BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000408-TA|BGIBMGA000408-PA|IPR000719|Protein kinase, IPR013543|Calcium/calmodulin dependent protein kinase II, association-domain, IPR011009|Protein kinase-like, IPR008271|Serine/threonine protein kinase, active site, IPR002290|Serine/threonine protein kinase (510 letters) Database: mosquito 2123 sequences; 516,269 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CR954256-9|CAJ14150.1| 872|Anopheles gambiae putative calcium/c... 225 3e-60 AJ301655-1|CAC35008.1| 1433|Anopheles gambiae putative epidermal... 47 1e-06 AY578807-1|AAT07312.1| 438|Anopheles gambiae punt protein. 46 3e-06 AY578808-1|AAT07313.1| 458|Anopheles gambiae saxophone protein. 42 5e-05 AY578798-1|AAT07303.1| 356|Anopheles gambiae baboon protein. 39 3e-04 AY578811-1|AAT07316.1| 565|Anopheles gambiae thickveins protein. 36 0.002 AY505417-1|AAR90328.1| 206|Anopheles gambiae superoxide dismuta... 27 1.6 AJ441131-7|CAD29636.1| 1977|Anopheles gambiae putative Tyr/Ser/T... 25 3.6 AJ010299-1|CAA09070.1| 722|Anopheles gambiae stat protein. 25 4.8 AF515527-1|AAM61894.1| 211|Anopheles gambiae glutathione S-tran... 25 4.8 >CR954256-9|CAJ14150.1| 872|Anopheles gambiae putative calcium/calmodulin-dependentprotein kinase, CAKI protein. Length = 872 Score = 225 bits (549), Expect = 3e-60 Identities = 120/292 (41%), Positives = 172/292 (58%), Gaps = 10/292 (3%) Query: 26 GAFSIVRRAVQKSTGYEFAAKIINTKKLSAR---DFQKLEREARICRKLQHPNIVRLHDS 82 G FSIVRR + + + +FA KI++ K +A L+REA IC L+HP+IV L ++ Sbjct: 1 GPFSIVRRCIHRESNQQFAVKIVDVAKFTASPGLSTSDLKREATICHMLKHPHIVELLET 60 Query: 83 IQEEHCHYLVFDLVTGGELFEDI---VAREFYSEADASHCIQQILESVHHCHHNGVVHRD 139 E Y+VFD+ FE + VA YSEA A H ++QILE++ +CH N ++HRD Sbjct: 61 YSSEGMLYMVFDMEGSDICFEVVRRAVAGFVYSEAVACHYLRQILEALRYCHENDIIHRD 120 Query: 140 LKPENLLLASKAKGAAVKLADFGLAIEVQG--DQQAWFGFAGTPGYLSPEVLKKEPYGKP 197 ++P LLA+ A VKL FG A+++ D G G P Y++PEV+ + YGKP Sbjct: 121 VRPACALLATADNSAPVKLGGFGSAVQLPNGRDSVETHGRVGCPHYMAPEVVARRVYGKP 180 Query: 198 VDIWACGVILYILLVGYPPFWDEDQYRLYAQIKAGAYDYPSPEWDTVTPEAKSLINQMLT 257 D+W GV+L++LL G PF + RL I G +PEW ++ AK L+ +ML Sbjct: 181 CDVWGAGVMLHVLLSGRLPFHGSGK-RLQDAIARGRVTLDTPEWKHISSNAKDLVLKMLA 239 Query: 258 VNPSKRITASEALKHPWICHRERVASVMHRQETVDCLKKFNARRKLKGAILT 309 NP R T +E L HPWI R+++ + H +TV+ LK++NARRKLK A+ T Sbjct: 240 PNPISRPTITEVLDHPWIRDRDKLQRI-HLGDTVEELKRYNARRKLKAAVQT 290 >AJ301655-1|CAC35008.1| 1433|Anopheles gambiae putative epidermal growth factor receptorprotein. Length = 1433 Score = 46.8 bits (106), Expect = 1e-06 Identities = 52/203 (25%), Positives = 89/203 (43%), Gaps = 13/203 (6%) Query: 23 LGKGAFSIVRRAVQKSTGYEF----AAKIINTKKLSARDFQKLEREARICRKLQHPNIVR 78 LG GAF V + V G A K++ S + LE EA I ++HPN+++ Sbjct: 840 LGMGAFGRVFKGVWMPEGESVKIPVAIKVLMEMSGSESSKEFLE-EAYIMASVEHPNLLK 898 Query: 79 LHDSIQEEHCHYLVFDLVTGGELFEDIVAREFYSEADAS-HCIQQILESVHHCHHNGVVH 137 L L+ L+ G L + + + + A + QI + + +VH Sbjct: 899 LLAVCMTSQM-MLITQLMPLGCLLDYVRNNKDKIGSKALLNWSTQIARGMAYLEERRLVH 957 Query: 138 RDLKPENLLLASKAKGAAVKLADFGLA--IEVQGDQQAWFGFAGTPGYLSPEVLKKEPYG 195 RDL N+L+ + + VK+ FGLA ++ D+ G +L+ E ++ + Sbjct: 958 RDLAARNVLVQTP---SCVKITVFGLAKLLDFDSDEYRAAGGKMPIKWLALECIRHRVFT 1014 Query: 196 KPVDIWACGVILYILLV-GYPPF 217 D+WA G+ ++ LL G P+ Sbjct: 1015 SKSDVWAFGITIWELLTYGARPY 1037 >AY578807-1|AAT07312.1| 438|Anopheles gambiae punt protein. Length = 438 Score = 45.6 bits (103), Expect = 3e-06 Identities = 65/256 (25%), Positives = 108/256 (42%), Gaps = 35/256 (13%) Query: 4 PNRESVSTRFSDN-YELKEELGKGAFSIVRRAVQKSTGYEFAAKIINTKKLSARDFQKLE 62 P+ + ST S +LK+ +G F +V RA + E A KI + R E Sbjct: 106 PDISNSSTNISHRPIDLKDIKARGRFGVVWRA--QLGNQEVAVKIF---PMQERQSWITE 160 Query: 63 REARICRKLQHPNIVRLHDSIQEEHCHYLVFDLVTG----GELFEDIVAREFYSEADASH 118 ++ ++ HPNI+ + F L+T G L D + S + Sbjct: 161 QDIFKLPRMNHPNILEFIGCEKRSDMASTDFWLITAYCENGSLC-DFLKAHTVSWTELCK 219 Query: 119 CIQQILESVHHCHHN-----------GVVHRDLKPENLLLASKAKGAAVKLADFGLAIEV 167 + + H H + HRD K +N+LL KA A +ADFGLA+ Sbjct: 220 IATTMARGLTHLHEEIQSSRTDGLKPSIAHRDFKSKNVLL--KADLTAC-IADFGLALVF 276 Query: 168 QGDQQAW--FGFAGTPGYLSPEVLK------KEPYGKPVDIWACGVILYILLVGYPPFWD 219 + G GT Y++PEVL+ ++ + + +D++ACG++L+ L+ Sbjct: 277 TPGKSCGDTHGQVGTRRYMAPEVLEGAINFTRDAFLR-IDVYACGLVLWELVSRCTVHGG 335 Query: 220 E-DQYRLYAQIKAGAY 234 D+YRL + + G + Sbjct: 336 PVDEYRLPFEAELGPH 351 >AY578808-1|AAT07313.1| 458|Anopheles gambiae saxophone protein. Length = 458 Score = 41.5 bits (93), Expect = 5e-05 Identities = 59/212 (27%), Positives = 91/212 (42%), Gaps = 31/212 (14%) Query: 19 LKEELGKGAFSIVRRAVQKSTGYEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVR 78 L E +G+G + V R + G A KI ++ D K E E L+H NI+ Sbjct: 153 LCECIGRGRYGEVWRGIWH--GESVAVKIFFSRD---EDSWKRETEIYGTVLLRHENILG 207 Query: 79 L--HDSIQEEHCH--YLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVHHCHHN- 133 D C +L+ G LF+ + + + C+ I + H H Sbjct: 208 YVGSDMTSRNSCTQLWLITHYYPQGSLFDYLNRTAISTHQMITICLS-IANGMVHLHTEI 266 Query: 134 -------GVVHRDLKPENLLLASKAKGAAVKLADFGLAI-EVQGDQQAWFG---FAGTPG 182 + HRDLK +N+L+ +A G V +ADFGLA+ Q + G GT Sbjct: 267 FGTEGKPAIAHRDLKTKNILI--RANGTCV-IADFGLAVMHSQTTNKIDIGNTARVGTKR 323 Query: 183 YLSPEVLKK----EPYG--KPVDIWACGVILY 208 Y++PEVL + E + + DI+A G+I + Sbjct: 324 YMAPEVLDESISMECFDALRKADIYAIGLIFW 355 >AY578798-1|AAT07303.1| 356|Anopheles gambiae baboon protein. Length = 356 Score = 39.1 bits (87), Expect = 3e-04 Identities = 59/215 (27%), Positives = 92/215 (42%), Gaps = 35/215 (16%) Query: 18 ELKEELGKGAFSIVRRAVQKSTGYEFAAKIINTKKLSARDFQKLEREARICRK--LQHPN 75 +L + +GKG F V R + G A KI S+R+ REA I + L+H N Sbjct: 60 QLVDVIGKGRFGEVWRG--RWRGENVAVKIF-----SSREECSWSREAEIYQTIMLRHEN 112 Query: 76 IVRLHDSIQEEHCHY----LVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVHHCH 131 I+ + +++ + LV D G LF+ + AR + I + H H Sbjct: 113 ILGFIAADNKDNGTWTQLWLVTDYHENGSLFDFLTARCVDPDTMLEMAFS-IATGLAHLH 171 Query: 132 HN--------GVVHRDLKPENLLLASKAKGAAVKLADFGLAIE--VQGD--QQAWFGFAG 179 + + HRDLK +N+L+ S + D GLA+ V D Q G Sbjct: 172 MDIVGTRGKPAIAHRDLKSKNILVKS---NLTCCIGDLGLAVRHIVATDTVDQPSTHRVG 228 Query: 180 TPGYLSPEVLKKE------PYGKPVDIWACGVILY 208 T Y++PEVL + K D++A G++L+ Sbjct: 229 TKRYMAPEVLDETINVSQFDSFKRADVYALGLVLW 263 >AY578811-1|AAT07316.1| 565|Anopheles gambiae thickveins protein. Length = 565 Score = 36.3 bits (80), Expect = 0.002 Identities = 22/61 (36%), Positives = 35/61 (57%), Gaps = 7/61 (11%) Query: 135 VVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGD----QQAWFGFAGTPGYLSPEVLK 190 + HRD+K +N+L+ + A +ADFGLA++ + Q A GT Y++PEVL Sbjct: 383 IAHRDIKSKNILVKRNGQCA---IADFGLAVKYTSESDTIQIANNSRVGTRRYMAPEVLS 439 Query: 191 K 191 + Sbjct: 440 E 440 >AY505417-1|AAR90328.1| 206|Anopheles gambiae superoxide dismutase 1 protein. Length = 206 Score = 26.6 bits (56), Expect = 1.6 Identities = 16/49 (32%), Positives = 22/49 (44%) Query: 121 QQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQG 169 ++I+E H HHN V E L + AK K+ G AI+ G Sbjct: 52 REIMELHHQKHHNAYVTNLNAAEEQLQDAVAKQDVSKIIQLGNAIKFNG 100 >AJ441131-7|CAD29636.1| 1977|Anopheles gambiae putative Tyr/Ser/Thr phosphatase protein. Length = 1977 Score = 25.4 bits (53), Expect = 3.6 Identities = 12/33 (36%), Positives = 16/33 (48%) Query: 241 WDTVTPEAKSLINQMLTVNPSKRITASEALKHP 273 W TV + + + V PS + A EA KHP Sbjct: 1605 WRTVRQLLERTRQKRMAVCPSSVVLAREAFKHP 1637 >AJ010299-1|CAA09070.1| 722|Anopheles gambiae stat protein. Length = 722 Score = 25.0 bits (52), Expect = 4.8 Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 3/56 (5%) Query: 437 HK-FFIDNTPPHVKTNTTILNPRVHLLGDDVAV--IAYVCVTQSVDSEGRRATHQS 489 HK F ++N PP V T V LL D+ + I VT S+ SE + QS Sbjct: 289 HKAFIVENQPPQVMKMNTRFCASVRLLIDNALIMKIGNPKVTVSIISETQAQQIQS 344 >AF515527-1|AAM61894.1| 211|Anopheles gambiae glutathione S-transferase D10 protein. Length = 211 Score = 25.0 bits (52), Expect = 4.8 Identities = 12/40 (30%), Positives = 19/40 (47%) Query: 411 YSKLCDPNVTAFDPDALGNLIEGVEFHKFFIDNTPPHVKT 450 Y P +TA+ G L + EFHK + + ++KT Sbjct: 171 YDLAAFPGITAWVARVTGELPDYGEFHKELYEKSMEYIKT 210 Database: mosquito Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 516,269 Number of sequences in database: 2123 Lambda K H 0.319 0.134 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 515,096 Number of Sequences: 2123 Number of extensions: 21372 Number of successful extensions: 41 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 28 Number of HSP's gapped (non-prelim): 11 length of query: 510 length of database: 516,269 effective HSP length: 67 effective length of query: 443 effective length of database: 374,028 effective search space: 165694404 effective search space used: 165694404 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 50 (24.2 bits)
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