Silkworm BLAST Result against bee

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000420-TA|BGIBMGA000420-PA|IPR000719|Protein kinase,
IPR011009|Protein kinase-like, IPR008271|Serine/threonine protein
kinase, active site, IPR002290|Serine/threonine protein kinase
         (484 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protei...    78   2e-16
AB013287-1|BAA87893.1|  190|Apis mellifera calmodulin kinase II ...    73   5e-15
AB183889-1|BAD86829.1|  316|Apis mellifera Mos protein.                60   4e-11
DQ013068-1|AAY81956.1|  931|Apis mellifera dusty protein kinase ...    58   1e-10
DQ013067-1|AAY81955.1|  969|Apis mellifera dusty protein kinase ...    58   1e-10
AB013288-1|BAA87894.1|  149|Apis mellifera protein kinase C prot...    52   1e-08
AY921579-1|AAX14899.1|  996|Apis mellifera ephrin receptor protein.    52   1e-08
AF514804-1|AAM51823.1|  537|Apis mellifera neuronal nicotinic ac...    25   1.4  

>AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protein
           kinase foraging protein.
          Length = 678

 Score = 77.8 bits (183), Expect = 2e-16
 Identities = 66/262 (25%), Positives = 110/262 (41%), Gaps = 16/262 (6%)

Query: 100 LGEGSYGKVKEMLDSQSLCRRAVXXXXXXXXXXXPNGEQNVQREIQLLRILRHKNVIELV 159
           LG G +G+V+ +  +    R                 +Q++  E +++       V++L 
Sbjct: 373 LGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIMSEKRIMGEADCDFVVKLF 432

Query: 160 DVIYNDEKQKMYLVMEFCVGVLQDMLESSPGKKFPQRQAHDYFLQLLDGLDYLHGQGVVH 219
              + D K  +Y++ME C+G     +    G  F       Y   +++  DYLH + +++
Sbjct: 433 KT-FKDRKY-LYMLMEACLGGELWTVLRDKGH-FDDGTTRFYTACVVEAFDYLHSRNIIY 489

Query: 220 KDIKPGNLLLTLDQTLKITDFGVAEALDMFSQEDICYTGQGSPAFQPPE-IANGAEQFSG 278
           +D+KP NLLL     +K+ DFG A+ LD   +    +T  G+P +  PE I N     S 
Sbjct: 490 RDLKPENLLLDSQGYVKLVDFGFAKRLDHGRK---TWTFCGTPEYVAPEVILNKGHDIS- 545

Query: 279 TKVDIWSSGVTLYNMTTGRYPFEGDNVYRLLEAISRXXXXXXXXXXXXXXXXXXXHMLNR 338
              D WS GV ++ + TG  PF G +  +    I +                     L R
Sbjct: 546 --ADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILKGIDAIEFPRSITRNATALIKKLCR 603

Query: 339 D-PTLR-----PTVHEIRRHAW 354
           D P  R       + EI++H W
Sbjct: 604 DNPAERLGYQKGGISEIQKHKW 625


>AB013287-1|BAA87893.1|  190|Apis mellifera calmodulin kinase II
           protein.
          Length = 190

 Score = 72.9 bits (171), Expect = 5e-15
 Identities = 48/169 (28%), Positives = 78/169 (46%), Gaps = 10/169 (5%)

Query: 193 FPQRQAHDYFLQLLDGLDYLHGQGVVHKDIKPGNLLL---TLDQTLKITDFGVAEALDMF 249
           + +  A     Q+L+ + + H  GVVH+D+KP NLLL        +K+ DFG+  A+++ 
Sbjct: 6   YSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGL--AIEVQ 63

Query: 250 SQEDICYTGQGSPAFQPPEIANGAEQFSGTKVDIWSSGVTLYNMTTGRYPFEGDNVYRLL 309
            +    +   G+P +  PE+    ++  G  VDIW+ GV LY +  G  PF  ++ +RL 
Sbjct: 64  GEAQAWFGFAGTPGYLSPEVLK--KEPYGKPVDIWACGVILYILLVGYPPFWDEDQHRLY 121

Query: 310 EAI---SRXXXXXXXXXXXXXXXXXXXHMLNRDPTLRPTVHEIRRHAWV 355
             I   S                     ML  +P+ R T  E  +H W+
Sbjct: 122 AQIKTGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRITASEALKHPWI 170


>AB183889-1|BAD86829.1|  316|Apis mellifera Mos protein.
          Length = 316

 Score = 60.1 bits (139), Expect = 4e-11
 Identities = 40/154 (25%), Positives = 76/154 (49%), Gaps = 12/154 (7%)

Query: 150 LRHKNVIELVDVIYNDEKQKMYLV-MEFCVGVLQDMLESSPGKKFPQRQAHDYFLQLLDG 208
           L+H N+++++ +   ++   + L+ ME C   LQ+ L+ +   K    +       +   
Sbjct: 114 LKHSNIVKVLMI---EQGASLSLITMELCGTTLQNRLDEAILIK---NERICILKSITCA 167

Query: 209 LDYLHGQGVVHKDIKPGNLLLTLDQTLKITDFGVAEALDMFSQEDICYTGQGSPAFQPPE 268
           L + H  G+VH D+KP N+L++ +   K+TDFG +  +   ++ D  Y   G+P +  PE
Sbjct: 168 LQFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFY---GTPGYTAPE 224

Query: 269 IANGAEQFSGTKVDIWSSGVTLYNMTTGRYPFEG 302
           +    +       DI+S G+  + M   + PF G
Sbjct: 225 VIK--QNRPTPAADIYSLGIVAWQMLFRKLPFAG 256


>DQ013068-1|AAY81956.1|  931|Apis mellifera dusty protein kinase
           isoform B protein.
          Length = 931

 Score = 58.4 bits (135), Expect = 1e-10
 Identities = 34/102 (33%), Positives = 56/102 (54%), Gaps = 14/102 (13%)

Query: 203 LQLLDGLDYLHGQGVVHKDIKPGNLLLTLDQTLKITDFGVAEALDMFSQEDICYTGQ--G 260
           L +L+G+ YLH QG+VH+D+K  N+LL ++   K+TDFG       F   ++   G   G
Sbjct: 704 LDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFG-------FCITEVMMLGSIVG 756

Query: 261 SPAFQPPEIANGAEQFSGTKVDIWSSGVTLYNMTTG--RYPF 300
           +P    PE+ +G    S   VD+++ G+  + +  G  R P+
Sbjct: 757 TPVHMAPELLSGHYDSS---VDVYAFGILFWYLCAGHVRLPY 795


>DQ013067-1|AAY81955.1|  969|Apis mellifera dusty protein kinase
           isoform A protein.
          Length = 969

 Score = 58.4 bits (135), Expect = 1e-10
 Identities = 34/102 (33%), Positives = 56/102 (54%), Gaps = 14/102 (13%)

Query: 203 LQLLDGLDYLHGQGVVHKDIKPGNLLLTLDQTLKITDFGVAEALDMFSQEDICYTGQ--G 260
           L +L+G+ YLH QG+VH+D+K  N+LL ++   K+TDFG       F   ++   G   G
Sbjct: 742 LDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFG-------FCITEVMMLGSIVG 794

Query: 261 SPAFQPPEIANGAEQFSGTKVDIWSSGVTLYNMTTG--RYPF 300
           +P    PE+ +G    S   VD+++ G+  + +  G  R P+
Sbjct: 795 TPVHMAPELLSGHYDSS---VDVYAFGILFWYLCAGHVRLPY 833


>AB013288-1|BAA87894.1|  149|Apis mellifera protein kinase C
           protein.
          Length = 149

 Score = 52.0 bits (119), Expect = 1e-08
 Identities = 28/76 (36%), Positives = 45/76 (59%), Gaps = 1/76 (1%)

Query: 169 KMYLVMEFCVGVLQDMLESSPGKKFPQRQAHDYFLQLLDGLDYLHGQGVVHKDIKPGNLL 228
           ++Y VME+  G    M +     KF +  A  Y  ++  GL +LHG+G+V++D+K  N+L
Sbjct: 59  RLYFVMEYVNGG-DLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVL 117

Query: 229 LTLDQTLKITDFGVAE 244
           L  D  +KI DFG+ +
Sbjct: 118 LDQDGHIKIADFGMCK 133


>AY921579-1|AAX14899.1|  996|Apis mellifera ephrin receptor protein.
          Length = 996

 Score = 51.6 bits (118), Expect = 1e-08
 Identities = 40/160 (25%), Positives = 68/160 (42%), Gaps = 6/160 (3%)

Query: 143 EIQLLRILRHKNVIELVDVIYNDEKQKMYLVMEFCV-GVLQDMLESSPGKKFPQRQAHDY 201
           E  ++    H NVI L  V+   +   + ++ EF   G L   L ++ GK F   Q    
Sbjct: 684 EASIMGQFEHPNVIFLQGVV--TKSNPVMIITEFMENGSLDTFLRANDGK-FQVLQLVGM 740

Query: 202 FLQLLDGLDYLHGQGVVHKDIKPGNLLLTLDQTLKITDFGVAEALDMFSQEDICYTGQGS 261
              +  G+ YL     VH+D+   N+L+      KI DFG++  ++  ++      G   
Sbjct: 741 LRGIASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKI 800

Query: 262 PAFQPPEIANGAEQFSGTKVDIWSSGVTLYN-MTTGRYPF 300
           P       A    +F+    D+WS G+  +  M+ G  P+
Sbjct: 801 PVRWTAPEAIAFRKFTSAS-DVWSMGIVCWEVMSYGERPY 839


>AF514804-1|AAM51823.1|  537|Apis mellifera neuronal nicotinic
           acetylcholine receptoralpha-3 protein.
          Length = 537

 Score = 25.0 bits (52), Expect = 1.4
 Identities = 11/32 (34%), Positives = 13/32 (40%)

Query: 267 PEIANGAEQFSGTKVDIWSSGVTLYNMTTGRY 298
           PE   G E       +IW   + LYN   G Y
Sbjct: 99  PEEYGGVEMLYVPSENIWLPDIVLYNNADGNY 130


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.318    0.136    0.406 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 131,986
Number of Sequences: 429
Number of extensions: 5205
Number of successful extensions: 16
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5
Number of HSP's gapped (non-prelim): 8
length of query: 484
length of database: 140,377
effective HSP length: 60
effective length of query: 424
effective length of database: 114,637
effective search space: 48606088
effective search space used: 48606088
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 45 (22.2 bits)