BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000410-TA|BGIBMGA000410-PA|undefined (519 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 31 0.29 SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 31 0.51 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 1.6 SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizo... 29 2.1 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 29 2.1 SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 28 3.6 SPBC1A4.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 3.6 SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces po... 28 3.6 SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharo... 28 3.6 SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 27 4.8 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 4.8 SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 27 6.3 SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr... 27 6.3 SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 27 8.3 >SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 818 Score = 31.5 bits (68), Expect = 0.29 Identities = 18/80 (22%), Positives = 39/80 (48%), Gaps = 2/80 (2%) Query: 395 YESAIKPSELTV--TNISKSNVTNNALKEISNVHWMRVDSGFKDENSADSIQFYPGNENS 452 ++S +P++LT SK++ ++A + SN+ ++ D N + I+ +P N+ Sbjct: 298 HKSNGRPNKLTAFFNKNSKASSISSAEEHPSNIDDSSIERRHYDSNHSSQIRDHPSTNNN 357 Query: 453 VKKFSNVEETEMKLANKEGT 472 + N ET + + + T Sbjct: 358 ASSYQNFNETSDEGEDNDAT 377 >SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 30.7 bits (66), Expect = 0.51 Identities = 13/46 (28%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Query: 6 EVTANGSLCRKLFFEEHSDIDHYDSEQTDDEIHAEPRPPSSYEAHT 51 ++ + +C + E+ +++DH DS+ DE+ A+ PP E T Sbjct: 258 DIPSEKIVCYNVIKEKSNEVDHKDSQHVVDEV-AQTSPPEVKEVET 302 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.1 bits (62), Expect = 1.6 Identities = 27/111 (24%), Positives = 46/111 (41%), Gaps = 1/111 (0%) Query: 359 GRATNDVTKVGDDCSTSFKRVDSGFNENTFYASTSYYESAIKPSELTVTNISKSNVTNNA 418 G +T+ VT TS R + F +T +++S + S + ++ SNVT ++ Sbjct: 627 GTSTSKVTSSTSIPLTSTNRTSTTFTSSTSISTSSSSTATSSTSFASESSSFYSNVTTSS 686 Query: 419 LKEISNVHWMRVDSGFKDEN-SADSIQFYPGNENSVKKFSNVEETEMKLAN 468 + S F ++ S+ P N VK S TE+K A+ Sbjct: 687 STVSTPPPTTSFPSTFTTSFITSSSLSSIPNNSTEVKTASTSSGTEIKTAS 737 >SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizosaccharomyces pombe|chr 2|||Manual Length = 468 Score = 28.7 bits (61), Expect = 2.1 Identities = 21/64 (32%), Positives = 27/64 (42%) Query: 386 NTFYASTSYYESAIKPSELTVTNISKSNVTNNALKEISNVHWMRVDSGFKDENSADSIQF 445 +T Y TSY + S T +S S+VTN+ E + GF D NS F Sbjct: 354 STPYCHTSYPAMSRLNSSSGYTCVSSSSVTNSGHTENNTWRSDEQSKGFVDINSFSQSLF 413 Query: 446 YPGN 449 GN Sbjct: 414 SNGN 417 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 28.7 bits (61), Expect = 2.1 Identities = 14/44 (31%), Positives = 23/44 (52%) Query: 373 STSFKRVDSGFNENTFYASTSYYESAIKPSELTVTNISKSNVTN 416 S+S K + T + +TS+ S+ +P+ T IS N+TN Sbjct: 163 SSSSKSSSRSSSRTTSHRTTSHKSSSYRPTVFPYTTISHYNITN 206 >SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 610 Score = 27.9 bits (59), Expect = 3.6 Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Query: 231 AHDQSQADSESSSHPDAQA--TWSIVEETNAQQAVRDGCIKLQ 271 +H +S++ S +SHPD QA TW E A + D + L+ Sbjct: 198 SHLKSKSRSRYASHPDLQALNTWMTNEGNQANDEMEDVSLYLE 240 >SPBC1A4.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 27.9 bits (59), Expect = 3.6 Identities = 24/113 (21%), Positives = 49/113 (43%), Gaps = 5/113 (4%) Query: 350 FLTENVSQYGRATNDVTKVGDDCSTSFKRVDSGFNE-NTFYASTSYYESAIKPSELTVTN 408 F N+SQ ++N+ + +F + NE +F S++ S ++ + Sbjct: 22 FCFRNLSQ---SSNNNVSYASSSNRNFVPQNVLNNEYQSFQHSSTSQPSVLRQGKNAFLK 78 Query: 409 ISKSNVTNNALKEISNVHWMRVDSGFKDENSADSIQFYPGNENSVKKFSNVEE 461 S+ + N+ EISN HW R + ++ S+ P + + F+N ++ Sbjct: 79 PSQLSFNMNS-SEISNTHWARDFNILTSNFASSSVTSAPTQSSHISNFTNSQK 130 >SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 27.9 bits (59), Expect = 3.6 Identities = 11/25 (44%), Positives = 16/25 (64%) Query: 138 DEEKNNFTSPESEAMATCLDCISDP 162 DE ++N S E +A A C+D S+P Sbjct: 68 DELESNLKSSEDQAYALCIDSFSNP 92 >SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 27.9 bits (59), Expect = 3.6 Identities = 20/89 (22%), Positives = 41/89 (46%) Query: 410 SKSNVTNNALKEISNVHWMRVDSGFKDENSADSIQFYPGNENSVKKFSNVEETEMKLANK 469 + +NV+ + ++E V D ++ +Q + NE+ K+ S V +++ A Sbjct: 63 ASANVSLSGVEEPVPVESNPSDQDVMSNSTEADLQLHTKNEHLEKRHSFVGKSDFPDAAV 122 Query: 470 EGTADDFDRFLVQSCNKDAMSMSDIFTDD 498 +G + F+ + K +S+ DI DD Sbjct: 123 QGDNTKNEDFVQSTPKKMDVSLEDISLDD 151 >SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 674 Score = 27.5 bits (58), Expect = 4.8 Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Query: 133 KVASSDEEKNNFTSPESEAMATCLDCISDPRSEEK 167 K ASS+EE F S E ++ T D +D SEE+ Sbjct: 195 KDASSEEENKEFVSDEGHSIVT-EDASADSESEEE 228 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 27.5 bits (58), Expect = 4.8 Identities = 23/98 (23%), Positives = 45/98 (45%), Gaps = 5/98 (5%) Query: 355 VSQYGRATNDVTKVGDDCSTSFKRVDSGFNEN-TFYASTSYYESAIKPSELTVTNISKSN 413 +SQ GR + ++ + D S V + N N T +++ +KP + + ++ Sbjct: 108 MSQSGRFSANLYEYVDGNSDGISFVLNLENNNDTSVYHMTFHGDRVKPINVF---LGSTD 164 Query: 414 VTNNALKEISNVHWMRVDSGFKDENSADSIQFYPGNEN 451 VT N + + W+ DS +KD D +++P +N Sbjct: 165 VTPN-FGGVDVIPWLLKDSPYKDAPPLDGTEYFPLLQN 201 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 27.1 bits (57), Expect = 6.3 Identities = 11/22 (50%), Positives = 14/22 (63%) Query: 349 KFLTENVSQYGRATNDVTKVGD 370 +FL+E QYGR D +VGD Sbjct: 113 RFLSERAKQYGRLGVDYAQVGD 134 >SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 27.1 bits (57), Expect = 6.3 Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Query: 383 FNENTFYASTSYYESAI-KPSELTVTNISKSNVTNNAL 419 +N + TS + S + KPS V+++S++N NAL Sbjct: 494 YNSSPVQTRTSLFSSRLSKPSNPIVSSVSQANAPKNAL 531 >SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosaccharomyces pombe|chr 1|||Manual Length = 875 Score = 26.6 bits (56), Expect = 8.3 Identities = 23/98 (23%), Positives = 41/98 (41%), Gaps = 1/98 (1%) Query: 365 VTKVGDDCSTSFKRVDSGFNENTFYASTSYYESAIKPSELTVTNISKSNVTNNALKEISN 424 V DD + S NEN S + ++K S S+++ N+ +IS Sbjct: 591 VDNANDDTDQAPTTNSSSLNENLLGGHASENDKSLKQSPFQKLTRRLSDLSVNSSGQISK 650 Query: 425 VHWMRVDSGFKDENSADSIQFYPGN-ENSVKKFSNVEE 461 + +S F + + + I P + +K F++VEE Sbjct: 651 QDFDNSNSIFSESSLSSPIIEEPKTLIDCLKNFTHVEE 688 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.312 0.126 0.361 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,230,966 Number of Sequences: 5004 Number of extensions: 89244 Number of successful extensions: 230 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 224 Number of HSP's gapped (non-prelim): 14 length of query: 519 length of database: 2,362,478 effective HSP length: 76 effective length of query: 443 effective length of database: 1,982,174 effective search space: 878103082 effective search space used: 878103082 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.9 bits) S2: 56 (26.6 bits)
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