BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000387-TA|BGIBMGA000387-PA|IPR001611|Leucine-rich repeat, IPR002126|Cadherin, IPR000225|Armadillo, IPR003591|Leucine-rich repeat, typical subtype (2095 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 105 5e-24 DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 27 2.1 AJ517411-1|CAD56944.1| 1770|Apis mellifera vitellogenin precurso... 26 2.8 >AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor protein. Length = 1370 Score = 105 bits (251), Expect = 5e-24 Identities = 73/221 (33%), Positives = 108/221 (48%), Gaps = 4/221 (1%) Query: 4 NHVEYLPGGIFESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLFA 63 N + + G L L+T N L+ + F ST+ L + L+ N L LP+ +F Sbjct: 247 NAIVEIAGDALTGLTVLRTFNASYNSLDSLPEGLFASTRDLREIHLAYNGLRDLPKGIFT 306 Query: 64 NNTFLETIDISNNRV--VYVPSDSFLGLYSLQILDLSKNKIQNIQNGTF-SLKNLQILKL 120 L ++++ NR+ V +FLGL L +L+LS N + +I F L LQIL L Sbjct: 307 RLEQLLVLNLAGNRLGSDRVDETTFLGLIRLIVLNLSYNMLTHIDARMFKDLFFLQILDL 366 Query: 121 SDNKINNISDNAFENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGVE 180 +N I+ I NAF NKL+ + QLFN L L L LS N + S+ + Sbjct: 367 RNNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFNGLFVLNRLTLSGNAIASIDPLA 426 Query: 181 FESLKLLRNLDLKENALSQIPDNTFSDCFNLEKLDLSKNKL 221 F + L+ LDL N L+ +PD D L+ LDL +N++ Sbjct: 427 FRNCSDLKELDLSGNELTSVPD-ALRDLALLKTLDLGENRI 466 Score = 103 bits (248), Expect = 1e-23 Identities = 73/229 (31%), Positives = 113/229 (49%), Gaps = 4/229 (1%) Query: 4 NHVEYLPGGIFESLVNLKTLRLQRNYL--EEIDSEAFTSTKKLFHVDLSNNYLYTLPEKL 61 N + LP GIF L L L L N L + +D F +L ++LS N L + ++ Sbjct: 295 NGLRDLPKGIFTRLEQLLVLNLAGNRLGSDRVDETTFLGLIRLIVLNLSYNMLTHIDARM 354 Query: 62 FANNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTFS-LKNLQILKL 120 F + FL+ +D+ NN + + S++FL LY+L L+LS NK++ + F+ L L L L Sbjct: 355 FKDLFFLQILDLRNNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFNGLFVLNRLTL 414 Query: 121 SDNKINNISDNAFENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGVE 180 S N I +I AF N N+L ++P L +L L LDL N + + Sbjct: 415 SGNAIASIDPLAFRNCSDLKELDLSGNELTSVPDAL-RDLALLKTLDLGENRISNFYNGS 473 Query: 181 FESLKLLRNLDLKENALSQIPDNTFSDCFNLEKLDLSKNKLRFINITAF 229 F +L L L L N + + D NL+ L+L++NK++ + AF Sbjct: 474 FRNLDQLTGLRLIGNDIGNLSRGMLWDLPNLQILNLARNKVQHVERYAF 522 Score = 101 bits (241), Expect = 8e-23 Identities = 84/278 (30%), Positives = 126/278 (45%), Gaps = 4/278 (1%) Query: 17 LVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLFANNTFLETIDISNN 76 L L+ L LQRN + EI +A T L + S N L +LPE LFA+ L I ++ N Sbjct: 236 LRQLQELHLQRNAIVEIAGDALTGLTVLRTFNASYNSLDSLPEGLFASTRDLREIHLAYN 295 Query: 77 RVVYVPSDSFLGLYSLQILDLSKNKI--QNIQNGTF-SLKNLQILKLSDNKINNISDNAF 133 + +P F L L +L+L+ N++ + TF L L +L LS N + +I F Sbjct: 296 GLRDLPKGIFTRLEQLLVLNLAGNRLGSDRVDETTFLGLIRLIVLNLSYNMLTHIDARMF 355 Query: 134 ENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGVEFESLKLLRNLDLK 193 ++ N + I + F L L L+LS+N L ++ F L +L L L Sbjct: 356 KDLFFLQILDLRNNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFNGLFVLNRLTLS 415 Query: 194 ENALSQIPDNTFSDCFNLEKLDLSKNKLRFINITAFYGXXXXXXXXXXXXXXYEVHFKAF 253 NA++ I F +C +L++LDLS N+L + A + +F Sbjct: 416 GNAIASIDPLAFRNCSDLKELDLSGNELTSVP-DALRDLALLKTLDLGENRISNFYNGSF 474 Query: 254 SMLKNLTTLYLDGNMFPSLPSRTLDYMPKLTIVKLSGN 291 L LT L L GN +L L +P L I+ L+ N Sbjct: 475 RNLDQLTGLRLIGNDIGNLSRGMLWDLPNLQILNLARN 512 Score = 86.6 bits (205), Expect = 2e-18 Identities = 59/222 (26%), Positives = 108/222 (48%), Gaps = 1/222 (0%) Query: 4 NHVEYLPGGIFESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLFA 63 N + ++ +F+ L L+ L L+ N ++ I+S AF L ++LS+N L T+ +LF Sbjct: 345 NMLTHIDARMFKDLFFLQILDLRNNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFN 404 Query: 64 NNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTFSLKNLQILKLSDN 123 L + +S N + + +F L+ LDLS N++ ++ + L L+ L L +N Sbjct: 405 GLFVLNRLTLSGNAIASIDPLAFRNCSDLKELDLSGNELTSVPDALRDLALLKTLDLGEN 464 Query: 124 KINNISDNAFENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGVEFES 183 +I+N + +F N N + N+ + +L L L+L+ N + + FE Sbjct: 465 RISNFYNGSFRNLDQLTGLRLIGNDIGNLSRGMLWDLPNLQILNLARNKVQHVERYAFER 524 Query: 184 LKLLRNLDLKENALSQIPDNTFSDCFNLEKLDLSKNKLRFIN 225 L + L N LS I + F+ +L L+LS+N + + + Sbjct: 525 NMRLEAIRLDGNFLSDI-NGVFTSIASLLLLNLSENHIEWFD 565 Score = 85.4 bits (202), Expect = 4e-18 Identities = 67/218 (30%), Positives = 103/218 (47%), Gaps = 5/218 (2%) Query: 3 DNHVEYLPGGIFESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLF 62 +N ++ + F L NL TL L N L + ++ F L + LS N + ++ F Sbjct: 368 NNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFNGLFVLNRLTLSGNAIASIDPLAF 427 Query: 63 ANNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTF-SLKNLQILKLS 121 N + L+ +D+S N + VP D+ L L+ LDL +N+I N NG+F +L L L+L Sbjct: 428 RNCSDLKELDLSGNELTSVP-DALRDLALLKTLDLGENRISNFYNGSFRNLDQLTGLRLI 486 Query: 122 DNKINNISDNAFENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGVEF 181 N I N+S + RNK++++ F L + L N L + GV F Sbjct: 487 GNDIGNLSRGMLWDLPNLQILNLARNKVQHVERYAFERNMRLEAIRLDGNFLSDINGV-F 545 Query: 182 ESLKLLRNLDLKENALSQIPDNTFSDCFNLEKLDLSKN 219 S+ L L+L EN + D F NL+ LD+ N Sbjct: 546 TSIASLLLLNLSENHIEWF-DYAFIP-GNLKWLDIHGN 581 Score = 75.4 bits (177), Expect = 5e-15 Identities = 62/213 (29%), Positives = 95/213 (44%), Gaps = 2/213 (0%) Query: 14 FESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLFANNTFLETIDI 73 F L+ L L L N L ID+ F L +DL NN + + F L T+++ Sbjct: 331 FLGLIRLIVLNLSYNMLTHIDARMFKDLFFLQILDLRNNSIDRIESNAFLPLYNLHTLEL 390 Query: 74 SNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTF-SLKNLQILKLSDNKINNISDNA 132 S+N++ V + F GL+ L L LS N I +I F + +L+ L LS N++ ++ D A Sbjct: 391 SDNKLRTVGAQLFNGLFVLNRLTLSGNAIASIDPLAFRNCSDLKELDLSGNELTSVPD-A 449 Query: 133 FENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGVEFESLKLLRNLDL 192 + N++ N F NL L L L N++ +L+ L L+ L+L Sbjct: 450 LRDLALLKTLDLGENRISNFYNGSFRNLDQLTGLRLIGNDIGNLSRGMLWDLPNLQILNL 509 Query: 193 KENALSQIPDNTFSDCFNLEKLDLSKNKLRFIN 225 N + + F LE + L N L IN Sbjct: 510 ARNKVQHVERYAFERNMRLEAIRLDGNFLSDIN 542 Score = 70.9 bits (166), Expect = 1e-13 Identities = 79/311 (25%), Positives = 132/311 (42%), Gaps = 28/311 (9%) Query: 9 LPGGIFESLVNLKTLRLQR-------NYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKL 61 +P G F+ L+ LK L +Q + E+ ++F ++L +++ + + LP Sbjct: 107 IPEGAFQPLLELKKLTVQTFNSVWGASRFLELAPDSFLGLRELHTLEIVESNVQALPVNS 166 Query: 62 FANNTFLETIDISNNRVVYV------------PSDSFLGLYS-----LQILDLSKNKIQN 104 + L+T++++ NR+ + SD G S ++ILDLS+N+I Sbjct: 167 LCSLDNLQTLNLTENRLRDINDIGLNRRDSDDGSDGNDGDESSCRADIRILDLSRNEITR 226 Query: 105 IQNGT--FSLKNLQILKLSDNKINNISDNAFENXXXXXXXXXDRNKLKNIPTQLFNNLKC 162 +Q + L+ LQ L L N I I+ +A N L ++P LF + + Sbjct: 227 LQENSPLLDLRQLQELHLQRNAIVEIAGDALTGLTVLRTFNASYNSLDSLPEGLFASTRD 286 Query: 163 LIFLDLSNNNLMSLAGVEFESLKLLRNLDLKENAL--SQIPDNTFSDCFNLEKLDLSKNK 220 L + L+ N L L F L+ L L+L N L ++ + TF L L+LS N Sbjct: 287 LREIHLAYNGLRDLPKGIFTRLEQLLVLNLAGNRLGSDRVDETTFLGLIRLIVLNLSYNM 346 Query: 221 LRFINITAFYGXXXXXXXXXXXXXXYEVHFKAFSMLKNLTTLYLDGNMFPSLPSRTLDYM 280 L I+ F + AF L NL TL L N ++ ++ + + Sbjct: 347 LTHIDARMFKDLFFLQILDLRNNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFNGL 406 Query: 281 PKLTIVKLSGN 291 L + LSGN Sbjct: 407 FVLNRLTLSGN 417 Score = 53.6 bits (123), Expect = 2e-08 Identities = 41/151 (27%), Positives = 61/151 (40%), Gaps = 1/151 (0%) Query: 168 LSNNNLMSLAGVEFESLKLLRNLDLKENALSQIPDNTFSDCFNLEKLDLSKNKLRFINIT 227 L N L L F K +R L + + + I + TF+ NL+ L L N++R + Sbjct: 801 LDGNVLRELQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELKGF 860 Query: 228 AFYGXXXXXXXXXXXXXXYEVHFKAFSMLKNLTTLYLDGNMFPSLPSRTLDYMPKLTIVK 287 F + F L++L L L GN + P + +L + Sbjct: 861 EFERLSHLRELYLQNNLIGFIGNLTFLPLRSLEILRLSGNRLVTFPVWQVTLNARLVELS 920 Query: 288 LSGNPWHCDCHALY-ISAWVRLNEMKIWDYS 317 L NPW C C L +S+WV N K+ D S Sbjct: 921 LGSNPWSCRCKFLQELSSWVSDNAHKVVDAS 951 Score = 50.4 bits (115), Expect = 2e-07 Identities = 32/102 (31%), Positives = 49/102 (48%) Query: 120 LSDNKINNISDNAFENXXXXXXXXXDRNKLKNIPTQLFNNLKCLIFLDLSNNNLMSLAGV 179 L N + + ++ F + + +++I + FN L L L L +N + L G Sbjct: 801 LDGNVLRELQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELKGF 860 Query: 180 EFESLKLLRNLDLKENALSQIPDNTFSDCFNLEKLDLSKNKL 221 EFE L LR L L+ N + I + TF +LE L LS N+L Sbjct: 861 EFERLSHLRELYLQNNLIGFIGNLTFLPLRSLEILRLSGNRL 902 Score = 50.4 bits (115), Expect = 2e-07 Identities = 31/91 (34%), Positives = 48/91 (52%), Gaps = 1/91 (1%) Query: 86 FLGLYSLQILDLSKNKIQNIQNGTFS-LKNLQILKLSDNKINNISDNAFENXXXXXXXXX 144 F+G ++++L ++ + I++IQN TF+ L NLQIL L DN+I + FE Sbjct: 814 FIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELKGFEFERLSHLRELYL 873 Query: 145 DRNKLKNIPTQLFNNLKCLIFLDLSNNNLMS 175 N + I F L+ L L LS N L++ Sbjct: 874 QNNLIGFIGNLTFLPLRSLEILRLSGNRLVT 904 Score = 41.9 bits (94), Expect = 5e-05 Identities = 30/118 (25%), Positives = 57/118 (48%), Gaps = 1/118 (0%) Query: 4 NHVEYLPGGIFESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLFA 63 N + L +F N++ L + + +E I + F L + L +N + L F Sbjct: 804 NVLRELQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELKGFEFE 863 Query: 64 NNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTFSLKNLQILKLS 121 + L + + NN + ++ + +FL L SL+IL LS N++ +L N ++++LS Sbjct: 864 RLSHLRELYLQNNLIGFIGNLTFLPLRSLEILRLSGNRLVTFPVWQVTL-NARLVELS 920 Score = 41.5 bits (93), Expect = 7e-05 Identities = 32/131 (24%), Positives = 65/131 (49%), Gaps = 6/131 (4%) Query: 1 MFDNHVEYLPGGIFESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEK 60 + N + L G+ L NL+ L L RN ++ ++ AF +L + L N+L + Sbjct: 485 LIGNDIGNLSRGMLWDLPNLQILNLARNKVQHVERYAFERNMRLEAIRLDGNFLSDI-NG 543 Query: 61 LFANNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTFSLKNLQI--L 118 +F + L +++S N + + G +L+ LD+ N I+++ N + +++ ++ L Sbjct: 544 VFTSIASLLLLNLSENHIEWFDYAFIPG--NLKWLDIHGNFIESLGN-YYKIRDSKVKTL 600 Query: 119 KLSDNKINNIS 129 S N+I +S Sbjct: 601 DASHNRITELS 611 Score = 41.1 bits (92), Expect = 9e-05 Identities = 34/127 (26%), Positives = 58/127 (45%), Gaps = 3/127 (2%) Query: 47 VDLSNNYLYTLPEKLFANNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQ 106 V L N L L +F + + ++ + + + + +F GL +LQIL L N+I+ ++ Sbjct: 799 VYLDGNVLRELQNHVFIGRKNMRVLYVNGSGIESIQNRTFNGLNNLQILHLEDNRIRELK 858 Query: 107 NGTFS-LKNLQILKLSDNKINNISDNAFENXXXXXXXXXDRNKLKNIPT-QLFNNLKCLI 164 F L +L+ L L +N I I + F N+L P Q+ N + L+ Sbjct: 859 GFEFERLSHLRELYLQNNLIGFIGNLTFLPLRSLEILRLSGNRLVTFPVWQVTLNAR-LV 917 Query: 165 FLDLSNN 171 L L +N Sbjct: 918 ELSLGSN 924 Score = 36.7 bits (81), Expect = 0.002 Identities = 24/74 (32%), Positives = 34/74 (45%) Query: 3 DNHVEYLPGGIFESLVNLKTLRLQRNYLEEIDSEAFTSTKKLFHVDLSNNYLYTLPEKLF 62 DN + L G FE L +L+ L LQ N + I + F + L + LS N L T P Sbjct: 851 DNRIRELKGFEFERLSHLRELYLQNNLIGFIGNLTFLPLRSLEILRLSGNRLVTFPVWQV 910 Query: 63 ANNTFLETIDISNN 76 N L + + +N Sbjct: 911 TLNARLVELSLGSN 924 >DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. Length = 828 Score = 26.6 bits (56), Expect = 2.1 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Query: 2036 IQSIDKLKDATDESDDDEF-GENKKNRRK 2063 IQ K K+ TD+ DDDE EN K++++ Sbjct: 75 IQQAGKPKEETDDKDDDESDNENIKSQKE 103 >AJ517411-1|CAD56944.1| 1770|Apis mellifera vitellogenin precursor protein. Length = 1770 Score = 26.2 bits (55), Expect = 2.8 Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 5/75 (6%) Query: 59 EKLFANNTFLETIDISNNRVVYVPSDSFLGLYSLQILDLSKNKIQNIQNGTFSLKNLQIL 118 +K + L+ +DIS + VPSD+ + LYS + LS+ ++ + KN Sbjct: 1325 QKAVVLSMLLDEVDIS----MEVPSDALIALYSQGLFSLSEIDNLDVSLDVSNPKNAGKK 1380 Query: 119 KLSDN-KINNISDNA 132 K+ K+N D A Sbjct: 1381 KIDVRAKLNEYLDKA 1395 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.317 0.134 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 557,477 Number of Sequences: 429 Number of extensions: 24369 Number of successful extensions: 111 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 63 Number of HSP's gapped (non-prelim): 23 length of query: 2095 length of database: 140,377 effective HSP length: 69 effective length of query: 2026 effective length of database: 110,776 effective search space: 224432176 effective search space used: 224432176 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits) S2: 51 (24.6 bits)
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