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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000422-TA|BGIBMGA000422-PA|IPR002076|GNS1/SUR4 membrane
protein
         (343 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_7377| Best HMM Match : ELO (HMM E-Value=3.6e-06)                   122   6e-28
SB_7376| Best HMM Match : ELO (HMM E-Value=3.3e-07)                    96   4e-20
SB_3707| Best HMM Match : No HMM Matches (HMM E-Value=.)               42   0.001
SB_3799| Best HMM Match : No HMM Matches (HMM E-Value=.)               40   0.003
SB_4954| Best HMM Match : No HMM Matches (HMM E-Value=.)               36   0.063
SB_14072| Best HMM Match : ELO (HMM E-Value=5.7)                       32   0.59 
SB_8858| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   1.4  
SB_46680| Best HMM Match : Helicase_C (HMM E-Value=3.8e-22)            30   2.4  
SB_786| Best HMM Match : EGF (HMM E-Value=0)                           30   2.4  
SB_4955| Best HMM Match : rve (HMM E-Value=7.5e-09)                    30   2.4  
SB_43724| Best HMM Match : AMP-binding (HMM E-Value=2.6e-09)           29   7.3  
SB_2463| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   9.6  

>SB_7377| Best HMM Match : ELO (HMM E-Value=3.6e-06)
          Length = 255

 Score =  122 bits (293), Expect = 6e-28
 Identities = 65/169 (38%), Positives = 94/169 (55%), Gaps = 6/169 (3%)

Query: 102 LFFVLRKKNEHVSTLHVIHHGIMPMSVWFGLKFAPGGHSTFFALLNTFVHIVMYFYYMVA 161
           +FF+LRKKN  +S LHV HH  M    W G+K+  GG S F A++N F+HI+MY YY ++
Sbjct: 86  MFFILRKKNNQISFLHVYHHFSMVCLWWVGIKWVAGGQSFFSAMINAFIHIIMYAYYGLS 145

Query: 162 AMGPKYQKYIWWKKYLTAFQMVQFVLIFSHQLQVLFRPSCQYPRVFVYWIAMHGFLFLFL 221
           A+ P+ + Y+WWKKYLT  Q+ QF  + +H +  L+   C++     Y +  +   FL L
Sbjct: 146 AI-PEMRIYLWWKKYLTQLQLTQFCTVMAHGVLSLW-AKCRFFLWMQYALIGYMISFLIL 203

Query: 222 FSDFYKARYTTAERKSKAQNGLCMAVMDDSSSSLNGRNGYKQEMGSEVP 270
           FS+FY   Y T  RK    N    A+  +  +      G KQ  G + P
Sbjct: 204 FSNFYLHAYLT--RKQSCPNK--DAMQANGETPFCPDKGAKQANGVDTP 248



 Score = 37.1 bits (82), Expect = 0.021
 Identities = 18/43 (41%), Positives = 27/43 (62%), Gaps = 1/43 (2%)

Query: 14 YAYCAKEL-GPKLMANRKPFELRNILVVYNMAQTIFSAWIFYE 55
          +AY    L GP LM N+K F L+ I+V+YN   ++ + +IF E
Sbjct: 43 FAYILAVLTGPHLMKNKKAFNLQWIMVIYNGMMSLLNLYIFLE 85


>SB_7376| Best HMM Match : ELO (HMM E-Value=3.3e-07)
          Length = 278

 Score = 95.9 bits (228), Expect = 4e-20
 Identities = 63/209 (30%), Positives = 100/209 (47%), Gaps = 36/209 (17%)

Query: 36  NILVVYNMAQ----TIFSAWIFYEYLMSGWW-GHYDFRCQLVDYSRSPMAMRMANTCWWY 90
           N ++ Y++ Q    T+F    ++E++ S      ++  CQ V Y   P  MR+A  C+ Y
Sbjct: 100 NAILFYSIWQIHYLTLFVVDGYFEFIASILSIPEFNLMCQDVSYEEDPRLMRLARVCYIY 159

Query: 91  YFSKFTEFMDTLFFVLRKKNEHVSTLHVIHHGIMPMSVWFGLKFAPGGHSTFFALLNTFV 150
           Y SKF               E+  T  V+    +  + +FG            A  N+F+
Sbjct: 160 YLSKFV--------------EYFDTYVVV---AVVFTAYFG------------AACNSFI 190

Query: 151 HIVMYFYYMVAAMGPKYQKYIWWKKYLTAFQMVQFVLIFSHQLQVLFRPSCQYPRVFVYW 210
           H++MY YY ++AMGP  QKY+WWK+YLT  Q++QF+ +    +  L+   C   + F  W
Sbjct: 191 HVIMYLYYGLSAMGPSVQKYLWWKRYLTKMQLIQFLALIYMAVYALWHDGCGTHK-FFQW 249

Query: 211 IAM-HGFLFLFLFSDFYKARYTTAERKSK 238
           +   +G   L LFS+FY   Y     K +
Sbjct: 250 VQFGYGMSLLVLFSNFYLHTYLKKPSKKE 278



 Score = 58.4 bits (135), Expect = 8e-09
 Identities = 25/55 (45%), Positives = 37/55 (67%), Gaps = 1/55 (1%)

Query: 1  MSSPWPTLAACVCYAYCAKELGPKLMANRKPFELRNILVVYNMAQTIFSAWIFYE 55
          +++PWP L+    Y +  K +GPK M NRK ++LR +LVVYN A  + SA++ YE
Sbjct: 28 IATPWPCLSLMAMYLFIVK-VGPKFMENRKAWDLRGVLVVYNFALVLLSAYMVYE 81


>SB_3707| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 89

 Score = 41.5 bits (93), Expect = 0.001
 Identities = 22/62 (35%), Positives = 31/62 (50%), Gaps = 1/62 (1%)

Query: 283 FDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAK-RVPHISFDVNEPKRVTQIISFDVN 341
           FD  K + DT+ + F   K K  T+ + FD  KAK     + FD  + K  TQ + FD  
Sbjct: 3   FDTKKVKFDTKKVKFDTEKVKFDTEKVKFDTEKAKFDTEKVKFDTQKVKFDTQKVKFDTQ 62

Query: 342 KL 343
           K+
Sbjct: 63  KV 64



 Score = 40.7 bits (91), Expect = 0.002
 Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 1/63 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAK-RVPHISFDVNEPKRVTQIISFD 339
           + FD  K + DT+ + F   K K  T+ + FD  K K     + FD  + K  TQ + FD
Sbjct: 15  VKFDTEKVKFDTEKVKFDTEKAKFDTEKVKFDTQKVKFDTQKVKFDTQKVKFDTQKVKFD 74

Query: 340 VNK 342
             K
Sbjct: 75  TQK 77



 Score = 39.1 bits (87), Expect = 0.005
 Identities = 21/64 (32%), Positives = 30/64 (46%), Gaps = 1/64 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAK-RVPHISFDVNEPKRVTQIISFD 339
           + FD  K + DT+ + F   K K  T+   FD  K K     + FD  + K  TQ + FD
Sbjct: 8   VKFDTKKVKFDTEKVKFDTEKVKFDTEKAKFDTEKVKFDTQKVKFDTQKVKFDTQKVKFD 67

Query: 340 VNKL 343
             K+
Sbjct: 68  TQKV 71



 Score = 38.7 bits (86), Expect = 0.007
 Identities = 22/65 (33%), Positives = 29/65 (44%), Gaps = 1/65 (1%)

Query: 279 DALSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAK-RVPHISFDVNEPKRVTQIIS 337
           + + FD  K + DT+   F   K K  TQ + FD  K K     + FD  + K  TQ   
Sbjct: 20  EKVKFDTEKVKFDTEKAKFDTEKVKFDTQKVKFDTQKVKFDTQKVKFDTQKVKFDTQKAK 79

Query: 338 FDVNK 342
           FD  K
Sbjct: 80  FDTQK 84



 Score = 38.7 bits (86), Expect = 0.007
 Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 1/62 (1%)

Query: 279 DALSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAK-RVPHISFDVNEPKRVTQIIS 337
           + + FD  K + DT+ + F   K K  TQ + FD  K K     + FD  + K  TQ   
Sbjct: 27  EKVKFDTEKAKFDTEKVKFDTQKVKFDTQKVKFDTQKVKFDTQKVKFDTQKAKFDTQKAK 86

Query: 338 FD 339
           FD
Sbjct: 87  FD 88



 Score = 35.1 bits (77), Expect = 0.084
 Identities = 17/44 (38%), Positives = 22/44 (50%)

Query: 274 ASSGADALSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAK 317
           A    + + FD  K + DTQ + F   K K  TQ + FD  KAK
Sbjct: 36  AKFDTEKVKFDTQKVKFDTQKVKFDTQKVKFDTQKVKFDTQKAK 79


>SB_3799| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 291

 Score = 39.9 bits (89), Expect = 0.003
 Identities = 30/88 (34%), Positives = 46/88 (52%), Gaps = 12/88 (13%)

Query: 34  LRNILVVYNMAQTIFSAW-IFYE--YLMSGWWGHYDFRCQLVDYSRSPMAMRMANTCWWY 90
           LR +++ YN+   I SA+ + Y    L+ GW  H  F   L   +R+  A+ +      Y
Sbjct: 58  LRKLMIAYNLLCAILSAYSVLYAGVALVEGW-PHSLFDLTLD--TRAQHALHV------Y 108

Query: 91  YFSKFTEFMDTLFFVLRKKNEHVSTLHV 118
           + +K  E MDT+F +LR K   +S LHV
Sbjct: 109 WLTKKIELMDTVFMILRPKTRQMSFLHV 136


>SB_4954| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 165

 Score = 35.5 bits (78), Expect = 0.063
 Identities = 20/61 (32%), Positives = 38/61 (62%), Gaps = 1/61 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAKRV-PHISFDVNEPKRVTQIISFD 339
           +S+DV   +    ++S+ V +PK +  ++S+DV + K +   IS+DV  PK +  ++S+D
Sbjct: 94  MSYDVPGPKIIMVMMSYDVPRPKIIMVMMSYDVPRPKIILVMISYDVPCPKIIMVMMSYD 153

Query: 340 V 340
           V
Sbjct: 154 V 154



 Score = 31.5 bits (68), Expect = 1.0
 Identities = 18/58 (31%), Positives = 35/58 (60%), Gaps = 1/58 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAKRV-PHISFDVNEPKRVTQIIS 337
           +S+DV + +    ++S+ V +PK +  +IS+DV   K +   +S+DV  PK +  ++S
Sbjct: 108 MSYDVPRPKIIMVMMSYDVPRPKIILVMISYDVPCPKIIMVMMSYDVPRPKIIMVMMS 165


>SB_14072| Best HMM Match : ELO (HMM E-Value=5.7)
          Length = 226

 Score = 32.3 bits (70), Expect = 0.59
 Identities = 21/83 (25%), Positives = 41/83 (49%), Gaps = 6/83 (7%)

Query: 37  ILVVYNMAQTIFSAWIFYEYLMSGWWGHYDFRCQLVDYSRSPMAMRMANTCWWYYFSKFT 96
           +LV+YN+  ++ S    Y +++     HY  +  +    R  +   +      Y+ +K  
Sbjct: 108 VLVLYNLLCSVLS---LYSFVIIA--KHY-VQNGVESLFRMEVNQSIKQALLVYWATKNI 161

Query: 97  EFMDTLFFVLRKKNEHVSTLHVI 119
           E +DT+F +LR +   +S LH+I
Sbjct: 162 ELLDTVFMILRHRQRQISFLHLI 184


>SB_8858| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 886

 Score = 31.1 bits (67), Expect = 1.4
 Identities = 20/50 (40%), Positives = 25/50 (50%), Gaps = 1/50 (2%)

Query: 290 RDTQIISFTVNKPKRVTQIISFD-VNKAKRVPHISFDVNEPKRVTQIISF 338
           R TQ++ FT   P R TQ+I F   N  +    I F    P R TQ+I F
Sbjct: 185 RYTQLMVFTRYNPVRYTQLIVFTRYNPVRYTQLIVFTRYNPVRYTQLIVF 234



 Score = 29.1 bits (62), Expect = 5.5
 Identities = 19/50 (38%), Positives = 24/50 (48%), Gaps = 1/50 (2%)

Query: 290 RDTQIISFTVNKPKRVTQIISFD-VNKAKRVPHISFDVNEPKRVTQIISF 338
           R TQ+I FT   P R TQ+I F   N  +    I F    P R  Q++ F
Sbjct: 199 RYTQLIVFTRYNPVRYTQLIVFTRYNPVRYTQLIVFTRYNPVRYAQLMVF 248


>SB_46680| Best HMM Match : Helicase_C (HMM E-Value=3.8e-22)
          Length = 1058

 Score = 30.3 bits (65), Expect = 2.4
 Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 7/71 (9%)

Query: 250  DSSSSLNGRNGYKQEMGSEVPSSYASSGADALSFDVNKTERDTQIISFTVNKPKRVTQII 309
            D+SS LN +   + E+G E     +S+  DALS       +  Q + +    PKRVT ++
Sbjct: 939  DTSSVLNAKPRSRPELGGENLVKISSTSLDALS-------KSRQKVMYAHEPPKRVTPVV 991

Query: 310  SFDVNKAKRVP 320
                +K    P
Sbjct: 992  EGRSSKGDDSP 1002


>SB_786| Best HMM Match : EGF (HMM E-Value=0)
          Length = 1427

 Score = 30.3 bits (65), Expect = 2.4
 Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 2/72 (2%)

Query: 45  QTIFSAWIFYEYLMSGWWGHYDFRCQLVDYSRSPMAMRMANTCWWYYFSKFTEFMDTLFF 104
           QTIFS  +    L  G W          DYSR+   +  A T   +YFS    F+D +  
Sbjct: 639 QTIFSV-LTPPVLTEGKWTQVTVTYSS-DYSRARTIVTKATTLLLFYFSSSLIFVDDIII 696

Query: 105 VLRKKNEHVSTL 116
           +  K N+ +  L
Sbjct: 697 ISVKDNDLLKLL 708


>SB_4955| Best HMM Match : rve (HMM E-Value=7.5e-09)
          Length = 667

 Score = 30.3 bits (65), Expect = 2.4
 Identities = 17/61 (27%), Positives = 34/61 (55%), Gaps = 1/61 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAKRV-PHISFDVNEPKRVTQIISFD 339
           +S+DV   +     +SF +  PK +   +S+DV + K +   +S+DV  P  +  ++S+D
Sbjct: 4   MSYDVPGPKIIMVTMSFDIPGPKIIMVTMSYDVPRPKIIMVMMSYDVPGPNIIMVMMSYD 63

Query: 340 V 340
           +
Sbjct: 64  I 64



 Score = 29.5 bits (63), Expect = 4.2
 Identities = 17/61 (27%), Positives = 34/61 (55%), Gaps = 1/61 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAKRV-PHISFDVNEPKRVTQIISFD 339
           +SFD+   +     +S+ V +PK +  ++S+DV     +   +S+D+  PK +   +S+D
Sbjct: 18  MSFDIPGPKIIMVTMSYDVPRPKIIMVMMSYDVPGPNIIMVMMSYDIPCPKIIMVTMSYD 77

Query: 340 V 340
           V
Sbjct: 78  V 78



 Score = 28.7 bits (61), Expect = 7.3
 Identities = 15/61 (24%), Positives = 35/61 (57%), Gaps = 1/61 (1%)

Query: 281 LSFDVNKTERDTQIISFTVNKPKRVTQIISFDVNKAKRV-PHISFDVNEPKRVTQIISFD 339
           +S+DV + +    ++ + V  PK +  ++S+D+   K +   +S+D+  PK +  ++ +D
Sbjct: 74  MSYDVPRPKIIMVMMIYNVPGPKIIMVMMSYDIPCPKIIMVMMSYDIPGPKIIMVMMGYD 133

Query: 340 V 340
           V
Sbjct: 134 V 134


>SB_43724| Best HMM Match : AMP-binding (HMM E-Value=2.6e-09)
          Length = 490

 Score = 28.7 bits (61), Expect = 7.3
 Identities = 11/48 (22%), Positives = 28/48 (58%)

Query: 149 FVHIVMYFYYMVAAMGPKYQKYIWWKKYLTAFQMVQFVLIFSHQLQVL 196
           F+ +++Y  Y+    G +Y++ + +K+YL   +  +++  F H + +L
Sbjct: 425 FIEVLLYNIYLHGPEGVRYKEALIYKRYLPCPEGARYLDDFIHVVMLL 472


>SB_2463| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 941

 Score = 28.3 bits (60), Expect = 9.6
 Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 3/43 (6%)

Query: 133 KFAPGGHSTFFALLNTF---VHIVMYFYYMVAAMGPKYQKYIW 172
           K+   G STFF L+ +F    H +   +Y++A    KY  + W
Sbjct: 195 KYLEYGASTFFLLMLSFCLVAHWMACMFYVIATKYDKYDVHCW 237


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.328    0.137    0.448 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,063,962
Number of Sequences: 59808
Number of extensions: 438642
Number of successful extensions: 1230
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1184
Number of HSP's gapped (non-prelim): 31
length of query: 343
length of database: 16,821,457
effective HSP length: 83
effective length of query: 260
effective length of database: 11,857,393
effective search space: 3082922180
effective search space used: 3082922180
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 60 (28.3 bits)

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