEFVQTFAPIHPPQSTSACKTSISLHHIDTKCTRNLEAYADWFNRLSY 265
++ RL+ +GPEEFVQTF P + + K+++S K T +LEAY +WFNRL Y
Sbjct: 4 VLHQERLANIGPEEFVQTFIP----RDEADFKSTLSY----IKRTSSLEAYVEWFNRLGY 55
Query: 266 LVATDI 271
LVAT+I
Sbjct: 56 LVATEI 61
>SB_54230| Best HMM Match : EGF (HMM E-Value=0)
Length = 1359
Score = 52.0 bits (119), Expect = 9e-07
Identities = 34/107 (31%), Positives = 58/107 (54%), Gaps = 2/107 (1%)
Query: 244 HIDTKCTRNLEAYADWFNRLSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSL 303
++D++ E AD FN+LS V IL + RA++ ++ T ++C L NFN++
Sbjct: 400 NLDSQPRLLCERVADRFNQLSTWVVASILTEESVEKRAQMFINFIETAKQCLKLRNFNAV 459
Query: 304 MAI-ISALNMSPVMRLKKTWSRTSAVCGQQLRQLELCVEPTGNHARL 349
MAI ++AL S + RL +T +Q ++E+ ++ T N RL
Sbjct: 460 MAIVVAALGSSSIRRLARTKQLVPKEFLEQCAKIEMLMD-TKNLYRL 505
>SB_5795| Best HMM Match : RasGEF (HMM E-Value=5.3e-06)
Length = 155
Score = 48.4 bits (110), Expect = 1e-05
Identities = 21/64 (32%), Positives = 36/64 (56%)
Query: 263 LSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMSPVMRLKKTW 322
+S+ V +IL K K R V+ ++ + + + N +SL A+I+ L +PV RL +TW
Sbjct: 13 VSFWVVREILNAKKLKTRVAVMSHFIRIAKRLYEMNNLHSLKAVIAGLQSAPVYRLNQTW 72
Query: 323 SRTS 326
+ S
Sbjct: 73 EQLS 76
>SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24)
Length = 315
Score = 46.4 bits (105), Expect = 5e-05
Identities = 24/58 (41%), Positives = 31/58 (53%)
Query: 252 NLEAYADWFNRLSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISA 309
N+ + FN S LV ++ILK RA VIE+W C NL NFNS++ I A
Sbjct: 109 NILRVSQRFNETSALVTSEILKRQSPSARATVIEKWASVAEVCRNLHNFNSVLEITPA 166
>SB_29257| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 494
Score = 39.5 bits (88), Expect = 0.005
Identities = 35/129 (27%), Positives = 65/129 (50%), Gaps = 15/129 (11%)
Query: 195 LTPVELANQLTIVELHRLSFVGPEEFVQTFAPIHPPQSTSACKTSISLHHIDTKCTRNLE 254
L P+E+A QLT++E V P E V + + ++ +HH + K T
Sbjct: 125 LHPIEIARQLTLLESELYRAVRPSELVGSVWTKEDIKHLTSPNLLKMIHHTN-KITV--- 180
Query: 255 AYADWFNRLSYLVATDILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMSP 314
WF + S + +++ + V R+I+ ++T + L NFN ++ ++SALN +P
Sbjct: 181 ----WFEK-SIVEMSNLEERVA--VLTRIID--ILTVFQ--ELNNFNGILEVVSALNSAP 229
Query: 315 VMRLKKTWS 323
+ RL+ T++
Sbjct: 230 IFRLQHTFA 238
>SB_49937| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 850
Score = 39.1 bits (87), Expect = 0.007
Identities = 26/83 (31%), Positives = 41/83 (49%), Gaps = 10/83 (12%)
Query: 252 NLEAYADWFNRLSYLVATDI---------LKGVKSKYR-ARVIEQWVMTGRECFNLGNFN 301
NL + + NR ++ V T+I +K +K + A +++ W + C + N N
Sbjct: 741 NLAKFEETVNRETFWVVTEICGESNVVKRMKLIKHFIKIASMLKTWSFVVQYCKDCKNIN 800
Query: 302 SLMAIISALNMSPVMRLKKTWSR 324
S AIIS L + V RLK TW +
Sbjct: 801 SAFAIISGLGHTAVKRLKNTWEK 823
>SB_8431| Best HMM Match : Spt4 (HMM E-Value=4.6)
Length = 62
Score = 35.5 bits (78), Expect = 0.085
Identities = 19/59 (32%), Positives = 30/59 (50%)
Query: 271 ILKGVKSKYRARVIEQWVMTGRECFNLGNFNSLMAIISALNMSPVMRLKKTWSRTSAVC 329
+L ++ RA+ IE++ + L NFN L+A+ LN S + RL +T S C
Sbjct: 2 VLNHTSAEDRAKCIEKFQKVAQSLKELQNFNGLLAVTGGLNNSVLSRLLQTREYVSKDC 60
>SB_7844| Best HMM Match : RasGEF (HMM E-Value=0.00058)
Length = 1299
Score = 33.5 bits (73), Expect = 0.34
Identities = 13/38 (34%), Positives = 20/38 (52%)
Query: 289 MTGRECFNLGNFNSLMAIISALNMSPVMRLKKTWSRTS 326
M +C + NFNS A++S+ + RL TW + S
Sbjct: 953 MIAEKCALMNNFNSCKALLSSFRFHVISRLSDTWKKVS 990
>SB_58409| Best HMM Match : DUF947 (HMM E-Value=5.9)
Length = 134
Score = 32.3 bits (70), Expect = 0.79
Identities = 19/61 (31%), Positives = 33/61 (54%)
Query: 127 DRVMALVRSITQRCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS 186
DRVM LV +R AAE +ES + ++ +L+++TA E+ + L + S ++
Sbjct: 42 DRVMLLVTKFKRRMAAERLESGINPTMSEVDTMLEEITAREREADLLRETEADGSKKKVE 101
Query: 187 Q 187
Q
Sbjct: 102 Q 102
>SB_4457| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1328
Score = 32.3 bits (70), Expect = 0.79
Identities = 17/46 (36%), Positives = 28/46 (60%), Gaps = 4/46 (8%)
Query: 66 KAIPNHPY----TNCTTEKSDIEYRDGQIGKAEVKDLVEKELNDMI 107
KA+ N P+ ++ +T KS + ++ G+ GK K L +KE ND+I
Sbjct: 759 KAVENKPFGRPASHVSTIKSFVGFKKGKDGKPTKKQLKDKEANDVI 804
>SB_41416| Best HMM Match : RasGEF (HMM E-Value=1e-04)
Length = 141
Score = 31.5 bits (68), Expect = 1.4
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Query: 293 ECFNLGNFNSLMAI-ISALNMSPVMRLKKTWSRTSAVCGQQLRQLELCVE 341
+C L NFN++MAI ++AL S + RL +T +Q ++E+ ++
Sbjct: 1 QCLKLRNFNAVMAIVVAALGSSSIRRLARTKQLVPKEFLEQCAKIEMLMD 50
>SB_32413| Best HMM Match : HEAT (HMM E-Value=0.00039)
Length = 695
Score = 30.3 bits (65), Expect = 3.2
Identities = 13/37 (35%), Positives = 22/37 (59%)
Query: 139 RCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESLVD 175
RC +H++S+ VE L+ LL ++TA QY + +
Sbjct: 487 RCLGQHLKSLGVEGEELLDTLLPEVTAFCQYIQGFTE 523
>SB_58276| Best HMM Match : MIB_HERC2 (HMM E-Value=8.3e-33)
Length = 2822
Score = 30.3 bits (65), Expect = 3.2
Identities = 15/51 (29%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Query: 300 FNSLMAIISALNMSPVMRLKKTWSRTSAVCGQQLRQLELCVEPT--GNHAR 348
F+ + + A + R+++ W T V G+++R +ELCV + G AR
Sbjct: 1706 FSYVQRVTLAAGGTKTERIRRLWEPTYTVFGERVRFMELCVRDSVVGEQAR 1756
>SB_37011| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1829
Score = 29.5 bits (63), Expect = 5.6
Identities = 19/73 (26%), Positives = 40/73 (54%), Gaps = 7/73 (9%)
Query: 124 FRDDRVMALVRSITQRCA---AEHMESVRVEMSTTLEKL---LDKLTALEQYEESLVDIT 177
++D RV+A + + + C EH + V EK+ ++++ +L+ + L+D+
Sbjct: 24 YQDPRVLACLHTYCRHCLESLVEHSKECTVSCPQCREKIEISVEEVKSLK-VDFMLIDLI 82
Query: 178 QVSSLDQLSQGDI 190
+ S+DQ ++GDI
Sbjct: 83 EKMSVDQENKGDI 95
>SB_2107| Best HMM Match : zf-B_box (HMM E-Value=3.9e-08)
Length = 599
Score = 29.5 bits (63), Expect = 5.6
Identities = 19/73 (26%), Positives = 40/73 (54%), Gaps = 7/73 (9%)
Query: 124 FRDDRVMALVRSITQRCA---AEHMESVRVEMSTTLEKL---LDKLTALEQYEESLVDIT 177
++D RV+A + + + C EH + V EK+ ++++ +L+ + L+D+
Sbjct: 33 YQDPRVLACLHTYCRHCLESLVEHSKECTVSCPQCREKIEISVEEVKSLK-VDFMLIDLI 91
Query: 178 QVSSLDQLSQGDI 190
+ S+DQ ++GDI
Sbjct: 92 EKMSVDQENKGDI 104
>SB_871| Best HMM Match : 7tm_1 (HMM E-Value=0.0017)
Length = 1675
Score = 29.5 bits (63), Expect = 5.6
Identities = 23/93 (24%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Query: 148 VRVEMSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLSQGDILTLGLTPVELANQLTIV 207
V + M+ ++ D+L L + E L ++ V + + QGD + TP++++ I+
Sbjct: 543 VALNMAQNVDLSRDELAGLAKGETPLATVSAVKLVQFILQGDGPS-SSTPIDISTIHKIL 601
Query: 208 ELHRLSFVGPEEF-VQTFAPIHPPQSTSACKTS 239
H + +G +F +QT+ I CKT+
Sbjct: 602 RPHSIR-IGSIDFDIQTWCSIIQAFQFHNCKTA 633
>SB_58556| Best HMM Match : 4F5 (HMM E-Value=5.7)
Length = 215
Score = 29.1 bits (62), Expect = 7.3
Identities = 18/61 (29%), Positives = 31/61 (50%)
Query: 127 DRVMALVRSITQRCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS 186
DRVM LV +R AAE ES + ++ +L+++ A E+ + L + S ++
Sbjct: 42 DRVMLLVTKFKRRMAAERRESGINPTMSEVDTMLEEIAAREREADLLRETEADGSKKKVE 101
Query: 187 Q 187
Q
Sbjct: 102 Q 102
>SB_46318| Best HMM Match : DUF947 (HMM E-Value=1.9)
Length = 215
Score = 29.1 bits (62), Expect = 7.3
Identities = 18/61 (29%), Positives = 31/61 (50%)
Query: 127 DRVMALVRSITQRCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS 186
DRVM LV +R AAE ES + ++ +L+++ A E+ + L + S ++
Sbjct: 42 DRVMLLVTKFKRRMAAERRESGINPTMSEVDTMLEEIAAREREADLLRETEADGSKKKVE 101
Query: 187 Q 187
Q
Sbjct: 102 Q 102
>SB_13429| Best HMM Match : Vicilin_N (HMM E-Value=0.13)
Length = 323
Score = 29.1 bits (62), Expect = 7.3
Identities = 18/61 (29%), Positives = 31/61 (50%)
Query: 127 DRVMALVRSITQRCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS 186
DRVM LV +R AAE ES + ++ +L+++ A E+ + L + S ++
Sbjct: 64 DRVMLLVTKFKRRMAAERRESGINPTMSEVDTMLEEIAAQEKEADLLRETEADGSKKKVE 123
Query: 187 Q 187
Q
Sbjct: 124 Q 124
>SB_41080| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 93
Score = 28.7 bits (61), Expect = 9.7
Identities = 16/47 (34%), Positives = 26/47 (55%)
Query: 127 DRVMALVRSITQRCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESL 173
DRVM LV +R AAE ES + ++ +L+++ A E+ + L
Sbjct: 42 DRVMLLVTKFKRRMAAERRESGINPTMSEVDTMLEEIAAREREADLL 88
>SB_26027| Best HMM Match : DUF164 (HMM E-Value=0.1)
Length = 715
Score = 28.7 bits (61), Expect = 9.7
Identities = 25/109 (22%), Positives = 51/109 (46%), Gaps = 7/109 (6%)
Query: 143 EHMESVRVEMSTTLEKLLDKLTALEQYEESLVDITQVSSLDQLS-QGDILTLGLTPVELA 201
+ +E E+++ L + LD L L+Q E+ ++ ++L+++S Q D+L L +
Sbjct: 202 DDLELENSELTSQLGRSLDNLKQLDQIHENTA-VSSENALERMSGQVDVLNTALEKLREE 260
Query: 202 NQLTIVELHRLSFVGPEEFVQTFAPIHPPQSTSACKTSISLHHIDTKCT 250
N +L + ++ + P P+++SA + I L T T
Sbjct: 261 NSRLKSKLGAI-----KDHAWEYVPKETPKTSSASQKVIKLSSPSTDLT 304
>SB_37167| Best HMM Match : RhoGEF (HMM E-Value=1.5e-10)
Length = 180
Score = 28.7 bits (61), Expect = 9.7
Identities = 13/35 (37%), Positives = 20/35 (57%)
Query: 139 RCAAEHMESVRVEMSTTLEKLLDKLTALEQYEESL 173
+C AEH E ++ ST + D + LE+YE+ L
Sbjct: 29 KCFAEHAEEIKSVYSTYCKNHDDAIGLLEKYEDCL 63
>SB_28402| Best HMM Match : ResIII (HMM E-Value=0.061)
Length = 337
Score = 28.7 bits (61), Expect = 9.7
Identities = 14/39 (35%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
Query: 151 EMSTTLEKLLDKLTALE--QYEESLVDITQVSSLDQLSQ 187
E+ +EK K T +E Q EE L+D+ ++ S+ ++SQ
Sbjct: 84 ELEVKMEKKKSKSTKVEVEQLEEELLDLGEIESIKKISQ 122
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.131 0.383
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,100,873
Number of Sequences: 59808
Number of extensions: 468821
Number of successful extensions: 1117
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1092
Number of HSP's gapped (non-prelim): 29
length of query: 433
length of database: 16,821,457
effective HSP length: 84
effective length of query: 349
effective length of database: 11,797,585
effective search space: 4117357165
effective search space used: 4117357165
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 61 (28.7 bits)
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