BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000518-TA|BGIBMGA000518-PA|IPR001932|Protein phosphatase 2C-like, IPR000222|Protein phosphatase 2C (445 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 167 4e-42 SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomy... 52 2e-07 SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy... 48 2e-06 SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|c... 33 0.061 SPCC4F11.02 |ptc1||protein phosphatase 2C Ptc1|Schizosaccharomyc... 31 0.25 SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 31 0.32 SPAC4A8.03c |ptc4||protein phosphatase 2C Ptc4|Schizosaccharomyc... 30 0.75 SPAC9G1.12 |cpd1||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 28 2.3 SPAC3G6.03c |||Maf-like protein|Schizosaccharomyces pombe|chr 1|... 27 4.0 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 5.3 SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces ... 27 7.0 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 26 9.2 SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 26 9.2 SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 26 9.2 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 9.2 >SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 444 Score = 167 bits (405), Expect = 4e-42 Identities = 116/299 (38%), Positives = 163/299 (54%), Gaps = 30/299 (10%) Query: 152 ALEQAIIKIDQDLSKEAVEPYKLNQVINPQTLSVA-------LSGAVACVTHVDGPN--L 202 ++ +A K+D + E V + N NP++L VA LSG+ A +T + L Sbjct: 166 SISEAFAKVDHQIVHEHVS-HVFN---NPESLQVAASLLLPALSGSCALLTSYSAKSKSL 221 Query: 203 HIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDRLL 262 +A GD AVLG T D W A ++++ N DE R+ EHP E V+R +R+L Sbjct: 222 QVACTGDSRAVLGECTPDGSWEAIPLSRDQTGMNPDEASRLEVEHPGEE---VLRNNRIL 278 Query: 263 GELAPLRSLGDYRYKWPIEILMKVAVPLIGPKAIPANYHTPPYLTAKPDLYYHRLSTRD- 321 G L P R+ GD RYKW EI ++ IP TPPY+TA P++ ++ + Sbjct: 279 GRLMPSRAFGDARYKWSQEISERLHREYFSASPIPVK--TPPYVTAVPEIESITVNPKKH 336 Query: 322 KFLILASDGLWDMMTPLQAVKLVGEHMKGKVFFNPLKLPRKNIQLGDINELLLHRKESLK 381 +FLI+ASDGLWD M+ QAV+LVGE V KN D L +K Sbjct: 337 RFLIMASDGLWDTMSSEQAVQLVGE-WADTVLGKTTN--EKNTTQDDKQSWSLFKK---T 390 Query: 382 SKPKDRNAATHLIRHAIGGTEYGVDHSRLAHLLSLSPDVSRMFRDDITVTVMYFDSEFL 440 SK D NAATHLIRH++GG++ R++ LL+L+ +SR +RDDITVTV++FD + L Sbjct: 391 SKVIDDNAATHLIRHSLGGSD-----QRISALLTLTYPISRRYRDDITVTVIFFDEKTL 444 >SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomyces pombe|chr 1|||Manual Length = 414 Score = 52.0 bits (119), Expect = 2e-07 Identities = 48/157 (30%), Positives = 69/157 (43%), Gaps = 29/157 (18%) Query: 188 SGAVACVTHVDGPNLHIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEH 247 SG A V G L+ AN GD VLG + IAK ++ +H N E RI Sbjct: 118 SGCTATVVLRVGNKLYCANAGDSRTVLG-----SKGIAKPLSADHKPSNEAEKARIC--- 169 Query: 248 PDNERKTVIRRDRLLGELAPLRSLGDYRYKWPIEILMKVAVPLIGPKAIPANYHTPPYLT 307 + R+ G LA R++GD+ +K L K I +T Sbjct: 170 ---AAGGFVDFGRVNGNLALSRAIGDFEFK---------NSNLEPEKQI---------VT 208 Query: 308 AKPDLYYHRLSTRDKFLILASDGLWDMMTPLQAVKLV 344 A PD+ H ++ D+F++LA DG+WD T Q ++ V Sbjct: 209 ALPDVVVHEITDDDEFVVLACDGIWDCKTSQQVIEFV 245 >SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 370 Score = 48.4 bits (110), Expect = 2e-06 Identities = 44/148 (29%), Positives = 65/148 (43%), Gaps = 28/148 (18%) Query: 197 VDGPNLHIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVI 256 VD ++ AN GD VLG ++ A+ ++ +H N E RI I Sbjct: 129 VDHQVIYCANAGDSRTVLG-----RKGTAEPLSFDHKPNNDVEKARI------TAAGGFI 177 Query: 257 RRDRLLGELAPLRSLGDYRYKWPIEILMKVAVPLIGPKAIPANYHTPPYLTAKPDLYYHR 316 R+ G LA R++GD+ YK ++P +TA PD+ H Sbjct: 178 DFGRVNGSLALSRAIGDFEYK--------------KDSSLPPEKQI---VTAFPDVVIHN 220 Query: 317 LSTRDKFLILASDGLWDMMTPLQAVKLV 344 + D+FLILA DG+WD + Q V+ V Sbjct: 221 IDPDDEFLILACDGIWDCKSSQQVVEFV 248 Score = 27.9 bits (59), Expect = 3.0 Identities = 12/23 (52%), Positives = 14/23 (60%) Query: 66 GVFDGHGGPACAQVISKRLVDYI 88 GVFDGHGG A+ + L D I Sbjct: 60 GVFDGHGGDRVAKYCRQHLPDII 82 >SPBC1685.14c |||Vid27 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 801 Score = 33.5 bits (73), Expect = 0.061 Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 3/62 (4%) Query: 187 LSGAVACVTHVDGPNLH--IANVGDCNAVLGILTEDKEWIAKKITKEHNSE-NFDELKRI 243 L AV V + PN+ + ++GD N I TE+K W+++ + + N +F+ L I Sbjct: 254 LFDAVEEVFILQDPNVEASVFDLGDWNYWFTISTEEKTWLSQSVDADMNPVFSFEHLSFI 313 Query: 244 WN 245 WN Sbjct: 314 WN 315 >SPCC4F11.02 |ptc1||protein phosphatase 2C Ptc1|Schizosaccharomyces pombe|chr 3|||Manual Length = 347 Score = 31.5 bits (68), Expect = 0.25 Identities = 12/24 (50%), Positives = 18/24 (75%) Query: 321 DKFLILASDGLWDMMTPLQAVKLV 344 D+F I+A DGLWD+++ +AV V Sbjct: 267 DEFFIIACDGLWDVVSDQEAVDFV 290 >SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 31.1 bits (67), Expect = 0.32 Identities = 22/71 (30%), Positives = 38/71 (53%), Gaps = 5/71 (7%) Query: 112 DTFNNRVEILQELKILYERSFRQYLAE---LANLPHEGYNVPTALEQAI--IKIDQDLSK 166 + FNNR + +E + + ++ +E AN HE + +LE+ + I +++ L + Sbjct: 266 ENFNNRFKEFEEREKSAIKQNKEQSSEGSKTANQTHEQKELINSLEKKVSDIALEKQLLE 325 Query: 167 EAVEPYKLNQV 177 EAVE YKL V Sbjct: 326 EAVERYKLAMV 336 >SPAC4A8.03c |ptc4||protein phosphatase 2C Ptc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 29.9 bits (64), Expect = 0.75 Identities = 11/24 (45%), Positives = 15/24 (62%) Query: 61 SGLIMGVFDGHGGPACAQVISKRL 84 S G+FDGHGG C++ +S L Sbjct: 84 SPFFYGLFDGHGGTECSEFLSTNL 107 Score = 27.5 bits (58), Expect = 4.0 Identities = 20/77 (25%), Positives = 39/77 (50%), Gaps = 6/77 (7%) Query: 202 LHIANVGDCNAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDRL 261 +H+A+VGD A +L + + A ++T +H+ + +E +R+ + R + + Sbjct: 209 IHLAHVGDTRA---LLCDSRTGRAHRLTFQHHPADVEEARRLRRYNMGFSRDSF--GQKR 263 Query: 262 LGELAPLRSLGD-YRYK 277 +A RS GD Y+ K Sbjct: 264 FAWVANTRSFGDGYKLK 280 >SPAC9G1.12 |cpd1||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 364 Score = 28.3 bits (60), Expect = 2.3 Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 4/112 (3%) Query: 151 TALEQAIIKIDQDLSKEAVEPYKLNQVINPQTLSVALSGAVACVTHVDGPNLHIANVGDC 210 T ++ I +D EA+ P+ N V + ++ + C+ + + +G C Sbjct: 176 TEVKVDAIFLDLPAPWEAI-PHLSNHVNHDKSTRICCFSP--CIEQIQHSAEALRELGWC 232 Query: 211 NAVLGILTEDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDRLL 262 + + I + K+W A+K H E D LK + + + +RR++ L Sbjct: 233 DIEM-IEVDYKQWAARKSRIVHIDEAIDRLKEVKRRRIEGFERRKMRREQNL 283 >SPAC3G6.03c |||Maf-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 27.5 bits (58), Expect = 4.0 Identities = 11/29 (37%), Positives = 17/29 (58%) Query: 144 HEGYNVPTALEQAIIKIDQDLSKEAVEPY 172 H GY + T LE+ +K D ++ E +E Y Sbjct: 150 HPGYVMKTHLEETQVKFDPSITDEFLEAY 178 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 27.1 bits (57), Expect = 5.3 Identities = 9/24 (37%), Positives = 16/24 (66%) Query: 229 TKEHNSENFDELKRIWNEHPDNER 252 T S+N D L +WNE+P++++ Sbjct: 32 TGTEGSKNSDTLLELWNENPESQK 55 >SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 296 Score = 26.6 bits (56), Expect = 7.0 Identities = 14/48 (29%), Positives = 24/48 (50%) Query: 384 PKDRNAATHLIRHAIGGTEYGVDHSRLAHLLSLSPDVSRMFRDDITVT 431 PK+ A + + + + + HSRLA +LS + R+ + D T T Sbjct: 84 PKNYQARSFVKENELYESSQDCGHSRLAQILSEENQIKRLPQSDNTTT 131 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 26.2 bits (55), Expect = 9.2 Identities = 30/110 (27%), Positives = 42/110 (38%), Gaps = 1/110 (0%) Query: 82 KRLVDYIAAALLPIETLHKYIQQDPREALVDTFNNRVEILQELKILYERSFRQYLAELAN 141 K L Y + LL E +H Y E ++ F +E LK ER + E Sbjct: 1210 KYLTSYFESCLLSTEYMHIYNSVIILEKILPCFPLIIESGSALKRAAERLKDEEKREDLK 1269 Query: 142 LPHEGYNVPTALEQAIIKIDQDLSKEAVEPYKLNQVINPQTLSVALSGAV 191 + GY + +Q S V P ++ PQ LSVA + AV Sbjct: 1270 VLALGYFAKLSKKQPEWVSFNSFSG-TVRPSNSEKLQRPQQLSVAATSAV 1318 >SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1513 Score = 26.2 bits (55), Expect = 9.2 Identities = 15/52 (28%), Positives = 26/52 (50%) Query: 80 ISKRLVDYIAAALLPIETLHKYIQQDPREALVDTFNNRVEILQELKILYERS 131 +SK + Y +LL E ++ + ++ +V NN EI ++ L ERS Sbjct: 935 LSKSNISYDKLSLLRDEAMNLCVNKELFSKVVGILNNAEEIKNKIATLCERS 986 >SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual Length = 233 Score = 26.2 bits (55), Expect = 9.2 Identities = 12/42 (28%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Query: 219 EDKEWIAKKITKEHNSENFDELKRIWNEHPDNERKTVIRRDR 260 +D E + KK + E + W E DNE+K ++ R + Sbjct: 163 KDNEQLLKKSIRRKEKEK-KKSSDAWKERKDNEKKAMLMRQQ 203 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 26.2 bits (55), Expect = 9.2 Identities = 12/47 (25%), Positives = 22/47 (46%) Query: 60 TSGLIMGVFDGHGGPACAQVISKRLVDYIAAALLPIETLHKYIQQDP 106 T G ++ +D G CA +++ + Y A+L+ + L DP Sbjct: 372 TFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDP 418 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.320 0.137 0.409 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,122,419 Number of Sequences: 5004 Number of extensions: 92192 Number of successful extensions: 273 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 247 Number of HSP's gapped (non-prelim): 25 length of query: 445 length of database: 2,362,478 effective HSP length: 75 effective length of query: 370 effective length of database: 1,987,178 effective search space: 735255860 effective search space used: 735255860 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 55 (26.2 bits)
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