GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000422-TA|BGIBMGA000422-PA|IPR002076|GNS1/SUR4 membrane
protein
         (343 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g06460.1 68416.m00748 GNS1/SUR4 membrane family protein simil...    72   5e-13
At3g06470.1 68416.m00749 GNS1/SUR4 membrane family protein simil...    67   2e-11
At4g36830.1 68417.m05223 GNS1/SUR4 membrane family protein weak ...    30   2.0  
At3g27520.1 68416.m03440 expressed protein                             29   6.0  
At4g31730.1 68417.m04504 expressed protein                             28   7.9  
At3g48050.2 68416.m05239 bromo-adjacent homology (BAH) domain-co...    28   7.9  
At3g48050.1 68416.m05238 bromo-adjacent homology (BAH) domain-co...    28   7.9  

>At3g06460.1 68416.m00748 GNS1/SUR4 membrane family protein similar
           to SP|P25358 Elongation of fatty acids protein 2 (GNS1
           protein) (V-SNARE bypass mutant gene 2 protein)
           {Saccharomyces cerevisiae}; contains Pfam profile
           PF01151: GNS1/SUR4 family
          Length = 298

 Score = 72.1 bits (169), Expect = 5e-13
 Identities = 37/96 (38%), Positives = 55/96 (57%), Gaps = 5/96 (5%)

Query: 90  YYFSKFTEFMDTLFFVLRKKNEHVSTLHVIHHGIMPMSVWFGLKFAPGGHSTFFALLNTF 149
           +Y SK  EF+DTL  +L K  + +S LHV HH  + +  +  L+           +LN+ 
Sbjct: 124 FYLSKILEFVDTLLIILNKSIQRLSFLHVYHHATVVILCYLWLRTRQSMFPVGL-VLNST 182

Query: 150 VHIVMYFYYMVAAMGPKYQKYIWWKKYLTAFQMVQF 185
           VH++MY YY + A+G + +    WKK +T FQMVQF
Sbjct: 183 VHVIMYGYYFLCAIGSRPK----WKKLVTNFQMVQF 214


>At3g06470.1 68416.m00749 GNS1/SUR4 membrane family protein similar
           to SP|P39540 Elongation of fatty acids protein 1
           {Saccharomyces cerevisiae}; contains Pfam profile
           PF01151: GNS1/SUR4 family
          Length = 278

 Score = 66.9 bits (156), Expect = 2e-11
 Identities = 49/147 (33%), Positives = 75/147 (51%), Gaps = 14/147 (9%)

Query: 90  YYFSKFTEFMDTLFFVLRKKNEHVSTLHVIHHGIMPMSVWFGLKFAPGGHSTF-FALL-N 147
           +Y SK  EF DT+  +L K  + +S LHV HH  + +  +  L+      S F  AL+ N
Sbjct: 127 FYLSKILEFGDTILIILGKSIQRLSFLHVYHHATVVVMCYLWLRTR---QSMFPIALVTN 183

Query: 148 TFVHIVMYFYYMVAAMGPKYQKYIWWKKYLTAFQMVQFVLIFSHQ----LQVLFRPSCQY 203
           + VH++MY YY + A+G + +    WK+ +T  Q+VQFV  F        + LF   C  
Sbjct: 184 STVHVIMYGYYFLCAVGSRPK----WKRLVTDCQIVQFVFSFGLSGWMLREHLFGSGCTG 239

Query: 204 PRVFVYWIAMHGFLFLFLFSDFYKARY 230
              + +  A +  L L LFS+F+   Y
Sbjct: 240 IWGWCFNAAFNASL-LALFSNFHSKNY 265


>At4g36830.1 68417.m05223 GNS1/SUR4 membrane family protein weak
           similarity to long chain polyunsaturated fatty acid
           elongation enzyme [Isochrysis galbana] GI:17226123;
           contains Pfam profile PF01151: GNS1/SUR4 family
          Length = 289

 Score = 30.3 bits (65), Expect = 2.0
 Identities = 20/75 (26%), Positives = 34/75 (45%), Gaps = 3/75 (4%)

Query: 90  YYFSKFTEFMDTLFFVLRKKNEHVSTLHVIHHGIMPMSVWFGLKFAPGGHSTFFALLNTF 149
           +Y ++F     T+F VLR +   VS L    + +M  + +  L+F+   +     L  T 
Sbjct: 136 FYLTRFLHMFRTIFAVLRSRRLAVSQLFC--NSVMAFTSFLWLEFSQ-SYQILAILSTTL 192

Query: 150 VHIVMYFYYMVAAMG 164
           V+ V+Y Y      G
Sbjct: 193 VYSVVYGYRFWTGFG 207


>At3g27520.1 68416.m03440 expressed protein
          Length = 198

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 4/61 (6%)

Query: 229 RYTTAERKSKAQNGLCMAVMDDSSSSLNGRNGYKQEMGSEVPSSYASSGADALSFDVNKT 288
           R+ TA+R+ +      M+   +SSS+  G     +E G E  S   SS  D L F V + 
Sbjct: 95  RFQTAQREERLNANETMSFPGNSSSTTEG----GREFGGEDASKSPSSMLDQLLFTVERQ 150

Query: 289 E 289
           E
Sbjct: 151 E 151


>At4g31730.1 68417.m04504 expressed protein
          Length = 158

 Score = 28.3 bits (60), Expect = 7.9
 Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 2/61 (3%)

Query: 106 LRKKNEHVST-LHVIHHGIMPMSVWFG-LKFAPGGHSTFFALLNTFVHIVMYFYYMVAAM 163
           ++ K E V+T   V HHG+ P S W   + +  GG +    L+   + I+   Y+ +++ 
Sbjct: 6   VQSKFEDVATSTSVNHHGVTPQSPWHSPVPYLFGGLAAMLGLIAFALLILACSYWRLSSS 65

Query: 164 G 164
           G
Sbjct: 66  G 66


>At3g48050.2 68416.m05239 bromo-adjacent homology (BAH)
            domain-containing protein contains Pfam profile PF01426:
            BAH domain
          Length = 1613

 Score = 28.3 bits (60), Expect = 7.9
 Identities = 13/42 (30%), Positives = 22/42 (52%)

Query: 264  EMGSEVPSSYASSGADALSFDVNKTERDTQIISFTVNKPKRV 305
            ++ S V  S     +  L  D+NK +  T +IS+T+N   R+
Sbjct: 1289 QVRSGVMGSALDHSSGGLDLDLNKVDDSTDMISYTMNSSHRL 1330


>At3g48050.1 68416.m05238 bromo-adjacent homology (BAH)
            domain-containing protein contains Pfam profile PF01426:
            BAH domain
          Length = 1613

 Score = 28.3 bits (60), Expect = 7.9
 Identities = 13/42 (30%), Positives = 22/42 (52%)

Query: 264  EMGSEVPSSYASSGADALSFDVNKTERDTQIISFTVNKPKRV 305
            ++ S V  S     +  L  D+NK +  T +IS+T+N   R+
Sbjct: 1289 QVRSGVMGSALDHSSGGLDLDLNKVDDSTDMISYTMNSSHRL 1330


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.328    0.137    0.448 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,923,978
Number of Sequences: 28952
Number of extensions: 318498
Number of successful extensions: 936
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 928
Number of HSP's gapped (non-prelim): 7
length of query: 343
length of database: 12,070,560
effective HSP length: 82
effective length of query: 261
effective length of database: 9,696,496
effective search space: 2530785456
effective search space used: 2530785456
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 60 (28.3 bits)