Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000375-TA|BGIBMGA000375-PA|IPR001269|Dihydrouridine
synthase, DuS
         (222 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom...   170   2e-43
SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo...   100   2e-22
SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2 |Schizosacc...    73   4e-14
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha...    42   7e-05
SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|c...    29   0.72 
SPAC11D3.02c |||ELLA family acetyltransferase |Schizosaccharomyc...    27   1.7  
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha...    27   1.7  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    25   8.9  

>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 326

 Score =  170 bits (413), Expect = 2e-43
 Identities = 85/206 (41%), Positives = 127/206 (61%), Gaps = 2/206 (0%)

Query: 10  KFADAKINKTYLKVCAPMVRYSKVHFRTLVRNFNADLCFTPMILADSFCQNSKARANEFM 69
           K  +    K  + + APMVRYSK+ FR LVR++N D+ +TPMILA  F  + K R  +F 
Sbjct: 10  KIFEINKGKRPVHIAAPMVRYSKLPFRQLVRDYNTDIVYTPMILAKEFL-HPKGRYFDFS 68

Query: 70  TTTTDFPVIAQFAANNRDDFVDASKLVYPYVDGVDLNCGCPQKWAMKDGYGCSLLSKPEI 129
           T   D  +I QF  ++      A++LV PYVDG+ +NCGCPQ WA+++G G +LL +PE 
Sbjct: 69  TNDADASLILQFGVDDPVILEKAAQLVGPYVDGIGINCGCPQTWAIQEGIGSALLDEPEK 128

Query: 130 IYDIVKGLRNVLPNKFSISVKIRLLNDIKKTVILCQQLEKCGVTFLT-SMEEHCTKSSGP 188
           ++ +V+ +++ L   F   VKIR+  D+ KT  L Q +EK G   +T        +SS P
Sbjct: 129 VHKLVRAVKSTLGESFCTEVKIRIAKDLNKTRHLMQVIEKSGADIITVHGRTRQDRSSFP 188

Query: 189 VDINGLKEVREALSVPMVANGGVKHL 214
           V+++ ++EVR  + +P+VANG VK L
Sbjct: 189 VNLDAIREVRPCVQIPVVANGDVKSL 214


>SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 399

 Score =  100 bits (239), Expect = 2e-22
 Identities = 65/208 (31%), Positives = 102/208 (49%), Gaps = 9/208 (4%)

Query: 22  KVCAPMVRYSKVHFRTLVRNFNADLCFTPMILADSFCQNSKARANEFMTTTT--DFPVIA 79
           ++ APMV  S++ +R L R   ADLC++PM  +  F ++   R   F T T   + P+I 
Sbjct: 20  RILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTIPEERPLII 79

Query: 80  QFAANNRDDFVDASKLVYPYVDGVDLNCGCPQKWAMKDGYGCSLLSKPEIIYDIVKGLRN 139
           QF  N+ +  + A+K+  PY D VD+N GCPQ  A K  YG  L     +I  I+  L  
Sbjct: 80  QFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIESIITKLHT 139

Query: 140 VLPNKFSISVKIRLLNDIKKTVILCQQLEKCGVTFLT---SMEEHCTKSSGPVDINGLKE 196
            L     ++ KIR+  D +KT+   + + K G + L     + E     +G  D   ++ 
Sbjct: 140 EL--SIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIADWEQIQM 197

Query: 197 VREAL--SVPMVANGGVKHLNDATTYMK 222
           +R+ L     + ANG + H  D    +K
Sbjct: 198 LRKNLPSETVLFANGNILHAQDIDRCIK 225


>SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 479

 Score = 72.5 bits (170), Expect = 4e-14
 Identities = 69/224 (30%), Positives = 107/224 (47%), Gaps = 32/224 (14%)

Query: 22  KVC-APMVRYSKVHFRTLVRNFNADLCFTPMILADSFCQ--------NSKARANEFMTTT 72
           KVC APMVR  ++  R L   + A+L + P I+  +           N +    +F+   
Sbjct: 9   KVCLAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCIDFVKPP 68

Query: 73  TD---FPV--------IAQFAANNRDDFVDASKLVYPYVDGVDLNCGCPQKWAMKDGYGC 121
           ++   F V        I Q  + + +  V+A+KLV   V G+DLNCGCP+ +++  G G 
Sbjct: 69  SNKVLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVHAGMGA 128

Query: 122 SLLSKPEIIYDIVKGLRNVL--PNKFSISVKIRLLNDIKKTVILCQQLEKCGVTFLTSME 179
            LL   + +  I+  L N +  P K SIS KIRLL   + T+ L +++   GV  +T   
Sbjct: 129 GLLKNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERICDTGVRAIT--- 185

Query: 180 EHC----TKSSGPVDINGLKE---VREALSVPMVANGGVKHLND 216
            HC     +++ P D + L E   V     V ++ NG V   ND
Sbjct: 186 VHCRTTPMRNTEPADRSYLSEIVGVCRNKDVSILVNGDVLSYND 229


>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 617

 Score = 41.9 bits (94), Expect = 7e-05
 Identities = 20/56 (35%), Positives = 33/56 (58%), Gaps = 1/56 (1%)

Query: 100 VDGVDLNCGCPQKWAMKDGYGCSLLSKPEIIYDIVKGLRNVLPNKFSISVKIRLLN 155
           VD +DLNCGCP     + G G SLL  P  +   ++G+ + +  +  ++VK+R+ N
Sbjct: 331 VDFLDLNCGCPIDLVFRQGAGSSLLENPGRLLRNLQGM-DAVSGQIPVTVKLRMGN 385


>SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 334

 Score = 28.7 bits (61), Expect = 0.72
 Identities = 12/42 (28%), Positives = 25/42 (59%)

Query: 172 VTFLTSMEEHCTKSSGPVDINGLKEVREALSVPMVANGGVKH 213
           V  + ++E H +     ++ NG+K++   LS+P+VA+  + H
Sbjct: 166 VVRIAAIEVHYSMLFREIEYNGVKKLCHDLSIPLVAHSPLAH 207


>SPAC11D3.02c |||ELLA family acetyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 150

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 11/20 (55%), Positives = 12/20 (60%)

Query: 89  FVDASKLVYPYVDGVDLNCG 108
           FV   K  YP VD +DL CG
Sbjct: 30  FVVEQKCAYPEVDEIDLKCG 49


>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
           synthase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 541

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 17/67 (25%), Positives = 35/67 (52%), Gaps = 5/67 (7%)

Query: 154 LNDIKKTVILCQQLEKCG---VTFLTSME-EHCTKSSGPVDINGLKEVREALSVPMVANG 209
           + ++ K V LCQ+  + G   V FL      +C  +  P+ +  L++  + + VP+   G
Sbjct: 265 VRNLGKPVELCQRYFQEGADEVVFLNITSFRNCPMADAPM-LQVLEKAAQTVFVPLTVGG 323

Query: 210 GVKHLND 216
           G++ ++D
Sbjct: 324 GIRDVSD 330


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 25.0 bits (52), Expect = 8.9
 Identities = 11/30 (36%), Positives = 16/30 (53%)

Query: 161 VILCQQLEKCGVTFLTSMEEHCTKSSGPVD 190
           +I C Q    GV   ++ E H  K+ GP+D
Sbjct: 547 MIWCSQFLLAGVVCESNFELHLYKAKGPLD 576


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.323    0.136    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,013,001
Number of Sequences: 5004
Number of extensions: 40727
Number of successful extensions: 87
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 75
Number of HSP's gapped (non-prelim): 8
length of query: 222
length of database: 2,362,478
effective HSP length: 70
effective length of query: 152
effective length of database: 2,012,198
effective search space: 305854096
effective search space used: 305854096
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 52 (25.0 bits)