BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000397-TA|BGIBMGA000397-PA|IPR001478|PDZ/DHR/GLGF
(228 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48060.1 68418.m05938 C2 domain-containing protein contains I... 31 0.49
At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619 C... 31 0.49
At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive eff... 31 0.49
At1g10130.1 68414.m01142 calcium-transporting ATPase 3, endoplas... 30 1.5
At5g55540.1 68418.m06919 expressed protein 29 2.0
At5g46390.2 68418.m05710 peptidase S41 family protein similar to... 29 2.6
At5g46390.1 68418.m05709 peptidase S41 family protein similar to... 29 2.6
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 29 2.6
At5g55660.1 68418.m06940 expressed protein similar to unknown pr... 29 3.4
At3g12545.1 68416.m01561 lipid transfer protein-related low simi... 29 3.4
At4g08097.1 68417.m01316 hypothetical protein 28 4.5
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 28 4.5
At1g32490.1 68414.m04009 RNA helicase, putative similar to ATP-d... 28 4.5
At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 ... 28 6.0
At1g17450.1 68414.m02137 ATP phosphoribosyltransferase -related ... 28 6.0
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 27 7.9
At3g25130.1 68416.m03138 expressed protein 27 7.9
At3g16460.2 68416.m02097 jacalin lectin family protein contains ... 27 7.9
At3g16460.1 68416.m02098 jacalin lectin family protein contains ... 27 7.9
At2g47010.2 68415.m05873 expressed protein 27 7.9
At2g47010.1 68415.m05872 expressed protein 27 7.9
At2g44900.1 68415.m05589 armadillo/beta-catenin repeat family pr... 27 7.9
>At5g48060.1 68418.m05938 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 1036
Score = 31.5 bits (68), Expect = 0.49
Identities = 37/145 (25%), Positives = 62/145 (42%), Gaps = 9/145 (6%)
Query: 63 HIEVGDHIEK--LNGENMVGKRHYEVAKIL-KDIPKGTTFIMRLVEPLKSGFGSIGPKTG 119
HIEV + E+ + G + +G+ + I+ KD F + L S G IG K
Sbjct: 71 HIEVSVYHERRPIPGRSFLGRVKISLCNIVYKDDQVYQRFTLEKKWLLSSVKGEIGLKFY 130
Query: 120 SGRSGKKQNFGSGKETLRFKANGQATIENEHDDKSKAGIEKINGLLESFMGINDSELASQ 179
S + Q F + + + QA+ +D + + E L+SF + +LA
Sbjct: 131 ISSSEEDQTFPLPSKP--YTSPTQASASGTEEDTADSETEDS---LKSFASAEEEDLADS 185
Query: 180 MWDLAEGKQNSMELAEAIDNSDLQE 204
+ + EGK+ S E+ E + QE
Sbjct: 186 VSECVEGKK-SEEVKEPVQKLHRQE 209
>At5g46210.1 68418.m05686 cullin, putative similar to SP|Q13619
Cullin homolog 4A (CUL-4A) {Homo sapiens}; contains Pfam
profile PF00888: Cullin family
Length = 792
Score = 31.5 bits (68), Expect = 0.49
Identities = 29/98 (29%), Positives = 41/98 (41%), Gaps = 6/98 (6%)
Query: 126 KQNFGSGKETLRFKANGQATIENEHDDKSKAGIEKINGLLESFMGINDSELASQMWDLAE 185
K +F GK+ L QA + +D K E I + I D EL + LA
Sbjct: 612 KADFSKGKKELAVSLF-QAVVLMLFNDAMKLSFEDI----KDSTSIEDKELRRTLQSLAC 666
Query: 186 GKQNSMEL-AEAIDNSDLQEFGFTDEFIIELWGVITDA 222
GK ++ + D D EF F DEF L+ + +A
Sbjct: 667 GKVRVLQKNPKGRDVEDGDEFEFNDEFAAPLYRIKVNA 704
>At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1311
Score = 31.5 bits (68), Expect = 0.49
Identities = 27/81 (33%), Positives = 34/81 (41%), Gaps = 10/81 (12%)
Query: 85 EVAKILKD--IPKGTTFIMRLVEPLKSGFGSIGPKTGS------GRSGKKQNFGSGKETL 136
E+ + KD +PK +FI + P GF S GP+T S RSG T
Sbjct: 1208 EMESLPKDFPVPKVPSFIPKA--PNARGFRSGGPRTRSIDMHPESRSGTPSEDDKKLSTT 1265
Query: 137 RFKANGQATIENEHDDKSKAG 157
F NG + EN DD G
Sbjct: 1266 TFPRNGNSRRENSVDDSDPPG 1286
>At1g10130.1 68414.m01142 calcium-transporting ATPase 3, endoplasmic
reticulum-type (ACA6) (ECA3) nearly identical to
SP|Q9SY55 Calcium-transporting ATPase 3, endoplasmic
reticulum-type (EC 3.6.3.8) {Arabidopsis thaliana);
contains InterPro Accession IPR006069: Cation
transporting ATPase
Length = 998
Score = 29.9 bits (64), Expect = 1.5
Identities = 26/64 (40%), Positives = 36/64 (56%), Gaps = 10/64 (15%)
Query: 98 TFIMRLVEPLKSGFGSIGPKTGSGRSGKKQNFGS-GKETLR-----FKA--NGQATI--E 147
+ I R + L +G GS+ P T +GR+ + F S G ETLR FK +GQ TI +
Sbjct: 516 SIIARCNKILCNGDGSVVPLTAAGRAELESRFYSFGDETLRCLALAFKTVPHGQQTISYD 575
Query: 148 NEHD 151
NE+D
Sbjct: 576 NEND 579
>At5g55540.1 68418.m06919 expressed protein
Length = 1380
Score = 29.5 bits (63), Expect = 2.0
Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Query: 164 LLESFMGINDSELASQMWDLAEGKQNSMELAEAID-NSDLQEFGFTD 209
LL S + +NDS Q+W+ + G + + EL+ I+ NS L+ F D
Sbjct: 153 LLGSALQVNDSLEELQIWEDSIGSKGAEELSRMIEMNSSLKLFSIFD 199
>At5g46390.2 68418.m05710 peptidase S41 family protein similar to
C-terminal peptidase of the D1 protein [Hordeum vulgare
subsp vulgare] GI:1296805; contains Pfam profiles
PF03572: Peptidase family S41B, PF00595: PDZ domain
(Also known as DHR or GLGF)
Length = 489
Score = 29.1 bits (62), Expect = 2.6
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Query: 61 IPHIEVGDHIEKLNGENMVGKRHYEVAKILKDIPKGTTFIMRLVEPLKSG 110
I ++ GD I +NG ++ GK +EV+ +L+ K TF++ V+ K G
Sbjct: 229 IAGVKQGDEILAVNGMDVSGKSSFEVSSLLQGPSK--TFVVLKVKHGKCG 276
>At5g46390.1 68418.m05709 peptidase S41 family protein similar to
C-terminal peptidase of the D1 protein [Hordeum vulgare
subsp vulgare] GI:1296805; contains Pfam profiles
PF03572: Peptidase family S41B, PF00595: PDZ domain
(Also known as DHR or GLGF)
Length = 428
Score = 29.1 bits (62), Expect = 2.6
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Query: 61 IPHIEVGDHIEKLNGENMVGKRHYEVAKILKDIPKGTTFIMRLVEPLKSG 110
I ++ GD I +NG ++ GK +EV+ +L+ K TF++ V+ K G
Sbjct: 229 IAGVKQGDEILAVNGMDVSGKSSFEVSSLLQGPSK--TFVVLKVKHGKCG 276
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 29.1 bits (62), Expect = 2.6
Identities = 34/130 (26%), Positives = 56/130 (43%), Gaps = 8/130 (6%)
Query: 16 FAHRKGRPKEIEIVKTEDALGLTITDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEKLNG 75
F R K++ V ++ LG ++GA + + E S VS HI+ DHI K+
Sbjct: 258 FQPRTEFEKKMASVLKDNELGEAHKEDGAK---LLELNEASSVSMEDHIKYRDHIAKMRS 314
Query: 76 ENMVGKRHYEVAKILKDIPKGTTFIMRLVEPLKSGFGSIGPKTGSGRSGKKQNFGSGKE- 134
+ RH +K +K I T ++ + KS G++ + KQ +E
Sbjct: 315 ---LLFRHELKSKRIKKIKSKTYHRLKGKDLKKSAMGALMDPEMAKEEAIKQETRRVEER 371
Query: 135 -TLRFKANGQ 143
TL+ K G+
Sbjct: 372 MTLKHKNTGK 381
>At5g55660.1 68418.m06940 expressed protein similar to unknown
protein (pir||T08929)
Length = 778
Score = 28.7 bits (61), Expect = 3.4
Identities = 21/55 (38%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Query: 103 LVEPLKSGFGSIGPKTGSGRSGKK-QNFGSGKETLRFKANGQATIEN-EHDDKSK 155
+VEP +G S+ KT SGK+ Q SGKE K +E E DD+ K
Sbjct: 9 IVEPTANGTSSL-QKTSDAISGKEVQENASGKEVQESKKEEDTGLEKMEIDDEGK 62
>At3g12545.1 68416.m01561 lipid transfer protein-related low
similarity to Lipid transfer protein [Brassica rapa]
GI:3062791; contains Pfam profile PF00152: tRNA
synthetases class II (D, K and N)
Length = 123
Score = 28.7 bits (61), Expect = 3.4
Identities = 12/26 (46%), Positives = 18/26 (69%)
Query: 72 KLNGENMVGKRHYEVAKILKDIPKGT 97
K+NG+ M+G+ +E+A L I KGT
Sbjct: 56 KVNGDEMMGRCMHEIANCLVAIDKGT 81
>At4g08097.1 68417.m01316 hypothetical protein
Length = 346
Score = 28.3 bits (60), Expect = 4.5
Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 2/52 (3%)
Query: 43 GAGYAFIKRIKEGSIVSRIPHIEVGDHIEKLNGENMVGKRHYEVA--KILKD 92
G I IK SI ++P I D + LN EN++ H A K++KD
Sbjct: 284 GVPITLIPTIKYNSIQPKVPLIVNNDPVMVLNDENVLETAHEPSAEQKLIKD 335
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/54 (24%), Positives = 30/54 (55%), Gaps = 4/54 (7%)
Query: 20 KGRPKEIEIVKTEDALGLTITDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEKL 73
+ P+++ ++ AL LT++ + F+K I G ++R+ + V D +E++
Sbjct: 173 RSNPQKLAVI----ALNLTLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEI 222
>At1g32490.1 68414.m04009 RNA helicase, putative similar to
ATP-dependent RNA helicase #3 [Homo sapiens] GI:3107913;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1044
Score = 28.3 bits (60), Expect = 4.5
Identities = 27/74 (36%), Positives = 36/74 (48%), Gaps = 12/74 (16%)
Query: 132 GKETLRFKA-NGQATIENEHDDKSKAGIEKINGLLESFMGINDSELASQMWDLAEGKQNS 190
GK TL+F A N QA+ DD ++IN + ES M + E D + KQ S
Sbjct: 337 GKATLKFGAKNKQAS-----DDYQFVFEDQINFIKESVMAGENYE------DAMDAKQKS 385
Query: 191 MELAEAIDNSDLQE 204
+LAE +LQE
Sbjct: 386 QDLAEKTALEELQE 399
>At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1
subunit-related contains similarity to Swiss-Prot:P80387
5'-AMP-activated protein kinase, beta-1 subunit (AMPK
beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa]
Length = 591
Score = 27.9 bits (59), Expect = 6.0
Identities = 15/45 (33%), Positives = 23/45 (51%)
Query: 28 IVKTEDALGLTITDNGAGYAFIKRIKEGSIVSRIPHIEVGDHIEK 72
+V E LG+ + G F+ IK+GS + I VGD ++K
Sbjct: 78 MVTLEKPLGIRFALSADGKIFVHAIKKGSNAEKARIIMVGDTLKK 122
>At1g17450.1 68414.m02137 ATP phosphoribosyltransferase -related
contains weak similarity to Swiss-Prot:P10366 ATP
phosphoribosyltransferase [Escherichia coli]
Length = 1402
Score = 27.9 bits (59), Expect = 6.0
Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 5/56 (8%)
Query: 55 GSIVSRIPHIEVGDHIEKLNGENMVGKRHYEVAKILKDIPKGTTFIMRLVEPLKSG 110
G I RI E+G L G+N+ ++ E+ IL DI +G T + KSG
Sbjct: 265 GEIHDRIRSFELG-----LRGQNLSKRKSNELIPILNDIQRGQTNVDLDARASKSG 315
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 27.5 bits (58), Expect = 7.9
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 6/79 (7%)
Query: 18 HRKGRPKEIEIVKTEDALGLTITDNGAGYAFIKRI--KEGSIVSRIPHIEVGDHIEKLNG 75
H G P++IEI D +GL I G G IK + + G+ IP GD +++
Sbjct: 266 HAIGIPEQIEIKVPNDKVGLII---GRGGETIKNMQTRSGARTQLIPQHAEGDGLKERTV 322
Query: 76 ENMVGKRHYEVA-KILKDI 93
K ++A ++KD+
Sbjct: 323 RISGDKMQIDIATDMIKDV 341
>At3g25130.1 68416.m03138 expressed protein
Length = 406
Score = 27.5 bits (58), Expect = 7.9
Identities = 20/74 (27%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
Query: 134 ETLRFKANGQATIEN-EHDDKSKAGIEKINGLLESFMGINDSELASQMWDLAEGKQNSME 192
E + K ++N E K ++ +K++ + +S D S EGK+N +
Sbjct: 203 EEQKVKHKSDVVLDNREEPTKEESKAQKVDLVGDSNNESYDLPKLSNFLGEGEGKRNVVT 262
Query: 193 LAEAIDNSDLQEFG 206
E DN LQ FG
Sbjct: 263 KNEEEDNVSLQSFG 276
>At3g16460.2 68416.m02097 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 647
Score = 27.5 bits (58), Expect = 7.9
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Query: 109 SGFGSIGPKTGSGRSGKKQNFGSGKETLRFKANGQATIENEHDDKSK-AGIEKI 161
+G G+ G TG+G +G G+G + +A G +T DD S G+ KI
Sbjct: 227 TGTGT-GTGTGTGGTGTGTGTGTGSGAQKLEAQGNSTGGTSWDDGSDYDGVTKI 279
>At3g16460.1 68416.m02098 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 705
Score = 27.5 bits (58), Expect = 7.9
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Query: 109 SGFGSIGPKTGSGRSGKKQNFGSGKETLRFKANGQATIENEHDDKSK-AGIEKI 161
+G G+ G TG+G +G G+G + +A G +T DD S G+ KI
Sbjct: 227 TGTGT-GTGTGTGGTGTGTGTGTGSGAQKLEAQGNSTGGTSWDDGSDYDGVTKI 279
>At2g47010.2 68415.m05873 expressed protein
Length = 451
Score = 27.5 bits (58), Expect = 7.9
Identities = 12/34 (35%), Positives = 19/34 (55%)
Query: 80 GKRHYEVAKILKDIPKGTTFIMRLVEPLKSGFGS 113
G HY+ +LK +P+ F +RL +K G G+
Sbjct: 269 GGYHYDTRGMLKTLPESPNFKVRLTLNVKQGGGA 302
>At2g47010.1 68415.m05872 expressed protein
Length = 451
Score = 27.5 bits (58), Expect = 7.9
Identities = 12/34 (35%), Positives = 19/34 (55%)
Query: 80 GKRHYEVAKILKDIPKGTTFIMRLVEPLKSGFGS 113
G HY+ +LK +P+ F +RL +K G G+
Sbjct: 269 GGYHYDTRGMLKTLPESPNFKVRLTLNVKQGGGA 302
>At2g44900.1 68415.m05589 armadillo/beta-catenin repeat family
protein / F-box family protein contains similarity to
F-box protein FBL2 GI:6010699 from [Rattus norvegicus];
contains Pfam profiles PF00514:
Armadillo/beta-catenin-like repeat, PF00646: F-box
domain
Length = 930
Score = 27.5 bits (58), Expect = 7.9
Identities = 12/42 (28%), Positives = 25/42 (59%)
Query: 155 KAGIEKINGLLESFMGINDSELASQMWDLAEGKQNSMELAEA 196
+ GI+ + GL +S + E A +W+L+ G+++ +A+A
Sbjct: 478 EGGIKILAGLAKSMNRLVAEEAAGGLWNLSVGEEHKNAIAQA 519
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.136 0.385
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,574,366
Number of Sequences: 28952
Number of extensions: 247035
Number of successful extensions: 550
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 534
Number of HSP's gapped (non-prelim): 32
length of query: 228
length of database: 12,070,560
effective HSP length: 79
effective length of query: 149
effective length of database: 9,783,352
effective search space: 1457719448
effective search space used: 1457719448
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 58 (27.5 bits)
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