Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000468-TA|BGIBMGA000468-PA|IPR000731|Sterol-sensing 5TM
box
(1118 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g42470.1 68414.m04897 patched family protein similar to SP|O1... 38 0.038
At4g38350.1 68417.m05422 patched family protein similar to SP|O1... 36 0.11
At5g60770.1 68418.m07625 high-affinity nitrate transporter, puta... 32 1.9
At5g65970.1 68418.m08305 seven transmembrane MLO family protein ... 32 2.5
At1g63110.3 68414.m07131 cell division cycle protein-related con... 32 2.5
At1g63110.2 68414.m07132 cell division cycle protein-related con... 32 2.5
At1g63110.1 68414.m07130 cell division cycle protein-related con... 32 2.5
At1g42560.1 68414.m04907 seven transmembrane MLO family protein ... 32 2.5
At1g08100.1 68414.m00886 high-affinity nitrate transporter (ACH2... 32 2.5
At5g22430.1 68418.m02616 expressed protein 31 3.3
At2g33670.1 68415.m04126 seven transmembrane MLO family protein ... 31 3.3
At1g08090.1 68414.m00885 high-affinity nitrate transporter (ACH1... 31 4.3
At5g58450.1 68418.m07320 expressed protein predicted proteins fr... 31 5.7
At1g33100.1 68414.m04087 MATE efflux family protein similar to r... 30 7.5
At1g16380.1 68414.m01959 cation/proton exchanger, putative (CHX1... 30 7.5
At2g16530.2 68415.m01896 3-oxo-5-alpha-steroid 4-dehydrogenase f... 30 10.0
At2g16530.1 68415.m01895 3-oxo-5-alpha-steroid 4-dehydrogenase f... 30 10.0
At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kina... 30 10.0
At1g30410.1 68414.m03717 ATP-binding cassette transport protein,... 30 10.0
>At1g42470.1 68414.m04897 patched family protein similar to SP|O15118
Niemann-Pick C1 protein precursor from Homo sapiens
(GB:AAB63982) (GI:2276463); contains Pfam profile PF02460
Patched family
Length = 1272
Score = 37.9 bits (84), Expect = 0.038
Identities = 20/92 (21%), Positives = 46/92 (50%)
Query: 869 FQFYDLQRTLASGTIVALLLSAALGFIVLIPATLNFVTSVCALTAMIFSSTVTIAVLVIN 928
+ F++ + ++ L ++ A F+V + T +F +S L + + V+ +
Sbjct: 1075 YMFFEQYLDIWKTALINLSIAIAAVFVVCLIITCSFWSSAIILLVIAMIIIDLLGVMAVF 1134
Query: 929 NWKLNILESVAISTSAGLAVDFSLHYALSYTN 960
+ +LN L V + S G+AV+F +H ++++
Sbjct: 1135 HIQLNALSVVNLIMSVGIAVEFCVHITHAFSH 1166
Score = 34.7 bits (76), Expect = 0.35
Identities = 18/62 (29%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Query: 516 TIFITTLTTAVAFYASYVSYIPAINCFSVFAGTAVLVNFVLMMTWLPISVFIVDVKLYAK 575
+I + +L +AF +PA+ FS+FA AVL++F+L +T +++ + D +
Sbjct: 708 SITLASLAEILAFAVGAFIKMPAVRVFSMFAALAVLLDFLLQITAF-VALIVFDFRRTED 766
Query: 576 KK 577
K+
Sbjct: 767 KR 768
>At4g38350.1 68417.m05422 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (PID:g2276463); contains Pfam profile PF02460
Patched family
Length = 1064
Score = 36.3 bits (80), Expect = 0.11
Identities = 36/165 (21%), Positives = 79/165 (47%), Gaps = 6/165 (3%)
Query: 418 LFLFAAITFSLGIAYFFYVYVFNLHFFPFMNLLVIVIVLGIGADDAFIYVKIWK-MIGKQ 476
L +F I+ +LG A FY + + ++ +V+V++ +G+ F + + +I +
Sbjct: 563 LVMFVYISVTLGDAPQFYTFYISSKVLLGLSGVVLVLLSVLGSVGVFSALGVKSTLIIME 622
Query: 477 LIKDNVVYQENGVLDINISSAGETILVQILEETMRHSIV----TIFITTLTTAVAFYASY 532
+I V+ + I + + LE+ + ++V +I + +L+ +AF
Sbjct: 623 VIPFLVLAVGVDNMCILVHAVKRQPREVSLEQRISSALVEVGPSITLASLSEVLAFAVGA 682
Query: 533 VSYIPAINCFSVFAGTAVLVNFVLMMTWLPISVFIVDVKLYAKKK 577
+PA FS+FA A++++F L +T +++ + D K A +
Sbjct: 683 FVPMPACRIFSMFAALAIMLDFFLQITAF-VALIVFDCKRSADNR 726
Score = 34.7 bits (76), Expect = 0.35
Identities = 23/105 (21%), Positives = 46/105 (43%), Gaps = 4/105 (3%)
Query: 931 KLNILESVAISTSAGLAVDFSLHYALSYTNASGNKSARVKYXXXXXXXXXXXXXXXXGFA 990
+LN + V + S G+AV+F +H + ++ +SG++ R +
Sbjct: 939 QLNAVSVVNLIMSIGIAVEFCVHISHAFLMSSGDREHRAREALETMGASVFSGITLTKLV 998
Query: 991 GVFLL---RSNLLPYSQIGAFLVLIMTVSWTYATFFLCSLLHIVG 1032
GV +L RS + +L L++ + + + FL +L + G
Sbjct: 999 GVIVLCFARSEIFVVYYFQMYLALVI-IGFLHGLVFLPVILSLAG 1042
>At5g60770.1 68418.m07625 high-affinity nitrate transporter,
putative strong similarity to trans-membrane nitrate
transporter protein AtNRT2:1 [Arabidopsis thaliana]
GI:3747058, high-affinity nitrate transporter ACH1
[Arabidopsis thaliana] GI:3608362
Length = 527
Score = 32.3 bits (70), Expect = 1.9
Identities = 21/57 (36%), Positives = 31/57 (54%), Gaps = 9/57 (15%)
Query: 373 IPAIDFGLKNALFDIWIIEDTWLIALGGIFVFACVWIYTRSLILTLFLFAAITFSLG 429
I A FG++ L+ +WII+ LGG F C+W+ + + T +F I FSLG
Sbjct: 341 IAARRFGMRGRLWTLWIIQ-----TLGGFF---CLWLGRATTLPTAVVF-MILFSLG 388
>At5g65970.1 68418.m08305 seven transmembrane MLO family protein /
MLO-like protein 10 (MLO10) identical to membrane
protein Mlo10 [Arabidopsis thaliana]
gi|14091590|gb|AAK53803; similar to MLO protein
SWISS-PROT:P93766, NCBI_gi:1877221 [Hordeum
vulgare][Barley]
Length = 569
Score = 31.9 bits (69), Expect = 2.5
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
Query: 408 WIYTRSLILTLFLFAAITFSLGIAYFFYV-YVFNLH--FFPFMNLLVIVIVLGIGA 460
W L+L L FA + + YFF+V Y F L F L+++ + LG+GA
Sbjct: 363 WFNRPQLLLHLLHFALFQNAFQLTYFFWVWYSFGLKSCFHTDFKLVIVKLSLGVGA 418
>At1g63110.3 68414.m07131 cell division cycle protein-related
contains 9 transmembrane domains; similar to PIG-U
(GI:27372215) [Rattus norvegicus]; similar to Cell
division cycle protein 91-like 1 (CDC91-like 1 protein)
(PIG-U) (Swiss-Prot:Q9H490) [Homo sapiens]
Length = 407
Score = 31.9 bits (69), Expect = 2.5
Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
Query: 403 VFACVWIYT---RSLILTLFLFAAITFSLGIAYFFYVYVFNLHFFPFMNLLVIVIVLGIG 459
+ +CV + T +L + L LF A+T + +A F V +L +P + I+ +LG G
Sbjct: 98 IVSCVGLSTSPIENLAVILALFGAVTRRVPLAAFGLVIATHLSLYPATLTIPIIFLLGYG 157
Query: 460 AD 461
D
Sbjct: 158 LD 159
>At1g63110.2 68414.m07132 cell division cycle protein-related
contains 9 transmembrane domains; similar to PIG-U
(GI:27372215) [Rattus norvegicus]; similar to Cell
division cycle protein 91-like 1 (CDC91-like 1 protein)
(PIG-U) (Swiss-Prot:Q9H490) [Homo sapiens]
Length = 397
Score = 31.9 bits (69), Expect = 2.5
Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
Query: 403 VFACVWIYT---RSLILTLFLFAAITFSLGIAYFFYVYVFNLHFFPFMNLLVIVIVLGIG 459
+ +CV + T +L + L LF A+T + +A F V +L +P + I+ +LG G
Sbjct: 88 IVSCVGLSTSPIENLAVILALFGAVTRRVPLAAFGLVIATHLSLYPATLTIPIIFLLGYG 147
Query: 460 AD 461
D
Sbjct: 148 LD 149
>At1g63110.1 68414.m07130 cell division cycle protein-related
contains 9 transmembrane domains; similar to PIG-U
(GI:27372215) [Rattus norvegicus]; similar to Cell
division cycle protein 91-like 1 (CDC91-like 1 protein)
(PIG-U) (Swiss-Prot:Q9H490) [Homo sapiens]
Length = 469
Score = 31.9 bits (69), Expect = 2.5
Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
Query: 403 VFACVWIYT---RSLILTLFLFAAITFSLGIAYFFYVYVFNLHFFPFMNLLVIVIVLGIG 459
+ +CV + T +L + L LF A+T + +A F V +L +P + I+ +LG G
Sbjct: 160 IVSCVGLSTSPIENLAVILALFGAVTRRVPLAAFGLVIATHLSLYPATLTIPIIFLLGYG 219
Query: 460 AD 461
D
Sbjct: 220 LD 221
>At1g42560.1 68414.m04907 seven transmembrane MLO family protein /
MLO-like protein 9 (MLO9) nearly identical to membrane
protein Mlo9 [Arabidopsis thaliana] GI:14091588; similar
to MLO protein SWISS-PROT:P93766, NCBI_gi:1877221
[Hordeum vulgare][Barley]
Length = 467
Score = 31.9 bits (69), Expect = 2.5
Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
Query: 408 WIYTRSLILTLFLFAAITFSLGIAYFFYV-YVFNLH--FFPFMNLLVIVIVLGIGADDAF 464
W L+L + F + I YFF++ Y F L F L++I + LG+G
Sbjct: 357 WFEKPDLVLHMIHFVLFQNAFEITYFFWIWYEFGLRSCFHHHFGLIIIRVCLGVGVQFLC 416
Query: 465 IYVKI 469
Y+ +
Sbjct: 417 SYITL 421
>At1g08100.1 68414.m00886 high-affinity nitrate transporter (ACH2)
identical to high-affinity nitrate transporter ACH2
[Arabidopsis thaliana] GI:3608364
Length = 522
Score = 31.9 bits (69), Expect = 2.5
Identities = 19/57 (33%), Positives = 33/57 (57%), Gaps = 9/57 (15%)
Query: 373 IPAIDFGLKNALFDIWIIEDTWLIALGGIFVFACVWIYTRSLILTLFLFAAITFSLG 429
I A FG++ L+ +WII+ + GG+F CVW+ + ++T + + + FSLG
Sbjct: 339 IAAKRFGMRGRLWTLWIIQTS-----GGLF---CVWLGRANTLVTA-VVSMVLFSLG 386
>At5g22430.1 68418.m02616 expressed protein
Length = 172
Score = 31.5 bits (68), Expect = 3.3
Identities = 14/41 (34%), Positives = 24/41 (58%)
Query: 260 ALISGKDSCVNLNKEDVKKAVTILKKCAGYFHNFTLMAACD 300
+L+SGK SC++ +++ + +L KC G T +AA D
Sbjct: 27 SLVSGKISCLDCHRDFDFSGIKVLLKCDGEKKQITAVAAAD 67
>At2g33670.1 68415.m04126 seven transmembrane MLO family protein /
MLO-like protein 5 (MLO5) identical to MLO-like protein
5 (AtMlo5) [Arabidopsis thaliana] SWISS-PROT:O22815;
similar to MLO protein SWISS-PROT:P93766,
NCBI_gi:1877221 [Hordeum vulgare][Barley]
Length = 501
Score = 31.5 bits (68), Expect = 3.3
Identities = 18/65 (27%), Positives = 30/65 (46%), Gaps = 3/65 (4%)
Query: 408 WIYTRSLILTLFLFAAITFSLGIAYFFYV-YVFNLH--FFPFMNLLVIVIVLGIGADDAF 464
W +L+L + F + I YFF++ Y F L F L++I + LG+G
Sbjct: 350 WFSRPALVLHIIHFILFQNAFEITYFFWIWYEFGLRSCFHHHFALIIIRVALGVGVQFLC 409
Query: 465 IYVKI 469
Y+ +
Sbjct: 410 SYITL 414
>At1g08090.1 68414.m00885 high-affinity nitrate transporter (ACH1)
identical to trans-membrane nitrate transporter protein
AtNRT2:1 [Arabidopsis thaliana] GI:3747058,
high-affinity nitrate transporter ACH1 [Arabidopsis
thaliana] GI:3608362
Length = 530
Score = 31.1 bits (67), Expect = 4.3
Identities = 17/52 (32%), Positives = 30/52 (57%), Gaps = 9/52 (17%)
Query: 378 FGLKNALFDIWIIEDTWLIALGGIFVFACVWIYTRSLILTLFLFAAITFSLG 429
FG++ L+ +WII+ GG+F CVW+ + ++T + A + FS+G
Sbjct: 346 FGMRGRLWTLWIIQ-----TAGGLF---CVWLGRANTLVTA-VVAMVLFSMG 388
>At5g58450.1 68418.m07320 expressed protein predicted proteins from
D.melanogaster, C.elegans and S.pombe
Length = 1065
Score = 30.7 bits (66), Expect = 5.7
Identities = 29/122 (23%), Positives = 52/122 (42%), Gaps = 8/122 (6%)
Query: 743 CFIESFKMYMSRRCIDNIDKINRSPCCETSKFPYKKEVFDFCIIKAMESLYQTPRELFMP 802
C +E ++ + DNID+I+ + E +E+FD I A + + + R+
Sbjct: 337 CLLEDDSIW---KYFDNIDQIHPTKHIECKFSHLTEEMFDSRISSASDLVQKLQRDAENS 393
Query: 803 GVAGPKF--LRSAKPPIIAALVVE---FESIVPYSLSYSEMNKFFTEVEKWTQTELENAP 857
+ GP L K + E ES++ Y L + + + ++VE + Q N
Sbjct: 394 NLRGPYLAELEIEKRKFLFGKKNEDKLLESLLQYFLKFGHLACYASDVEAYLQVLSPNKK 453
Query: 858 AG 859
AG
Sbjct: 454 AG 455
>At1g33100.1 68414.m04087 MATE efflux family protein similar to
ripening regulated protein DDTFR18 [Lycopersicon
esculentum] GI:12231296; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 475
Score = 30.3 bits (65), Expect = 7.5
Identities = 25/129 (19%), Positives = 61/129 (47%), Gaps = 7/129 (5%)
Query: 489 VLDINISSAGETILVQILEETMRHSIVTIFITTLTTAVAFYASYVSYIPAINCFSVFAGT 548
V+ IN S L+ ++ I++ ++T ++ + + S++ + N A T
Sbjct: 160 VIGINFSFVPSFTCQMFLQAQSKNKIIS-YVTAVSLGLHVFFSWL-LVAHFNFGITGAMT 217
Query: 549 AVLVNFVLMMTWLPISVFIVDVKLYAKKKTFLDLVHKNVNEMWENIRIVLNRFTIICVTK 608
++L+ F WLPI V ++ V K T+ ++W +++ L+ ++C+
Sbjct: 218 SMLIAF-----WLPIIVQLLYVTCGGCKDTWRGFSMLAFKDLWPVLKLSLSSGGMLCLEL 272
Query: 609 FHIAFVVVM 617
++ + +V++
Sbjct: 273 WYNSVLVLL 281
>At1g16380.1 68414.m01959 cation/proton exchanger, putative (CHX1)
monovalent cation:proton antiporter family 2 (CPA2)
member, PMID:11500563
Length = 785
Score = 30.3 bits (65), Expect = 7.5
Identities = 30/157 (19%), Positives = 64/157 (40%), Gaps = 3/157 (1%)
Query: 877 TLASGTIVALLLSAALGFIV-LIPATLNFVTSVCALTAMIFSSTVTIAVLVINNWKLNIL 935
TL S I ++ L F++ + +F+T A + ++ + + I +WKL+
Sbjct: 115 TLGSLVISGIIWLPFLWFLIRFMQIKGDFLTFYLAFLITLSNTAAPVVIRSIIDWKLHTS 174
Query: 936 ESVAISTSAGLAVDFS--LHYALSYTNASGNKSARVKYXXXXXXXXXXXXXXXXGFAGVF 993
E ++ S GL ++ + Y + + SG +A + +
Sbjct: 175 EIGRLAISCGLFIEITNIFIYTIVLSFISGTMTADIFIYSFATGVIILTNRFLASWLPKR 234
Query: 994 LLRSNLLPYSQIGAFLVLIMTVSWTYATFFLCSLLHI 1030
+ L ++ AF++LI+ ++ T + L S L +
Sbjct: 235 NPKEKYLSKAETLAFIILILIIALTIESSNLNSTLFV 271
>At2g16530.2 68415.m01896 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to Arabidopsis thaliana DET2
[gi:1280611]; contains Pfam 3-oxo-5-alpha-steroid
4-dehydrogenase domain PF02544
Length = 342
Score = 29.9 bits (64), Expect = 10.0
Identities = 16/47 (34%), Positives = 29/47 (61%), Gaps = 2/47 (4%)
Query: 8 FIVRHSYLVLFTVSILSIVLTVIPLILHELPSFSDPIVGFETRGTTL 54
++VR +++ ++ VSIL +V+ IP +L SF + ++ F RG L
Sbjct: 8 WLVRGAWITVWIVSILPLVIASIP--TSKLNSFRELVLSFAGRGKIL 52
>At2g16530.1 68415.m01895 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to Arabidopsis thaliana DET2
[gi:1280611]; contains Pfam 3-oxo-5-alpha-steroid
4-dehydrogenase domain PF02544
Length = 343
Score = 29.9 bits (64), Expect = 10.0
Identities = 16/47 (34%), Positives = 29/47 (61%), Gaps = 2/47 (4%)
Query: 8 FIVRHSYLVLFTVSILSIVLTVIPLILHELPSFSDPIVGFETRGTTL 54
++VR +++ ++ VSIL +V+ IP +L SF + ++ F RG L
Sbjct: 8 WLVRGAWITVWIVSILPLVIASIP--TSKLNSFRELVLSFAGRGKIL 52
>At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kinase
family protein low similarity to SP|Q9Z1T6 FYVE
finger-containing phosphoinositide kinase (EC 2.7.1.68)
(1- phosphatidylinositol-4-phosphate kinase) (PIP5K)
(PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam
profile PF01504: Phosphatidylinositol-4-phosphate
5-Kinase
Length = 1456
Score = 29.9 bits (64), Expect = 10.0
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Query: 166 KQWLSLKNMQRIADKPINLNYASGTCGPPISDYAHLVITNTDNSSLLSY 214
K+ +LK+M + P + G G PIS ++ L N DN LL Y
Sbjct: 233 KKHAALKHMATKYEHP-RIMLVEGVLGHPISGFSSLQSVNQDNEYLLKY 280
>At1g30410.1 68414.m03717 ATP-binding cassette transport protein,
putative similar to MgATP-energized glutathione
S-conjugate pump [Arabidopsis thaliana] GI:2909781;
contains Pfam profiles PF00005: ABC transporter,
PF00664: ABC transporter transmembrane region
Length = 1495
Score = 29.9 bits (64), Expect = 10.0
Identities = 21/82 (25%), Positives = 42/82 (51%), Gaps = 3/82 (3%)
Query: 462 DAFIYVKIWKMIG---KQLIKDNVVYQENGVLDINISSAGETILVQILEETMRHSIVTIF 518
+AF + + +IG KQ +K+ Y GVL + ++ A LV L+ ++ + + +F
Sbjct: 118 EAFAWFSMLVLIGLETKQYVKEFRWYVRFGVLYVLVADAVLLDLVLPLKNSINRTALYLF 177
Query: 519 ITTLTTAVAFYASYVSYIPAIN 540
I++ + F + YIP ++
Sbjct: 178 ISSRCSQALFGILLLIYIPELD 199
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.324 0.138 0.422
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,991,882
Number of Sequences: 28952
Number of extensions: 1031915
Number of successful extensions: 3272
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 3256
Number of HSP's gapped (non-prelim): 27
length of query: 1118
length of database: 12,070,560
effective HSP length: 89
effective length of query: 1029
effective length of database: 9,493,832
effective search space: 9769153128
effective search space used: 9769153128
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.6 bits)
S2: 64 (29.9 bits)
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