Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000473-TA|BGIBMGA000473-PA|IPR004859|Putative 5-3
exonuclease
(1120 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g75660.1 68414.m08789 5'-3' exoribonuclease (XRN3) identical ... 211 2e-54
At1g54490.1 68414.m06215 5'-3' exoribonuclease (XRN4) identical ... 205 1e-52
At5g42540.1 68418.m05178 5'-3' exoribonuclease (XRN2) identical ... 173 7e-43
At4g24900.1 68417.m03564 expressed protein 31 3.3
At3g11380.1 68416.m01384 pentatricopeptide (PPR) repeat-containi... 31 3.3
At5g46280.1 68418.m05697 DNA replication licensing factor, putat... 31 4.3
At5g44230.1 68418.m05411 pentatricopeptide (PPR) repeat-containi... 31 4.3
At2g03480.2 68415.m00308 dehydration-responsive protein-related ... 31 4.3
At2g03480.1 68415.m00307 dehydration-responsive protein-related ... 31 4.3
At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein heli... 31 5.7
At1g63020.1 68414.m07117 DNA-directed RNA polymerase alpha subun... 31 5.7
At3g46670.1 68416.m05066 UDP-glucoronosyl/UDP-glucosyl transfera... 30 10.0
>At1g75660.1 68414.m08789 5'-3' exoribonuclease (XRN3) identical to
XRN3 [Arabidopsis thaliana] gi|11875628|gb|AAG40732
Length = 1020
Score = 211 bits (515), Expect = 2e-54
Identities = 124/307 (40%), Positives = 164/307 (53%), Gaps = 38/307 (12%)
Query: 1 MAIDGVAPRAKMNQQRGRRFRSXXXXXXXXXXXXXXXXVLPTEKR----------FDSNC 50
MAIDGVAPRAKMNQQR RRFRS E R FDSN
Sbjct: 100 MAIDGVAPRAKMNQQRSRRFRSAKDASDAAAEEERLREEFEREGRRLPPKVDSQVFDSNV 159
Query: 51 ITPGTVFMARLHEQLKYFVKHKMSTDPLWSKVKVILSGHETPGEGEHKIMDYIRWARSQP 110
ITPGT FM L L+Y+V +++ D W +KVILS PGEGEHKIM YIR R+ P
Sbjct: 160 ITPGTEFMGVLSIALQYYVHLRLNHDVGWKNIKVILSDANVPGEGEHKIMSYIRLQRNLP 219
Query: 111 DYDPNTRHCLYGLDADLIMLGMCTHEPHFALLREEVKF---------------------G 149
+DPNTRHCLYGLDADLIMLG+ THE HF++LRE V G
Sbjct: 220 GFDPNTRHCLYGLDADLIMLGLATHEVHFSILREVVYTPGQQERCFLCGQMGHFASNCEG 279
Query: 150 RTTQRATTPEE----TNFXXXXXXXXXXXXXQEFIAIKDKL---PFPYDIEKIVDDWVLM 202
+ +RA +E +F +E++ ++ ++ PF D+E+IVDD++ +
Sbjct: 280 KPKKRAGESDEKGDGNDFVKKPYQFLHIWVLREYLELEMRIPNPPFEIDLERIVDDFIFI 339
Query: 203 GFLVGNDFIPNLPNMHVNNDALPLLYNTYMTVLPKLDGYINEAGDLNLERFEIFMQELSK 262
F VGNDF+P++P + + A+ LL Y DGY+ + NL+R E F+Q +
Sbjct: 340 CFFVGNDFLPHMPTLEIREGAINLLMAVYKKEFRSFDGYLTDGCKPNLKRVEQFIQAVGS 399
Query: 263 IDKEQFQ 269
+ + FQ
Sbjct: 400 FEDKIFQ 406
Score = 165 bits (401), Expect = 1e-40
Identities = 78/164 (47%), Positives = 102/164 (62%), Gaps = 6/164 (3%)
Query: 358 KKDYYMNKLDYSKVTDE----VLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHHYAPYI 413
++ YY K +S VT E V D Y + W ++YY +G SW WFYP+HYAP+
Sbjct: 539 RERYYEEK--FSVVTPEEMERVRKDVVLKYTEGLCWVMHYYMEGVCSWQWFYPYHYAPFA 596
Query: 414 SDIKGFKDLKIDFELGEPFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHYYPVHF 473
SD+K ++ I FELG PFKPF QLL V PAAS H LP + LMTD +SPI+ +YP F
Sbjct: 597 SDLKDLGEMDIKFELGTPFKPFNQLLGVFPAASSHALPERYRTLMTDPNSPIIDFYPTDF 656
Query: 474 DTDLNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLTDEEIKRN 517
+ D+NGK W+ + +PFIDE LL A++ LTDEE +RN
Sbjct: 657 EVDMNGKRFSWQGIAKLPFIDERRLLEAVSEVEFTLTDEEKRRN 700
>At1g54490.1 68414.m06215 5'-3' exoribonuclease (XRN4) identical to
XRN4 [Arabidopsis thaliana] GI:11875626; contains Pfam
domain PF03159: Putative 5'-3' exonuclease domain
Length = 947
Score = 205 bits (501), Expect = 1e-52
Identities = 119/303 (39%), Positives = 167/303 (55%), Gaps = 35/303 (11%)
Query: 1 MAIDGVAPRAKMNQQRGRRFRSXXXXXXXXXXXXXXXXVLPTEKRF----------DSNC 50
+AIDGVAPRAKMNQQR RRFR+ E + DSN
Sbjct: 101 LAIDGVAPRAKMNQQRSRRFRAAKDAAEAEAEEERLRKDFEMEGQILSAKEKAETCDSNV 160
Query: 51 ITPGTVFMARLHEQLKYFVKHKMSTDPLWSKVKVILSGHETPGEGEHKIMDYIRWARSQP 110
ITPGT FMA L L+Y+++ +++ +P W VKVILS PGEGEHKIM YIR R+ P
Sbjct: 161 ITPGTPFMAILSVALQYYIQSRLNHNPGWRYVKVILSDSNVPGEGEHKIMSYIRLQRNLP 220
Query: 111 DYDPNTRHCLYGLDADLIMLGMCTHEPHFALLREEVKF----------GRTTQRAT-TPE 159
+DPNTRHCLYGLDADLIML + THE HF++LRE + + G+T A+ P
Sbjct: 221 GFDPNTRHCLYGLDADLIMLSLATHEVHFSILREVITYPGQQEKCFVCGQTGHFASDCPG 280
Query: 160 ETNFXXXXXXXXXXXXXQEFI-------------AIKDKLPFPYDIEKIVDDWVLMGFLV 206
++ +F+ AI D PF + E+I+DD+V + F V
Sbjct: 281 KSGSNNAAADIPIHKKKYQFLNIWVLREYLQYELAIPDP-PFMINFERIIDDFVFLCFFV 339
Query: 207 GNDFIPNLPNMHVNNDALPLLYNTYMTVLPKLDGYINEAGDLNLERFEIFMQELSKIDKE 266
GNDF+P++P + + A+ LL + Y + GY+ ++G++ L+R E F+Q ++ + +
Sbjct: 340 GNDFLPHMPTLEIREGAINLLMHVYRKEFTAMGGYLTDSGEVLLDRVEHFIQAVAVNEDK 399
Query: 267 QFQ 269
FQ
Sbjct: 400 IFQ 402
Score = 165 bits (401), Expect = 1e-40
Identities = 78/179 (43%), Positives = 109/179 (60%), Gaps = 2/179 (1%)
Query: 357 HKKDYYMNKLDYS--KVTDEVLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHHYAPYIS 414
+K+ YY K + + T+++ D YV + W YYY+G SW WFYP+HYAP+ S
Sbjct: 444 YKERYYAEKFSTTNPEETEQIKQDMVLKYVEGLCWVCRYYYQGVCSWQWFYPYHYAPFAS 503
Query: 415 DIKGFKDLKIDFELGEPFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHYYPVHFD 474
D+K DL+I F +GEPFKPF+QL+ LPAAS + LP + LMTD SPI+ +YP F+
Sbjct: 504 DLKNLPDLEITFFIGEPFKPFDQLMGTLPAASSNALPGEYRKLMTDPSSPILKFYPADFE 563
Query: 475 TDLNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLTDEEIKRNQPGPMLVYSWSSTSLG 533
D+NGK W+ + +PFI+E LL+A + LT EE +RN L+Y + LG
Sbjct: 564 LDMNGKRFAWQGIAKLPFIEEKLLLAATRKLEETLTVEEQQRNSVMLDLLYVHPAHPLG 622
>At5g42540.1 68418.m05178 5'-3' exoribonuclease (XRN2) identical to
XRN2 [Arabidopsis thaliana] GI:11875630; contains Pfam
domain PF03159: Putative 5'-3' exonuclease domain
Length = 1012
Score = 173 bits (420), Expect = 7e-43
Identities = 80/178 (44%), Positives = 110/178 (61%), Gaps = 2/178 (1%)
Query: 358 KKDYYMNKLDYSKVTD--EVLADQAEGYVRAIQWNLYYYYKGCPSWCWFYPHHYAPYISD 415
++ YY K V + ++ D Y + W ++YYY G SW WFYP+HYAP+ SD
Sbjct: 531 RERYYEEKFAAKSVEEMEQIRRDVVLKYTEGLCWIMHYYYHGVCSWNWFYPYHYAPFASD 590
Query: 416 IKGFKDLKIDFELGEPFKPFEQLLAVLPAASKHLLPTPFHDLMTDEDSPIVHYYPVHFDT 475
+KG + L I FELG PFKPF QLLAVLP+AS H LP + LMT+ DSPI +YP F+
Sbjct: 591 LKGLEKLDIKFELGSPFKPFNQLLAVLPSASAHALPECYRSLMTNPDSPIADFYPADFEI 650
Query: 476 DLNGKINDWEAVVLIPFIDETNLLSAMAPCYQRLTDEEIKRNQPGPMLVYSWSSTSLG 533
D+NGK W+ + +PF++E LL A A + LT+EEI+RN +++ +S LG
Sbjct: 651 DMNGKRYSWQGISKLPFVEEKRLLEAAAQVEKSLTNEEIRRNSALFDMLFVVASHPLG 708
Score = 170 bits (414), Expect = 4e-42
Identities = 131/419 (31%), Positives = 186/419 (44%), Gaps = 43/419 (10%)
Query: 1 MAIDGVAPRAKMNQQRGRRFRSXXXXXXXXXXXXXXXXVLPTEKR----------FDSNC 50
MAIDGVAPRAKMNQQR RRFR+ E + FDSN
Sbjct: 102 MAIDGVAPRAKMNQQRARRFRAAKDAAEAAAEEEQLREEFEREGKKLPPKVDSQVFDSNV 161
Query: 51 ITPGTVFMARLHEQLKYFVKHKMSTDPLWSKVKVILSGHETPGEGEHKIMDYIRWARSQP 110
ITPGT FMA L L+Y++ ++++DP W +KVILS PGEGEHKIM YIR ++ P
Sbjct: 162 ITPGTEFMATLSFALRYYIHVRLNSDPGWKNIKVILSDANVPGEGEHKIMSYIRCNKNHP 221
Query: 111 DYDPNTRHCLYGLDADLIMLGMCTHEPHFALLREEVKFGRTTQRATTPEETNFXXXXXXX 170
Y+PNT HCLYGLDADLIML + THE HF++LRE V F + +
Sbjct: 222 GYNPNTHHCLYGLDADLIMLSLATHEIHFSILREVVFFPGEEGKCFLCGQEGHRAADCEG 281
Query: 171 XXXXXXQEFIAIKDKLPFPYDIEKIVDDWVLMGFLVGNDFIP----NLPNM--------- 217
E + + + V+ W+L +L + IP NL +
Sbjct: 282 KIKRKTGEMLDNTEADVVVKKPYEFVNIWILREYLEHDMQIPGAKKNLDRLIDDFIFICF 341
Query: 218 HVNNDALP-------------LLYNTYMTVLPKLDGYINEAGDLNLERFEIFMQELSKID 264
V ND LP LL + Y Y+ ++ LNL E F++ + +
Sbjct: 342 FVGNDFLPHMPTLEIREGAIELLMSVYKNKFRSAKKYLTDSSKLNLRNVERFIKAVGMYE 401
Query: 265 KEQFQDTYTDLKYFEEKTGRRPNANERREYKANNNETFNVDVNEIKGNHLDNDLQALINA 324
+ FQ ++ + + RR A + KA +N N + L L +L
Sbjct: 402 NQIFQKR-AQVQQRQSERFRRDKARD----KARDNARDNAQASRQFSGKL-VQLDSLDEV 455
Query: 325 TDNMFMNXXXXXXXXXXXXXXXXANMEMEFILHKKDYYMNKLDYSKVTDEVLADQAEGY 383
+D++ + AN+E E L K + N+ D ++L D+ +G+
Sbjct: 456 SDSLHSSPSRKYLRLSLDDNIGVANVETENSL-KAEELDNEEDLKFKLKKLLRDKGDGF 513
>At4g24900.1 68417.m03564 expressed protein
Length = 421
Score = 31.5 bits (68), Expect = 3.3
Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 6/49 (12%)
Query: 209 DFIPNLPNMHVN----NDALPLLYNT--YMTVLPKLDGYINEAGDLNLE 251
D I +P H+N ND +PL YNT Y L ++ G I+ LN++
Sbjct: 169 DRIKKVPAHHINSYKSNDVMPLQYNTNEYQISLSEIPGVIHNGSYLNMD 217
>At3g11380.1 68416.m01384 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 541
Score = 31.5 bits (68), Expect = 3.3
Identities = 20/71 (28%), Positives = 31/71 (43%), Gaps = 1/71 (1%)
Query: 238 LDGYINEAGDLNLERFEIFMQELSKIDKEQFQDTYTDLKYFEEKTGRRPNANE-RREYKA 296
L Y ++G+ L++ E +++ K+ Y + G R +E RE K
Sbjct: 150 LRSYARQSGEKALKKAESVFKKMKKLGLLLRPSPYNSMTSLYSSLGNRDKVDEILREMKE 209
Query: 297 NNNETFNVDVN 307
NN E NV VN
Sbjct: 210 NNVELDNVTVN 220
>At5g46280.1 68418.m05697 DNA replication licensing factor, putative
similar to SP|Q43704 DNA replication licensing factor
MCM3 homolog (Replication origin activator) (ROA
protein) {Zea mays}; contains Pfam profile PF00493:
MCM2/3/5 family
Length = 776
Score = 31.1 bits (67), Expect = 4.3
Identities = 21/59 (35%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Query: 248 LNLERFEIFMQELSKI-DKEQFQDTYTDLKYFEEKTGRRPNANERREYKANNNETFNVD 305
L L F I+ QEL+++ D+EQ + + +GRR N E A N+T NVD
Sbjct: 627 LKLMNFAIYHQELTEMDDREQEERQREQAEQERTPSGRRGNQRRNNEDGA-ENDTANVD 684
>At5g44230.1 68418.m05411 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 657
Score = 31.1 bits (67), Expect = 4.3
Identities = 13/25 (52%), Positives = 17/25 (68%)
Query: 930 VAGYTRRCAVTGAWQYSDRCVQLVK 954
VAGY CA GA +Y+DR VQ+ +
Sbjct: 284 VAGYISACAQLGASKYADRAVQIAQ 308
>At2g03480.2 68415.m00308 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains Pfam
profile PF03141: Putative methyltransferase;
non-consensus GA donor site at exon 4
Length = 595
Score = 31.1 bits (67), Expect = 4.3
Identities = 13/23 (56%), Positives = 16/23 (69%)
Query: 1086 LNAAHTYLVIFDRPFAGALHDDC 1108
+NA +T +I DR FAG LHD C
Sbjct: 481 VNARNTLPIILDRGFAGVLHDWC 503
>At2g03480.1 68415.m00307 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains Pfam
profile PF03141: Putative methyltransferase;
non-consensus GA donor site at exon 4
Length = 606
Score = 31.1 bits (67), Expect = 4.3
Identities = 13/23 (56%), Positives = 16/23 (69%)
Query: 1086 LNAAHTYLVIFDRPFAGALHDDC 1108
+NA +T +I DR FAG LHD C
Sbjct: 492 VNARNTLPIILDRGFAGVLHDWC 514
>At2g42270.1 68415.m05232 U5 small nuclear ribonucleoprotein
helicase, putative
Length = 2172
Score = 30.7 bits (66), Expect = 5.7
Identities = 29/106 (27%), Positives = 49/106 (46%), Gaps = 8/106 (7%)
Query: 530 TSLGVVDAPQYFPSIKECHAFEKRVWRNELDVPEEQLKKGMLPNARRDMLFPGFPTMRHL 589
TSL V+D Y+ K +E+++ E E+L K + RD+ + HL
Sbjct: 267 TSLNVLDIDAYWLQRKISQEYEQKIDAQECQELAEELLKILAEGNDRDV---EIKLLEHL 323
Query: 590 KYKSSHKMAKVKVFDLPARNSSV-IIELIRDKDEDSLERVANELLG 634
+++ K + VK F L R V L R +D++ ++ E+LG
Sbjct: 324 QFE---KFSLVK-FLLQNRLKVVWCTRLARGRDQEERNQIEEEMLG 365
>At1g63020.1 68414.m07117 DNA-directed RNA polymerase alpha subunit
family protein low similarity to RNA polymerase IIA
largest subunit [Trypanosoma brucei] GI:162215; contains
InterPro accession IPR000722: RNA polymerase, alpha
subunit
Length = 1453
Score = 30.7 bits (66), Expect = 5.7
Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 5/29 (17%)
Query: 99 IMDYIRWARSQPDYDPNTRHC--LYGLDA 125
IMD I W RS PD N R C +YG+DA
Sbjct: 1133 IMDMIDWGRSHPD---NIRQCCSVYGIDA 1158
>At3g46670.1 68416.m05066 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 451
Score = 29.9 bits (64), Expect = 10.0
Identities = 27/104 (25%), Positives = 46/104 (44%), Gaps = 7/104 (6%)
Query: 572 PNARRDMLFPGFPTMRHLKYKSSHKMAKVKVFDLPARN------SSVIIELIRDKDEDSL 625
P +++ L P F +R + SH + + +L SSVII + SL
Sbjct: 161 PKGQQNELVPEFHPLRCKDFPVSHWASLESMMELYRNTVDKRTASSVIINTASCLESSSL 220
Query: 626 ERVANELLGKVIWVGWPHLTRAKVISVSNE-KTRLHYVDKQNNN 668
R+ +L V +G HL + S+ E K+ + +++KQ N
Sbjct: 221 SRLQQQLQIPVYPIGPLHLVASASTSLLEENKSCIEWLNKQKKN 264
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.136 0.420
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,663,922
Number of Sequences: 28952
Number of extensions: 1070683
Number of successful extensions: 2327
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2311
Number of HSP's gapped (non-prelim): 22
length of query: 1120
length of database: 12,070,560
effective HSP length: 89
effective length of query: 1031
effective length of database: 9,493,832
effective search space: 9788140792
effective search space used: 9788140792
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 64 (29.9 bits)
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