SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000446-TA|BGIBMGA000446-PA|undefined
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC30C2.06c |dml1||mitochondrial genome maintenance protein |Sc...    45   2e-05
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    35   0.031
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    34   0.041
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    33   0.13 
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    31   0.29 
SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch...    30   0.67 
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    30   0.67 
SPBC13A2.04c |||PTR family peptide transporter|Schizosaccharomyc...    29   2.1  
SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub...    29   2.1  
SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|ch...    29   2.1  
SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei...    28   2.7  
SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces pom...    28   2.7  
SPBC2D10.18 |abc1|coq8|ABC1 kinase family protein|Schizosaccharo...    28   3.6  
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        28   3.6  
SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    27   4.7  
SPBC1685.08 |||histone deacetylase complex subunit Cti6|Schizosa...    27   4.7  
SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr...    27   4.7  
SPBC2D10.08c |||mitochondrial ribosomal protein subunit Yml6|Sch...    27   4.7  
SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|ch...    27   6.3  
SPAC630.05 |gyp7||GTPase activating protein Gyp7 |Schizosaccharo...    27   8.3  
SPBC3B8.09 |||U3 snoRNP-associated protein Utp3 |Schizosaccharom...    27   8.3  
SPBC29A10.02 |spo5|mrb1, mug12, SPBC365.18|meiotic RNA-binding p...    27   8.3  

>SPAC30C2.06c |dml1||mitochondrial genome maintenance protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 465

 Score = 45.2 bits (102), Expect = 2e-05
 Identities = 45/172 (26%), Positives = 70/172 (40%), Gaps = 8/172 (4%)

Query: 5   EILTLQFGHYSNYVGAHFWNIQELSFDYSGTATTDLNHDILYREGQTSRNEVTYT-PRLL 63
           EILT+ FG  SN+   HFWN QE  F Y       +N +  +R  +    E   T PR  
Sbjct: 3   EILTVTFGRKSNFCWTHFWNTQESYFVYDPNDHAKVNVNTNFRVLKKRDPEAIVTVPREC 62

Query: 64  IADLKGALKTLPQS--GGLSDDAQETDLQWD-TVEKIEEPPPEKNQYLAAIDSVQTTVAD 120
           I D         ++    L++ +      WD  + +I + P + + Y  A+ S +  + +
Sbjct: 63  IYDTPIEFGNTRKNWLEELNESSGGKGTAWDGKLTQIMQTPVDVHPYQEALWS-RDEIHE 121

Query: 121 GKKDYNLEENEAFDIYPTGRGVWKSDYGDLFSDNIRKYVEECDSMQGFQINF 172
           G    +  E E   I P     W SD+  LF D    +   C  +     +F
Sbjct: 122 GNAIES--EYELPSIRPKSVKYW-SDFNRLFLDTEYLFPVNCSELNSSDFSF 170



 Score = 43.6 bits (98), Expect = 7e-05
 Identities = 27/107 (25%), Positives = 52/107 (48%), Gaps = 6/107 (5%)

Query: 149 DLFSDNIRKYVEECDSMQGFQINFDCTDGFAGLALGCIEHLSDEYTKPILAYPIIASHFP 208
           +++ + +R  VEECDS+QGFQ   D    + G A   ++ + DE  +  +   +     P
Sbjct: 185 NVWDEGLRPLVEECDSVQGFQAAIDIDTPWGGFASEYMKVVQDELGECRVPTWVYGIREP 244

Query: 209 DNNSNTQEERDISNLRDSIRLVNILLSIQELSEHATMYVPLCTGEKG 255
             + ++      + + +    +N  LS+ +L+   + Y PLCT  +G
Sbjct: 245 IQSDSS------ATIDNHFGKLNEALSLSQLNGSCSQYFPLCTISQG 285


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 34.7 bits (76), Expect = 0.031
 Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 1/72 (1%)

Query: 129 ENEAFDIYPTGRGVWKSDYGDLFSDNIRKYVEECDSMQGFQINFDCTDGF-AGLALGCIE 187
           ++ A +I+  G     ++  D   D +R+  E CD++QGFQ+      G  +G+    + 
Sbjct: 94  QSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLS 153

Query: 188 HLSDEYTKPILA 199
            + +EY   ++A
Sbjct: 154 KIREEYPDRMMA 165



 Score = 32.7 bits (71), Expect = 0.13
 Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 11/82 (13%)

Query: 4  REILTLQFGHYSNYVGAHFWN--IQELSFDYSGT--ATTDLNHD---ILYREGQTSRNEV 56
          REI+ +Q G   N VGA FW+    E   D +G    T++  H+   + + E    +   
Sbjct: 2  REIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGK--- 58

Query: 57 TYTPRLLIADLKGALKTLPQSG 78
           Y PR ++ DL+       +SG
Sbjct: 59 -YVPRAVLVDLEPGTMDAVKSG 79


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 34.3 bits (75), Expect = 0.041
 Identities = 21/69 (30%), Positives = 32/69 (46%), Gaps = 3/69 (4%)

Query: 129 ENEAFDIYPTGRGVWKSDYGDLFSDNIRKYVEECDSMQGFQINFDCTDGFAGLALGC--I 186
           + +A + Y  G      +  D  +D IR+  + C  +QGF + F    G  G   G   +
Sbjct: 96  KEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLV-FHSFGGGTGSGFGALLL 154

Query: 187 EHLSDEYTK 195
           E L+ EYTK
Sbjct: 155 ERLAMEYTK 163



 Score = 27.9 bits (59), Expect = 3.6
 Identities = 12/39 (30%), Positives = 24/39 (61%)

Query: 474 DVSRIRFAKLHKFKEEGLEASEYTESLDKLAEFKDNYED 512
           D+   + A +H +  EG+E  E++E+ + LA  + +YE+
Sbjct: 396 DLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces
          pombe|chr 2|||Manual
          Length = 446

 Score = 32.7 bits (71), Expect = 0.13
 Identities = 24/74 (32%), Positives = 39/74 (52%), Gaps = 15/74 (20%)

Query: 4  REILTLQFGHYSNYVGAHFWNIQELSFDY----SGT----ATTDLNH-DILYREGQTSRN 54
          REI+TLQ G   N +G+ FW  Q+L  ++     GT    AT  ++  D+ + +   +R 
Sbjct: 3  REIITLQAGQCGNQIGSQFW--QQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTR- 59

Query: 55 EVTYTPRLLIADLK 68
             Y PR ++ DL+
Sbjct: 60 ---YIPRAILIDLE 70


>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1364

 Score = 31.5 bits (68), Expect = 0.29
 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 1/54 (1%)

Query: 170 INFDCTDGFA-GLALGCIEHLSDEYTKPILAYPIIASHFPDNNSNTQEERDISN 222
           IN      F+ GL L  +E      T+PI A P + +H P++ +N +EE D ++
Sbjct: 370 INLSPVSHFSNGLDLQNLEEAPMNLTRPINANPHLTNHSPNDLTNGEEEMDTTS 423


>SPAC688.11 |end4|sla2|Huntingtin-interacting protein
           homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1092

 Score = 30.3 bits (65), Expect = 0.67
 Identities = 28/126 (22%), Positives = 57/126 (45%), Gaps = 6/126 (4%)

Query: 378 KEAKAQQNLPAYRCNSVKEMFELYFQANNFLSATNLTVNEQPLNLKTPFPQIFTKNLNKY 437
           +E  AQQ         + E+ +        L  +N+ +N+    ++T   ++    +N  
Sbjct: 398 QEQLAQQQFQMQTQGQLAELEQQLLATRGQLEQSNVLLNQYDARVRTLENELSQAGVNLQ 457

Query: 438 GFI-KNDNRLEDVESCAVIAGYHNGTFLANMIEKLYRDVSRIRFAKLHKFKEEGLEASEY 496
             I +ND+ +E +++  ++   +    LA    KLY  + +     L K+K+  L+AS  
Sbjct: 458 EQIHQNDDLIESLKN-QILTWKNKYEALA----KLYTQLRQEHLDLLSKYKQIQLKASSA 512

Query: 497 TESLDK 502
            E++DK
Sbjct: 513 QEAIDK 518


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 30.3 bits (65), Expect = 0.67
 Identities = 20/69 (28%), Positives = 31/69 (44%), Gaps = 3/69 (4%)

Query: 129 ENEAFDIYPTGRGVWKSDYGDLFSDNIRKYVEECDSMQGFQINFDCTDGFAGLALGC--I 186
           + +A + Y  G      +  D   + IR+  + C  +QGF + F    G  G  LG   +
Sbjct: 100 KEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLV-FHSFGGGTGSGLGALLL 158

Query: 187 EHLSDEYTK 195
           E L+ EY K
Sbjct: 159 ERLNMEYGK 167



 Score = 27.9 bits (59), Expect = 3.6
 Identities = 12/39 (30%), Positives = 24/39 (61%)

Query: 474 DVSRIRFAKLHKFKEEGLEASEYTESLDKLAEFKDNYED 512
           D+   + A +H +  EG+E  E++E+ + LA  + +YE+
Sbjct: 400 DLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438


>SPBC13A2.04c |||PTR family peptide transporter|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 618

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 13/50 (26%), Positives = 24/50 (48%)

Query: 125 YNLEENEAFDIYPTGRGVWKSDYGDLFSDNIRKYVEECDSMQGFQINFDC 174
           +NLE+ +       GR   K  + DLF D +++ +  C +   + I + C
Sbjct: 323 FNLEKTKPSCTTTVGRVTLKDQWDDLFIDELKRALRACKTFLFYPIYWVC 372


>SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory
           subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 838

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 1/75 (1%)

Query: 91  WDTVEKIEEPPPEKNQ-YLAAIDSVQTTVADGKKDYNLEENEAFDIYPTGRGVWKSDYGD 149
           +D V+++   P +K+Q Y  A+D  +      K  Y  E N+     P+G       +  
Sbjct: 547 YDVVQQVLNAPMDKDQNYALAVDMFKQGKITEKIVYGQELNDKKVAKPSGFRAGYMGHLT 606

Query: 150 LFSDNIRKYVEECDS 164
           + +D + K+VE   S
Sbjct: 607 IIADEVVKFVEHYSS 621


>SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 299

 Score = 28.7 bits (61), Expect = 2.1
 Identities = 22/101 (21%), Positives = 42/101 (41%), Gaps = 5/101 (4%)

Query: 178 FAGLALGCIEHLSDEYTKPILAYPIIASHFPDNNSNTQEERDISNLRDSIRLVNILLSIQ 237
           F    L  ++++S+       ++P+     PD +S   + +D+  L++ I+  NI   + 
Sbjct: 190 FQSAMLPMLDNISNHRFGAPFSHPVNRKEAPDYDSLVYKPQDLRTLKNMIKEGNI-TEVD 248

Query: 238 ELSEHATMYVPLCTGEKGWRKPGNARTF---DCLNYDPKLY 275
           EL          C    G   P NA +    +C  Y  +L+
Sbjct: 249 ELYREVLRIFANCKMYNG-SDPANAMSIWGDECFRYTEELF 288


>SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated
            protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1462

 Score = 28.3 bits (60), Expect = 2.7
 Identities = 10/39 (25%), Positives = 22/39 (56%)

Query: 464  LANMIEKLYRDVSRIRFAKLHKFKEEGLEASEYTESLDK 502
            ++N +   ++D +R  F +++  +E G E + Y  +L K
Sbjct: 986  ISNSVSSSFKDFTRTPFKEINGERETGFELTSYVNALSK 1024


>SPCC1919.11 |mug137||BAR adaptor protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 420

 Score = 28.3 bits (60), Expect = 2.7
 Identities = 10/28 (35%), Positives = 18/28 (64%)

Query: 405 NNFLSATNLTVNEQPLNLKTPFPQIFTK 432
           N+ LS+ N+  + QP+ ++ P P+I  K
Sbjct: 393 NSLLSSQNVEASSQPIKIRKPLPEIPNK 420


>SPBC2D10.18 |abc1|coq8|ABC1 kinase family
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 610

 Score = 27.9 bits (59), Expect = 3.6
 Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 4/46 (8%)

Query: 354 IANDKLFQLITI----RGIPESYLKAPPKEAKAQQNLPAYRCNSVK 395
           +AN  L++LITI    R + +SYLKA      A+Q   A +  S K
Sbjct: 1   MANSGLWELITIGSAVRNVAKSYLKAEASSITAKQLYDASKITSSK 46


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 27.9 bits (59), Expect = 3.6
 Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 3/53 (5%)

Query: 80   LSDDAQ-ETDLQWDTVEKIEEPPPEKNQYLAAIDSVQTTVADG--KKDYNLEE 129
            +SDD   + ++Q D  E   +PPPE   +L   + ++    +G   KD +LE+
Sbjct: 4055 MSDDVGIDDEIQPDIQENNSQPPPENEDHLDLPEDLKLDEKEGDVSKDSDLED 4107


>SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 674

 Score = 27.5 bits (58), Expect = 4.7
 Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 2/43 (4%)

Query: 209 DNNSNTQEERDISNLRDSIRLVNILLSI--QELSEHATMYVPL 249
           D NS   E RD   L D++ + N+L     QEL  H   + PL
Sbjct: 261 DKNSINHEIRDNEGLEDTVFVRNLLFECTEQELYNHFRQFGPL 303


>SPBC1685.08 |||histone deacetylase complex subunit
           Cti6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 424

 Score = 27.5 bits (58), Expect = 4.7
 Identities = 19/86 (22%), Positives = 39/86 (45%), Gaps = 2/86 (2%)

Query: 191 DEYTKPILAYPIIASHFPDNNSNTQEERDISNLRDSIRLVNILLSIQELSEHATMYVPLC 250
           +E ++ I   P+   H P+ + +  EE + S+  +  R V  ++   + +    +Y+  C
Sbjct: 13  EEESEKIQKSPVDTPHTPNGSVSDNEENETSSTGEVTRCVCGIVESDDEASDGGLYIQ-C 71

Query: 251 TGEKGWRKPGNARTFDCLNYDPKLYY 276
                W+  GN   F   +  P++YY
Sbjct: 72  DQCSVWQH-GNCVGFADESEVPEVYY 96


>SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 819

 Score = 27.5 bits (58), Expect = 4.7
 Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 1/51 (1%)

Query: 73  TLPQSGGLSDDAQETDLQWDTVEKIEEPPPEKNQYLAAIDSVQTTVADGKK 123
           +L ++    DD+   D  W T+E    PP ++   L A+DS   T+   K+
Sbjct: 133 SLSETSNALDDSCFND-DWITIESEFSPPIDRGMTLEAMDSQNDTLFTSKQ 182


>SPBC2D10.08c |||mitochondrial ribosomal protein subunit
           Yml6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 261

 Score = 27.5 bits (58), Expect = 4.7
 Identities = 26/88 (29%), Positives = 40/88 (45%), Gaps = 14/88 (15%)

Query: 375 APPKEAKAQQNLPAYRCNSVKEMFELYFQANNFLSATN-----LTVNEQPLNLKTPFPQI 429
           +P +   A+ + P  R  S K   ++Y  A     +T      LT+ E P+NL  P  +I
Sbjct: 85  SPIRRGGAKSHGPKPRDFSTKLQTQVYNLAMRIALSTRFINNELTILENPINLSQPKTRI 144

Query: 430 FTKNL------NKYG---FIKNDNRLED 448
             + L      ++YG   F+ NDN  ED
Sbjct: 145 LLEVLKQHKLGHEYGKALFVLNDNYFED 172


>SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 862

 Score = 27.1 bits (57), Expect = 6.3
 Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 10/72 (13%)

Query: 165 MQGFQINFDCTDGFAGLALGCIEHLSDEYTKPILAYPIIASHFPDNNSNTQEERDISNLR 224
           M+G  +    + G A LA+  I  +++     ++AY  +  HFP  + NT     ++   
Sbjct: 470 MRGITVGVIGSSGSAQLAIFFILEVAN-----VVAYAYVRPHFPQTSMNT-----LNTFI 519

Query: 225 DSIRLVNILLSI 236
            ++RL+ ++L I
Sbjct: 520 STMRLITVILMI 531


>SPAC630.05 |gyp7||GTPase activating protein Gyp7
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 743

 Score = 26.6 bits (56), Expect = 8.3
 Identities = 11/38 (28%), Positives = 20/38 (52%)

Query: 363 ITIRGIPESYLKAPPKEAKAQQNLPAYRCNSVKEMFEL 400
           I +  +P+S+LK  P++    QN       S++E  E+
Sbjct: 72  ILLSWVPDSFLKNRPRDLSVFQNAETLSNGSIREWVEI 109


>SPBC3B8.09 |||U3 snoRNP-associated protein Utp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 597

 Score = 26.6 bits (56), Expect = 8.3
 Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 3/39 (7%)

Query: 432 KNLNKYGFIKNDNRLEDVESCAVIAGYHNGTFLANMIEK 470
           KNL+ YG     NRL+DV++   IA   +  F AN I++
Sbjct: 374 KNLDDYG---EGNRLDDVDAEDKIARKRSLKFYANQIDQ 409


>SPBC29A10.02 |spo5|mrb1, mug12, SPBC365.18|meiotic RNA-binding
           protein 1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 567

 Score = 26.6 bits (56), Expect = 8.3
 Identities = 12/36 (33%), Positives = 19/36 (52%)

Query: 386 LPAYRCNSVKEMFELYFQANNFLSATNLTVNEQPLN 421
           L  Y+C+  KE F    Q+     +TNL ++  PL+
Sbjct: 359 LCGYQCSFAKESFSARLQSLQDTESTNLYISNLPLH 394


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.317    0.134    0.397 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,575,650
Number of Sequences: 5004
Number of extensions: 115985
Number of successful extensions: 374
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 355
Number of HSP's gapped (non-prelim): 30
length of query: 516
length of database: 2,362,478
effective HSP length: 76
effective length of query: 440
effective length of database: 1,982,174
effective search space: 872156560
effective search space used: 872156560
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 56 (26.6 bits)

- SilkBase 1999-2023 -