Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000386-TA|BGIBMGA000386-PA|IPR008147|Glutamine
synthetase, beta-Grasp
(134 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 95 1e-20
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 95 2e-20
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 90 4e-19
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 88 2e-18
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 87 4e-18
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 86 7e-18
At1g27280.1 68414.m03323 paired amphipathic helix repeat-contain... 29 1.1
At2g42310.1 68415.m05237 expressed protein 28 2.5
At4g25020.1 68417.m03589 KOW domain-containing protein / D111/G-... 27 4.3
At1g29170.1 68414.m03569 expressed protein ; expression supporte... 27 5.7
At5g07210.1 68418.m00821 two-component responsive regulator fami... 26 7.5
At4g14370.1 68417.m02214 disease resistance protein (TIR-NBS-LRR... 26 7.5
At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21) ... 26 9.9
At3g57785.1 68416.m06437 expressed protein 26 9.9
At1g32850.1 68414.m04048 ubiquitin carboxyl-terminal hydrolase f... 26 9.9
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 95.5 bits (227), Expect = 1e-20
Identities = 41/85 (48%), Positives = 57/85 (67%)
Query: 18 DLEVPCDAILASYVWLDGTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDT 77
DL D I+A Y+W+ G+G+++RSK RT D LP W +DGS+T QA D+S+
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEV 70
Query: 78 FIFPEVIYHDPFRRGNHILVLADTY 102
I+P+ I+ DPFRRGN+ILV+ D Y
Sbjct: 71 IIYPQAIFKDPFRRGNNILVMCDAY 95
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 94.7 bits (225), Expect = 2e-20
Identities = 40/101 (39%), Positives = 63/101 (62%)
Query: 24 DAILASYVWLDGTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDTFIFPEV 83
D I+A Y+W+ G+GI+LRSK RT + +D +LP W +DGS+T QA ++S+ ++P+
Sbjct: 75 DRIIAEYIWIGGSGIDLRSKSRTIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQA 134
Query: 84 IYHDPFRRGNHILVLADTYQFNYQPTSKQMSCTPGDFLKNR 124
I+ DPFR GN+ILV+ DT+ +P + N+
Sbjct: 135 IFRDPFRGGNNILVICDTWTPAGEPIPTNKRAKAAEIFSNK 175
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 90.2 bits (214), Expect = 4e-19
Identities = 37/79 (46%), Positives = 55/79 (69%)
Query: 24 DAILASYVWLDGTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDTFIFPEV 83
D I+A Y+W+ G+G+++RSK RT D LP W +DGS+T QA ++S+ ++P+
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVILYPQA 76
Query: 84 IYHDPFRRGNHILVLADTY 102
I+ DPFRRGN+ILV+ D Y
Sbjct: 77 IFKDPFRRGNNILVMCDAY 95
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 88.2 bits (209), Expect = 2e-18
Identities = 37/85 (43%), Positives = 56/85 (65%)
Query: 18 DLEVPCDAILASYVWLDGTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDT 77
D+ + I+A Y+W+ G+G+++RSK RT D LP W +DGS+T QA +S+
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEV 70
Query: 78 FIFPEVIYHDPFRRGNHILVLADTY 102
++P+ I+ DPFRRGN+ILV+ D Y
Sbjct: 71 ILYPQAIFKDPFRRGNNILVMCDAY 95
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 87.0 bits (206), Expect = 4e-18
Identities = 35/77 (45%), Positives = 54/77 (70%)
Query: 26 ILASYVWLDGTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDTFIFPEVIY 85
I+A Y+W+ G+G+++RSK RT D LP W +DGS+T QA ++S+ ++P+ I+
Sbjct: 19 IIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAIF 78
Query: 86 HDPFRRGNHILVLADTY 102
DPFR+GN+ILV+ D Y
Sbjct: 79 KDPFRKGNNILVMCDAY 95
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 86.2 bits (204), Expect = 7e-18
Identities = 35/78 (44%), Positives = 55/78 (70%)
Query: 26 ILASYVWLDGTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDTFIFPEVIY 85
I+A Y+W+ G+G+++RSK RT + LP W +DGS+T QA D+S+ ++P+ I+
Sbjct: 19 IIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVILYPQAIF 78
Query: 86 HDPFRRGNHILVLADTYQ 103
DPFR+GN+ILV+ D Y+
Sbjct: 79 KDPFRKGNNILVMCDAYR 96
>At1g27280.1 68414.m03323 paired amphipathic helix repeat-containing
protein similar to transcription co-repressor Sin3
[Xenopus laevis] GI:4960210; contains Pfam profile
PF02671: Paired amphipathic helix repeat
Length = 225
Score = 29.1 bits (62), Expect = 1.1
Identities = 13/44 (29%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Query: 82 EVIYHDPFRRGNHILVLADTYQFNYQPTSKQMSCTPGDFLKNRM 125
E +YHD + NHIL L Y FN++ +++ G+ +++ +
Sbjct: 2 EEVYHDEPEKFNHILHLIRDY-FNHRDDRARITACMGELMRDHL 44
>At2g42310.1 68415.m05237 expressed protein
Length = 114
Score = 27.9 bits (59), Expect = 2.5
Identities = 16/43 (37%), Positives = 26/43 (60%), Gaps = 4/43 (9%)
Query: 1 MNSTPIALNKAAMRKYEDLEVPCDAILASYVWLDGTGINLRSK 43
MN TP A ++ RK+ED E+PC + S++ + G+ L +K
Sbjct: 46 MNRTPPAPGQS--RKWEDWELPC--YITSFLTIVILGVGLNAK 84
>At4g25020.1 68417.m03589 KOW domain-containing protein /
D111/G-patch domain-containing protein contains Pfam
profiles PF01585: G-patch domain, PF00467: KOW motif
Length = 375
Score = 27.1 bits (57), Expect = 4.3
Identities = 10/21 (47%), Positives = 15/21 (71%)
Query: 24 DAILASYVWLDGTGINLRSKD 44
+A+LA Y W G GI L++K+
Sbjct: 144 EALLAGYGWKPGQGIGLKAKE 164
>At1g29170.1 68414.m03569 expressed protein ; expression supported
by MPSS
Length = 1016
Score = 26.6 bits (56), Expect = 5.7
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Query: 22 PCDAILASYVWLD-GTGINLRSKDRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDT 77
P D + AS + L G N + +F +P+ LP Y D +++P SDT
Sbjct: 745 PADTLQASRLKLKFSDGDNTYNTFSSFQLLPETGTSLPDSYSDDDTFCRSSPYMSDT 801
>At5g07210.1 68418.m00821 two-component responsive regulator family
protein / response regulator family protein contains
Pfam profile: PF00072 response regulator receiver domain
Length = 621
Score = 26.2 bits (55), Expect = 7.5
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Query: 53 DHKDLPIWYFDGSNTAQANPDNSDTFIFPEVIYHDPFRRGNH 94
+H+D W F N Q+N + S+T PE + P NH
Sbjct: 545 NHEDDGDWTFVNINQGQSNGETSNTIASPET--NTPILNINH 584
>At4g14370.1 68417.m02214 disease resistance protein (TIR-NBS-LRR
class), putative similar to zinc finger protein
(GI:15811367) [Arabidopsis thaliana]; similar to
TIR-NBS-LRR (GI:27466164) [Arabidopsis thaliana]; similar
to disease resistance protein RPP1-WsB (GI:3860165)
[Arabidopsis thaliana]
Length = 1996
Score = 26.2 bits (55), Expect = 7.5
Identities = 13/49 (26%), Positives = 21/49 (42%)
Query: 44 DRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDTFIFPEVIYHDPFRRG 92
DR+ D I KD + +W+ + + N + PE+ D F G
Sbjct: 1055 DRSLDLICKDKAETEVWFAGLKSLIRQNRNKQAKSEIPEIHDSDCFSTG 1103
>At5g46740.1 68418.m05758 ubiquitin-specific protease 21 (UBP21)
identical to ubiquitin-specific protease 21 GI:11993482
[Arabidopsis thaliana]
Length = 732
Score = 25.8 bits (54), Expect = 9.9
Identities = 16/83 (19%), Positives = 36/83 (43%), Gaps = 8/83 (9%)
Query: 44 DRTFDFIPKDHKDLPIWYFDGSNTAQANPDNSDTFIFPEVIYHDPFRRGNHILVLADTYQ 103
D+T +F P++++D+ N +++ SD + P NH+ + +
Sbjct: 68 DKTLNFNPEENRDV--------NPDESSSSPSDKTLIAPPAQISPVSNNNHLRITNTSDS 119
Query: 104 FNYQPTSKQMSCTPGDFLKNRMK 126
+ Y+P + + D N+M+
Sbjct: 120 YLYRPPRRYIEYESDDDELNKME 142
>At3g57785.1 68416.m06437 expressed protein
Length = 114
Score = 25.8 bits (54), Expect = 9.9
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Query: 1 MNSTPIALNKAAMRKYEDLEVPCDAILASYVWLDGTGINLRSK 43
MN TP ++ RK+ED E+PC + S++ + G+ L +K
Sbjct: 46 MNRTPPPPGQS--RKWEDWELPC--YITSFLTIVILGVGLNAK 84
>At1g32850.1 68414.m04048 ubiquitin carboxyl-terminal hydrolase
family protein similar to ubiquitin-specific protease
UBP5 [Arabidopsis thaliana] GI:6648604; contains Pfam
profile PF00443: Ubiquitin carboxyl-terminal hydrolase
Length = 892
Score = 25.8 bits (54), Expect = 9.9
Identities = 16/55 (29%), Positives = 20/55 (36%)
Query: 72 PDNSDTFIFPEVIYHDPFRRGNHILVLADTYQFNYQPTSKQMSCTPGDFLKNRMK 126
PD S I + R + +L D Y FN QP PG K +K
Sbjct: 645 PDESSRSILSRDTETEDNDRELSLSLLRDYYSFNLQPLESDSVVNPGSVTKVLVK 699
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.139 0.437
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,474,285
Number of Sequences: 28952
Number of extensions: 147476
Number of successful extensions: 225
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 216
Number of HSP's gapped (non-prelim): 15
length of query: 134
length of database: 12,070,560
effective HSP length: 74
effective length of query: 60
effective length of database: 9,928,112
effective search space: 595686720
effective search space used: 595686720
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 54 (25.8 bits)
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