BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000454-TA|BGIBMGA000454-PA|IPR001478|PDZ/DHR/GLGF, IPR000008|C2 calcium-dependent membrane targeting, IPR000198|RhoGAP, IPR008936|Rho GTPase activation protein, IPR008973|C2 calcium/lipid-binding region, CaLB (1292 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 73 3e-13 SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 67 2e-11 SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 62 4e-10 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 62 6e-10 SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |... 36 0.037 SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 36 0.037 SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 31 1.0 SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 29 3.2 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 29 3.2 SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc... 29 4.2 SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 29 5.6 SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces pom... 29 5.6 >SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 72.9 bits (171), Expect = 3e-13 Identities = 61/208 (29%), Positives = 98/208 (47%), Gaps = 18/208 (8%) Query: 885 RPTQGRVSPPP-------LFGSELEAVVAREIRPPNAPPVPLXXXXXXXXXXXXGLDIIG 937 RPT VSP P LFG+ L+A++ RE N P + + GL++ G Sbjct: 487 RPTS--VSPQPSSPTKSLLFGARLDAIILRE--HSNIPNIVMQCTSQVENF---GLNLQG 539 Query: 938 LYRLCGAASKKRVLREGFERNARTVELTPEAVP-DINVITGVLKDYLRELPQPLFSRCLF 996 +YR+ ++++ +LR FE N TPE D++ + +LK + REL +PL Sbjct: 540 IYRVPSSSARVNMLRSQFENNPLLQLHTPEDYENDVHAVADLLKIFFRELREPLIPDNHQ 599 Query: 997 QMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQH 1056 + +DA V +D + + LP A +T+ L HLA + D NKMS + Sbjct: 600 RDFIDAGNV---EDESRRRDAVHRAINDLPDANYSTIRHLTIHLAKIKENSDVNKMSTNN 656 Query: 1057 LAVALAPPLMLHSSPPGELDYQRPIHVL 1084 LA+ P ++ ++ P + R I +L Sbjct: 657 LAIIWGPTIIKQATIPEISSFSRTIEIL 684 >SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 66.9 bits (156), Expect = 2e-11 Identities = 51/187 (27%), Positives = 85/187 (45%), Gaps = 13/187 (6%) Query: 880 ELFRRRPTQGRVSPPPLFGSELEAVVAREIRPPNAPPVPLXXXXXXXXXXXXGLDIIGLY 939 E+ R P G P +FG LE + E +P GL++ G+Y Sbjct: 763 EMPTRMPPPG--PSPTMFGRSLENQLKIE-----GSVLPQVIAMCVSCVDAHGLEVEGIY 815 Query: 940 RLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLFSRCLFQMT 999 R+ G+AS+ RVL + FE + +E D+ T VLK YL LP+P+ ++ Sbjct: 816 RISGSASQVRVLVDEFENGSIRME---HLTSDLFACTSVLKTYLHRLPEPVIPGTQYEEL 872 Query: 1000 LDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQHLAV 1059 L+A + + E + +++ L A + FL+ HL V ++N M+ ++++ Sbjct: 873 LEAEKI---EKEEEKIERVVEVMKTLHPAHLSVFRFLIAHLGRVCKHAEKNLMNSKNVST 929 Query: 1060 ALAPPLM 1066 AP LM Sbjct: 930 VFAPTLM 936 >SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosaccharomyces pombe|chr 2|||Manual Length = 933 Score = 62.5 bits (145), Expect = 4e-10 Identities = 44/135 (32%), Positives = 66/135 (48%), Gaps = 7/135 (5%) Query: 932 GLDIIGLYRLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLF 991 G+D GLYR GA S+ + + RN TV L P DI+ +T V K YLR LP P+ Sbjct: 775 GMDFEGLYRKSGATSQMKKI-VALLRNEDTV-LDPSE--DISAVTSVFKQYLRNLPNPII 830 Query: 992 SRCLFQMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNK 1051 + + + A D+ +++ LP A L ++ HLA V A N+ Sbjct: 831 TYDQYFPFITAANCASFQDKLDG---FIMVIKSLPPAHAEILQLIIRHLARVAAYSHANR 887 Query: 1052 MSPQHLAVALAPPLM 1066 M+ ++LAV +P L+ Sbjct: 888 MTSKNLAVVFSPTLI 902 Score = 29.1 bits (62), Expect = 4.2 Identities = 20/56 (35%), Positives = 33/56 (58%), Gaps = 5/56 (8%) Query: 492 RRNRSLDYSSDTEATAPTRPPYYSG---LSGYRSSTLGRDITSKFN-SLPRDVRNT 543 RR+ +++Y S + T+P++ Y SG LS RD+T+K N +PR+ RN+ Sbjct: 324 RRSSTMNYKSVSTTTSPSKYGYVSGRIALSPIHLRGALRDVTNKCNLKVPRN-RNS 378 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 61.7 bits (143), Expect = 6e-10 Identities = 43/133 (32%), Positives = 68/133 (51%), Gaps = 3/133 (2%) Query: 937 GLYRLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLFSRCLF 996 G+YRL G+AS + L+E F L+ + D++VI G+LK YLR LP L + Sbjct: 1103 GIYRLSGSASTIKHLKEQFNEGVDYDLLSSDEEFDVHVIAGLLKLYLRNLPTNLLDTSMH 1162 Query: 997 QMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQH 1056 + L L +P+D L ++ LP A L LL HL ++A + NKM+ ++ Sbjct: 1163 K--LFELLPNVPNDSAALGEL-CDVISKLPPENFALLDSLLHHLRRIIAFEKVNKMNIRN 1219 Query: 1057 LAVALAPPLMLHS 1069 + + +P L + S Sbjct: 1220 VCIVFSPTLNIPS 1232 >SPAC13A11.01c |rga8|SPAC2F7.18c|GTPase activating protein Rga8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 777 Score = 35.9 bits (79), Expect = 0.037 Identities = 29/113 (25%), Positives = 55/113 (48%), Gaps = 4/113 (3%) Query: 951 LREGFERNAR-TVELTPEAVPDINVITGVLKDYLRELPQPLFSRCLFQMTLDALGVCLPD 1009 LRE ++ T E+ + P +V+ GVLK Y ELP + F++ + ++ + + Sbjct: 507 LREALNHSSSVTKEVLSQYTP--SVVIGVLKLYFLELPDSIVPSSAFEL-IRSIYMNHSN 563 Query: 1010 DREGNARLMASIVECLPRAARATLVFLLDHLALVVAAQDRNKMSPQHLAVALA 1062 D RL+ +++ L R ATL ++ HL ++ + +LA +L+ Sbjct: 564 DTPYRLRLLQNLLSQLRRVNLATLSAIITHLNRLITLTPNKETFTINLANSLS 616 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 35.9 bits (79), Expect = 0.037 Identities = 31/143 (21%), Positives = 65/143 (45%), Gaps = 11/143 (7%) Query: 933 LDIIGLYRLCGAASKKRVLREGFERNARTVELTPEAVPDINVITGVLKDYLRELPQPLFS 992 + ++G++R G + + L + + + +++ E + +LK +LRELP PL + Sbjct: 877 MSVVGVFRKNGNIRRLKELSDMLDVSPDSIDYEQETPIQL---AALLKKFLRELPDPLLT 933 Query: 993 RCLFQMTLDALGVCLPDDREGNARLMASIVECLPRAARATLVFLLDHLALVVA---AQDR 1049 LF + + + + + E R++ + LP+ R T+ + L V + D Sbjct: 934 FKLFGLFITSSKL---ESEEERMRVLHLTICLLPKGHRDTMEVIFGFLYWVASFSHIDDE 990 Query: 1050 --NKMSPQHLAVALAPPLMLHSS 1070 +KM +LA + P ++ S Sbjct: 991 VGSKMDIHNLATVITPNILYSKS 1013 >SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 31.1 bits (67), Expect = 1.0 Identities = 19/62 (30%), Positives = 27/62 (43%), Gaps = 1/62 (1%) Query: 296 STWSYRPPPPVITEQPKSSATHFVPYERSYPNTLDSLAEKVHSYYPPESGSTRFGGRVPR 355 ++W Y PPPP T + S+A + D++A Y P S R G + R Sbjct: 456 ASWHYVPPPPESTVEIVSNAPTLAMEDVQPTAASDTMALAPQEVYKP-SNENREGETITR 514 Query: 356 SG 357 SG Sbjct: 515 SG 516 >SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 436 Score = 29.5 bits (63), Expect = 3.2 Identities = 11/29 (37%), Positives = 17/29 (58%) Query: 298 WSYRPPPPVITEQPKSSATHFVPYERSYP 326 W Y P P++T S+A + VP++ YP Sbjct: 375 WGYAFPAPLLTPYEASAAFNVVPWKEVYP 403 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 29.5 bits (63), Expect = 3.2 Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 4/54 (7%) Query: 279 DSAALDLYYNPRPP-SDHSTWSYRPPPPVITEQPKSSATHFVPYERS---YPNT 328 DSA+ L N PP S H S+ PP P + P ++ T VP + YP+T Sbjct: 31 DSASPRLTPNLPPPFSVHQLQSFVPPQPPSSSSPSTTGTVAVPINGANAVYPST 84 >SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 29.1 bits (62), Expect = 4.2 Identities = 21/88 (23%), Positives = 33/88 (37%), Gaps = 2/88 (2%) Query: 264 GREERSRLQL--GALSPDSAALDLYYNPRPPSDHSTWSYRPPPPVITEQPKSSATHFVPY 321 G RS Q+ L P++ L++ + S W Y P I E K H V Sbjct: 31 GSSSRSTPQITGSPLDPNTPVKRLFFMRINIAQFSRWDYPYPREYIEELKKIHGVHHVEE 90 Query: 322 ERSYPNTLDSLAEKVHSYYPPESGSTRF 349 R P+ L++ + S +T + Sbjct: 91 NRDVPSILENYRNSKRKSHNFSSSNTPY 118 >SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar membrane transporter Hmt1|Schizosaccharomyces pombe|chr 3|||Manual Length = 830 Score = 28.7 bits (61), Expect = 5.6 Identities = 23/72 (31%), Positives = 34/72 (47%), Gaps = 6/72 (8%) Query: 101 FALQDTRHVVQLVEIVKRPGQTLGLYIREGDGGTRTDGVFISRIALESAVYNSGCLKVGD 160 FA + V+ + V +PG+ + L + E GG T I RI L NSG + + D Sbjct: 591 FAYDPRKPVLSDINFVAQPGKVIAL-VGESGGGKST----IMRILLRFFDVNSGSITIDD 645 Query: 161 -EILAVNLVDVR 171 +I V L +R Sbjct: 646 QDIRNVTLSSLR 657 >SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 28.7 bits (61), Expect = 5.6 Identities = 13/33 (39%), Positives = 22/33 (66%) Query: 161 EILAVNLVDVRRMSLDDVVIIMSIPRRLLLCTR 193 +ILA+++ D R L+D+ + ++ RLL CTR Sbjct: 161 KILAIDINDPRAKLLNDISDVQNLMPRLLPCTR 193 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.135 0.404 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 4,657,997 Number of Sequences: 5004 Number of extensions: 172220 Number of successful extensions: 429 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 410 Number of HSP's gapped (non-prelim): 16 length of query: 1292 length of database: 2,362,478 effective HSP length: 81 effective length of query: 1211 effective length of database: 1,957,154 effective search space: 2370113494 effective search space used: 2370113494 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 59 (27.9 bits)
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