Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000504-TA|BGIBMGA000504-PA|undefined
         (340 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g61250.1 68414.m06902 secretory carrier membrane protein (SCA...    32   0.63 
At2g31960.1 68415.m03905 glycosyl transferase family 48 protein ...    29   3.4  
At1g11180.1 68414.m01281 secretory carrier membrane protein (SCA...    29   3.4  
At1g05570.1 68414.m00575 callose synthase 1 (CALS1) / 1,3-beta-g...    29   3.4  
At2g47600.1 68415.m05939 magnesium/proton exchanger (MHX1) ident...    29   4.5  
At4g00370.1 68417.m00051 sugar transporter family protein contai...    29   5.9  
At1g33090.1 68414.m04085 MATE efflux family protein similar to r...    28   7.8  
At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note:...    28   7.8  

>At1g61250.1 68414.m06902 secretory carrier membrane protein (SCAMP)
           family protein (SC3) contains Pfam domain, PF04144:
           SCAMP family
          Length = 289

 Score = 31.9 bits (69), Expect = 0.63
 Identities = 25/104 (24%), Positives = 47/104 (45%), Gaps = 9/104 (8%)

Query: 201 WASAGKKVEWKTLILATILGMPGGVLLFVPIYHPLHDIYKIHSEIT---FFLLFAIYGTF 257
           W        W   ++  I G+PGG +L+   Y PL+  ++  S ++   FFL + ++  F
Sbjct: 149 WIKGEGVTIWLLALIYFIAGVPGGYVLW---YRPLYRAFRTDSALSFGWFFLFYMLHIAF 205

Query: 258 VIHGLLSDRE--KSKEKLTLIDYILILQ-LALHYVIYWIFVVFF 298
            +   ++     K K    ++  I +L   A+  + Y+I   FF
Sbjct: 206 CVFAAVAPPVVFKGKSLAGILPAIDVLSGQAIVGIFYFIGFAFF 249


>At2g31960.1 68415.m03905 glycosyl transferase family 48 protein
           contains Pfam profile: PF02364 1,3-beta-glucan synthase;
           contains non-consensus splice aite AC at exon 33
          Length = 1959

 Score = 29.5 bits (63), Expect = 3.4
 Identities = 20/76 (26%), Positives = 35/76 (46%), Gaps = 6/76 (7%)

Query: 85  WHSQTPIIFLGARLPLHIILLYPAFIYHAAYAVSKLNFPRYAEPFAVGLVTVLIDIPYDI 144
           W SQ P +++G  +    + L+   ++     +SKL F  YAE      +  L+    DI
Sbjct: 640 WWSQ-PRLYIGRGMHESALSLFKYTMFWVVLLISKLAFSFYAEQ-----IKPLVKPTKDI 693

Query: 145 VAVKFVHWTWHDTDPN 160
           + V    + WH+  P+
Sbjct: 694 MRVHISVYRWHEFFPH 709


>At1g11180.1 68414.m01281 secretory carrier membrane protein (SCAMP)
           family protein contains Pfam domain, PF04144: SCAMP
           family
          Length = 291

 Score = 29.5 bits (63), Expect = 3.4
 Identities = 16/64 (25%), Positives = 27/64 (42%), Gaps = 3/64 (4%)

Query: 201 WASAGKKVEWKTLILATILGMPGGVLLFVPIYHPLHDIYKIHSEITFFLLFAIYGTFVIH 260
           W        W   ++  I G+PGG +L+   Y PL+  ++  S   F   F  Y   ++ 
Sbjct: 151 WIKGEGVTIWLLAVIYFISGVPGGYVLW---YRPLYRAFRSDSAFNFGWFFLFYMLHILF 207

Query: 261 GLLS 264
            L +
Sbjct: 208 CLFA 211


>At1g05570.1 68414.m00575 callose synthase 1 (CALS1) /
           1,3-beta-glucan synthase 1 nearly identical to callose
           synthase 1 catalytic subunit [Arabidopsis thaliana]
           GI:13649388
          Length = 1922

 Score = 29.5 bits (63), Expect = 3.4
 Identities = 19/76 (25%), Positives = 36/76 (47%), Gaps = 7/76 (9%)

Query: 85  WHSQTPIIFLGARLPLHIILLYPAFIYHAAYAVSKLNFPRYAEPFAVGLVTVLIDIPYDI 144
           W SQ P +++G  +    + L+   ++     +SKL F  YAE      +  L+    DI
Sbjct: 612 WWSQ-PRLYIGRGMHESALSLFKYTMFWIVLLISKLAFSYYAE------IKPLVGPTKDI 664

Query: 145 VAVKFVHWTWHDTDPN 160
           + +    ++WH+  P+
Sbjct: 665 MRIHISVYSWHEFFPH 680


>At2g47600.1 68415.m05939 magnesium/proton exchanger (MHX1)
           identical to magnesium/proton exchanger AtMHX
           [Arabidopsis thaliana] gi|6492237|gb|AAF14229;
           Ca2+:Cation Antiporter (CaCA) Family member
           PMID:11500563
          Length = 539

 Score = 29.1 bits (62), Expect = 4.5
 Identities = 13/41 (31%), Positives = 21/41 (51%)

Query: 21  IKQLWAAQPTYIISQAVYVLAGLLTLFHAFKKGGRWPYFWL 61
           I ++W+     ++   + VL   L L HA+ +  RWPY  L
Sbjct: 187 ILEVWSPNVITLVEALLTVLQYGLLLVHAYAQDKRWPYLSL 227


>At4g00370.1 68417.m00051 sugar transporter family protein contains
           Pfam profile PF00083: major facilitator superfamily
           protein
          Length = 541

 Score = 28.7 bits (61), Expect = 5.9
 Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 4/57 (7%)

Query: 58  YFW---LGTVLHGIYADNFWHFVLPEYDNFWHSQTPIIF-LGARLPLHIILLYPAFI 110
           +FW   L  +L GI+AD F   V+  +   W S   I+  + ARL L  +L+  AF+
Sbjct: 177 FFWGYLLTQILGGIWADKFGGKVVLGFGVVWWSFATIMTPIAARLGLPFLLVVRAFM 233


>At1g33090.1 68414.m04085 MATE efflux family protein similar to
           ripening regulated protein DDTFR18 [Lycopersicon
           esculentum] GI:12231296; contains Pfam profile PF01554:
           Uncharacterized membrane protein family
          Length = 494

 Score = 28.3 bits (60), Expect = 7.8
 Identities = 17/50 (34%), Positives = 24/50 (48%)

Query: 250 LFAIYGTFVIHGLLSDREKSKEKLTLIDYILILQLALHYVIYWIFVVFFH 299
           L AI  TFV         +S+ K  +I Y+  + L LH    W+ VV F+
Sbjct: 163 LIAINFTFVPAFTCQIFLQSQSKNKIIAYVSAVTLGLHVFFSWLLVVHFN 212


>At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note:
           CHX6a and CHX6b were originally 1 gene but were split
           pased on alignments with other family members; may be a
           pseudogene and requires futher investigation; monovalent
           cation:proton antiporter family 2 (CPA2) member,
           PMID:11500563
          Length = 824

 Score = 28.3 bits (60), Expect = 7.8
 Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 2/70 (2%)

Query: 245 ITFFLLFAIYGTFVIHGLLSDREKSKEKLTLIDYILILQLALHYVIYWIFVVFFHPEREW 304
           + FF+   +   F++  +    E +  K   + YI +L   L Y +YW   +FF P   +
Sbjct: 290 MAFFIFVYLVRQFMLWVIRHTPEGAPVKNVYL-YIGLLLAYLSY-LYWSRFLFFGPLGAF 347

Query: 305 STGLHEPVGP 314
           + GL  P GP
Sbjct: 348 ALGLAVPNGP 357


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.327    0.143    0.493 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,302,994
Number of Sequences: 28952
Number of extensions: 348117
Number of successful extensions: 769
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 766
Number of HSP's gapped (non-prelim): 10
length of query: 340
length of database: 12,070,560
effective HSP length: 82
effective length of query: 258
effective length of database: 9,696,496
effective search space: 2501695968
effective search space used: 2501695968
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 60 (28.3 bits)