SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000454-TA|BGIBMGA000454-PA|IPR001478|PDZ/DHR/GLGF,
IPR000008|C2 calcium-dependent membrane targeting, IPR000198|RhoGAP,
IPR008936|Rho GTPase activation protein, IPR008973|C2
calcium/lipid-binding region, CaLB
         (1292 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar...    73   3e-13
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    67   2e-11
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    62   4e-10
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar...    62   6e-10
SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |...    36   0.037
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    36   0.037
SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar...    31   1.0  
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy...    29   3.2  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    29   3.2  
SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc...    29   4.2  
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ...    29   5.6  
SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces pom...    29   5.6  

>SPBC23G7.08c |rga7||GTPase activating protein
            Rga7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 72.9 bits (171), Expect = 3e-13
 Identities = 61/208 (29%), Positives = 98/208 (47%), Gaps = 18/208 (8%)

Query: 885  RPTQGRVSPPP-------LFGSELEAVVAREIRPPNAPPVPLXXXXXXXXXXXXGLDIIG 937
            RPT   VSP P       LFG+ L+A++ RE    N P + +            GL++ G
Sbjct: 487  RPTS--VSPQPSSPTKSLLFGARLDAIILRE--HSNIPNIVMQCTSQVENF---GLNLQG 539

Query: 938  LYRLCGAASKKRVLREGFERNARTVELTPEAVP-DINVITGVLKDYLRELPQPLFSRCLF 996
            +YR+  ++++  +LR  FE N      TPE    D++ +  +LK + REL +PL      
Sbjct: 540  IYRVPSSSARVNMLRSQFENNPLLQLHTPEDYENDVHAVADLLKIFFRELREPLIPDNHQ 599

Query: 997  QMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQH 1056
            +  +DA  V   +D       +   +  LP A  +T+  L  HLA +    D NKMS  +
Sbjct: 600  RDFIDAGNV---EDESRRRDAVHRAINDLPDANYSTIRHLTIHLAKIKENSDVNKMSTNN 656

Query: 1057 LAVALAPPLMLHSSPPGELDYQRPIHVL 1084
            LA+   P ++  ++ P    + R I +L
Sbjct: 657  LAIIWGPTIIKQATIPEISSFSRTIEIL 684


>SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 969

 Score = 66.9 bits (156), Expect = 2e-11
 Identities = 51/187 (27%), Positives = 85/187 (45%), Gaps = 13/187 (6%)

Query: 880  ELFRRRPTQGRVSPPPLFGSELEAVVAREIRPPNAPPVPLXXXXXXXXXXXXGLDIIGLY 939
            E+  R P  G    P +FG  LE  +  E        +P             GL++ G+Y
Sbjct: 763  EMPTRMPPPG--PSPTMFGRSLENQLKIE-----GSVLPQVIAMCVSCVDAHGLEVEGIY 815

Query: 940  RLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLFSRCLFQMT 999
            R+ G+AS+ RVL + FE  +  +E       D+   T VLK YL  LP+P+     ++  
Sbjct: 816  RISGSASQVRVLVDEFENGSIRME---HLTSDLFACTSVLKTYLHRLPEPVIPGTQYEEL 872

Query: 1000 LDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQHLAV 1059
            L+A  +   +  E     +  +++ L  A  +   FL+ HL  V    ++N M+ ++++ 
Sbjct: 873  LEAEKI---EKEEEKIERVVEVMKTLHPAHLSVFRFLIAHLGRVCKHAEKNLMNSKNVST 929

Query: 1060 ALAPPLM 1066
              AP LM
Sbjct: 930  VFAPTLM 936


>SPBC28E12.03 |rga4||GTPase activating protein
            Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 62.5 bits (145), Expect = 4e-10
 Identities = 44/135 (32%), Positives = 66/135 (48%), Gaps = 7/135 (5%)

Query: 932  GLDIIGLYRLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLF 991
            G+D  GLYR  GA S+ + +     RN  TV L P    DI+ +T V K YLR LP P+ 
Sbjct: 775  GMDFEGLYRKSGATSQMKKI-VALLRNEDTV-LDPSE--DISAVTSVFKQYLRNLPNPII 830

Query: 992  SRCLFQMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNK 1051
            +   +   + A       D+         +++ LP A    L  ++ HLA V A    N+
Sbjct: 831  TYDQYFPFITAANCASFQDKLDG---FIMVIKSLPPAHAEILQLIIRHLARVAAYSHANR 887

Query: 1052 MSPQHLAVALAPPLM 1066
            M+ ++LAV  +P L+
Sbjct: 888  MTSKNLAVVFSPTLI 902



 Score = 29.1 bits (62), Expect = 4.2
 Identities = 20/56 (35%), Positives = 33/56 (58%), Gaps = 5/56 (8%)

Query: 492 RRNRSLDYSSDTEATAPTRPPYYSG---LSGYRSSTLGRDITSKFN-SLPRDVRNT 543
           RR+ +++Y S +  T+P++  Y SG   LS        RD+T+K N  +PR+ RN+
Sbjct: 324 RRSSTMNYKSVSTTTSPSKYGYVSGRIALSPIHLRGALRDVTNKCNLKVPRN-RNS 378


>SPAC26A3.09c |rga2||GTPase activating protein
            Rga2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1275

 Score = 61.7 bits (143), Expect = 6e-10
 Identities = 43/133 (32%), Positives = 68/133 (51%), Gaps = 3/133 (2%)

Query: 937  GLYRLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLFSRCLF 996
            G+YRL G+AS  + L+E F        L+ +   D++VI G+LK YLR LP  L    + 
Sbjct: 1103 GIYRLSGSASTIKHLKEQFNEGVDYDLLSSDEEFDVHVIAGLLKLYLRNLPTNLLDTSMH 1162

Query: 997  QMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQH 1056
            +  L  L   +P+D      L   ++  LP    A L  LL HL  ++A +  NKM+ ++
Sbjct: 1163 K--LFELLPNVPNDSAALGEL-CDVISKLPPENFALLDSLLHHLRRIIAFEKVNKMNIRN 1219

Query: 1057 LAVALAPPLMLHS 1069
            + +  +P L + S
Sbjct: 1220 VCIVFSPTLNIPS 1232


>SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 777

 Score = 35.9 bits (79), Expect = 0.037
 Identities = 29/113 (25%), Positives = 55/113 (48%), Gaps = 4/113 (3%)

Query: 951  LREGFERNAR-TVELTPEAVPDINVITGVLKDYLRELPQPLFSRCLFQMTLDALGVCLPD 1009
            LRE    ++  T E+  +  P  +V+ GVLK Y  ELP  +     F++ + ++ +   +
Sbjct: 507  LREALNHSSSVTKEVLSQYTP--SVVIGVLKLYFLELPDSIVPSSAFEL-IRSIYMNHSN 563

Query: 1010 DREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQHLAVALA 1062
            D     RL+ +++  L R   ATL  ++ HL  ++      +    +LA +L+
Sbjct: 564  DTPYRLRLLQNLLSQLRRVNLATLSAIITHLNRLITLTPNKETFTINLANSLS 616


>SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1150

 Score = 35.9 bits (79), Expect = 0.037
 Identities = 31/143 (21%), Positives = 65/143 (45%), Gaps = 11/143 (7%)

Query: 933  LDIIGLYRLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLFS 992
            + ++G++R  G   + + L +  + +  +++   E    +     +LK +LRELP PL +
Sbjct: 877  MSVVGVFRKNGNIRRLKELSDMLDVSPDSIDYEQETPIQL---AALLKKFLRELPDPLLT 933

Query: 993  RCLFQMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVA---AQDR 1049
              LF + + +  +   +  E   R++   +  LP+  R T+  +   L  V +     D 
Sbjct: 934  FKLFGLFITSSKL---ESEEERMRVLHLTICLLPKGHRDTMEVIFGFLYWVASFSHIDDE 990

Query: 1050 --NKMSPQHLAVALAPPLMLHSS 1070
              +KM   +LA  + P ++   S
Sbjct: 991  VGSKMDIHNLATVITPNILYSKS 1013


>SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 598

 Score = 31.1 bits (67), Expect = 1.0
 Identities = 19/62 (30%), Positives = 27/62 (43%), Gaps = 1/62 (1%)

Query: 296 STWSYRPPPPVITEQPKSSATHFVPYERSYPNTLDSLAEKVHSYYPPESGSTRFGGRVPR 355
           ++W Y PPPP  T +  S+A      +       D++A      Y P S   R G  + R
Sbjct: 456 ASWHYVPPPPESTVEIVSNAPTLAMEDVQPTAASDTMALAPQEVYKP-SNENREGETITR 514

Query: 356 SG 357
           SG
Sbjct: 515 SG 516


>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 436

 Score = 29.5 bits (63), Expect = 3.2
 Identities = 11/29 (37%), Positives = 17/29 (58%)

Query: 298 WSYRPPPPVITEQPKSSATHFVPYERSYP 326
           W Y  P P++T    S+A + VP++  YP
Sbjct: 375 WGYAFPAPLLTPYEASAAFNVVPWKEVYP 403


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 29.5 bits (63), Expect = 3.2
 Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 4/54 (7%)

Query: 279 DSAALDLYYNPRPP-SDHSTWSYRPPPPVITEQPKSSATHFVPYERS---YPNT 328
           DSA+  L  N  PP S H   S+ PP P  +  P ++ T  VP   +   YP+T
Sbjct: 31  DSASPRLTPNLPPPFSVHQLQSFVPPQPPSSSSPSTTGTVAVPINGANAVYPST 84


>SPAC12B10.03 |||WD repeat protein, human WDR20
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 543

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 21/88 (23%), Positives = 33/88 (37%), Gaps = 2/88 (2%)

Query: 264 GREERSRLQL--GALSPDSAALDLYYNPRPPSDHSTWSYRPPPPVITEQPKSSATHFVPY 321
           G   RS  Q+    L P++    L++     +  S W Y  P   I E  K    H V  
Sbjct: 31  GSSSRSTPQITGSPLDPNTPVKRLFFMRINIAQFSRWDYPYPREYIEELKKIHGVHHVEE 90

Query: 322 ERSYPNTLDSLAEKVHSYYPPESGSTRF 349
            R  P+ L++        +   S +T +
Sbjct: 91  NRDVPSILENYRNSKRKSHNFSSSNTPY 118


>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
           membrane transporter Hmt1|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 830

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 23/72 (31%), Positives = 34/72 (47%), Gaps = 6/72 (8%)

Query: 101 FALQDTRHVVQLVEIVKRPGQTLGLYIREGDGGTRTDGVFISRIALESAVYNSGCLKVGD 160
           FA    + V+  +  V +PG+ + L + E  GG  T    I RI L     NSG + + D
Sbjct: 591 FAYDPRKPVLSDINFVAQPGKVIAL-VGESGGGKST----IMRILLRFFDVNSGSITIDD 645

Query: 161 -EILAVNLVDVR 171
            +I  V L  +R
Sbjct: 646 QDIRNVTLSSLR 657


>SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 286

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 13/33 (39%), Positives = 22/33 (66%)

Query: 161 EILAVNLVDVRRMSLDDVVIIMSIPRRLLLCTR 193
           +ILA+++ D R   L+D+  + ++  RLL CTR
Sbjct: 161 KILAIDINDPRAKLLNDISDVQNLMPRLLPCTR 193


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.135    0.404 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,657,997
Number of Sequences: 5004
Number of extensions: 172220
Number of successful extensions: 429
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 410
Number of HSP's gapped (non-prelim): 16
length of query: 1292
length of database: 2,362,478
effective HSP length: 81
effective length of query: 1211
effective length of database: 1,957,154
effective search space: 2370113494
effective search space used: 2370113494
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 59 (27.9 bits)

- SilkBase 1999-2023 -