BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000475-TA|BGIBMGA000475- PA|IPR001580|Calreticulin/calnexin, IPR008985|Concanavalin A-like lectin/glucanase, IPR000886|Endoplasmic reticulum targeting sequence, IPR009169|Calreticulin (398 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 120 4e-28 SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 45 2e-05 SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 29 1.2 SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 6.1 SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 26 8.1 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 8.1 SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 8.1 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 120 bits (289), Expect = 4e-28 Identities = 91/273 (33%), Positives = 121/273 (44%), Gaps = 38/273 (13%) Query: 63 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 121 + GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + + Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147 Query: 122 MHGETPYEIMFGPDICGPGTKKVHVIFSYKG-------KNHLIKKDIRCKDDVYTHLYTL 174 M Y IMFGPD CG + VH IF +K + HL + T+LYTL Sbjct: 148 MSNSIDYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTL 206 Query: 175 IVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPE-----------------AKK 215 IVKPD T+EV I+ + V G L D+ LPP +I DPE A K Sbjct: 207 IVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADWVDEPEIPDPNAVK 266 Query: 216 PEDWXXXXXXXXXXXXXXX----XXXXXXHIPDPDATKXXXXXXXXXXXXXXXXIDNPDY 271 P+DW +IPDP+A K I NP Sbjct: 267 PDDWDEDAPRMIPDPDAVKPEDWLEDEPLYIPDPEAQKPEDWDDEEDGDWIPSEIINPKC 326 Query: 272 K-----GVWAPKQIDNPAYKGPWVHPEIDNPEY 299 G W P I NP Y+GPW P I NPE+ Sbjct: 327 IEGAGCGEWKPPMIRNPNYRGPWSPPMIPNPEF 359 Score = 54.8 bits (126), Expect = 2e-08 Identities = 26/92 (28%), Positives = 45/92 (48%), Gaps = 1/92 (1%) Query: 266 IDNPDYKGVWAPKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQVKSGT 325 I NP+Y+G W+P I NP + G W +I NP+Y D + + VG +LW ++ Sbjct: 340 IRNPNYRGPWSPPMIPNPEFIGEWYPRKIPNPDYFDDDHPSHFGPLYGVGFELWTMQPNI 399 Query: 326 IFDDFLITDDPAAAKERG-EVIKKRQEGEKKM 356 F + + A+ G E + + E+++ Sbjct: 400 RFSNIYVGHSIEDAERLGNETFLPKLKAEREL 431 >SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 44.8 bits (101), Expect = 2e-05 Identities = 52/194 (26%), Positives = 82/194 (42%), Gaps = 13/194 (6%) Query: 23 FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 78 F E+F + +W S W K+ G + L S D+ GL T + + + L S Sbjct: 33 FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89 Query: 79 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 136 P + + P+V+ F VK + CG Y+ + + K++ E P I FG Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148 Query: 137 CGPGTKKVHVIFSYKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 194 CG I SY GK HL + T +YTL+++ + T + D V +G Sbjct: 149 CGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ-NTTVVIRRDQSVVYTG 207 Query: 195 DLEADWDFLPPKKI 208 D+ ++ P K I Sbjct: 208 DVGTNFFHSPTKWI 221 >SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizosaccharomyces pombe|chr 2|||Manual Length = 551 Score = 29.1 bits (62), Expect = 1.2 Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 2/71 (2%) Query: 149 SYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLE-ADWDFLPPKK 207 S + K H KK +D Y+H+Y + P + + + ++ ES E + D+LP Sbjct: 137 STRKKEHFNKKPSFPRDTEYSHIYNMKYDPRSGIGIRVKRQEPESLKKENNNSDYLPKSA 196 Query: 208 IKDPEAKKPED 218 +K + P+D Sbjct: 197 MKQKKG-GPKD 206 >SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 26.6 bits (56), Expect = 6.1 Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 6/71 (8%) Query: 106 GGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 161 G +LKV + E M ETP +I++ + G+ +H K + KK++ Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673 Query: 162 R--CKDDVYTH 170 CK+D + + Sbjct: 674 LLFCKEDEFLY 684 >SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharomyces pombe|chr 1|||Manual Length = 343 Score = 26.2 bits (55), Expect = 8.1 Identities = 17/69 (24%), Positives = 33/69 (47%), Gaps = 9/69 (13%) Query: 110 LKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYT 169 +K+F + +K + +P+E+M P + P K H + ++R +DV T Sbjct: 154 VKMFTQIIIEKYFNPASPHEVMLPPQLVQP---------ILDCKEHQRQDELRLFEDVET 204 Query: 170 HLYTLIVKP 178 +L ++KP Sbjct: 205 YLLNFLLKP 213 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 26.2 bits (55), Expect = 8.1 Identities = 12/48 (25%), Positives = 24/48 (50%), Gaps = 1/48 (2%) Query: 25 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 72 + F +D + +W EH K ++ + + FS+ DD+ + SE + Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95 >SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 26.2 bits (55), Expect = 8.1 Identities = 12/28 (42%), Positives = 15/28 (53%) Query: 192 ESGDLEADWDFLPPKKIKDPEAKKPEDW 219 E L+ LP + K P AKKPED+ Sbjct: 157 EKNTLDLKHCLLPSPEYKAPPAKKPEDY 184 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.139 0.440 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,785,361 Number of Sequences: 5004 Number of extensions: 78833 Number of successful extensions: 132 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 120 Number of HSP's gapped (non-prelim): 9 length of query: 398 length of database: 2,362,478 effective HSP length: 74 effective length of query: 324 effective length of database: 1,992,182 effective search space: 645466968 effective search space used: 645466968 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits) S2: 55 (26.2 bits)
- SilkBase 1999-2023 -