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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000403-TA|BGIBMGA000403-PA|IPR001873|Na+ channel,
amiloride-sensitive
         (749 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_23646| Best HMM Match : ASC (HMM E-Value=1.4e-12)                   36   0.12 
SB_3565| Best HMM Match : ASC (HMM E-Value=1.1e-12)                    36   0.12 
SB_19561| Best HMM Match : No HMM Matches (HMM E-Value=.)              36   0.12 
SB_15223| Best HMM Match : ASC (HMM E-Value=1.8e-19)                   36   0.12 
SB_42305| Best HMM Match : DM (HMM E-Value=0.47)                       34   0.36 
SB_46636| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   1.9  
SB_2653| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   2.6  
SB_52562| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   3.4  
SB_36224| Best HMM Match : ASC (HMM E-Value=1.9e-38)                   31   4.5  
SB_31018| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   4.5  
SB_3195| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   4.5  
SB_22423| Best HMM Match : ASC (HMM E-Value=1.8e-05)                   30   7.8  

>SB_23646| Best HMM Match : ASC (HMM E-Value=1.4e-12)
          Length = 885

 Score = 35.9 bits (79), Expect = 0.12
 Identities = 41/187 (21%), Positives = 69/187 (36%), Gaps = 14/187 (7%)

Query: 462 DFFLSCKWNGRPIDCCKQFKPLATEYG-TCFSINNKNVAGTQIPLVATSNRRIPGELEIT 520
           D    C W        K F    +  G TC++ N+    G + P+++ SN      L + 
Sbjct: 233 DMIKECSWQKHGKCSWKNFTSFKSADGDTCYTFNS----GRKDPILSMSNVGEENGLRLV 288

Query: 521 TSQDYEAFIHSPEDVPFWNMEYDRRIRIV--YGSQATVTFT-IMDVVNEPEVSLIAPETR 577
               +  + +  ++  F  + +D+    V   G   +  FT  M++      +L  P   
Sbjct: 289 IDTQHSEYYYDVKNAGFKVILHDQGETPVKMQGLSVSPGFTSYMELKRTKVTNLPFPYKT 348

Query: 578 QCRFSDETPENILAFKHYSYSVCITQCRIDAQLRMCNCTHHLSPIEFKDHVCDFE-GLKC 636
            C      PE +  F  YS S C         + +C C     P + K  VCD+E    C
Sbjct: 349 MCGM----PE-LKYFNSYSKSKCFLDKLTQYVVTLCGCRDWFMPGQGKIPVCDYETAASC 403

Query: 637 LTNNYKT 643
           +   + T
Sbjct: 404 MWKAWAT 410


>SB_3565| Best HMM Match : ASC (HMM E-Value=1.1e-12)
          Length = 575

 Score = 35.9 bits (79), Expect = 0.12
 Identities = 45/185 (24%), Positives = 73/185 (39%), Gaps = 19/185 (10%)

Query: 462 DFFLSCKWNGRPIDCCKQFKPLATE-----YGTCFSINN-KNVAGTQIPLVATSNRRIPG 515
           D  LSC + G P  C    + L +      YG C+  N+ K+  G   P++  SN+  P 
Sbjct: 121 DMVLSCTYRGIP--CTNYTENLWSRFWHYRYGNCYVFNSGKDWQGEPKPILR-SNKPGPA 177

Query: 516 E---LEITTSQ-DYEAFI-HSPEDVPFWNMEYDRRIRIVYGSQATVTFTIMDVVNEPEVS 570
               LE+   Q +Y   + H        + + +    +  G   +  F+    +    + 
Sbjct: 178 SGLTLELNAEQHEYVGQLSHEAGMRVLISTQGEMPSPLEKGISVSPGFSTTTGIRMTNIL 237

Query: 571 LIAP-ETRQCRFSDET-PENILAFKH---YSYSVCITQCRIDAQLRMCNCTHHLSPIEFK 625
              P   + C  SD+  PEN+   ++   YS + C+  C    QL MC C  +  P    
Sbjct: 238 RADPFNNKSCLSSDDIDPENLYKKRYNVSYSRTTCMDSCLAHKQLDMCGCLEYRFPNIDN 297

Query: 626 DHVCD 630
             VCD
Sbjct: 298 RTVCD 302


>SB_19561| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 410

 Score = 35.9 bits (79), Expect = 0.12
 Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 3/36 (8%)

Query: 461 KDFFLSCKWNGRPIDCCKQFKPLAT-EYGTCFSINN 495
           KDF   C W G+P  C   F+ + T EYG CF+ N+
Sbjct: 247 KDFIFGCYWLGQP--CNTSFEQIFTPEYGNCFTFNS 280


>SB_15223| Best HMM Match : ASC (HMM E-Value=1.8e-19)
          Length = 605

 Score = 35.9 bits (79), Expect = 0.12
 Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 13/98 (13%)

Query: 577 RQCRFSDET-PENILAFKH---YSYSVCITQCRIDAQLRMCNCTHHLSPIEFKDHVC--- 629
           + C  SD+  PEN+   ++   YS + C+  C    Q+ MC C  +  P      VC   
Sbjct: 184 KSCLSSDDIDPENLYRKRYNVSYSRTTCMDSCLAHKQVDMCGCLEYRFPNIDNRTVCDIL 243

Query: 630 DFEGLKCLTNNYKTLGKLKVPGTNEAGLVCNCLPSCIE 667
           D + ++CL N  ++L +      N+ G    C P C E
Sbjct: 244 DIDVIRCL-NKVQSLYQ-----DNKLGCSEKCRPPCNE 275


>SB_42305| Best HMM Match : DM (HMM E-Value=0.47)
          Length = 161

 Score = 34.3 bits (75), Expect = 0.36
 Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 1/53 (1%)

Query: 592 FKHYSYSVCITQCRIDAQLRMCNCTHHLSPIEFKDHVCDFE-GLKCLTNNYKT 643
           FK Y+ S C+ +CR D  ++MC C  +     F++   + E  + C    Y+T
Sbjct: 6   FKKYTKSACLLKCRADYVMKMCKCRSYDLKEIFRNESINCECPVPCEITKYQT 58


>SB_46636| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 55

 Score = 31.9 bits (69), Expect = 1.9
 Identities = 16/36 (44%), Positives = 22/36 (61%)

Query: 195 NSTRTSTPQRSSLEGTNSKSKKVSFFRRLGRSKDST 230
           +S R S P+RSSL G   +S++V+  R L  S D T
Sbjct: 6   SSVRRSCPERSSLYGATDRSRRVAVTRMLQGSLDYT 41


>SB_2653| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1298

 Score = 31.5 bits (68), Expect = 2.6
 Identities = 19/71 (26%), Positives = 28/71 (39%), Gaps = 1/71 (1%)

Query: 435 CLATCDFKPICSSDFTQTASMFRTPCKDFFLSCKWNGRPIDCCKQFKPLATEYGTCFSIN 494
           CL+ C F   C+         F  P +DF  +  +   P+  CK   P +T Y  C    
Sbjct: 882 CLS-CPFGASCAGYIKANDGFFGYPVQDFPPALNFTVCPVGYCKSPPPYSTMYNACSGSR 940

Query: 495 NKNVAGTQIPL 505
           +      +IPL
Sbjct: 941 SAASELERIPL 951


>SB_52562| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1490

 Score = 31.1 bits (67), Expect = 3.4
 Identities = 23/66 (34%), Positives = 31/66 (46%), Gaps = 5/66 (7%)

Query: 214  SKKVSFFRRLGRSKDSTPPRRTSSFT-----MQQPSVEMALRELTHPIHNEQLKNQQQVT 268
            SKK S  R L R +D  PP+ TSS T       Q   +++  ELT     E+ +  Q  +
Sbjct: 1207 SKKRSTRRSLDRKQDKNPPQTTSSKTSTPAEKPQEQHKLSWAELTEEAEAEEARVHQAES 1266

Query: 269  FKLVKT 274
             K  KT
Sbjct: 1267 LKQGKT 1272


>SB_36224| Best HMM Match : ASC (HMM E-Value=1.9e-38)
          Length = 451

 Score = 30.7 bits (66), Expect = 4.5
 Identities = 39/150 (26%), Positives = 62/150 (41%), Gaps = 21/150 (14%)

Query: 596 SYSVCITQCRIDAQLRMCNCTHHLSPIE----FKDHVCDFEGLKCLTNNYKT-LGKLKVP 650
           S S C+ QC  D  ++ C C H L   E    F+ ++C    ++C    +   L  L  P
Sbjct: 282 SQSYCLVQCYSDMVVKRCGC-HMLGMTEETGRFQPNMCSCP-VRCSRVEFDVQLSSLYYP 339

Query: 651 GTNEAGLVCNCLPSCIEPDYNVISKKLSEPEKFLRARPSSFIMGSRPYQRVTRQV----- 705
             N    + N      E    + +    + +++ R R    I  +  Y+ +T +V     
Sbjct: 340 PDNFWETISN------ERSLTIYANDTKKFQEWYRRR---VIQLNVFYKELTTEVRKEKE 390

Query: 706 ARTTLDLVVAMGNCFGLCFGGSLLSIAEVV 735
           A T  DL    G   GL  G S+L+IAE +
Sbjct: 391 AYTISDLAGDFGGNMGLFLGCSILTIAEFI 420


>SB_31018| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1066

 Score = 30.7 bits (66), Expect = 4.5
 Identities = 19/69 (27%), Positives = 30/69 (43%), Gaps = 2/69 (2%)

Query: 444 ICSSDFTQTASMFRTPCKDFFLSCKWNGRPIDCCKQFKPLATEYGTCFS--INNKNVAGT 501
           I  +D  Q+   ++   ++    C+WN  PI       PLATE     S       V  T
Sbjct: 323 IIENDIAQSVQRYQLAVQEAKHDCRWNDSPIAVSPLPTPLATEESDLPSDVTITAIVVAT 382

Query: 502 QIPLVATSN 510
             P+++T+N
Sbjct: 383 ANPIISTNN 391


>SB_3195| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 650

 Score = 30.7 bits (66), Expect = 4.5
 Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 2/46 (4%)

Query: 487 YGTCFSINNKNVAGTQIPLVATSNRRIPGELEITTSQDYEAFIHSP 532
           Y  C  I+N+ V   Q+P   +  +  PG+ +++T Q  +   HSP
Sbjct: 259 YNLCRGIDNEPVRARQLPKSVSCGKNFPGKTKLSTCQ--QGASHSP 302


>SB_22423| Best HMM Match : ASC (HMM E-Value=1.8e-05)
          Length = 348

 Score = 29.9 bits (64), Expect = 7.8
 Identities = 11/21 (52%), Positives = 12/21 (57%)

Query: 595 YSYSVCITQCRIDAQLRMCNC 615
           YS S CI QC  D  +  CNC
Sbjct: 187 YSLSACIKQCLYDYGMEKCNC 207


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.323    0.136    0.423 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,491,254
Number of Sequences: 59808
Number of extensions: 1012754
Number of successful extensions: 2207
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2200
Number of HSP's gapped (non-prelim): 14
length of query: 749
length of database: 16,821,457
effective HSP length: 88
effective length of query: 661
effective length of database: 11,558,353
effective search space: 7640071333
effective search space used: 7640071333
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 64 (29.9 bits)

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