Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000475-TA|BGIBMGA000475-
PA|IPR001580|Calreticulin/calnexin, IPR008985|Concanavalin A-like
lectin/glucanase, IPR000886|Endoplasmic reticulum targeting sequence,
IPR009169|Calreticulin
         (398 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)             238   6e-63
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)               113   2e-25
SB_36074| Best HMM Match : DUF885 (HMM E-Value=0.23)                   30   2.9  
SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)                   29   5.0  
SB_10791| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   6.6  
SB_27442| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   8.7  
SB_50073| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   8.7  
SB_43224| Best HMM Match : Neur_chan_LBD (HMM E-Value=0.002)           29   8.7  

>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1137

 Score =  238 bits (582), Expect = 6e-63
 Identities = 119/216 (55%), Positives = 135/216 (62%), Gaps = 1/216 (0%)

Query: 142  KKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWD 201
            K + + FS K + ++       KDD  THLYTLIV+PDNTYEV IDNEKVESG+LE DWD
Sbjct: 825  KTLVIQFSVKHEQNIDCGGGYAKDDEMTHLYTLIVRPDNTYEVKIDNEKVESGELEKDWD 884

Query: 202  FLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXXXXHIPDPDATKXXXXXXXXXXXX 261
            FLPPK IKDPEAKKPEDW                     +IPDPDA K            
Sbjct: 885  FLPPKTIKDPEAKKPEDWDDKKMIDDPEDKKPEDWDKPQNIPDPDAKKPEDWDDEIDGEW 944

Query: 262  XXXXIDNPDYKGVWAPKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQV 321
                IDNP+YKG W PKQIDNP YKG WVHPEIDNPEY  D  LY   +I A+G DLWQV
Sbjct: 945  EPPMIDNPEYKGEWKPKQIDNPNYKGEWVHPEIDNPEYKADDKLYMYKDIGAIGFDLWQV 1004

Query: 322  KSGTIFDDFLITDDPAAAKE-RGEVIKKRQEGEKKM 356
            KSGTIFD+ L+TD    A++   E   K +EGEKKM
Sbjct: 1005 KSGTIFDNVLVTDSVEHAEQFAKETFDKTKEGEKKM 1040



 Score =  142 bits (343), Expect = 6e-34
 Identities = 85/200 (42%), Positives = 113/200 (56%), Gaps = 20/200 (10%)

Query: 21  VFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 80
           V F EKF D SWE  WV S   G + GKFK TAGKF+ D E DKG++TSEDA+FY +S K
Sbjct: 756 VHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGISAK 815

Query: 81  F-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGP 139
           F KPF+NEGK LV+QF+VKHEQ+IDCGGGY K      + +  H    Y ++  PD    
Sbjct: 816 FEKPFTNEGKTLVIQFSVKHEQNIDCGGGYAK------DDEMTH---LYTLIVRPD---- 862

Query: 140 GTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEAD 199
            T +V +       N  ++     KD  +    T+        E   D + ++  + +  
Sbjct: 863 NTYEVKI------DNEKVESGELEKDWDFLPPKTIKDPEAKKPEDWDDKKMIDDPEDKKP 916

Query: 200 WDFLPPKKIKDPEAKKPEDW 219
            D+  P+ I DP+AKKPEDW
Sbjct: 917 EDWDKPQNIPDPDAKKPEDW 936


>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
          Length = 1086

 Score =  113 bits (273), Expect = 2e-25
 Identities = 86/251 (34%), Positives = 112/251 (44%), Gaps = 27/251 (10%)

Query: 115 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKKDIRCK--------- 164
           C L Q+    +TPY IMFGPD CG   +K+H IF +K  KN  I++    K         
Sbjct: 28  CHLFQESFGDKTPYTIMFGPDKCGED-RKLHFIFRHKNPKNGTIEEKHAKKPTGNYNSVF 86

Query: 165 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPEAKKPEDWXXX 222
           D   THL+TL+V+PDNT+EV ID E V  G L  D      PP +I+DP+ K+PEDW   
Sbjct: 87  DGKKTHLFTLVVRPDNTFEVFIDQESVNKGSLLEDMTPPVNPPAEIEDPDDKRPEDWDER 146

Query: 223 XX--XXXXXXXXXXXXXXXXHIPDPDATKXXXXXXXXXXXXXXXXIDNP-----DYKGVW 275
                                IPD +A K                   P     +  G W
Sbjct: 147 EKIPDPDAEKPDDWDEDAPVKIPDTNAKKPEGWLDDEPEYVPDPNGIKPEDWDDEEDGEW 206

Query: 276 APKQIDNPAYK----GPWVHPEIDNPEYTPDSNLYKRDE---ICAVGLDLWQVKSGTIFD 328
              QI NP  +    G W  P  DNPEY    +    D       VGL+LW + S  +FD
Sbjct: 207 EAPQIANPKCEKVGCGEWKPPMADNPEYKGKWSAPLIDNPNYKVGVGLELWSMTSDILFD 266

Query: 329 DFLITDDPAAA 339
           + ++T+D A A
Sbjct: 267 NVIVTNDKAVA 277


>SB_36074| Best HMM Match : DUF885 (HMM E-Value=0.23)
          Length = 689

 Score = 30.3 bits (65), Expect = 2.9
 Identities = 15/48 (31%), Positives = 26/48 (54%), Gaps = 1/48 (2%)

Query: 275 WAPKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQVK 322
           W  KQ+   A++  W     +NP  + D+NLY+ + +   G+  WQV+
Sbjct: 640 WLDKQMSFIAFQEGWAL-YAENPILSHDTNLYEDNVLQRYGMLKWQVR 686


>SB_48987| Best HMM Match : Ank (HMM E-Value=2.7e-36)
          Length = 551

 Score = 29.5 bits (63), Expect = 5.0
 Identities = 16/42 (38%), Positives = 21/42 (50%)

Query: 160 DIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWD 201
           D+ CKDD         V  D T E L+D+ +V+S  L  D D
Sbjct: 485 DVSCKDDKNRTALHWAVGQDKTIEGLLDDPRVQSLQLVNDQD 526


>SB_10791| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 510

 Score = 29.1 bits (62), Expect = 6.6
 Identities = 12/31 (38%), Positives = 18/31 (58%)

Query: 187 DNEKVESGDLEADWDFLPPKKIKDPEAKKPE 217
           D++  + GD E +  FLPP    + E+K PE
Sbjct: 106 DSDNGDKGDSEDEESFLPPSIFDESESKGPE 136


>SB_27442| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 165

 Score = 28.7 bits (61), Expect = 8.7
 Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 1/32 (3%)

Query: 189 EKVESGDLEADWD-FLPPKKIKDPEAKKPEDW 219
           EKV +G++  ++D  LPP++ K PE+  PE +
Sbjct: 48  EKVWTGEVWREFDPLLPPRRQKGPESLDPETY 79


>SB_50073| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 48

 Score = 28.7 bits (61), Expect = 8.7
 Identities = 11/44 (25%), Positives = 24/44 (54%)

Query: 61  EDDKGLKTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDID 104
           +DD  L+  +D  F   ++  +P  ++  P+ V+F + + Q +D
Sbjct: 4   QDDNELRLIKDLFFRGYNKNVRPVEDKSMPVEVKFGIAYTQVVD 47


>SB_43224| Best HMM Match : Neur_chan_LBD (HMM E-Value=0.002)
          Length = 123

 Score = 28.7 bits (61), Expect = 8.7
 Identities = 11/44 (25%), Positives = 24/44 (54%)

Query: 61  EDDKGLKTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDID 104
           +DD  L+  +D  F   ++  +P  ++  P+ V+F + + Q +D
Sbjct: 27  QDDNELRLIKDLFFRGYNKNVRPVEDKSMPVEVKFGIAYTQVVD 70


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.316    0.139    0.440 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,094,155
Number of Sequences: 59808
Number of extensions: 595162
Number of successful extensions: 845
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 821
Number of HSP's gapped (non-prelim): 11
length of query: 398
length of database: 16,821,457
effective HSP length: 84
effective length of query: 314
effective length of database: 11,797,585
effective search space: 3704441690
effective search space used: 3704441690
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 61 (28.7 bits)