BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000408-TA|BGIBMGA000408-PA|IPR000719|Protein kinase, IPR013543|Calcium/calmodulin dependent protein kinase II, association-domain, IPR011009|Protein kinase-like, IPR008271|Serine/threonine protein kinase, active site, IPR002290|Serine/threonine protein kinase (510 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 402 e-114 AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 116 5e-28 AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 93 7e-21 DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 62 1e-11 DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 62 1e-11 AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 59 1e-10 AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 56 7e-10 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 25 1.1 >AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II protein. Length = 190 Score = 402 bits (991), Expect = e-114 Identities = 182/190 (95%), Positives = 187/190 (98%) Query: 106 VAREFYSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAI 165 VAREFYSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAI Sbjct: 1 VAREFYSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAI 60 Query: 166 EVQGDQQAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQYRL 225 EVQG+ QAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQ+RL Sbjct: 61 EVQGEAQAWFGFAGTPGYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQHRL 120 Query: 226 YAQIKAGAYDYPSPEWDTVTPEAKSLINQMLTVNPSKRITASEALKHPWICHRERVASVM 285 YAQIK G+YDYPSPEWDTVTPEAK+LINQMLTVNPSKRITASEALKHPWIC RERVASV+ Sbjct: 121 YAQIKTGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRITASEALKHPWICQRERVASVV 180 Query: 286 HRQETVDCLK 295 HRQETVDCLK Sbjct: 181 HRQETVDCLK 190 >AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein kinase foraging protein. Length = 678 Score = 116 bits (279), Expect = 5e-28 Identities = 82/279 (29%), Positives = 133/279 (47%), Gaps = 18/279 (6%) Query: 5 NRESVSTRFSDNYELKEELGKGAFSIVRRA-VQKSTGYEFAAKIINTKKL-SARDFQKLE 62 N E R D L LG G F V + + FA K + ++ R Q + Sbjct: 356 NEEFRDLRLQDLRPLAT-LGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQQHIM 414 Query: 63 REARICRKLQHPNIVRLHDSIQEEHCHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQ 122 E RI + +V+L + ++ Y++ + GGEL+ + + + + Sbjct: 415 SEKRIMGEADCDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGHFDDGTTRFYTAC 474 Query: 123 ILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDQQAWFGFAGTPG 182 ++E+ + H +++RDLKPENLLL S+ VKL DFG A + ++ W F GTP Sbjct: 475 VVEAFDYLHSRNIIYRDLKPENLLLDSQGY---VKLVDFGFAKRLDHGRKTWT-FCGTPE 530 Query: 183 YLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQYRLYAQIKAG--AYDYPSPE 240 Y++PEV+ + + D W+ GV+++ LL G PPF D + Y I G A ++P Sbjct: 531 YVAPEVILNKGHDISADYWSLGVLMFELLTGTPPFTGGDPMKTYNIILKGIDAIEFPR-- 588 Query: 241 WDTVTPEAKSLINQMLTVNPSKRI-----TASEALKHPW 274 ++T A +LI ++ NP++R+ SE KH W Sbjct: 589 --SITRNATALIKKLCRDNPAERLGYQKGGISEIQKHKW 625 >AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. Length = 316 Score = 92.7 bits (220), Expect = 7e-21 Identities = 76/269 (28%), Positives = 127/269 (47%), Gaps = 18/269 (6%) Query: 4 PNRESVSTRFSDNYELK--EELGKGAFSIVRRAVQKSTGYEFAAKIINTKKLSARDFQKL 61 PNR+ + D + +K LG G F IV +A+ K G + AAKII T+K S + Sbjct: 55 PNRQKI---LKDGFPIKCGTFLGSGGFGIVYKALYK--GEQVAAKIIQTEKYS--NMLNS 107 Query: 62 EREARICRKLQHPNIVRLHDSIQEEHCHYLVFDLVTGGELFEDIVAREFYSEADASHCIQ 121 E+ A L+H NIV++ Q + +L G ++ + + + ++ Sbjct: 108 EKHASF---LKHSNIVKVLMIEQGASLSLITMELC--GTTLQNRLDEAILIKNERICILK 162 Query: 122 QILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDQQAWFGFAGTP 181 I ++ CH+ G+VH D+KP+N+L++ K KL DFG ++ + G F GTP Sbjct: 163 SITCALQFCHNAGIVHADVKPKNILMS---KNGQPKLTDFGSSVLI-GAPNEIDKFYGTP 218 Query: 182 GYLSPEVLKKEPYGKPVDIWACGVILYILLVGYPPFWDEDQYRLYAQIKAGAYDYPSPEW 241 GY +PEV+K+ DI++ G++ + +L PF + + G Sbjct: 219 GYTAPEVIKQNRPTPAADIYSLGIVAWQMLFRKLPFAGLHSHTIIYLSAKGHRPIDDNID 278 Query: 242 DTVTPEAKSLINQMLTVNPSKRITASEAL 270 D K+L QM + N ++R T +E + Sbjct: 279 DEFKGTYKTLYKQMWSQNITERPTTNEVI 307 >DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase isoform B protein. Length = 931 Score = 62.1 bits (144), Expect = 1e-11 Identities = 58/202 (28%), Positives = 91/202 (45%), Gaps = 17/202 (8%) Query: 21 EELGKGAFSIVRRAVQKSTGYEFAAKIINTKKLSARDFQKLEREARICRKL-QHPNIVRL 79 EE+G+G + IV A G I + + L E R + H IV+L Sbjct: 598 EEIGRGQYGIVF-ACDGWGGKAGPCAIKSVVPSDESHWNDLAMEFYYNRSIPDHKRIVKL 656 Query: 80 HDSIQEEHCHYLVFDLVTGGELFEDIVAREFYSEADAS----HCIQ---QILESVHHCHH 132 SI + H + F + L D ++R+ Y A IQ +LE + + H Sbjct: 657 RGSIID-HSYGGGFGFGSAVLLISDRLSRDLYCGIRAGLSWLERIQIALDVLEGIRYLHS 715 Query: 133 NGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLKKE 192 G+VHRD+K +N+LL + + KL DFG I + GTP +++PE+L Sbjct: 716 QGLVHRDVKLKNVLLDIENR---AKLTDFGFCIT---EVMMLGSIVGTPVHMAPELLSGH 769 Query: 193 PYGKPVDIWACGVILYILLVGY 214 Y VD++A G++ + L G+ Sbjct: 770 -YDSSVDVYAFGILFWYLCAGH 790 >DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase isoform A protein. Length = 969 Score = 62.1 bits (144), Expect = 1e-11 Identities = 58/202 (28%), Positives = 91/202 (45%), Gaps = 17/202 (8%) Query: 21 EELGKGAFSIVRRAVQKSTGYEFAAKIINTKKLSARDFQKLEREARICRKL-QHPNIVRL 79 EE+G+G + IV A G I + + L E R + H IV+L Sbjct: 636 EEIGRGQYGIVF-ACDGWGGKAGPCAIKSVVPSDESHWNDLAMEFYYNRSIPDHKRIVKL 694 Query: 80 HDSIQEEHCHYLVFDLVTGGELFEDIVAREFYSEADAS----HCIQ---QILESVHHCHH 132 SI + H + F + L D ++R+ Y A IQ +LE + + H Sbjct: 695 RGSIID-HSYGGGFGFGSAVLLISDRLSRDLYCGIRAGLSWLERIQIALDVLEGIRYLHS 753 Query: 133 NGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLKKE 192 G+VHRD+K +N+LL + + KL DFG I + GTP +++PE+L Sbjct: 754 QGLVHRDVKLKNVLLDIENR---AKLTDFGFCIT---EVMMLGSIVGTPVHMAPELLSGH 807 Query: 193 PYGKPVDIWACGVILYILLVGY 214 Y VD++A G++ + L G+ Sbjct: 808 -YDSSVDVYAFGILFWYLCAGH 828 >AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. Length = 996 Score = 58.8 bits (136), Expect = 1e-10 Identities = 39/181 (21%), Positives = 82/181 (45%), Gaps = 9/181 (4%) Query: 64 EARICRKLQHPNIVRLHDSIQEEHCHYLVFDLVTGGELFEDIVARE-FYSEADASHCIQQ 122 EA I + +HPN++ L + + + ++ + + G L + A + + ++ Sbjct: 684 EASIMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRG 743 Query: 123 ILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGDQQAWFGFAG--- 179 I + + VHRDL N+L+ + K+ADFGL+ E++ + + G Sbjct: 744 IASGMQYLAEMNYVHRDLAARNVLVNA---ALVCKIADFGLSREIESATEGAYTTRGGKI 800 Query: 180 TPGYLSPEVLKKEPYGKPVDIWACGVILY-ILLVGYPPFWDEDQYRLYAQIKAGAYDYPS 238 + +PE + + D+W+ G++ + ++ G P+W+ + I+ G Y P+ Sbjct: 801 PVRWTAPEAIAFRKFTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKG-YRLPA 859 Query: 239 P 239 P Sbjct: 860 P 860 Score = 27.9 bits (59), Expect = 0.21 Identities = 12/37 (32%), Positives = 19/37 (51%) Query: 215 PPFWDEDQYRLYAQIKAGAYDYPSPEWDTVTPEAKSL 251 PP W+ D Y+L +I AG + + + E KS+ Sbjct: 125 PPPWETDSYKLIGRIAAGEGRFNTNTEVVINTEVKSI 161 >AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C protein. Length = 149 Score = 56.0 bits (129), Expect = 7e-10 Identities = 46/151 (30%), Positives = 70/151 (46%), Gaps = 7/151 (4%) Query: 34 AVQKSTGYEFAAKIINTKKLSARDFQKLER-EARICR-KLQHPNIVRLHDSIQEEHCHYL 91 A +K T +A KI+ + D + E R+ + P +V+LH Q Y Sbjct: 3 AERKGTDELYAIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLYF 62 Query: 92 VFDLVTGGELFEDIVAREFYSEADASHCIQQILESVHHCHHNGVVHRDLKPENLLLASKA 151 V + V GG+L I + E A +I + H G+V+RDLK +N+LL Sbjct: 63 VMEYVNGGDLMYQIQQCGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLL---D 119 Query: 152 KGAAVKLADFGLAIE-VQGDQQAWFGFAGTP 181 + +K+ADFG+ E + GD+ F GTP Sbjct: 120 QDGHIKIADFGMCKEGISGDKTT-KTFCGTP 149 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 25.4 bits (53), Expect = 1.1 Identities = 11/30 (36%), Positives = 14/30 (46%) Query: 478 VDSEGRRATHQSQETRIWHKRHNKWTAVHF 507 VD + + T T+IW H KW A F Sbjct: 45 VDEKNQILTTNCWVTQIWTDHHLKWNASEF 74 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.319 0.134 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 139,644 Number of Sequences: 429 Number of extensions: 5555 Number of successful extensions: 19 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 2 Number of HSP's gapped (non-prelim): 9 length of query: 510 length of database: 140,377 effective HSP length: 61 effective length of query: 449 effective length of database: 114,208 effective search space: 51279392 effective search space used: 51279392 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 46 (22.6 bits)
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