Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000473-TA|BGIBMGA000473-PA|IPR004859|Putative 5-3
exonuclease
(1120 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 292 2e-79
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 235 3e-62
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 33 0.22
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 30 2.1
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 29 2.8
SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schi... 29 4.8
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|... 28 8.4
SPAC29A4.12c |mug108||sequence orphan|Schizosaccharomyces pombe|... 28 8.4
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 28 8.4
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 28 8.4
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 28 8.4
>SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1328
Score = 292 bits (716), Expect = 2e-79
Identities = 135/277 (48%), Positives = 178/277 (64%), Gaps = 2/277 (0%)
Query: 1 MAIDGVAPRAKMNQQRGRRFRSXXXXXXXXXXXXXXXXVLPTEKRFDSNCITPGTVFMAR 60
MA+DG APRAKMNQQR RRFR+ P E++FDSNCITPGT FM R
Sbjct: 83 MAVDGCAPRAKMNQQRSRRFRTAKDAHDARLKAERNGEDFP-EEQFDSNCITPGTTFMER 141
Query: 61 LHEQLKYFVKHKMSTDPLWSKVKVILSGHETPGEGEHKIMDYIRWARSQPDYDPNTRHCL 120
+ QL YF+ K++ D W ++VI SGH+ PGEGEHKIM+YIR ++QP Y+PNTRHCL
Sbjct: 142 VSRQLYYFIHKKVTNDSQWQNIEVIFSGHDCPGEGEHKIMEYIRTQKAQPSYNPNTRHCL 201
Query: 121 YGLDADLIMLGMCTHEPHFALLREEVKFGRTTQ-RATTPEETNFXXXXXXXXXXXXXQEF 179
YGLDADLIMLG+ +H+PHF LLREEV FG ++ R+ F EF
Sbjct: 202 YGLDADLIMLGLLSHDPHFCLLREEVTFGPASRNRSKELAHQKFYLLHLSLLREYLEFEF 261
Query: 180 IAIKDKLPFPYDIEKIVDDWVLMGFLVGNDFIPNLPNMHVNNDALPLLYNTYMTVLPKLD 239
+ F YD+EKI+DD++L+ F VGNDF+P+LP +H+N AL L+++ Y V+P
Sbjct: 262 QECRSTFTFKYDLEKILDDFILLAFFVGNDFLPHLPGLHINEGALALMFSIYKKVMPSAG 321
Query: 240 GYINEAGDLNLERFEIFMQELSKIDKEQFQDTYTDLK 276
GYINE G +N+ R E+ + EL +KE F+ ++ K
Sbjct: 322 GYINEKGVINMARLELILLELENFEKEIFKAEVSETK 358
Score = 256 bits (628), Expect = 1e-68
Identities = 158/445 (35%), Positives = 236/445 (53%), Gaps = 22/445 (4%)
Query: 349 NMEMEFILHKKDYYMNKLDYSKVTDEVLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHH 408
N+E +FI K DYY +K+ +S +E L AE YV +QW L+YYY+GC SW W+Y +H
Sbjct: 488 NVEKDFIQWKDDYYRSKVGFSYYDEEALKAMAERYVEGLQWVLFYYYRGCQSWGWYYNYH 547
Query: 409 YAPYISDIKGFKDLKIDFELGEPFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHY 468
+AP ISD+ D+KIDF++G PF+PFEQL+AVLPA S+ L+P F DLM + +SPI+ +
Sbjct: 548 FAPKISDVLKGLDVKIDFKMGTPFRPFEQLMAVLPARSQALVPPCFRDLMVNSESPIIDF 607
Query: 469 YPVHFDTDLNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLTDEEIKRNQPGPMLVYSWS 528
YP +F D NGK WEAVV+IPFIDET L+ A+A + LT+EE KRN V+S +
Sbjct: 608 YPENFALDQNGKTASWEAVVIIPFIDETRLIDALASKDKFLTEEERKRNSFNAPTVFSLA 667
Query: 529 S--TSLGVVDAPQYFPSIKECHAFEKRVWRNELDVPEEQLKKGMLPNARRDML-FPGFPT 585
TS P FP + +K ++ E + G+ P GFP+
Sbjct: 668 EDYTSFYPSSLPSLFPDL-VTRCIQKPYSLPSMEGKEYLV--GLCPGVFLGAFGMVGFPS 724
Query: 586 MRHLKYKSSHKMAKVKVFDLPARNSSVIIELIRDKDEDSLERVANELLGKVIWVGWPHLT 645
LK+K+ + VF +RN SVI+ + K + E++A + +GK I+V WP+L
Sbjct: 725 FHTLKHKAELVYHGINVFGNESRNPSVIVNVEDVKSALTSEQIAMQYVGKRIFVDWPYLR 784
Query: 646 RAKVISVSNEKTRLHYVDKQNNNVNGTSLYTTVQNSGNLHKQWLGERNTIIEHNMNRLGI 705
A V S +E Y+ +N+ ++ S QW + + I R G+
Sbjct: 785 EAYVESAMDES----YMYLASNSTIEKRDLAEIEKS-----QWGRKCSHKIREYSKRFGV 835
Query: 706 ELGDVNIIVHAVNLKGYKYIVQDNCQMVYEPEWCEVPCGYAYQAVVRDVSAHVVQEVNRF 765
GD+++++ +KG +Y + ++ E Y Q VV ++ + R
Sbjct: 836 LFGDISLLLQVRPIKGLEYTREGALVKIFNESVLE---DYPAQLVVEKIAIDDPRFTERE 892
Query: 766 S-TLQEAYPPGDTVFLLTPGQY-YG 788
+ ++ YPPG F L G+Y YG
Sbjct: 893 APPVEVEYPPGTKAFHL--GEYNYG 915
Score = 36.3 bits (80), Expect = 0.024
Identities = 15/36 (41%), Positives = 25/36 (69%), Gaps = 2/36 (5%)
Query: 928 QEVAGYTRRCAVTGAWQYSDRCVQLVKDYAQNFPEL 963
Q+V G++R+ T W++S++ V LVK+Y FP+L
Sbjct: 1002 QKVLGFSRKS--TKGWEFSNKTVALVKEYINTFPQL 1035
>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 991
Score = 235 bits (574), Expect = 3e-62
Identities = 132/332 (39%), Positives = 186/332 (56%), Gaps = 33/332 (9%)
Query: 1 MAIDGVAPRAKMNQQRGRRFRSXXXXXXXXXXXXXXXXV-----LPTE------KRFDSN 49
+AIDGVAPRAKMNQQR RRFRS +P + K +DSN
Sbjct: 101 IAIDGVAPRAKMNQQRSRRFRSSREAALKEEELQAFIEEAKQQGIPIDENATKKKSWDSN 160
Query: 50 CITPGTVFMARLHEQLKYFVKHKMSTDPLWSKVKVILSGHETPGEGEHKIMDYIRWARSQ 109
CITPGT FM L + L+Y++ +K+++DP W V+ ILS PGEGEHKIM++IR R +
Sbjct: 161 CITPGTPFMDTLAKSLRYYIINKLNSDPCWRNVRFILSDASVPGEGEHKIMEFIRSQRVK 220
Query: 110 PDYDPNTRHCLYGLDADLIMLGMCTHEPHFALLREEVKFGRTTQRATTPE---------- 159
P+YDPNT H +YGLDADLIMLG+ THEPHF +LRE+V F + + + T E
Sbjct: 221 PEYDPNTHHVVYGLDADLIMLGLATHEPHFRVLREDVFFQQGSTKKTKEERLGIKRLDDV 280
Query: 160 -ETNF--XXXXXXXXXXXXXQEFIAIK---DKLPFPYDIEKIVDDWVLMGFLVGNDFIPN 213
ETN +E++ ++ LPFP+D+E+ +DDWV F VGNDF+P+
Sbjct: 281 SETNKVPVKKPFIWLNVSILREYLEVELYVPNLPFPFDLERAIDDWVFFIFFVGNDFLPH 340
Query: 214 LPNMHVNNDALPLLYNTYMTVLPKLDGYINEAGDLNLERFEIFMQELSKIDKEQFQDTYT 273
LP++ + + A+ L + LP + GY+ G +NL R E+ + + Q D +
Sbjct: 341 LPSLDIRDGAVERLTEIWRASLPHMGGYLTLDGSVNLARAEVILSAVG----NQEDDIFK 396
Query: 274 DLKYFEEKTGRRPNANERREYKANNNETFNVD 305
LK E++ R N R++ ++N VD
Sbjct: 397 RLKQQEDR--RNENYRRRQQRESNQESESYVD 426
Score = 192 bits (468), Expect = 2e-49
Identities = 82/169 (48%), Positives = 114/169 (67%)
Query: 357 HKKDYYMNKLDYSKVTDEVLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHHYAPYISDI 416
+++ YY K S E + + + YV + W L YYY+GCPSW W+YP+HYAP+ +D
Sbjct: 591 YRERYYEQKFHISPDEPEKIREAVKHYVHGLCWVLLYYYQGCPSWTWYYPYHYAPFAADF 650
Query: 417 KGFKDLKIDFELGEPFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHYYPVHFDTD 476
K + + FEL +PFKP+EQLL VLPAASK+ LP LMTDE+S I+ +YP +F D
Sbjct: 651 KDLASIDVKFELNQPFKPYEQLLGVLPAASKNNLPEKLQTLMTDENSEIIDFYPENFTID 710
Query: 477 LNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLTDEEIKRNQPGPMLVY 525
LNGK +W+ V L+PFIDE LL+A++ Y +LT+EE KRN+ G L++
Sbjct: 711 LNGKKFEWQGVALLPFIDENRLLNAVSKIYPQLTEEESKRNEDGSTLLF 759
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 33.1 bits (72), Expect = 0.22
Identities = 25/96 (26%), Positives = 46/96 (47%), Gaps = 6/96 (6%)
Query: 229 NTYMTVLPKLDGYINEAGDLNLERFEIFMQELSKIDKEQFQDTYTDLKYFEEKTGRRPNA 288
N +LPK + ++EA DLN + Q+ S +DK+ +T K +E
Sbjct: 342 NELSEILPKYNAIVSEADDLNKRIMLLKNQKQSLLDKQSRTSQFTTKKERDEWI-----R 396
Query: 289 NERREYKANNNET-FNVDVNEIKGNHLDNDLQALIN 323
N+ + N N T N D + + + ++N+L+A ++
Sbjct: 397 NQLLQINRNINSTKENSDYLKTEYDEMENELKAKLS 432
>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 29.9 bits (64), Expect = 2.1
Identities = 19/82 (23%), Positives = 43/82 (52%), Gaps = 3/82 (3%)
Query: 248 LNLERFEIFMQELSKIDKEQFQDTYTDLKYFEEKTGRRPNANERREYKANNNETF-NVDV 306
++ R ++F + L ++D+E+ Y+D++ TG P E +E F + +
Sbjct: 731 IHFAREKVFPR-LQQMDEEKISRLYSDMRRESLATGSYPITVRHLESAIRLSEAFAKMQL 789
Query: 307 NE-IKGNHLDNDLQALINATDN 327
+E ++ +H+D +Q +I++ N
Sbjct: 790 SEFVRPSHIDKAIQVIIDSFVN 811
>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 500
Score = 29.5 bits (63), Expect = 2.8
Identities = 22/89 (24%), Positives = 40/89 (44%), Gaps = 5/89 (5%)
Query: 232 MTVLPKLDGY-INEAGDLNLERFEIFMQELSKIDKEQFQDTYTDLKYFEEKTGRRPNANE 290
+T+ +LD + + E+GDL++ F E + D F +Y +K N +
Sbjct: 262 ITIQKRLDEFKLTESGDLSIADFRNIWLE-GQSDPPSFTKNQKFQQYGAQKVS---NIDH 317
Query: 291 RREYKANNNETFNVDVNEIKGNHLDNDLQ 319
+EY + T + D NE+K L ++
Sbjct: 318 TQEYMSPIKRTPDFDGNEVKSEELSQTVK 346
>SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein
Urb1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1568
Score = 28.7 bits (61), Expect = 4.8
Identities = 18/66 (27%), Positives = 35/66 (53%), Gaps = 6/66 (9%)
Query: 208 NDFIPN--LPNMHVNNDALPLLYNTYMTVLPKLDGYINEAGDLNLERFEIFMQELSKIDK 265
ND + N + +N D LPL Y+ ++ K + ++GD+ +++F ++EL + K
Sbjct: 709 NDELSNSGIQKFGMNLDFLPL----YVVIITKRIAIMLQSGDMRVKKFLELIRELVEFSK 764
Query: 266 EQFQDT 271
E+ T
Sbjct: 765 EETMAT 770
>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 997
Score = 27.9 bits (59), Expect = 8.4
Identities = 26/107 (24%), Positives = 46/107 (42%), Gaps = 6/107 (5%)
Query: 639 VGWPHLTRA--KVISVSNEKTRLHYVDKQNNNVNGTSLYTTVQNSGNLHKQWLGERNTII 696
V W H+T A + +S ++ Y+ + N LY LHK LG + ++
Sbjct: 759 VEWSHITLAGASTLGLSQTRSSEFYMKSRENGTIPRPLYVISHLRPLLHKTSLGVGDILL 818
Query: 697 EHNMNRLGIELGDVNIIVHAVNLKGYKYIVQDNCQM-VYEPEWCEVP 742
E N ++ L D++ +K I++D +M + P + E P
Sbjct: 819 EVN-GKMITRLSDLHEFETESEIKA--VILRDGIEMEITIPLYPEYP 862
>SPAC29A4.12c |mug108||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 27.9 bits (59), Expect = 8.4
Identities = 16/44 (36%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Query: 278 FEEKTGRRPNANERREYKANNNETFNVDVNEIKGNHLDNDLQAL 321
F K R +AN ++ ANNNE +D E +LD D+ L
Sbjct: 104 FGGKKSRSKSANGTQKKNANNNEE-QLDEEEQNDPYLDRDISLL 146
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 27.9 bits (59), Expect = 8.4
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Query: 931 AGYTRRCAVTGAWQYSDRCVQLVKDYAQNFPELEKKYQDVLQWIKNQP 978
AG+TR A+ + D L DY NF K+Q++LQ I++ P
Sbjct: 1029 AGHTRNTALYIYGRTMDNLHYLPSDYFANFFRASYKWQELLQ-IRDNP 1075
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 27.9 bits (59), Expect = 8.4
Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Query: 931 AGYTRRCAVTGAWQYSDRCVQLVKDYAQNFPELEKKYQDVLQWIKNQP 978
AG+TR A+ + D L DY NF K+Q++LQ I++ P
Sbjct: 1029 AGHTRNTALYIYGRTMDNLHYLPSDYFANFFRASYKWQELLQ-IRDNP 1075
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 27.9 bits (59), Expect = 8.4
Identities = 20/74 (27%), Positives = 29/74 (39%), Gaps = 3/74 (4%)
Query: 447 KHLLPTPFHDLMTDEDSPIVHYYPVHFDTDLNGKINDWEAVVLIPFIDET-NLLSAMAPC 505
K TPF D TD H P+H ++ ++D + P + T N+ MAP
Sbjct: 195 KEASETPFRDASTDLFDEHAHAAPMHSSISISTLLSDSDRYE--PHVSLTENISPVMAPS 252
Query: 506 YQRLTDEEIKRNQP 519
+ I R P
Sbjct: 253 IDARLSQTILRGLP 266
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.320 0.136 0.420
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,066,936
Number of Sequences: 5004
Number of extensions: 214845
Number of successful extensions: 529
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 512
Number of HSP's gapped (non-prelim): 24
length of query: 1120
length of database: 2,362,478
effective HSP length: 80
effective length of query: 1040
effective length of database: 1,962,158
effective search space: 2040644320
effective search space used: 2040644320
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 59 (27.9 bits)
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