Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000484-TA|BGIBMGA000484-PA|IPR002553|Adaptin, N-terminal
         (753 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / ga...   510   e-144
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit...    39   0.011
At4g11380.1 68417.m01835 beta-adaptin, putative strong similarit...    39   0.014
At5g09790.1 68418.m01133 PHD finger family protein / SET domain-...    33   0.92 
At1g64110.2 68414.m07264 AAA-type ATPase family protein contains...    31   2.8  
At1g64110.1 68414.m07263 AAA-type ATPase family protein contains...    31   2.8  
At1g30410.1 68414.m03717 ATP-binding cassette transport protein,...    31   3.7  
At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar ...    30   4.9  
At1g60720.1 68414.m06835 hypothetical protein                          30   4.9  

>At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative /
           gamma-2 coat protein, putative / gamma-2 COP, putative
           similar to SP|Q9UBF2 Coatomer gamma-2 subunit (Gamma-2
           coat protein) (Gamma-2 COP) {Homo sapiens}; contains
           Pfam profile: PF01602 Adaptin N terminal region
          Length = 886

 Score =  510 bits (1258), Expect = e-144
 Identities = 300/767 (39%), Positives = 415/767 (54%), Gaps = 29/767 (3%)

Query: 1   MTGKDDEYRPAAIRALCSITDSTMLQAIERYMKQAIVDKNPXXXXXXXXXXXXXXXXXPD 60
           M  K D YR  AIR LC I D T+L  IERY+KQAIVDKNP                 P+
Sbjct: 115 MNSKIDMYRANAIRVLCRIIDGTLLTQIERYLKQAIVDKNPVVSSAALVSGLHLLKTNPE 174

Query: 61  LVRRWINEAQEAMTSDHVMVSYHALAVVAGARRNDRLSTVKLITKLARTPVRSPYTLCLQ 120
           +V+RW NE QE + S   +V +HALA++   R+NDRL+  KL+  L R  VRSP   CL 
Sbjct: 175 IVKRWSNEVQEGIQSRSALVQFHALALLHQIRQNDRLAVSKLVGSLTRGSVRSPLAQCLL 234

Query: 121 IRFAAKLAEE--DPSEASE-PYLEFIESCLRNKSEMVVYEAAHAIVNLRK-SAKDLAQAV 176
           IR+ +++  +  +  ++ E P+ EF+ESCLR+K+EMV+ EAA AI  L   ++++L  A+
Sbjct: 235 IRYTSQVIRDMANHGQSGERPFYEFLESCLRHKAEMVILEAARAITELDGVTSRELTPAI 294

Query: 177 SVLQIFCGSSKXXXXXXXXXXXXXXXXKHPNAVAACAVDLENLISDSNRSXXXXXXXXXX 236
           +VLQ+F  S +                 HP AV  C +D+E+LISD NRS          
Sbjct: 295 TVLQLFLSSPRPVLRFAAVRTLNKVAMTHPMAVTNCNIDMESLISDQNRSIATLAITTLL 354

Query: 237 XXGAESSIDRLMKQISEFMSEISDEFKIVVVRAIRRLCSKYPRKHQSLAAFLAGMLRDEG 296
             G ESS++RLMKQI+ FMS+I+DEFKIVVV AIR LC K+P K++SL  FL+ +LR+EG
Sbjct: 355 KTGNESSVERLMKQITNFMSDIADEFKIVVVDAIRSLCVKFPLKYRSLMTFLSNILREEG 414

Query: 297 GLQYKTXXXXXXXXXXXXNPDAKETGLAHLCEFIEDCEHVTLAVRILHVLGREGPKARQP 356
           G +YK              PDAKE+GL HLCEFIEDCE   L+ +ILH LG EGP    P
Sbjct: 415 GFEYKRAIVDSIVTIIRDIPDAKESGLLHLCEFIEDCEFTYLSTQILHFLGIEGPNTSDP 474

Query: 357 SRYIRYIYNRVILEXXXXXXXXXXXXXXXXXXXPELLPNIRVLLSRCQLDEDDEVRDRAV 416
           S+YIRYIYNRV LE                     L P I VLL RC  D DDEVRDRA 
Sbjct: 475 SKYIRYIYNRVHLENATVRAAAVSTLAKFGFMVESLKPRITVLLKRCIYDSDDEVRDRAT 534

Query: 417 FYSAILDS----GNPQLINDYII-NIQVPNPVLLEKSLSDYLASGD----QSEPFNIAAV 467
            Y ++L         +   D++  +++VP  V +E SL +Y  S +     S P  + + 
Sbjct: 535 LYLSVLGGDGTVDTDKESKDFLFGSLEVP-LVNMETSLKNYEPSEEAFDINSVPKEVKSQ 593

Query: 468 PTAEEPQEPKESPVEIETRKAPVVSREEQYTEQLQAIPGIEKLGPLFKSCEAIDLTEPET 527
           P AE+  + K+ P  +    A   S  + Y   L +IP     G LFKS   ++LTE ET
Sbjct: 594 PLAEKKAQGKK-PTGLGAPPAAPASGFDGYERLLSSIPEFAAFGKLFKSSLPVELTEAET 652

Query: 528 EYRVRCVKHIFARHLILQFECLNTLSDQLLEKVYVRLDSIPGYKISNVV--PCDELPYDK 585
           EY V  VKHIF  H++ Q+ C NT+ +QLLE+V V +D+    + S V     + LPYD 
Sbjct: 653 EYAVNVVKHIFDSHVVFQYNCTNTIPEQLLERVNVIVDASEAEEFSEVTSKALNSLPYDS 712

Query: 586 QGNIFCLLEFPENPIETLGNFGATLEFVVRDCDPTTGLPDSNEGYADTYPLEEIEITCAD 645
            G  F + E P   +  +G F  TL FVV++ DP+TG  + ++G  D Y LE++E+   D
Sbjct: 713 PGQAFVVFEKPAG-VPAVGKFSNTLTFVVKEVDPSTGEAE-DDGVEDEYQLEDLEVVAGD 770

Query: 646 QFRTRAATDDWXXXXXXXXXXXXXCDTFGLTQSD-VGAAASAVCEHLGMPKAAISGEAVK 704
            +  +    ++              D +GL Q + +G A  AV + LGM     +     
Sbjct: 771 -YMVKVGVSNFRNAWESMDEEDERVDEYGLGQRESLGEAVKAVMDLLGMQTCEGTETIPL 829

Query: 705 EIRG-----GGLWRAGAPVLMRARL-VAAQGTVTMQLIARSPREDVA 745
             R       G++     VL+RA+  + +   + M+L  R+  EDV+
Sbjct: 830 NARSHTCLLSGVYIGNVKVLVRAQFGMDSSKDIAMKLTVRA--EDVS 874


>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
           to SP|Q10567 Adapter-related protein complex 1 beta 1
           subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
           [Drosophila melanogaster] GI:434902; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 893

 Score = 39.1 bits (87), Expect = 0.011
 Identities = 39/212 (18%), Positives = 84/212 (39%), Gaps = 8/212 (3%)

Query: 240 AESSIDRLMKQISEFMSEISDEFKIVVVRAIRRLCSKYPRKHQSLAAFLAGMLRDEGGLQ 299
           ++ +ID+++ +  E+ +E+  +F    VRAI R   K  R  +   + L  +++ +    
Sbjct: 347 SDRNIDQVLLEFKEYATEVDVDFVRKAVRAIGRCAIKLERAAERCISVLLELIKIKVNYV 406

Query: 300 YKTXXXXXXXXXXXXNPDAKETGLAHLCEFIEDCEHVTLAVRILHVLGREGPKARQPSRY 359
            +              P+  E+ +A LCE ++  +       ++ ++G    +       
Sbjct: 407 VQEAIIVIKDIFRRY-PNTYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDNADEL 465

Query: 360 IRYIYNRVILEXXXXXXXXXXXXXXXXXXXPELLPN--IRVLLSRCQLDEDD-EVRDRAV 416
           +         E                   P   P   I+V+L+   ++ D+ ++RDRA 
Sbjct: 466 LESFLENFPEEPAQVQLQLLTATVKLFLKKPTEGPQQMIQVVLNNATVETDNPDLRDRAY 525

Query: 417 FYSAILDSGNPQLINDYIINIQVPNPVLLEKS 448
            Y  +L S +P+   D ++      PV+ + S
Sbjct: 526 IYWRLL-STDPEAAKDVVL---AEKPVITDDS 553


>At4g11380.1 68417.m01835 beta-adaptin, putative strong similarity
           to SP|Q10567 Adapter-related protein complex 1 beta 1
           subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
           [Drosophila melanogaster] GI:434902; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 894

 Score = 38.7 bits (86), Expect = 0.014
 Identities = 36/199 (18%), Positives = 79/199 (39%), Gaps = 5/199 (2%)

Query: 240 AESSIDRLMKQISEFMSEISDEFKIVVVRAIRRLCSKYPRKHQSLAAFLAGMLRDEGGLQ 299
           ++ +ID+++ +  E+ +E+  +F    VRAI R   K  R  +   + L  +++ +    
Sbjct: 347 SDRNIDQVLLEFKEYATEVDVDFVRKAVRAIGRCAIKLERAAERCISVLLELIKIKVNYV 406

Query: 300 YKTXXXXXXXXXXXXNPDAKETGLAHLCEFIEDCEHVTLAVRILHVLGREGPKARQPSRY 359
            +              P+  E+ +A LCE ++  +       ++ ++G    +       
Sbjct: 407 VQEAIIVIKDIFRRY-PNTYESIIATLCESLDTLDEPEAKASMIWIIGEYAERIDNADEL 465

Query: 360 IRYIYNRVILEXXXXXXXXXXXXXXXXXXXPELLPN--IRVLLSRCQLDEDD-EVRDRAV 416
           +         E                   P   P   I+V+L+   ++ D+ ++RDRA 
Sbjct: 466 LESFLENFPEEPAQVQLQLLTATVKLFLKKPTEGPQQMIQVVLNNATVETDNPDLRDRAY 525

Query: 417 FYSAILDSGNPQLINDYII 435
            Y  +L S +P+   D ++
Sbjct: 526 IYWRLL-STDPEAAKDVVL 543


>At5g09790.1 68418.m01133 PHD finger family protein / SET
           domain-containing protein contains Pfam domain, PF00628:
           PHD-finger and PF00856: SET domain
          Length = 352

 Score = 32.7 bits (71), Expect = 0.92
 Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 2/89 (2%)

Query: 468 PTAEEPQEPK-ESPVEIETRKAPVVSREEQYTEQLQAIPGIEKLGPLFKSCEAIDLTEPE 526
           P++E P   K +S  EI  +  PVV +EE+  E   +    EK G      E +   + +
Sbjct: 27  PSSESPPPRKMKSMAEIMAKSVPVVEQEEEEDEDSYSNVTCEKCGSGEGDDELLLCDKCD 86

Query: 527 TEYRVRCVKHIFARHLILQFECLNTLSDQ 555
             + ++C++ I  R  I  + C++  SDQ
Sbjct: 87  RGFHMKCLRPIVVRVPIGTWLCVD-CSDQ 114


>At1g64110.2 68414.m07264 AAA-type ATPase family protein contains
           Pfam domain, PF00004: ATPase, AAA family
          Length = 829

 Score = 31.1 bits (67), Expect = 2.8
 Identities = 19/61 (31%), Positives = 35/61 (57%), Gaps = 5/61 (8%)

Query: 463 NIAAVPTAEEPQEPKESPVEIETRKAPVVSREEQYTEQL--QAIPGIEKLGPLFKSCEAI 520
           ++  V + EEP+  KE+  E  T KAP V+ + ++ +++  + IP  E++   FK   A+
Sbjct: 470 SVTTVSSKEEPE--KEAKAEKVTPKAPEVAPDNEFEKRIRPEVIPA-EEINVTFKDIGAL 526

Query: 521 D 521
           D
Sbjct: 527 D 527


>At1g64110.1 68414.m07263 AAA-type ATPase family protein contains
           Pfam domain, PF00004: ATPase, AAA family
          Length = 824

 Score = 31.1 bits (67), Expect = 2.8
 Identities = 19/61 (31%), Positives = 35/61 (57%), Gaps = 5/61 (8%)

Query: 463 NIAAVPTAEEPQEPKESPVEIETRKAPVVSREEQYTEQL--QAIPGIEKLGPLFKSCEAI 520
           ++  V + EEP+  KE+  E  T KAP V+ + ++ +++  + IP  E++   FK   A+
Sbjct: 465 SVTTVSSKEEPE--KEAKAEKVTPKAPEVAPDNEFEKRIRPEVIPA-EEINVTFKDIGAL 521

Query: 521 D 521
           D
Sbjct: 522 D 522


>At1g30410.1 68414.m03717 ATP-binding cassette transport protein,
           putative similar to MgATP-energized glutathione
           S-conjugate pump [Arabidopsis thaliana] GI:2909781;
           contains Pfam profiles PF00005: ABC transporter,
           PF00664: ABC transporter transmembrane region
          Length = 1495

 Score = 30.7 bits (66), Expect = 3.7
 Identities = 17/46 (36%), Positives = 20/46 (43%)

Query: 539 ARHLILQFECLNTLSDQLLEKVYVRLDSIPGYKISNVVPCDELPYD 584
           A +L +   C   L   LL      LD  PGY I N  P D + YD
Sbjct: 173 ALYLFISSRCSQALFGILLLIYIPELDPYPGYHIVNNEPLDNVEYD 218


>At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar to
           SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa
           Transmembrane trafficking protein) {Mesocricetus
           auratus}; contains Pfam profile: PF01105 emp24/gp25L/p24
           family; contains non-consensus TG acceptor splice site
           at exon 3
          Length = 217

 Score = 30.3 bits (65), Expect = 4.9
 Identities = 28/70 (40%), Positives = 38/70 (54%), Gaps = 7/70 (10%)

Query: 511 GPLFKSCEAIDLTEPETEYRVRCV-KHIFAR-HLILQFECLNTLSDQLLEKVYVRLDSIP 568
           G LF   EAI LT PE+    RCV + I A   ++L + C++    QL   + VR+ S  
Sbjct: 18  GSLFPGVEAIWLTVPES--GERCVYEEIQANVVVVLDYICIDDAFTQLGPTLDVRVTSPY 75

Query: 569 G---YKISNV 575
           G   YKI+NV
Sbjct: 76  GKELYKIANV 85


>At1g60720.1 68414.m06835 hypothetical protein
          Length = 289

 Score = 30.3 bits (65), Expect = 4.9
 Identities = 23/88 (26%), Positives = 38/88 (43%), Gaps = 2/88 (2%)

Query: 424 SGNPQLINDYIINIQVPNPVLLEKSLSDYLASGDQSEPFNIAAVPTAEEPQEPKESPVEI 483
           S   Q I+D+I  I  P+P  +E S  D++  G   + F+ A    A  P+ P+    + 
Sbjct: 72  SAPAQAIHDHISTITTPSPATIEDSF-DWVVGGVVCQGFSSARTWDAIRPRAPELDWAKA 130

Query: 484 ETRKAPVVSRE-EQYTEQLQAIPGIEKL 510
              K  V       +  QL  +P  ++L
Sbjct: 131 VWFKGAVPKHAFNMWISQLDRLPTRQRL 158


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.319    0.134    0.389 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,946,113
Number of Sequences: 28952
Number of extensions: 559695
Number of successful extensions: 1364
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1354
Number of HSP's gapped (non-prelim): 11
length of query: 753
length of database: 12,070,560
effective HSP length: 87
effective length of query: 666
effective length of database: 9,551,736
effective search space: 6361456176
effective search space used: 6361456176
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)