BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000425-TA|BGIBMGA000425- PA|IPR002123|Phospholipid/glycerol acyltransferase (310 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC428.14 |||1-acylglycerol-3-phosphate acyltransferase |Schizo... 116 6e-27 SPAC1851.02 |||1-acylglycerol-3-phosphate O-acyltransferase|Schi... 44 4e-05 SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 28 1.5 SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 28 1.5 SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosa... 27 3.4 SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schi... 27 4.5 SPAC2F7.09c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 4.5 SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr... 27 4.5 >SPBC428.14 |||1-acylglycerol-3-phosphate acyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 350 Score = 116 bits (278), Expect = 6e-27 Identities = 68/241 (28%), Positives = 115/241 (47%), Gaps = 7/241 (2%) Query: 17 EHGYLIMNHSYEIDWLMGWQFCDGIGVLGNCKAYAKKSIQYLPPIGWMWKFSEFVFLERS 76 E +I NH DW+ W G+ K S+++LP IGW + F+FL R Sbjct: 94 ERNIVIANHQLYSDWMYVWWLSYTAKQHGHVYIMLKNSLKWLPVIGWGMQLFRFIFLSRK 153 Query: 77 FEKDKEIIKKQISELCDYPDPVWLLLTPEGTRYTKTKHEASVNFAKEKNLPILKHHLTPR 136 ++KD E + + + + D V L+L PEGT ++ ++ S +A + + + KH + PR Sbjct: 154 WDKDYETMSRHFKFIRNVRDSVSLILFPEGTNLVESTYQRSRVYADKIGVKMPKHLMLPR 213 Query: 137 TRGFTTSLQFFRGKIPAIYNIQLAF-----EKESKTPPTLTSLLY-GKPV-HAHMYIERI 189 RG S+ R + +Y+ F +K + +L L + G P+ H+++ R Sbjct: 214 VRGLFYSISQLRDSMTYLYDYTFYFSDPSPKKYAADAFSLPKLFFEGVPIKRLHIHVRRF 273 Query: 190 PVENIPEDEAEATKWMHDLFVTKDKMQDSFLNTGDFFLQSGVERVEAFTSRPRIYSLLNT 249 P+ IP +E + T W++ + KDK+ D+ L TG+F + R I SL + Sbjct: 274 PISEIPTEEDQFTDWLYQRWYEKDKLIDTLLETGNFPGPKKLHTTVRLKHRLEILSLFSV 333 Query: 250 L 250 L Sbjct: 334 L 334 >SPAC1851.02 |||1-acylglycerol-3-phosphate O-acyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 279 Score = 43.6 bits (98), Expect = 4e-05 Identities = 46/179 (25%), Positives = 77/179 (43%), Gaps = 18/179 (10%) Query: 7 KDEYEKFYGK-EHGYLIMNHSYEIDWL-MGWQFCDGIGVLGNCKAYAKKSIQYLPPIGWM 64 K E E+ K + L++NH E+D L +G F V+ AKKS++Y+P +GW Sbjct: 88 KIENEEILRKHKSAVLVVNHQSELDILAIGRTFGPNYSVI------AKKSLRYVPILGWF 141 Query: 65 WKFSEFVFLERSFEKDK-EIIKKQISELCDYPDPVWLLLTPEGTRYTKTK------HEAS 117 S+ VF++RS D ++ K + +W+ EGTR K + + Sbjct: 142 MILSDVVFIDRSRRSDAIQLFAKAARRMRKENISIWVF--AEGTRSYSLKPCLLPLKKGA 199 Query: 118 VNFAKEKNLPILKHHLTPRTRGFTTSLQFFRGKIPAIYNIQLAFEKESKTPPTLTSLLY 176 + A + +PI+ + F + F K A+ + E KT + LL+ Sbjct: 200 FHLAVQAQVPIIPIAIQTYGHLFHPPTKVF-NKGEALIKVLDPIPTEGKTAEDVNDLLH 257 >SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 28.3 bits (60), Expect = 1.5 Identities = 16/35 (45%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Query: 111 KTKHEASVNFAKEKNLPILKHHLTPRTRGFTTSLQ 145 +TKH +SVN KE+N IL+ TP F LQ Sbjct: 232 ETKHFSSVNVCKEENFAILE-VATPEDATFLWGLQ 265 >SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 28.3 bits (60), Expect = 1.5 Identities = 17/69 (24%), Positives = 30/69 (43%) Query: 107 TRYTKTKHEASVNFAKEKNLPILKHHLTPRTRGFTTSLQFFRGKIPAIYNIQLAFEKESK 166 T YT+ +A ++ +L L L+P TRG + F K+ + +A ++ K Sbjct: 556 TLYTRLIEDALGEYSYPASLAGLSFSLSPSTRGIILCISGFTDKLHVLLEKVVAMMRDLK 615 Query: 167 TPPTLTSLL 175 P +L Sbjct: 616 VHPQRFEIL 624 >SPBC776.12c |hsk1||serine/threonine protein kinase Hsk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 27.1 bits (57), Expect = 3.4 Identities = 10/25 (40%), Positives = 17/25 (68%) Query: 72 FLERSFEKDKEIIKKQISELCDYPD 96 F E SFEKD+++ K +S + D+ + Sbjct: 453 FGETSFEKDEDLTAKHLSHILDFKE 477 >SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 26.6 bits (56), Expect = 4.5 Identities = 14/44 (31%), Positives = 21/44 (47%) Query: 122 KEKNLPILKHHLTPRTRGFTTSLQFFRGKIPAIYNIQLAFEKES 165 K+ NLPI T R G S FR K+ + + Q+ +E + Sbjct: 54 KQNNLPIYIGRYTERYNGGDVSAIVFRSKVVSRRHAQIFYENNT 97 >SPAC2F7.09c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 491 Score = 26.6 bits (56), Expect = 4.5 Identities = 11/34 (32%), Positives = 19/34 (55%) Query: 112 TKHEASVNFAKEKNLPILKHHLTPRTRGFTTSLQ 145 TK ++ N K N+P++ H +P FT S++ Sbjct: 399 TKLQSLENSTKNDNVPLVTHSPSPMHSSFTVSIK 432 >SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 508 Score = 26.6 bits (56), Expect = 4.5 Identities = 12/30 (40%), Positives = 16/30 (53%), Gaps = 1/30 (3%) Query: 35 WQFCDGIG-VLGNCKAYAKKSIQYLPPIGW 63 W +G+G +LG+C AY LP GW Sbjct: 193 WVAFNGLGQILGSCMAYGLAKRTSLPMRGW 222 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.322 0.138 0.429 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,483,366 Number of Sequences: 5004 Number of extensions: 64088 Number of successful extensions: 140 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 131 Number of HSP's gapped (non-prelim): 8 length of query: 310 length of database: 2,362,478 effective HSP length: 73 effective length of query: 237 effective length of database: 1,997,186 effective search space: 473333082 effective search space used: 473333082 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 54 (25.8 bits)
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