Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000518-TA|BGIBMGA000518-PA|IPR001932|Protein phosphatase
2C-like, IPR000222|Protein phosphatase 2C
         (445 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase...   167   4e-42
SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomy...    52   2e-07
SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy...    48   2e-06
SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|c...    33   0.061
SPCC4F11.02 |ptc1||protein phosphatase 2C Ptc1|Schizosaccharomyc...    31   0.25 
SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    31   0.32 
SPAC4A8.03c |ptc4||protein phosphatase 2C Ptc4|Schizosaccharomyc...    30   0.75 
SPAC9G1.12 |cpd1||tRNA |Schizosaccharomyces pombe|chr 1|||Manual       28   2.3  
SPAC3G6.03c |||Maf-like protein|Schizosaccharomyces pombe|chr 1|...    27   4.0  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    27   5.3  
SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces ...    27   7.0  
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa...    26   9.2  
SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo...    26   9.2  
SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo...    26   9.2  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    26   9.2  

>SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 444

 Score =  167 bits (405), Expect = 4e-42
 Identities = 116/299 (38%), Positives = 163/299 (54%), Gaps = 30/299 (10%)

Query: 152 ALEQAIIKIDQDLSKEAVEPYKLNQVINPQTLSVA-------LSGAVACVTHVDGPN--L 202
           ++ +A  K+D  +  E V  +  N   NP++L VA       LSG+ A +T     +  L
Sbjct: 166 SISEAFAKVDHQIVHEHVS-HVFN---NPESLQVAASLLLPALSGSCALLTSYSAKSKSL 221

Query: 203 HIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDRLL 262
            +A  GD  AVLG  T D  W A  ++++    N DE  R+  EHP  E   V+R +R+L
Sbjct: 222 QVACTGDSRAVLGECTPDGSWEAIPLSRDQTGMNPDEASRLEVEHPGEE---VLRNNRIL 278

Query: 263 GELAPLRSLGDYRYKWPIEILMKVAVPLIGPKAIPANYHTPPYLTAKPDLYYHRLSTRD- 321
           G L P R+ GD RYKW  EI  ++         IP    TPPY+TA P++    ++ +  
Sbjct: 279 GRLMPSRAFGDARYKWSQEISERLHREYFSASPIPVK--TPPYVTAVPEIESITVNPKKH 336

Query: 322 KFLILASDGLWDMMTPLQAVKLVGEHMKGKVFFNPLKLPRKNIQLGDINELLLHRKESLK 381
           +FLI+ASDGLWD M+  QAV+LVGE     V         KN    D     L +K    
Sbjct: 337 RFLIMASDGLWDTMSSEQAVQLVGE-WADTVLGKTTN--EKNTTQDDKQSWSLFKK---T 390

Query: 382 SKPKDRNAATHLIRHAIGGTEYGVDHSRLAHLLSLSPDVSRMFRDDITVTVMYFDSEFL 440
           SK  D NAATHLIRH++GG++      R++ LL+L+  +SR +RDDITVTV++FD + L
Sbjct: 391 SKVIDDNAATHLIRHSLGGSD-----QRISALLTLTYPISRRYRDDITVTVIFFDEKTL 444


>SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 414

 Score = 52.0 bits (119), Expect = 2e-07
 Identities = 48/157 (30%), Positives = 69/157 (43%), Gaps = 29/157 (18%)

Query: 188 SGAVACVTHVDGPNLHIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEH 247
           SG  A V    G  L+ AN GD   VLG      + IAK ++ +H   N  E  RI    
Sbjct: 118 SGCTATVVLRVGNKLYCANAGDSRTVLG-----SKGIAKPLSADHKPSNEAEKARIC--- 169

Query: 248 PDNERKTVIRRDRLLGELAPLRSLGDYRYKWPIEILMKVAVPLIGPKAIPANYHTPPYLT 307
                   +   R+ G LA  R++GD+ +K            L   K I         +T
Sbjct: 170 ---AAGGFVDFGRVNGNLALSRAIGDFEFK---------NSNLEPEKQI---------VT 208

Query: 308 AKPDLYYHRLSTRDKFLILASDGLWDMMTPLQAVKLV 344
           A PD+  H ++  D+F++LA DG+WD  T  Q ++ V
Sbjct: 209 ALPDVVVHEITDDDEFVVLACDGIWDCKTSQQVIEFV 245


>SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 370

 Score = 48.4 bits (110), Expect = 2e-06
 Identities = 44/148 (29%), Positives = 65/148 (43%), Gaps = 28/148 (18%)

Query: 197 VDGPNLHIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVI 256
           VD   ++ AN GD   VLG     ++  A+ ++ +H   N  E  RI            I
Sbjct: 129 VDHQVIYCANAGDSRTVLG-----RKGTAEPLSFDHKPNNDVEKARI------TAAGGFI 177

Query: 257 RRDRLLGELAPLRSLGDYRYKWPIEILMKVAVPLIGPKAIPANYHTPPYLTAKPDLYYHR 316
              R+ G LA  R++GD+ YK                 ++P        +TA PD+  H 
Sbjct: 178 DFGRVNGSLALSRAIGDFEYK--------------KDSSLPPEKQI---VTAFPDVVIHN 220

Query: 317 LSTRDKFLILASDGLWDMMTPLQAVKLV 344
           +   D+FLILA DG+WD  +  Q V+ V
Sbjct: 221 IDPDDEFLILACDGIWDCKSSQQVVEFV 248



 Score = 27.9 bits (59), Expect = 3.0
 Identities = 12/23 (52%), Positives = 14/23 (60%)

Query: 66 GVFDGHGGPACAQVISKRLVDYI 88
          GVFDGHGG   A+   + L D I
Sbjct: 60 GVFDGHGGDRVAKYCRQHLPDII 82


>SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 801

 Score = 33.5 bits (73), Expect = 0.061
 Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 3/62 (4%)

Query: 187 LSGAVACVTHVDGPNLH--IANVGDCNAVLGILTEDKEWIAKKITKEHNSE-NFDELKRI 243
           L  AV  V  +  PN+   + ++GD N    I TE+K W+++ +  + N   +F+ L  I
Sbjct: 254 LFDAVEEVFILQDPNVEASVFDLGDWNYWFTISTEEKTWLSQSVDADMNPVFSFEHLSFI 313

Query: 244 WN 245
           WN
Sbjct: 314 WN 315


>SPCC4F11.02 |ptc1||protein phosphatase 2C Ptc1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 347

 Score = 31.5 bits (68), Expect = 0.25
 Identities = 12/24 (50%), Positives = 18/24 (75%)

Query: 321 DKFLILASDGLWDMMTPLQAVKLV 344
           D+F I+A DGLWD+++  +AV  V
Sbjct: 267 DEFFIIACDGLWDVVSDQEAVDFV 290


>SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 395

 Score = 31.1 bits (67), Expect = 0.32
 Identities = 22/71 (30%), Positives = 38/71 (53%), Gaps = 5/71 (7%)

Query: 112 DTFNNRVEILQELKILYERSFRQYLAE---LANLPHEGYNVPTALEQAI--IKIDQDLSK 166
           + FNNR +  +E +    +  ++  +E    AN  HE   +  +LE+ +  I +++ L +
Sbjct: 266 ENFNNRFKEFEEREKSAIKQNKEQSSEGSKTANQTHEQKELINSLEKKVSDIALEKQLLE 325

Query: 167 EAVEPYKLNQV 177
           EAVE YKL  V
Sbjct: 326 EAVERYKLAMV 336


>SPAC4A8.03c |ptc4||protein phosphatase 2C Ptc4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 383

 Score = 29.9 bits (64), Expect = 0.75
 Identities = 11/24 (45%), Positives = 15/24 (62%)

Query: 61  SGLIMGVFDGHGGPACAQVISKRL 84
           S    G+FDGHGG  C++ +S  L
Sbjct: 84  SPFFYGLFDGHGGTECSEFLSTNL 107



 Score = 27.5 bits (58), Expect = 4.0
 Identities = 20/77 (25%), Positives = 39/77 (50%), Gaps = 6/77 (7%)

Query: 202 LHIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDRL 261
           +H+A+VGD  A   +L + +   A ++T +H+  + +E +R+   +    R +     + 
Sbjct: 209 IHLAHVGDTRA---LLCDSRTGRAHRLTFQHHPADVEEARRLRRYNMGFSRDSF--GQKR 263

Query: 262 LGELAPLRSLGD-YRYK 277
              +A  RS GD Y+ K
Sbjct: 264 FAWVANTRSFGDGYKLK 280


>SPAC9G1.12 |cpd1||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 364

 Score = 28.3 bits (60), Expect = 2.3
 Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 4/112 (3%)

Query: 151 TALEQAIIKIDQDLSKEAVEPYKLNQVINPQTLSVALSGAVACVTHVDGPNLHIANVGDC 210
           T ++   I +D     EA+ P+  N V + ++  +       C+  +      +  +G C
Sbjct: 176 TEVKVDAIFLDLPAPWEAI-PHLSNHVNHDKSTRICCFSP--CIEQIQHSAEALRELGWC 232

Query: 211 NAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDRLL 262
           +  + I  + K+W A+K    H  E  D LK +     +   +  +RR++ L
Sbjct: 233 DIEM-IEVDYKQWAARKSRIVHIDEAIDRLKEVKRRRIEGFERRKMRREQNL 283


>SPAC3G6.03c |||Maf-like protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 236

 Score = 27.5 bits (58), Expect = 4.0
 Identities = 11/29 (37%), Positives = 17/29 (58%)

Query: 144 HEGYNVPTALEQAIIKIDQDLSKEAVEPY 172
           H GY + T LE+  +K D  ++ E +E Y
Sbjct: 150 HPGYVMKTHLEETQVKFDPSITDEFLEAY 178


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 27.1 bits (57), Expect = 5.3
 Identities = 9/24 (37%), Positives = 16/24 (66%)

Query: 229 TKEHNSENFDELKRIWNEHPDNER 252
           T    S+N D L  +WNE+P++++
Sbjct: 32  TGTEGSKNSDTLLELWNENPESQK 55


>SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 296

 Score = 26.6 bits (56), Expect = 7.0
 Identities = 14/48 (29%), Positives = 24/48 (50%)

Query: 384 PKDRNAATHLIRHAIGGTEYGVDHSRLAHLLSLSPDVSRMFRDDITVT 431
           PK+  A + +  + +  +     HSRLA +LS    + R+ + D T T
Sbjct: 84  PKNYQARSFVKENELYESSQDCGHSRLAQILSEENQIKRLPQSDNTTT 131


>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1628

 Score = 26.2 bits (55), Expect = 9.2
 Identities = 30/110 (27%), Positives = 42/110 (38%), Gaps = 1/110 (0%)

Query: 82   KRLVDYIAAALLPIETLHKYIQQDPREALVDTFNNRVEILQELKILYERSFRQYLAELAN 141
            K L  Y  + LL  E +H Y      E ++  F   +E    LK   ER   +   E   
Sbjct: 1210 KYLTSYFESCLLSTEYMHIYNSVIILEKILPCFPLIIESGSALKRAAERLKDEEKREDLK 1269

Query: 142  LPHEGYNVPTALEQAIIKIDQDLSKEAVEPYKLNQVINPQTLSVALSGAV 191
            +   GY    + +Q         S   V P    ++  PQ LSVA + AV
Sbjct: 1270 VLALGYFAKLSKKQPEWVSFNSFSG-TVRPSNSEKLQRPQQLSVAATSAV 1318


>SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1513

 Score = 26.2 bits (55), Expect = 9.2
 Identities = 15/52 (28%), Positives = 26/52 (50%)

Query: 80  ISKRLVDYIAAALLPIETLHKYIQQDPREALVDTFNNRVEILQELKILYERS 131
           +SK  + Y   +LL  E ++  + ++    +V   NN  EI  ++  L ERS
Sbjct: 935 LSKSNISYDKLSLLRDEAMNLCVNKELFSKVVGILNNAEEIKNKIATLCERS 986


>SPBC947.07 |||ribosome biogenesis protein
           Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 233

 Score = 26.2 bits (55), Expect = 9.2
 Identities = 12/42 (28%), Positives = 20/42 (47%), Gaps = 1/42 (2%)

Query: 219 EDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDR 260
           +D E + KK  +    E   +    W E  DNE+K ++ R +
Sbjct: 163 KDNEQLLKKSIRRKEKEK-KKSSDAWKERKDNEKKAMLMRQQ 203


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 26.2 bits (55), Expect = 9.2
 Identities = 12/47 (25%), Positives = 22/47 (46%)

Query: 60  TSGLIMGVFDGHGGPACAQVISKRLVDYIAAALLPIETLHKYIQQDP 106
           T G ++  +D   G  CA  +++ +  Y  A+L+ +  L      DP
Sbjct: 372 TFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDP 418


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.320    0.137    0.409 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,122,419
Number of Sequences: 5004
Number of extensions: 92192
Number of successful extensions: 273
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 247
Number of HSP's gapped (non-prelim): 25
length of query: 445
length of database: 2,362,478
effective HSP length: 75
effective length of query: 370
effective length of database: 1,987,178
effective search space: 735255860
effective search space used: 735255860
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 55 (26.2 bits)