Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000475-TA|BGIBMGA000475-
PA|IPR001580|Calreticulin/calnexin, IPR008985|Concanavalin A-like
lectin/glucanase, IPR000886|Endoplasmic reticulum targeting sequence,
IPR009169|Calreticulin
(398 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 342 2e-94
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 342 3e-94
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 307 8e-84
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 137 1e-32
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 134 7e-32
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 127 1e-29
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 1.3
At3g31540.1 68416.m04025 hypothetical protein 31 1.8
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 2.4
At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like fami... 29 7.2
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 342 bits (841), Expect = 2e-94
Identities = 165/342 (48%), Positives = 215/342 (62%), Gaps = 6/342 (1%)
Query: 17 INCDVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 74
++ +V FEEKF +D WE WV S+ + G++K TAG + D +DKG++TSED RF
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Query: 75 YALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGP 134
YA+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGP
Sbjct: 78 YAISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGP 137
Query: 135 DICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 194
DICG TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD TY +LIDN + ++G
Sbjct: 138 DICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILIDNVEKQTG 197
Query: 195 DLEADWDFLPPKKIKDPEAKKPEDWXXXXXX-XXXXXXXXXXXXXXXHIPDPDATKXXXX 253
L +DWD LP KKIKDP AKKPEDW IPD DA K
Sbjct: 198 SLYSDWDLLPAKKIKDPSAKKPEDWDDKEYIPDPEDTKPAGYDDIPKEIPDTDAKKPEDW 257
Query: 254 XXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICA 313
I NP+Y G W PK+I NPAYKG W P IDNPE+ D LY ++
Sbjct: 258 DDEEDGEWTAPTIPNPEYNGEWKPKKIKNPAYKGKWKAPMIDNPEFKDDPELYVFPKLKY 317
Query: 314 VGLDLWQVKSGTIFDDFLITDDPAAAKERG-EVIKKRQEGEK 354
VG++LWQVKSG++FD+ L++DDP AK+ E K ++ EK
Sbjct: 318 VGVELWQVKSGSLFDNVLVSDDPEYAKKLAEETWGKHKDAEK 359
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 342 bits (840), Expect = 3e-94
Identities = 169/338 (50%), Positives = 212/338 (62%), Gaps = 6/338 (1%)
Query: 21 VFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 78
V FEE+F DD WE+ WV SE + G++K TAG + D +DKG++TSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAIS 81
Query: 79 RKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICG 138
+F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPDICG
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICG 141
Query: 139 PGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEA 198
TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD TY +LIDN + ++G L +
Sbjct: 142 YSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILIDNVEKQTGSLYS 201
Query: 199 DWDFLPPKKIKDPEAKKPEDWXXXXXXXX-XXXXXXXXXXXXXHIPDPDATKXXXXXXXX 257
DWD LPPKKIKDP AKKPEDW IPD D+ K
Sbjct: 202 DWDLLPPKKIKDPSAKKPEDWDEQEYISDPEDKKPDGYDDIPKEIPDTDSKKPEDWDDEE 261
Query: 258 XXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGLD 317
I NP+Y G W PKQI NP YKG W P IDNP++ D LY ++ VGL+
Sbjct: 262 DGEWTAPTIPNPEYMGEWKPKQIKNPNYKGKWEAPLIDNPDFKDDPELYVFPKLKYVGLE 321
Query: 318 LWQVKSGTIFDDFLITDDPAAAKE-RGEVIKKRQEGEK 354
LWQVKSG++FD+ LI DDP AK+ E K ++ EK
Sbjct: 322 LWQVKSGSLFDNVLICDDPDYAKKLADETWGKLKDAEK 359
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 307 bits (754), Expect = 8e-84
Identities = 153/341 (44%), Positives = 206/341 (60%), Gaps = 6/341 (1%)
Query: 20 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 77
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 78 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDIC 137
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPDIC
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDIC 146
Query: 138 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLE 197
G TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD +Y VL+DN++ E G +
Sbjct: 147 GTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKEREFGSMY 206
Query: 198 ADWDFLPPKKIKDPEAKKPEDWXXXXXXXX-XXXXXXXXXXXXXHIPDPDATKXXXXXXX 256
DWD LPP+KIK AKKPEDW IPD A +
Sbjct: 207 TDWDILPPRKIKVKNAKKPEDWDDREYIDDPNDVKPEGFDSIPREIPDRKAKEPEDWDEE 266
Query: 257 XXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGL 316
I N YKG W K+I NP YKG W +P IDNPE+ D +LY I G+
Sbjct: 267 ENGLWEPPKIPNSAYKGPWKAKRIKNPNYKGKWKNPWIDNPEFEDDPDLYVLKSIKYAGI 326
Query: 317 DLWQVKSGTIFDDFLITDDPAAAKE-RGEVIKKRQEGEKKM 356
++WQVK+G+IFD+ LI DDPA A+ + + +E EK++
Sbjct: 327 EVWQVKAGSIFDNILICDDPAYARSIVDDYFAQHRESEKEL 367
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 137 bits (332), Expect = 1e-32
Identities = 112/388 (28%), Positives = 173/388 (44%), Gaps = 67/388 (17%)
Query: 29 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF-KPFSNE 87
D+ ++ W+ S++ E G +K S+ +D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 88 GKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGTKKV 144
+V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG GT KV
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG-GTNKV 149
Query: 145 HVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEA 198
H I +K H +K D +H+YT I+KPDN +L+D E+ + +L +
Sbjct: 150 HFILKHKNPKSGEYVEHHLKFPPSVPYDKLSHVYTAILKPDNEVRILVDGEEKKKANLLS 209
Query: 199 DWDF----LPPKKIKDPEAKKPEDWXXXXX--XXXXXXXXXXXXXXXXHIPDPDATKXXX 252
DF +P K I DPE KKPEDW I D +A K
Sbjct: 210 GEDFEPALIPAKTIPDPEDKKPEDWDERAKIPDPNAVKPEDWDEDAPMEIEDEEAEKPEG 269
Query: 253 XXXXXXXXXXXXXIDNP-----DYKGVWAPKQIDNP-------------------AYKGP 288
P + G+W +IDNP AYKG
Sbjct: 270 WLDDEPEEVDDPEATKPEDWDDEEDGMWEAPKIDNPKCEAAPGCGEWKRPMKRNPAYKGK 329
Query: 289 WVHPEIDNPEY--------TPDSNLYKRDE-----ICAVGLDLWQVKSGTIFDDFLITDD 335
W P IDNP Y P+ + ++ D I A+G+++W ++ G +FD+ LI D
Sbjct: 330 WSSPLIDNPAYKGIWKPRDIPNPDYFELDRPDYEPIAAIGIEIWTMQDGILFDNILIAKD 389
Query: 336 PAAA--------KERGEVIKKRQEGEKK 355
A K + +V K++Q+ E++
Sbjct: 390 EKVAETYRQTTWKPKFDVEKEKQKAEEE 417
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 134 bits (325), Expect = 7e-32
Identities = 71/180 (39%), Positives = 93/180 (51%), Gaps = 2/180 (1%)
Query: 179 DNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPEAKKPEDWXXXXXXXXXXXXXXXXXXX 238
D Y VL+DN++ E G + DWD LPP+KIK AKKPEDW
Sbjct: 134 DTPYSVLVDNKEREFGSMYTDWDILPPRKIKVKNAKKPEDWDDREYIDDPNDVKPEGFDS 193
Query: 239 X-XHIPDPDATKXXXXXXXXXXXXXXXXIDNPDYKGVWAPKQIDNPAYKGPWVHPEIDNP 297
IPD A + I N YKG W K+I NP YKG W +P IDNP
Sbjct: 194 IPREIPDRKAKEPEDWDEEENGLWEPPKIPNSAYKGPWKAKRIKNPNYKGKWKNPWIDNP 253
Query: 298 EYTPDSNLYKRDEICAVGLDLWQVKSGTIFDDFLITDDPAAAKE-RGEVIKKRQEGEKKM 356
E+ D +LY I G+++WQVK+G+IFD+ LI DDPA A+ + + +E EK++
Sbjct: 254 EFEDDPDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARSIVDDYFAQHRESEKEL 313
Score = 109 bits (263), Expect = 2e-24
Identities = 72/203 (35%), Positives = 110/203 (54%), Gaps = 25/203 (12%)
Query: 20 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 77
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 78 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDIC 137
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVLV----- 141
Query: 138 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVES-GDL 196
K+ Y + L + I+ K+ KP++ D E ++ D+
Sbjct: 142 --DNKEREFGSMYTDWDILPPRKIKVKN---------AKKPED----WDDREYIDDPNDV 186
Query: 197 EADWDFLPPKKIKDPEAKKPEDW 219
+ + P++I D +AK+PEDW
Sbjct: 187 KPEGFDSIPREIPDRKAKEPEDW 209
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 127 bits (307), Expect = 1e-29
Identities = 109/384 (28%), Positives = 167/384 (43%), Gaps = 64/384 (16%)
Query: 29 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF---KPFS 85
D+ ++ WV SE + G +K S+ DD GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEK----SEGHDDYGLLVSEKAKKYGIVKELDVDEPLN 90
Query: 86 -NEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGT 141
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-AT 148
Query: 142 KKVHVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGD 195
KVH I +K H +K D+ +H+YT ++K DN +L+D E+ + G+
Sbjct: 149 NKVHFILKHKNPKSGEFVEHHLKFPPSVPFDMLSHVYTAVLKSDNEVRILVDGEEKKKGN 208
Query: 196 LEADWDFLPP---------------------KKIKDPEAKKPEDWXXXXXXXXXXXXXXX 234
L + DF PP KI DP A KP+DW
Sbjct: 209 LLSAEDFEPPLIPSKTIPDPEDKKPEDWDERAKIPDPNAVKPDDWDEDAPMEIEDEEAEK 268
Query: 235 ----XXXXXXHIPDPDATKXXXXXXXXXXXXXXXXIDNPDYK-----GVWAPKQIDNPAY 285
+ DP+A+K + N + G W NPAY
Sbjct: 269 PEGWLDDEPVEVEDPEASKPEDWDDEEDGEWEAPKVSNTKCEAAPGCGEWKRPMKRNPAY 328
Query: 286 KGPWVHPEIDNPEY----------TPDSNLYKR---DEICAVGLDLWQVKSGTIFDDFLI 332
KG W P IDNP Y PD +R + I A+G+++W ++ G +FD+ LI
Sbjct: 329 KGKWSSPLIDNPAYKGIWKPRDIPNPDYFELERPNLEPIAAIGIEIWTMQDGILFDNILI 388
Query: 333 T-DDPAAAKERGEVIKKRQEGEKK 355
+ D+ A R K + + EK+
Sbjct: 389 SKDEKVAETYRQSTWKPKFDVEKE 412
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 1.3
Identities = 12/38 (31%), Positives = 19/38 (50%)
Query: 93 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 130
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At3g31540.1 68416.m04025 hypothetical protein
Length = 699
Score = 30.7 bits (66), Expect = 1.8
Identities = 17/53 (32%), Positives = 23/53 (43%)
Query: 153 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPP 205
KN I + CKDD YT + + D E L+ + G L+ FL P
Sbjct: 252 KNMKIIEGFPCKDDAYTDYFFFVALEDAVLEDLVGKVLTKWGILDRPIRFLEP 304
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 2.4
Identities = 12/37 (32%), Positives = 18/37 (48%)
Query: 93 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 129
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like family
protein contains Pfam profile PF04091: Exocyst complex
subunit Sec15-like
Length = 789
Score = 28.7 bits (61), Expect = 7.2
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Query: 153 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEAD 199
KN KK+ +D TH IV D VL+D E+++S DL +D
Sbjct: 53 KNVARKKEAEIEDLCKTHYEEFIVAVDELRGVLVDAEELKS-DLASD 98
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.316 0.139 0.440
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,385,137
Number of Sequences: 28952
Number of extensions: 418746
Number of successful extensions: 575
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 520
Number of HSP's gapped (non-prelim): 20
length of query: 398
length of database: 12,070,560
effective HSP length: 83
effective length of query: 315
effective length of database: 9,667,544
effective search space: 3045276360
effective search space used: 3045276360
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 60 (28.3 bits)
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