Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000375-TA|BGIBMGA000375-PA|IPR001269|Dihydrouridine
synthase, DuS
         (222 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g67220.1 68418.m08473 nitrogen regulation family protein simi...   123   8e-29
At3g49640.1 68416.m05425 nitrogen regulation family protein simi...    90   1e-18
At3g63510.1 68416.m07154 nitrogen regulation family protein cont...    57   1e-08
At5g47970.1 68418.m05926 nitrogen regulation family protein stro...    49   2e-06
At4g38890.1 68417.m05508 dihydrouridine synthase family protein ...    40   0.002
At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative ...    29   2.5  
At5g45050.2 68418.m05524 disease resistance protein-related simi...    27   7.7  
At5g45050.1 68418.m05523 disease resistance protein-related simi...    27   7.7  
At3g10320.1 68416.m01238 expressed protein contains Pfam domain,...    27   7.7  

>At5g67220.1 68418.m08473 nitrogen regulation family protein similar
           to unknown protein (gb|AAF51525.1) ; contains Pfam
           domain PF01207: Dihydrouridine synthase (Dus); similar
           to (SP:P45672) NIFR3-like protein (SP:P45672)
           [Azospirillum brasilense]
          Length = 423

 Score =  123 bits (297), Expect = 8e-29
 Identities = 67/203 (33%), Positives = 108/203 (53%), Gaps = 5/203 (2%)

Query: 23  VCAPMVRYSKVHFRTLVRNFNADLCFTPMILADSFCQNSKARANEFMTTTTDFPVIAQFA 82
           + APMV  S++ FR L + + A   +TPM+ +  F +  K R  EF T   D P+  QF 
Sbjct: 91  IVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCKEDRPLFVQFC 150

Query: 83  ANNRDDFVDASKLVYPYVDGVDLNCGCPQKWAMKDGYGCSLLSKPEIIYDIVKGLRNVLP 142
           AN+ D  ++A+K V PY D VD+N GCPQ+ A +  YG  L+    ++  +V+ L   L 
Sbjct: 151 ANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGAFLMDNLPLVKSLVEKLAQNL- 209

Query: 143 NKFSISVKIRLLNDIKKTVILCQQLEKCGVTFLTSMEEHCTKSSGP---VDINGLKEVRE 199
               +S KIR+  +++ T+   + LE  G + L        +  G     D + +KEV+ 
Sbjct: 210 -NVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRDEKDGKKFRADWSAIKEVKN 268

Query: 200 ALSVPMVANGGVKHLNDATTYMK 222
           A+ +P++ANG V+ + D    +K
Sbjct: 269 AMRIPVLANGNVRCIEDVDNCIK 291


>At3g49640.1 68416.m05425 nitrogen regulation family protein similar
           to NITROGEN REGULATION PROTEIN NIFR3 (SP:Q08111)
           [Rhodobacter capsulatus]; contains Pfam domain PF01207:
           Dihydrouridine synthase (Dus)
          Length = 519

 Score = 90.2 bits (214), Expect = 1e-18
 Identities = 66/211 (31%), Positives = 107/211 (50%), Gaps = 19/211 (9%)

Query: 23  VCAPMVRYSKVHFRTLVRNFNADLCFTPMILADSF--CQ---NSKARANEFMTTTTD--- 74
           V APMVR   + FR L   + AD+ +   I+      C+   N  +  +EF+   TD   
Sbjct: 224 VLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDNVV 283

Query: 75  --------FPVIAQFAANNRDDFVDASKLVYPYVDGVDLNCGCPQKWAMKDGYGCSLLSK 126
                     V+ Q   ++    + AS++V   V  +D+N GCP+ ++++ G G +LLSK
Sbjct: 284 FSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAALLSK 343

Query: 127 PEIIYDIVKGLRNVLPNKFSISVKIRLLNDIKKTVILCQQLEKCGVTFL-TSMEEHCTKS 185
           PE+I+DI+  L+  L     ++ KIRLL     TV L +++EK GV  L     +   + 
Sbjct: 344 PELIHDILATLKRNL--DVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHGRKIADRP 401

Query: 186 SGPVDINGLKEVREALSVPMVANGGVKHLND 216
             P   + + +V  ALS+P++ANG V   +D
Sbjct: 402 RDPAKWDEIADVVAALSIPVIANGDVLEYDD 432


>At3g63510.1 68416.m07154 nitrogen regulation family protein
           contains Pfam domain PF01207: Dihydrouridine synthase
           (Dus); similar to (SP:P45672) NIFR3-like protein
           (SP:P45672) [Azospirillum brasilense]
          Length = 404

 Score = 56.8 bits (131), Expect = 1e-08
 Identities = 37/133 (27%), Positives = 68/133 (51%), Gaps = 7/133 (5%)

Query: 25  APMVRYSKVHFRTLVRNFNADL-CFTPMILADSFCQNSKARANEFMT-TTTDFPVIAQFA 82
           APM+ ++  H+RTL R        +T MI A++   + +   + F+  +    P++ Q  
Sbjct: 42  APMMDWTDNHYRTLARLITKHAWLYTEMIAAETLV-HQQTNLDRFLAFSPQQHPIVLQLG 100

Query: 83  ANNRDDFVDASKLVYPY-VDGVDLNCGCPQ-KWAMKDGYGCSLLSKPEIIYDIVKGLRNV 140
            +N ++   A+KL   Y  D ++LNCGCP  K A    +G SL+ KP+++ + +  +   
Sbjct: 101 GSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSAI--A 158

Query: 141 LPNKFSISVKIRL 153
                 ++VK R+
Sbjct: 159 ANTNVPVTVKCRI 171


>At5g47970.1 68418.m05926 nitrogen regulation family protein strong
           similarity to unknown protein (emb|CAB87804.1) ;
           contains Pfam domain PF01207: Dihydrouridine synthase
           (Dus); similar to (SP:P45672) NIFR3-like protein
           (SP:P45672) [Azospirillum brasilense]
          Length = 387

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 29/110 (26%), Positives = 54/110 (49%), Gaps = 5/110 (4%)

Query: 25  APMVRYSKVHFRTLVRNFNADL-CFTPMILADSFCQNSKARANEFMTTTTD-FPVIAQFA 82
           APM+ ++  H+RTL R        +T M+ A++         + F+  + D  P++ Q  
Sbjct: 15  APMMGWTDNHYRTLARLITKHAWLYTEMLAAETIVYQED-NLDSFLAFSPDQHPIVLQIG 73

Query: 83  ANNRDDFVDASKLVYPYV-DGVDLNCGCPQ-KWAMKDGYGCSLLSKPEII 130
             N ++   A++L   Y  D ++ NCGCP  K + +  +G  L+  P+ +
Sbjct: 74  GRNLENLAKATRLANAYAYDEINFNCGCPSPKVSGRGCFGALLMLDPKFV 123


>At4g38890.1 68417.m05508 dihydrouridine synthase family protein
           contains Pfam domain, PF01207: Dihydrouridine synthase
           (Dus)
          Length = 700

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 35/162 (21%), Positives = 65/162 (40%), Gaps = 5/162 (3%)

Query: 17  NKTYLKVCAPMVRYSKVHFRTLVRNFNADLCFTPMILADSFCQNSKARANEFMTTTTDFP 76
           +K YL   AP+     + FR L +   AD+    M +  +  Q   +        +++  
Sbjct: 347 DKLYL---APLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDL 403

Query: 77  VIAQFAANNRDDFVDASKLVYPY--VDGVDLNCGCPQKWAMKDGYGCSLLSKPEIIYDIV 134
              Q   +  D      +L+     VD +D+N GCP    +    G +LL+KP  + +IV
Sbjct: 404 FGVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIV 463

Query: 135 KGLRNVLPNKFSISVKIRLLNDIKKTVILCQQLEKCGVTFLT 176
           +   +++    +I V+        +   L   +   G T +T
Sbjct: 464 EVSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVT 505


>At1g22940.1 68414.m02867 thiamin biosynthesis protein, putative
           strong similarity to hydroxymethylpyrimidine
           kinase/thiamin-phosphate pyrophosphorylase (BTH1)
           GI:7488455 from [Brassica napus]
          Length = 522

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 12/23 (52%), Positives = 18/23 (78%)

Query: 189 VDINGLKEVREALSVPMVANGGV 211
           + ++GLKEV EA  +P+VA GG+
Sbjct: 451 IGLDGLKEVCEASKLPVVAIGGI 473


>At5g45050.2 68418.m05524 disease resistance protein-related similar
           to NL27 [Solanum tuberosum] GI:3947735; contains Pfam
           profiles PF03106: WRKY DNA -binding domain, PF00931:
           NB-ARC domain, PF00560: Leucine Rich Repeat
          Length = 1344

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 20/72 (27%), Positives = 28/72 (38%), Gaps = 5/72 (6%)

Query: 53  LADSFCQNSKARANEFMTTTTDFPVIAQFAANNRDDFVDASKLVYP-----YVDGVDLNC 107
           LA SFC  S A  N  +        + +   + R+  V  + LV       Y D  D   
Sbjct: 845 LAFSFCAPSHANQNSKLDLQPGSSSMTRLDPSWRNTLVGFAMLVQVAFSEGYCDDTDFGI 904

Query: 108 GCPQKWAMKDGY 119
            C  KW  K+G+
Sbjct: 905 SCVCKWKNKEGH 916


>At5g45050.1 68418.m05523 disease resistance protein-related similar
           to NL27 [Solanum tuberosum] GI:3947735; contains Pfam
           profiles PF03106: WRKY DNA -binding domain, PF00931:
           NB-ARC domain, PF00560: Leucine Rich Repeat
          Length = 1372

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 20/72 (27%), Positives = 28/72 (38%), Gaps = 5/72 (6%)

Query: 53  LADSFCQNSKARANEFMTTTTDFPVIAQFAANNRDDFVDASKLVYP-----YVDGVDLNC 107
           LA SFC  S A  N  +        + +   + R+  V  + LV       Y D  D   
Sbjct: 873 LAFSFCAPSHANQNSKLDLQPGSSSMTRLDPSWRNTLVGFAMLVQVAFSEGYCDDTDFGI 932

Query: 108 GCPQKWAMKDGY 119
            C  KW  K+G+
Sbjct: 933 SCVCKWKNKEGH 944


>At3g10320.1 68416.m01238 expressed protein contains Pfam domain,
           PF04577: Protein of unknown function (DUF563)
          Length = 494

 Score = 27.5 bits (58), Expect = 7.7
 Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 11/48 (22%)

Query: 90  VDASKLVYPYVDGVDLNCGCPQKWAMKDGYGCSLLSKPEIIYDIVKGL 137
           VD SK ++PYV  V  +CG            C+  SKP++IY ++  L
Sbjct: 13  VDESKKLFPYVFRVKTSCG-----------NCAKRSKPKLIYLLIFSL 49


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.323    0.136    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,140,898
Number of Sequences: 28952
Number of extensions: 202882
Number of successful extensions: 373
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 363
Number of HSP's gapped (non-prelim): 9
length of query: 222
length of database: 12,070,560
effective HSP length: 78
effective length of query: 144
effective length of database: 9,812,304
effective search space: 1412971776
effective search space used: 1412971776
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 58 (27.5 bits)