Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000464-TA|BGIBMGA000464-PA|IPR007209|Possible
metal-binding region in RNase L inhibitor, RLI, IPR007177|Protein of
unknown function DUF367
         (240 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g10070.2 68418.m01167 RNase L inhibitor protein-related conta...   202   2e-52
At5g10070.1 68418.m01166 RNase L inhibitor protein-related conta...   202   2e-52
At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kina...    31   0.92 
At3g45750.1 68416.m04944 expressed protein                             30   1.2  
At3g53960.1 68416.m05961 proton-dependent oligopeptide transport...    29   2.8  
At2g31530.1 68415.m03852 secY family protein low similarity to S...    27   8.6  
At1g58230.1 68414.m06618 WD-40 repeat family protein / beige-rel...    27   8.6  

>At5g10070.2 68418.m01167 RNase L inhibitor protein-related contains
           Pfam profiles PF04034: Domain of unknown function
           (DUF367), PF04068: Possible metal-binding domain in
           RNase L inhibitor, RLI
          Length = 266

 Score =  202 bits (492), Expect = 2e-52
 Identities = 89/179 (49%), Positives = 125/179 (69%), Gaps = 4/179 (2%)

Query: 54  VSMWDLNHCDPKKCSGRKLLRHNLIKNLKLGHRFQGLVLSPVGTQCVSPNDKNIIETFGL 113
           ++MWD   CD K+C+GRKL R NL+K L++   F G+VLSPVG QCVS  D ++I+  GL
Sbjct: 46  LAMWDFGQCDAKRCTGRKLARFNLLKELRVNTGFGGVVLSPVGRQCVSREDYDLIKRKGL 105

Query: 114 AVIDCSWAKIDETPFSRMKSPHPRLLPFLVAANPINYGKPYQLSCVEALAAAMFITGHKS 173
           AV+DCSWA++ + PF++++   PRLLP+LVAANP+NYG+P +LSCVEAL+AA+ + G K 
Sbjct: 106 AVVDCSWARLTDVPFAKLRCTAPRLLPWLVAANPVNYGRPCELSCVEALSAALILCGEKE 165

Query: 174 EAKFYLSKFSWGHSFLELNSDALDLYAVCTDSKSVLEAQATFLESVKNEQDDRPMWPPS 232
            A+  L KF WGH+FL LN D L  Y+ C +S  ++  Q ++L     +Q   P  PP+
Sbjct: 166 TAELLLGKFKWGHAFLSLNKDILKEYSKCENSAEIISVQNSWL----TQQTQIPKQPPA 220


>At5g10070.1 68418.m01166 RNase L inhibitor protein-related contains
           Pfam profiles PF04034: Domain of unknown function
           (DUF367), PF04068: Possible metal-binding domain in
           RNase L inhibitor, RLI
          Length = 264

 Score =  202 bits (492), Expect = 2e-52
 Identities = 89/179 (49%), Positives = 125/179 (69%), Gaps = 4/179 (2%)

Query: 54  VSMWDLNHCDPKKCSGRKLLRHNLIKNLKLGHRFQGLVLSPVGTQCVSPNDKNIIETFGL 113
           ++MWD   CD K+C+GRKL R NL+K L++   F G+VLSPVG QCVS  D ++I+  GL
Sbjct: 46  LAMWDFGQCDAKRCTGRKLARFNLLKELRVNTGFGGVVLSPVGRQCVSREDYDLIKRKGL 105

Query: 114 AVIDCSWAKIDETPFSRMKSPHPRLLPFLVAANPINYGKPYQLSCVEALAAAMFITGHKS 173
           AV+DCSWA++ + PF++++   PRLLP+LVAANP+NYG+P +LSCVEAL+AA+ + G K 
Sbjct: 106 AVVDCSWARLTDVPFAKLRCTAPRLLPWLVAANPVNYGRPCELSCVEALSAALILCGEKE 165

Query: 174 EAKFYLSKFSWGHSFLELNSDALDLYAVCTDSKSVLEAQATFLESVKNEQDDRPMWPPS 232
            A+  L KF WGH+FL LN D L  Y+ C +S  ++  Q ++L     +Q   P  PP+
Sbjct: 166 TAELLLGKFKWGHAFLSLNKDILKEYSKCENSAEIISVQNSWL----TQQTQIPKQPPA 220


>At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kinase
           family protein low similarity to SP|Q9Z1T6 FYVE
           finger-containing phosphoinositide kinase (EC 2.7.1.68)
           (1- phosphatidylinositol-4-phosphate kinase) (PIP5K)
           (PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam
           profile PF01504: Phosphatidylinositol-4-phosphate
           5-Kinase
          Length = 1456

 Score = 30.7 bits (66), Expect = 0.92
 Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 2/47 (4%)

Query: 193 SDALDLYAVCTDSKSVLEAQATFLESVKNEQDDRPMWPPSESDSASD 239
           SD LD+    +   SV   Q  FL   +++ DD P+W P E ++  D
Sbjct: 62  SDILDILDEKSKENSVENVQ--FLSDREDDSDDVPVWEPPEPENPED 106


>At3g45750.1 68416.m04944 expressed protein
          Length = 682

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 14/35 (40%), Positives = 21/35 (60%)

Query: 191 LNSDALDLYAVCTDSKSVLEAQATFLESVKNEQDD 225
           LN+ ALD+Y    +S  VLEA  +F+  + + Q D
Sbjct: 71  LNTMALDIYGKSEESSPVLEAYGSFVMDMYSSQSD 105


>At3g53960.1 68416.m05961 proton-dependent oligopeptide transport
           (POT) family protein contains Pfam profile: PF00854 POT
           family
          Length = 602

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 15/51 (29%), Positives = 24/51 (47%)

Query: 139 LPFLVAANPINYGKPYQLSCVEALAAAMFITGHKSEAKFYLSKFSWGHSFL 189
           + F +A   I+ G       +E+  A  F  GH  E K  +S F+W ++ L
Sbjct: 152 IAFFIAIYLISIGTGGHKPSLESFGADQFEDGHPEERKMKMSYFNWWNAGL 202


>At2g31530.1 68415.m03852 secY family protein low similarity to
           SP|P31159 Preprotein translocase secY subunit
           {Synechococcus sp}; contains Pfam profile PF00344:
           eubacterial secY protein
          Length = 556

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 21/81 (25%), Positives = 39/81 (48%), Gaps = 3/81 (3%)

Query: 148 INYGKPYQLSCVEALAAAMFITGHKSEAKFYLSKF-SWGHSFLELNSDALDLYAVCTDSK 206
           +N  +  Q   ++     + +   KS +  Y  KF     S  E+++DALD+  +  DS+
Sbjct: 27  LNMSRTRQGHSIQMNRRHLLMKERKSFSINYSDKFRDDSMSSEEMHTDALDVEIIPPDSQ 86

Query: 207 SVLEAQATFLESVKNEQDDRP 227
            +  +Q + + +    QDDRP
Sbjct: 87  DIRNSQNSAVSNTL--QDDRP 105


>At1g58230.1 68414.m06618 WD-40 repeat family protein /
           beige-related contains Pfam PF00400: WD domain, G-beta
           repeat; similar to Lipopolysaccharide-responsive and
           beige-like anchor protein (CDC4-like protein)
           (Beige-like protein) (SP:P50851) [Homo sapiens}
          Length = 1280

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 5/75 (6%)

Query: 105 KNIIETFGLAVIDCSWAKIDETPFSRMKSPHPRLLPFLVAANPINYGKPYQLSCVEALAA 164
           +N+ +   ++ I+   A   +TP    +  HPR  P +  A+P+ Y  P  ++    L A
Sbjct: 869 ENMEDQLQISAIEDQIANFGQTPIQIFRKKHPRRGPPIPIAHPL-YFAPASINLSSILPA 927

Query: 165 AMFITGHKSEAKFYL 179
               T H   A  Y+
Sbjct: 928 ----TTHSPSAVLYV 938


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.317    0.132    0.406 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,632,535
Number of Sequences: 28952
Number of extensions: 215580
Number of successful extensions: 426
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 422
Number of HSP's gapped (non-prelim): 7
length of query: 240
length of database: 12,070,560
effective HSP length: 79
effective length of query: 161
effective length of database: 9,783,352
effective search space: 1575119672
effective search space used: 1575119672
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 58 (27.5 bits)