BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000464-TA|BGIBMGA000464-PA|IPR007209|Possible
metal-binding region in RNase L inhibitor, RLI, IPR007177|Protein of
unknown function DUF367
(240 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g10070.2 68418.m01167 RNase L inhibitor protein-related conta... 202 2e-52
At5g10070.1 68418.m01166 RNase L inhibitor protein-related conta... 202 2e-52
At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kina... 31 0.92
At3g45750.1 68416.m04944 expressed protein 30 1.2
At3g53960.1 68416.m05961 proton-dependent oligopeptide transport... 29 2.8
At2g31530.1 68415.m03852 secY family protein low similarity to S... 27 8.6
At1g58230.1 68414.m06618 WD-40 repeat family protein / beige-rel... 27 8.6
>At5g10070.2 68418.m01167 RNase L inhibitor protein-related contains
Pfam profiles PF04034: Domain of unknown function
(DUF367), PF04068: Possible metal-binding domain in
RNase L inhibitor, RLI
Length = 266
Score = 202 bits (492), Expect = 2e-52
Identities = 89/179 (49%), Positives = 125/179 (69%), Gaps = 4/179 (2%)
Query: 54 VSMWDLNHCDPKKCSGRKLLRHNLIKNLKLGHRFQGLVLSPVGTQCVSPNDKNIIETFGL 113
++MWD CD K+C+GRKL R NL+K L++ F G+VLSPVG QCVS D ++I+ GL
Sbjct: 46 LAMWDFGQCDAKRCTGRKLARFNLLKELRVNTGFGGVVLSPVGRQCVSREDYDLIKRKGL 105
Query: 114 AVIDCSWAKIDETPFSRMKSPHPRLLPFLVAANPINYGKPYQLSCVEALAAAMFITGHKS 173
AV+DCSWA++ + PF++++ PRLLP+LVAANP+NYG+P +LSCVEAL+AA+ + G K
Sbjct: 106 AVVDCSWARLTDVPFAKLRCTAPRLLPWLVAANPVNYGRPCELSCVEALSAALILCGEKE 165
Query: 174 EAKFYLSKFSWGHSFLELNSDALDLYAVCTDSKSVLEAQATFLESVKNEQDDRPMWPPS 232
A+ L KF WGH+FL LN D L Y+ C +S ++ Q ++L +Q P PP+
Sbjct: 166 TAELLLGKFKWGHAFLSLNKDILKEYSKCENSAEIISVQNSWL----TQQTQIPKQPPA 220
>At5g10070.1 68418.m01166 RNase L inhibitor protein-related contains
Pfam profiles PF04034: Domain of unknown function
(DUF367), PF04068: Possible metal-binding domain in
RNase L inhibitor, RLI
Length = 264
Score = 202 bits (492), Expect = 2e-52
Identities = 89/179 (49%), Positives = 125/179 (69%), Gaps = 4/179 (2%)
Query: 54 VSMWDLNHCDPKKCSGRKLLRHNLIKNLKLGHRFQGLVLSPVGTQCVSPNDKNIIETFGL 113
++MWD CD K+C+GRKL R NL+K L++ F G+VLSPVG QCVS D ++I+ GL
Sbjct: 46 LAMWDFGQCDAKRCTGRKLARFNLLKELRVNTGFGGVVLSPVGRQCVSREDYDLIKRKGL 105
Query: 114 AVIDCSWAKIDETPFSRMKSPHPRLLPFLVAANPINYGKPYQLSCVEALAAAMFITGHKS 173
AV+DCSWA++ + PF++++ PRLLP+LVAANP+NYG+P +LSCVEAL+AA+ + G K
Sbjct: 106 AVVDCSWARLTDVPFAKLRCTAPRLLPWLVAANPVNYGRPCELSCVEALSAALILCGEKE 165
Query: 174 EAKFYLSKFSWGHSFLELNSDALDLYAVCTDSKSVLEAQATFLESVKNEQDDRPMWPPS 232
A+ L KF WGH+FL LN D L Y+ C +S ++ Q ++L +Q P PP+
Sbjct: 166 TAELLLGKFKWGHAFLSLNKDILKEYSKCENSAEIISVQNSWL----TQQTQIPKQPPA 220
>At1g34260.1 68414.m04252 phosphatidylinositol-4-phosphate 5-kinase
family protein low similarity to SP|Q9Z1T6 FYVE
finger-containing phosphoinositide kinase (EC 2.7.1.68)
(1- phosphatidylinositol-4-phosphate kinase) (PIP5K)
(PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam
profile PF01504: Phosphatidylinositol-4-phosphate
5-Kinase
Length = 1456
Score = 30.7 bits (66), Expect = 0.92
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Query: 193 SDALDLYAVCTDSKSVLEAQATFLESVKNEQDDRPMWPPSESDSASD 239
SD LD+ + SV Q FL +++ DD P+W P E ++ D
Sbjct: 62 SDILDILDEKSKENSVENVQ--FLSDREDDSDDVPVWEPPEPENPED 106
>At3g45750.1 68416.m04944 expressed protein
Length = 682
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/35 (40%), Positives = 21/35 (60%)
Query: 191 LNSDALDLYAVCTDSKSVLEAQATFLESVKNEQDD 225
LN+ ALD+Y +S VLEA +F+ + + Q D
Sbjct: 71 LNTMALDIYGKSEESSPVLEAYGSFVMDMYSSQSD 105
>At3g53960.1 68416.m05961 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 602
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/51 (29%), Positives = 24/51 (47%)
Query: 139 LPFLVAANPINYGKPYQLSCVEALAAAMFITGHKSEAKFYLSKFSWGHSFL 189
+ F +A I+ G +E+ A F GH E K +S F+W ++ L
Sbjct: 152 IAFFIAIYLISIGTGGHKPSLESFGADQFEDGHPEERKMKMSYFNWWNAGL 202
>At2g31530.1 68415.m03852 secY family protein low similarity to
SP|P31159 Preprotein translocase secY subunit
{Synechococcus sp}; contains Pfam profile PF00344:
eubacterial secY protein
Length = 556
Score = 27.5 bits (58), Expect = 8.6
Identities = 21/81 (25%), Positives = 39/81 (48%), Gaps = 3/81 (3%)
Query: 148 INYGKPYQLSCVEALAAAMFITGHKSEAKFYLSKF-SWGHSFLELNSDALDLYAVCTDSK 206
+N + Q ++ + + KS + Y KF S E+++DALD+ + DS+
Sbjct: 27 LNMSRTRQGHSIQMNRRHLLMKERKSFSINYSDKFRDDSMSSEEMHTDALDVEIIPPDSQ 86
Query: 207 SVLEAQATFLESVKNEQDDRP 227
+ +Q + + + QDDRP
Sbjct: 87 DIRNSQNSAVSNTL--QDDRP 105
>At1g58230.1 68414.m06618 WD-40 repeat family protein /
beige-related contains Pfam PF00400: WD domain, G-beta
repeat; similar to Lipopolysaccharide-responsive and
beige-like anchor protein (CDC4-like protein)
(Beige-like protein) (SP:P50851) [Homo sapiens}
Length = 1280
Score = 27.5 bits (58), Expect = 8.6
Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 5/75 (6%)
Query: 105 KNIIETFGLAVIDCSWAKIDETPFSRMKSPHPRLLPFLVAANPINYGKPYQLSCVEALAA 164
+N+ + ++ I+ A +TP + HPR P + A+P+ Y P ++ L A
Sbjct: 869 ENMEDQLQISAIEDQIANFGQTPIQIFRKKHPRRGPPIPIAHPL-YFAPASINLSSILPA 927
Query: 165 AMFITGHKSEAKFYL 179
T H A Y+
Sbjct: 928 ----TTHSPSAVLYV 938
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.132 0.406
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,632,535
Number of Sequences: 28952
Number of extensions: 215580
Number of successful extensions: 426
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 422
Number of HSP's gapped (non-prelim): 7
length of query: 240
length of database: 12,070,560
effective HSP length: 79
effective length of query: 161
effective length of database: 9,783,352
effective search space: 1575119672
effective search space used: 1575119672
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 58 (27.5 bits)
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