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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000473-TA|BGIBMGA000473-PA|IPR004859|Putative 5-3
exonuclease
         (1120 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_15962| Best HMM Match : DUF528 (HMM E-Value=0.083)                 289   1e-77
SB_5082| Best HMM Match : Fn_bind (HMM E-Value=6.8)                   113   1e-24
SB_8914| Best HMM Match : No HMM Matches (HMM E-Value=.)               35   0.32 
SB_5079| Best HMM Match : fn3 (HMM E-Value=1.3e-35)                    35   0.32 
SB_25519| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   1.3  
SB_35738| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   1.7  
SB_4521| Best HMM Match : TPR_2 (HMM E-Value=1.7e-13)                  32   2.3  
SB_41904| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   3.0  
SB_2561| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   5.2  
SB_30958| Best HMM Match : rve (HMM E-Value=2.4e-15)                   31   6.9  
SB_24724| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   6.9  
SB_3031| Best HMM Match : NblA (HMM E-Value=8.4)                       30   9.1  
SB_57078| Best HMM Match : Xlink (HMM E-Value=5.8)                     30   9.1  
SB_7443| Best HMM Match : Xlink (HMM E-Value=5.8)                      30   9.1  

>SB_15962| Best HMM Match : DUF528 (HMM E-Value=0.083)
          Length = 1466

 Score =  289 bits (708), Expect = 1e-77
 Identities = 137/290 (47%), Positives = 192/290 (66%), Gaps = 3/290 (1%)

Query: 371 VTDEVLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHHYAPYISDIKGFKDLKIDFELGE 430
           ++ +V++     YVR IQW L+YY+ G  SW W+YP HYAP++SDIK F   ++ F++ +
Sbjct: 274 LSSDVVSKITLEYVRGIQWILHYYFNGVQSWSWYYPFHYAPFLSDIKDFSHYELKFDMSK 333

Query: 431 PFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHYYPVHFDTDLNGKINDWEAVVLI 490
           PF PFEQLL VLPAASK LLP PF +LM  E SPI+ YYP++F TDLNGK  +WEAVVLI
Sbjct: 334 PFLPFEQLLGVLPAASKQLLPAPFQNLMIMEQSPIIDYYPLNFKTDLNGKQQEWEAVVLI 393

Query: 491 PFIDETNLLSAMAPCYQRLTDEEIKRNQPGPMLVYSWSSTSLGVVDA--PQYFPSIKECH 548
           PFIDE  LL AM P Y++LT EE  RN  GP  +Y++  T      +  P  FP I  CH
Sbjct: 394 PFIDEKRLLEAMKPLYRQLTKEEKTRNTHGPCHLYTYDKTLADHYPSTLPGVFPDISRCH 453

Query: 549 AFEKRVWRNELDVPEEQLKKGMLPNARRDMLFPGFPTMRHLKYKSSHKMAKVKVFDLPAR 608
           A  ++  R +L    E L KG+L + + ++ FPGFPT+ H+ + +  K A VKVF + +R
Sbjct: 454 ARCEKFDREQLVSSLENLVKGLLKDVKLEVYFPGFPTLYHIPHTAELKKASVKVFQMHSR 513

Query: 609 NSSVIIELIRDKDEDSLERVANELLGKVIWVGWPHLTRAKVISVSNEKTR 658
           + S+I++L + + E ++ RVA + LG+  +VGWPH+  A V++VS+   R
Sbjct: 514 SDSMILKLQQSQLEPTI-RVAEKYLGETCYVGWPHMVEALVVAVSDNTFR 562



 Score =  149 bits (361), Expect = 1e-35
 Identities = 79/209 (37%), Positives = 115/209 (55%), Gaps = 15/209 (7%)

Query: 184 DKLPFPYDIEKIVDDWVLMGFLVGNDFIPNLPNMHVNNDALPLLYNTYMTVLPKLDGYIN 243
           D LPF YD+E+I+DDW+LMGFLVGNDFIP+LP MH+N+DALP+LY  Y  ++P LDGY++
Sbjct: 29  DSLPFEYDLERIIDDWILMGFLVGNDFIPHLPYMHINHDALPMLYQIYKEIMPSLDGYLH 88

Query: 244 EAGDLNLERFEIFMQELSKIDKEQFQDTYTDLKYFE--------EKTGRRP---NANERR 292
           + G L L RFE +++ELSK D+E+F +++ D+K+FE        EK G R    N   R+
Sbjct: 89  DGGTLCLPRFEKYLKELSKFDREKFNESFVDMKWFEAKVKHKFGEKKGARHSQLNPKVRQ 148

Query: 293 EYKANNNETFNVDVNEIKGNHLDNDLQALINATDNMFMNXXXXXXXXXXXXXXXXANMEM 352
              +++N    +D   ++      D    I+      +                    E+
Sbjct: 149 GNDSSDNPFSALDSLAVEFEEFKTDDDPEISPE----LAKLAAKMDDGDGDSEEVDEFEI 204

Query: 353 EFILHKKDYYMNKLDYSKVTDEVLADQAE 381
           EF +HKK YY +K     V  +  A   E
Sbjct: 205 EFAMHKKSYYSDKFGLEHVDRQFSASSWE 233



 Score = 56.0 bits (129), Expect = 2e-07
 Identities = 28/86 (32%), Positives = 45/86 (52%), Gaps = 2/86 (2%)

Query: 723 KYIVQDNCQMVYEPEWCEVPCGYAYQAVVRDVSAHVVQEVNRFSTLQEAYPPGDTVFLLT 782
           +++     Q+  E EW  VP  YA+Q VV+D+S H     +   T+ E +P     F+L 
Sbjct: 593 RFLCSAKGQVTLEKEWSPVPKQYAFQTVVKDISVHESVSTDSMRTIDELFPVDCVCFMLA 652

Query: 783 PGQYYGCLATVVESAQVRSGRIKVSV 808
              +YG   TV+E   V+ GR++V +
Sbjct: 653 M-PHYGASGTVLE-VDVKQGRVRVQL 676



 Score = 53.2 bits (122), Expect = 1e-06
 Identities = 21/28 (75%), Positives = 25/28 (89%)

Query: 100 MDYIRWARSQPDYDPNTRHCLYGLDADL 127
           M++IR  R++PDYD NTRHCLYGLDADL
Sbjct: 1   MEFIRTERTRPDYDANTRHCLYGLDADL 28



 Score = 50.0 bits (114), Expect = 1e-05
 Identities = 44/166 (26%), Positives = 72/166 (43%), Gaps = 34/166 (20%)

Query: 928  QEVAGYTRRCAVTGAWQYSDRCVQLVKDYAQNFPEL----------EKKYQ--------- 968
            +E+ GYTRR      W ++     ++  Y   FP+L          ++ Y+         
Sbjct: 691  RELLGYTRR--TQNGWMFTQAARNVITQYINKFPKLFDALATLQQSDQVYEADMLDHGID 748

Query: 969  -----DVLQWIKNQPFRRALRRDCGSEALEPEEMKALADNIDK-QLEILNANERKITMQV 1022
                 DV  W+K+ P ++     CGS+AL+   ++A+ + + K ++  L A E  +    
Sbjct: 749  LSVLTDVQNWLKSLPLQKQTVVTCGSDALDDPVLEAIEEAVKKVKVSYLEAIEGAVRKVK 808

Query: 1023 KWNLLYKAELHAGEVSPDRAADYRLYDRVVCVARGLCVPAGARGTV 1068
              + L       G   PD   + +L+DRVV V     VP G RGTV
Sbjct: 809  PSDFL-------GNTLPDSDTEIQLFDRVVNVKLNSAVPFGLRGTV 847


>SB_5082| Best HMM Match : Fn_bind (HMM E-Value=6.8)
          Length = 367

 Score =  113 bits (271), Expect = 1e-24
 Identities = 65/166 (39%), Positives = 90/166 (54%), Gaps = 32/166 (19%)

Query: 358 KKDYYMNKLDYSKVT---DEVLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHHYAPYIS 414
           K  YY NK   S+     D+   + A+ YV  + W L YYY+GCPSW W+YP+HYAP+ S
Sbjct: 135 KGRYYSNKFGVSESDEGFDDFRKNVADAYVEGLCWVLRYYYQGCPSWKWYYPYHYAPFAS 194

Query: 415 DIKGFKDLKIDFELGEPFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHYYPVHFD 474
           D   F+DL      G+    FE+                     T  +SPI+ +YPV F+
Sbjct: 195 D---FRDL------GQLKDEFER--------------------GTKPESPIIDFYPVDFE 225

Query: 475 TDLNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLTDEEIKRNQPG 520
            DLNGK   W+ V L+PF+DE  LL+A+A  Y  ++++E KRN  G
Sbjct: 226 IDLNGKKFAWQGVALLPFVDEKRLLNALAKVYPDISEDERKRNSLG 271


>SB_8914| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1142

 Score = 35.1 bits (77), Expect = 0.32
 Identities = 30/96 (31%), Positives = 45/96 (46%), Gaps = 3/96 (3%)

Query: 416 IKGFKDLKIDFELGEPFKPFEQLLAVLPAASK-HLLPTPFHDLMTDEDSPIVHYYPVHFD 474
           +KG +DL+ D  + + F     LLA  P  SK HL    +  +    +S ++ + P H D
Sbjct: 700 LKGHEDLRQDERVMQLFGLVNTLLASDPETSKRHLSIQRYAVIPLSTNSGLIGWVP-HCD 758

Query: 475 TDLNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLT 510
           T L+  I D+     I    E  ++  MAP Y  LT
Sbjct: 759 T-LHALIRDYREKKKILLNIEHRIMLTMAPDYDHLT 793


>SB_5079| Best HMM Match : fn3 (HMM E-Value=1.3e-35)
          Length = 978

 Score = 35.1 bits (77), Expect = 0.32
 Identities = 14/35 (40%), Positives = 22/35 (62%)

Query: 54  GTVFMARLHEQLKYFVKHKMSTDPLWSKVKVILSG 88
           GT FM  L   L+Y++  +++TDP W  +K I +G
Sbjct: 797 GTEFMDNLSISLRYYIADRLNTDPGWRNIKNIPAG 831


>SB_25519| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 45

 Score = 33.1 bits (72), Expect = 1.3
 Identities = 16/34 (47%), Positives = 19/34 (55%)

Query: 1035 GEVSPDRAADYRLYDRVVCVARGLCVPAGARGTV 1068
            G   PD   + +L+DRVV V     VP G RGTV
Sbjct: 6    GNTLPDSDTEIQLFDRVVNVKLNSAVPFGLRGTV 39


>SB_35738| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1186

 Score = 32.7 bits (71), Expect = 1.7
 Identities = 23/97 (23%), Positives = 48/97 (49%), Gaps = 9/97 (9%)

Query: 929  EVAGYTRRCAVTGAWQYSDRCVQLVKDYAQNFPELEKKYQDVLQWIKNQPFRRALRRD-C 987
            +   Y +  AV  A +  +R  + ++ Y Q++   +++ Q +L W +  P +  L R   
Sbjct: 740  DAMNYAKMTAVVAAEK--ERHDKALEKYQQDYGRYQQRRQKILDWRERVPRKAELHRHRL 797

Query: 988  GSEALEPEEMKAL--ADNIDKQLEIL----NANERKI 1018
            G++ L+P+  K +   D   + + +L    NA ER++
Sbjct: 798  GAQTLQPDPAKTIPGVDAGPRAVRLLHPQQNAEERRV 834


>SB_4521| Best HMM Match : TPR_2 (HMM E-Value=1.7e-13)
          Length = 829

 Score = 32.3 bits (70), Expect = 2.3
 Identities = 27/98 (27%), Positives = 45/98 (45%), Gaps = 4/98 (4%)

Query: 664 KQNNNVNGTSLYTTVQNSGNLHKQWLGERNTIIEHNMNRLGIELGDVNIIVHAVNLKGYK 723
           KQ   + G SL   ++ S  + ++ LGE +T     +N +G  L  +N    A  LK Y+
Sbjct: 602 KQKEGIAGESLLE-LEYSLEIRRKQLGE-HTQTSRTLNLMGNALMALNKPEEA--LKRYQ 657

Query: 724 YIVQDNCQMVYEPEWCEVPCGYAYQAVVRDVSAHVVQE 761
             +    +++   E CE+P  Y   A V +  +   QE
Sbjct: 658 EAMNMKTRLIGSEEHCEMPTFYNQMAAVHERLSEKTQE 695


>SB_41904| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 89

 Score = 31.9 bits (69), Expect = 3.0
 Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 4/54 (7%)

Query: 969  DVLQWIKNQPFRRALRRDCGSEALEPEEMKALADNIDK----QLEILNANERKI 1018
            DV  W+K+ P ++     CGS+AL+   ++A+ + + K     LE +    RK+
Sbjct: 35   DVQNWLKSLPLQKQTVVTCGSDALDDPVLEAIEEAVKKVKVSYLEAIEGAVRKV 88


>SB_2561| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 649

 Score = 31.1 bits (67), Expect = 5.2
 Identities = 23/84 (27%), Positives = 42/84 (50%), Gaps = 6/84 (7%)

Query: 545 KECHAFEKRVWRNELDVPEEQLKKGMLPNARRDMLFPGFPTMRH-----LKYKSSHKMAK 599
           KE    +K+  R +   PE  +++ +    +R ++F  F   R      LK  S  +   
Sbjct: 530 KEAAKRKKQEQRQDDTKPENTVQEEVNKPKKRQIMFSVFGDYRKKMADDLKTYSGKERKS 589

Query: 600 VKVFDLP-ARNSSVIIELIRDKDE 622
           VK + +P A   SV+I++I++KD+
Sbjct: 590 VKAYAVPKAEQKSVVIKVIKNKDQ 613


>SB_30958| Best HMM Match : rve (HMM E-Value=2.4e-15)
          Length = 470

 Score = 30.7 bits (66), Expect = 6.9
 Identities = 19/79 (24%), Positives = 41/79 (51%), Gaps = 7/79 (8%)

Query: 947  DRCVQLVKDYAQNFPELEKKYQDVLQWIKNQPFRRALRRD-CGSEALEPEEMKAL--ADN 1003
            +R  + ++ Y Q++   +++ Q +L W +  P +  L R   G++ L+P+  K +   D 
Sbjct: 40   ERHDKALEKYQQDYGRYQQRRQKILDWRERVPRKAELHRHRLGAQTLQPDPAKTIPGVDA 99

Query: 1004 IDKQLEIL----NANERKI 1018
              + + +L    NA ER++
Sbjct: 100  GPRAVRLLHPQQNAEERRV 118


>SB_24724| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2021

 Score = 30.7 bits (66), Expect = 6.9
 Identities = 16/49 (32%), Positives = 25/49 (51%)

Query: 510  TDEEIKRNQPGPMLVYSWSSTSLGVVDAPQYFPSIKECHAFEKRVWRNE 558
            TDE++    PG +  Y+ SSTSL +   P  FP +       + ++R E
Sbjct: 1177 TDEDVPSLAPGNLQAYNTSSTSLNITWNPIPFPYVHGILRGYRAMYREE 1225


>SB_3031| Best HMM Match : NblA (HMM E-Value=8.4)
          Length = 256

 Score = 30.3 bits (65), Expect = 9.1
 Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 4/80 (5%)

Query: 563 EEQLKKGMLPNARRDMLF-PGFPTMRHLKYKSSHKMAKVKVFDLPARNSS--VIIELIRD 619
           E  L + +     RD++  P   T R L    S +  +  V D P+R +   ++ +L+RD
Sbjct: 75  ERDLMRDLSRKTERDLVRDPTRETGRDLVRDPSRETERDLVRD-PSRETGRDLVRDLLRD 133

Query: 620 KDEDSLERVANELLGKVIWV 639
            D D L  +  +LL  ++WV
Sbjct: 134 LDSDLLRDLTRDLLRDLLWV 153


>SB_57078| Best HMM Match : Xlink (HMM E-Value=5.8)
          Length = 233

 Score = 30.3 bits (65), Expect = 9.1
 Identities = 17/68 (25%), Positives = 29/68 (42%), Gaps = 2/68 (2%)

Query: 937  CAVTGAWQYSDRCVQLVKDYAQNFPELEKKYQDVLQWIKNQPFRRALRRDCGSEALEPEE 996
            C V   + Y  R   ++  + ++ PEL     +V +WI N    R    +  +  L P  
Sbjct: 98   CVVLKRFAYPCRLSDMISIFGRSVPELSMICNEVTEWIYNAHHHRT--TEWNNFILSPNS 155

Query: 997  MKALADNI 1004
            ++  AD I
Sbjct: 156  LQTYADAI 163


>SB_7443| Best HMM Match : Xlink (HMM E-Value=5.8)
          Length = 233

 Score = 30.3 bits (65), Expect = 9.1
 Identities = 17/68 (25%), Positives = 29/68 (42%), Gaps = 2/68 (2%)

Query: 937  CAVTGAWQYSDRCVQLVKDYAQNFPELEKKYQDVLQWIKNQPFRRALRRDCGSEALEPEE 996
            C V   + Y  R   ++  + ++ PEL     +V +WI N    R    +  +  L P  
Sbjct: 98   CVVLKRFAYPCRLSDMISIFGRSVPELSMICNEVTEWIYNAHHHRT--TEWNNFILSPNS 155

Query: 997  MKALADNI 1004
            ++  AD I
Sbjct: 156  LQTYADAI 163


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.320    0.136    0.420 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 36,652,037
Number of Sequences: 59808
Number of extensions: 1505185
Number of successful extensions: 3392
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 3372
Number of HSP's gapped (non-prelim): 25
length of query: 1120
length of database: 16,821,457
effective HSP length: 90
effective length of query: 1030
effective length of database: 11,438,737
effective search space: 11781899110
effective search space used: 11781899110
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 65 (30.3 bits)

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