Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000506-TA|BGIBMGA000506-
PA|IPR003140|Phospholipase/Carboxylesterase
(126 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g22300.1 68417.m03225 phospholipase/carboxylesterase family p... 51 2e-07
At3g15650.1 68416.m01984 phospholipase/carboxylesterase family p... 41 2e-04
At1g52700.1 68414.m05952 phospholipase/carboxylesterase family p... 40 4e-04
At5g20060.2 68418.m02389 phospholipase/carboxylesterase family p... 38 0.003
At5g20060.1 68418.m02388 phospholipase/carboxylesterase family p... 38 0.003
At1g62870.1 68414.m07099 expressed protein 31 0.23
At1g52460.1 68414.m05922 hypothetical protein 30 0.54
At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1) ide... 29 0.71
At2g24320.1 68415.m02907 hypothetical protein 28 1.6
At1g12380.1 68414.m01431 expressed protein 28 2.2
At1g31920.1 68414.m03922 pentatricopeptide (PPR) repeat-containi... 27 2.9
At1g51860.1 68414.m05846 leucine-rich repeat protein kinase, put... 27 3.8
At1g09320.1 68414.m01043 agenet domain-containing protein contai... 27 3.8
At4g33180.1 68417.m04726 hydrolase, alpha/beta fold family prote... 27 5.0
At3g30380.1 68416.m03835 expressed protein ; expression supporte... 27 5.0
At3g14730.1 68416.m01862 pentatricopeptide (PPR) repeat-containi... 27 5.0
At5g66470.1 68418.m08382 expressed protein 26 6.6
At4g24760.1 68417.m03545 expressed protein 26 6.6
At4g11050.1 68417.m01796 endo-1,4-beta-glucanase, putative / cel... 26 6.6
At2g04300.1 68415.m00422 leucine-rich repeat protein kinase, put... 26 6.6
At1g44170.2 68414.m05102 aldehyde dehydrogenase, putative (ALDH)... 26 6.6
At1g44170.1 68414.m05101 aldehyde dehydrogenase, putative (ALDH)... 26 6.6
At3g01690.1 68416.m00101 expressed protein 26 8.8
At1g73200.1 68414.m08471 expressed protein 26 8.8
At1g52695.1 68414.m05951 phospholipase/carboxylesterase family p... 26 8.8
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 26 8.8
>At4g22300.1 68417.m03225 phospholipase/carboxylesterase family
protein similar to acyl-protein thioesterase-1 [Homo
sapiens] GI:9965372; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 471
Score = 51.2 bits (117), Expect = 2e-07
Identities = 32/116 (27%), Positives = 54/116 (46%), Gaps = 7/116 (6%)
Query: 15 GFSMGGALAFHTGYRWDRKLAGVFAFSSFLNYNSAVY----DELKNNTGVTY---PPLLQ 67
G S GGAL + + + L G S ++ + S++ +E K + + P+L
Sbjct: 104 GLSQGGALTLASVLLYPKTLGGGAVLSGWVPFTSSIISQFPEEAKKVPHLCFLINTPILW 163
Query: 68 IHGNQDDLVPLKWGEETYQKLKSLGIQGSFFVQERLGHSLNRRGIKIIKDWIDEKL 123
HG D +V + G+ LK G+ F LGHS++ + +K I+ WI +L
Sbjct: 164 SHGTDDRMVLFEAGQAALPFLKEAGVTCEFKAYPGLGHSISNKELKYIESWIKRRL 219
Score = 41.1 bits (92), Expect = 2e-04
Identities = 24/85 (28%), Positives = 41/85 (48%), Gaps = 2/85 (2%)
Query: 15 GFSMGGALAFHTGYRWDRKLAGVFAFSSFLNYNSAVYDELKNNTGVTYPPLLQIHGNQDD 74
GFS GGAL + + + + G FS ++ +NS++ ++ + T P+L HG D
Sbjct: 389 GFSQGGALTLASVLLYPKTIGGGAVFSGWIPFNSSITNQFTEDAKKT--PILWSHGIDDK 446
Query: 75 LVPLKWGEETYQKLKSLGIQGSFFV 99
V + G+ L+ G+ F V
Sbjct: 447 TVLFEAGQAALPFLQQAGVTCEFKV 471
>At3g15650.1 68416.m01984 phospholipase/carboxylesterase family
protein low similarity to lysophospholipase I [Mus
musculus] GI:1864159; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 255
Score = 41.1 bits (92), Expect = 2e-04
Identities = 32/125 (25%), Positives = 57/125 (45%), Gaps = 16/125 (12%)
Query: 15 GFSMGGALAFH-----------TGYRWDRKLAGVFAFSSFLNYNSAVYDELKNNTGVTYP 63
GFSMG A+A + TG+ + L S +L ++ +++++ V
Sbjct: 125 GFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVGLSGWLPGWRSLRSKIESSNEVARR 184
Query: 64 ----PLLQIHGNQDDLVPLKWGEETYQKLKSLGIQGSFF-VQERLGHSLNRRGIKIIKDW 118
P+L HG DD+VP ++GE++ L G + + F E LGH + + + W
Sbjct: 185 AASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQTMFKPYEGLGHYTVPKEMDEVVHW 244
Query: 119 IDEKL 123
+ +L
Sbjct: 245 LVSRL 249
>At1g52700.1 68414.m05952 phospholipase/carboxylesterase family
protein similar to lysophospholipase I [Mus musculus]
GI:1864159; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 255
Score = 40.3 bits (90), Expect = 4e-04
Identities = 33/125 (26%), Positives = 54/125 (43%), Gaps = 16/125 (12%)
Query: 15 GFSMGGALAFH-----------TGYRWDRKLAGVFAFSSFL----NYNSAVYDELKNNTG 59
GFSMG A++ + TG+ + L V S +L + S + +
Sbjct: 125 GFSMGAAISLYSATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSFEAARR 184
Query: 60 VTYPPLLQIHGNQDDLVPLKWGEETYQKLKSLGIQGSFF-VQERLGHSLNRRGIKIIKDW 118
P++ HG DD+VP ++GE++ Q L G + + F E LGH R + + W
Sbjct: 185 AASLPIILTHGTSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMDEVVHW 244
Query: 119 IDEKL 123
+ L
Sbjct: 245 LTTML 249
>At5g20060.2 68418.m02389 phospholipase/carboxylesterase family
protein similar to lysophospholipase II [Mus musculus]
GI:4589453; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 252
Score = 37.5 bits (83), Expect = 0.003
Identities = 22/73 (30%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
Query: 52 DELKNNTGVTYPPLLQIHGNQDDLVPLKWGEETYQKLKSLGIQG-SFFVQERLGHSLNRR 110
+++KN P++ HG DD+VP K+GE++ Q L S G + +F LGH +
Sbjct: 178 EQIKNRAASL--PIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQ 235
Query: 111 GIKIIKDWIDEKL 123
+ + W+ L
Sbjct: 236 ELDELCAWLTSTL 248
>At5g20060.1 68418.m02388 phospholipase/carboxylesterase family
protein similar to lysophospholipase II [Mus musculus]
GI:4589453; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 252
Score = 37.5 bits (83), Expect = 0.003
Identities = 22/73 (30%), Positives = 37/73 (50%), Gaps = 3/73 (4%)
Query: 52 DELKNNTGVTYPPLLQIHGNQDDLVPLKWGEETYQKLKSLGIQG-SFFVQERLGHSLNRR 110
+++KN P++ HG DD+VP K+GE++ Q L S G + +F LGH +
Sbjct: 178 EQIKNRAASL--PIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQ 235
Query: 111 GIKIIKDWIDEKL 123
+ + W+ L
Sbjct: 236 ELDELCAWLTSTL 248
>At1g62870.1 68414.m07099 expressed protein
Length = 796
Score = 31.1 bits (67), Expect = 0.23
Identities = 15/31 (48%), Positives = 20/31 (64%)
Query: 60 VTYPPLLQIHGNQDDLVPLKWGEETYQKLKS 90
VT P L + G +DDL PL E++ +KLKS
Sbjct: 176 VTQQPHLMLSGGKDDLGPLAMLEDSVKKLKS 206
>At1g52460.1 68414.m05922 hypothetical protein
Length = 131
Score = 29.9 bits (64), Expect = 0.54
Identities = 22/65 (33%), Positives = 33/65 (50%), Gaps = 7/65 (10%)
Query: 65 LLQIHGNQDDLVPL-KWGEETYQKLKSLGIQGSFFVQ-ERLG---HSLNRRGIKIIKDWI 119
+L HG +D + L GEE L+ G + F+ R G H +NR ++K W+
Sbjct: 58 ILLTHGQRDHVPHLCGCGEEAAFILREAGFRDVRFLPFARFGPIAHEINRN--VMVKSWL 115
Query: 120 DEKLP 124
+EKLP
Sbjct: 116 EEKLP 120
>At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1)
identical to RNA-dependent RNA polymerase [Arabidopsis
thaliana] gi|8248473|gb|AAF74208
Length = 1196
Score = 29.5 bits (63), Expect = 0.71
Identities = 14/50 (28%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Query: 52 DELKNNTGVTYPPLLQIHGNQDDLVPLKWGEETYQKLKSLGIQGSFFVQE 101
+E + + + Y +L+I G +D L+P WG + + +G+ G + VQ+
Sbjct: 1012 EESTDPSAIPYDAVLEIPGFED-LIPEAWGHKCLYDGQLIGLLGQYKVQK 1060
>At2g24320.1 68415.m02907 hypothetical protein
Length = 286
Score = 28.3 bits (60), Expect = 1.6
Identities = 24/85 (28%), Positives = 38/85 (44%), Gaps = 11/85 (12%)
Query: 15 GFSMGGALAFHTGYRWDRKLAGVFAFSSFLNYNSAVY--------DELKNNTGVTYP--P 64
G S+G H R R L G+ S+ L+ +Y D KN + + P
Sbjct: 137 GQSVGSGPTLHLASRVKR-LRGIVLHSAILSGLRVLYPVKMTFWFDMYKNIDKIRHVTCP 195
Query: 65 LLQIHGNQDDLVPLKWGEETYQKLK 89
+L IHG +DD+V + G+ ++ K
Sbjct: 196 VLVIHGTKDDIVNMSHGKRLWELAK 220
>At1g12380.1 68414.m01431 expressed protein
Length = 793
Score = 27.9 bits (59), Expect = 2.2
Identities = 13/28 (46%), Positives = 18/28 (64%)
Query: 63 PPLLQIHGNQDDLVPLKWGEETYQKLKS 90
P L + G +DDL PL E++ +KLKS
Sbjct: 192 PQHLMLSGGKDDLGPLAMLEDSVKKLKS 219
>At1g31920.1 68414.m03922 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 606
Score = 27.5 bits (58), Expect = 2.9
Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 3/92 (3%)
Query: 32 RKLAGVFAFSSFLNYNSAVYDELKNNTGVTYPPLLQIHGNQDDLVPLKWGEETYQKLKSL 91
R V +F L + + + TYP LL+ L ++ G++ + ++ L
Sbjct: 105 RGYVNVMSFEEALCFYNEMMQRGNEPDNFTYPCLLKACTR---LKSIREGKQIHGQVFKL 161
Query: 92 GIQGSFFVQERLGHSLNRRGIKIIKDWIDEKL 123
G++ FVQ L + R G + + EKL
Sbjct: 162 GLEADVFVQNSLINMYGRCGEMELSSAVFEKL 193
>At1g51860.1 68414.m05846 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 890
Score = 27.1 bits (57), Expect = 3.8
Identities = 17/60 (28%), Positives = 29/60 (48%)
Query: 36 GVFAFSSFLNYNSAVYDELKNNTGVTYPPLLQIHGNQDDLVPLKWGEETYQKLKSLGIQG 95
G+F F+ + NS + L T +LQ+ N+D++ + +ETY K + QG
Sbjct: 329 GIFNFTFAMTGNSTLPPLLNALEIYTVVDILQLETNKDEVSAMMNIKETYGLSKKISWQG 388
>At1g09320.1 68414.m01043 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 517
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Query: 92 GIQGSFFVQERLGHSLNRRGIKIIKDWID 120
G G +F Q + R+G+++ KDW+D
Sbjct: 317 GTVGIYFRQSQEKMRFGRQGLRLHKDWVD 345
>At4g33180.1 68417.m04726 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas fluorescens] GI:1871461; contains Pfam
profile PF00561: alpha/beta hydrolase fold
Length = 307
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/32 (37%), Positives = 17/32 (53%)
Query: 14 SGFSMGGALAFHTGYRWDRKLAGVFAFSSFLN 45
+G S GG +A+H W K+ V SS +N
Sbjct: 128 AGTSYGGFVAYHMAKMWPEKVEKVVIASSGIN 159
>At3g30380.1 68416.m03835 expressed protein ; expression supported
by MPSS
Length = 399
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
Query: 51 YDELKNNTGVTYP--PLLQIHGNQDDLVPLKWGEETYQKLK 89
+D KN +++ P+L IHG DD+V G++ ++ K
Sbjct: 187 FDIYKNVEKISFVKCPVLVIHGTSDDVVNWSHGKQLFELCK 227
>At3g14730.1 68416.m01862 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 653
Score = 26.6 bits (56), Expect = 5.0
Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 4/55 (7%)
Query: 4 LLQGSNVVEKSGFSMGGALAFHTGYRWDRKLAG--VFAFSSFLNYNSA--VYDEL 54
LL+GS+ +E S LAF G+ D + V ++S F++ A V+DEL
Sbjct: 167 LLKGSDAMELSDVKKVHGLAFKLGFDSDCYVGSGLVTSYSKFMSVEDAQKVFDEL 221
>At5g66470.1 68418.m08382 expressed protein
Length = 427
Score = 26.2 bits (55), Expect = 6.6
Identities = 17/62 (27%), Positives = 29/62 (46%), Gaps = 5/62 (8%)
Query: 63 PPLLQIHGNQDDLVPLKWGEETYQKLKSLGIQGSFFVQERLGHSLNRRGIKIIKDWIDEK 122
PP+L + +D + P + ++ K + V + GH GI+ +K+WI K
Sbjct: 241 PPMLLVMNKKDLIKPGEIAKKLEWYEKFTDVDEVIPVSAKYGH-----GIEDVKEWILSK 295
Query: 123 LP 124
LP
Sbjct: 296 LP 297
>At4g24760.1 68417.m03545 expressed protein
Length = 365
Score = 26.2 bits (55), Expect = 6.6
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Query: 51 YDELKNNTGVTYP--PLLQIHGNQDDLVPLKWGEETYQ 86
+D KN +T P+L IHG DD+V G++ ++
Sbjct: 188 FDIYKNIDKITLVRCPVLVIHGTADDVVDFSHGKQLWE 225
>At4g11050.1 68417.m01796 endo-1,4-beta-glucanase, putative /
cellulase, putative similar to endo-beta-1,4-glucanase
GI:4972236 from [Fragaria x ananassa]
Length = 626
Score = 26.2 bits (55), Expect = 6.6
Identities = 13/25 (52%), Positives = 15/25 (60%)
Query: 13 KSGFSMGGALAFHTGYRWDRKLAGV 37
K+G SMGG T + WD K AGV
Sbjct: 260 KNGDSMGGTGWSMTEFGWDVKYAGV 284
>At2g04300.1 68415.m00422 leucine-rich repeat protein kinase,
putative similar to light repressible receptor protein
kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376;
contains leucine rich repeat (LRR) domains,
Pfam:PF00560; contains protein kinase domain,
Pfam:PF00069
Length = 851
Score = 26.2 bits (55), Expect = 6.6
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
Query: 40 FSSFLNYNSAVYDELKNNTGVTYPPLLQIHGNQDDLVPLKWGEETY 85
F LN N A+ L+ T + +P ++ NQDD++ +K + TY
Sbjct: 299 FDMMLNGNLALERALEVFTVIDFP---ELETNQDDVIAIKNIQNTY 341
>At1g44170.2 68414.m05102 aldehyde dehydrogenase, putative (ALDH)
similar to aldehyde dehydrogenase ALDH [Craterostigma
plantagineum] gi|17065918|emb|CAC84900
Length = 484
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/29 (34%), Positives = 17/29 (58%)
Query: 36 GVFAFSSFLNYNSAVYDELKNNTGVTYPP 64
G F+F +F + + +Y L ++ V YPP
Sbjct: 428 GKFSFDAFSHKKAVLYRSLFGDSAVRYPP 456
>At1g44170.1 68414.m05101 aldehyde dehydrogenase, putative (ALDH)
similar to aldehyde dehydrogenase ALDH [Craterostigma
plantagineum] gi|17065918|emb|CAC84900
Length = 484
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/29 (34%), Positives = 17/29 (58%)
Query: 36 GVFAFSSFLNYNSAVYDELKNNTGVTYPP 64
G F+F +F + + +Y L ++ V YPP
Sbjct: 428 GKFSFDAFSHKKAVLYRSLFGDSAVRYPP 456
>At3g01690.1 68416.m00101 expressed protein
Length = 361
Score = 25.8 bits (54), Expect = 8.8
Identities = 13/41 (31%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Query: 51 YDELKNNTGVTYP--PLLQIHGNQDDLVPLKWGEETYQKLK 89
+D KN + Y P+L IHG D++V G++ ++ K
Sbjct: 188 FDIYKNIDKIPYVDCPVLIIHGTSDEVVDCSHGKQLWELCK 228
>At1g73200.1 68414.m08471 expressed protein
Length = 779
Score = 25.8 bits (54), Expect = 8.8
Identities = 13/36 (36%), Positives = 22/36 (61%), Gaps = 7/36 (19%)
Query: 96 SFFVQERLGHSLNRRGI-------KIIKDWIDEKLP 124
SF ++ + SLN++G+ + +KDW+ EKLP
Sbjct: 54 SFDPRQSIDFSLNKQGVIWILELDENVKDWMKEKLP 89
>At1g52695.1 68414.m05951 phospholipase/carboxylesterase family
protein contains Pfam profile: PF02230
phospholipase/carboxylesterase; supported by full length
cDNA gi:26450919 from [Arabidopsis thaliana]
Length = 231
Score = 25.8 bits (54), Expect = 8.8
Identities = 11/33 (33%), Positives = 16/33 (48%)
Query: 65 LLQIHGNQDDLVPLKWGEETYQKLKSLGIQGSF 97
+L +HG DD+VP +G L+ G F
Sbjct: 178 ILILHGTSDDVVPSSFGYRCADSLRMAGFPTLF 210
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 25.8 bits (54), Expect = 8.8
Identities = 22/97 (22%), Positives = 43/97 (44%), Gaps = 6/97 (6%)
Query: 29 RWDRKLAGVFAFSSFLNYNSAVYDELKNNTGVTYPPL--LQIHGNQDDLVPLKWGEETYQ 86
RW AGV + S Y S + L + +T + LQ G + ++ E + +
Sbjct: 545 RWHLVAAGVASVSLHQCYGSILMQYLAGRSTITKETVEVLQTAGKLEKVLVQMVAENSDE 604
Query: 87 KLKSLGIQGSFFVQERLGHSLNRRGIKIIKDWIDEKL 123
G V+E + + ++ +++++ WI+EKL
Sbjct: 605 CEDG----GKGLVREMVPYEVDSIILRLLRQWIEEKL 637
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.139 0.423
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,289,292
Number of Sequences: 28952
Number of extensions: 132072
Number of successful extensions: 348
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 330
Number of HSP's gapped (non-prelim): 27
length of query: 126
length of database: 12,070,560
effective HSP length: 73
effective length of query: 53
effective length of database: 9,957,064
effective search space: 527724392
effective search space used: 527724392
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 54 (25.8 bits)
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