BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000368-TA|BGIBMGA000368-PA|IPR001128|Cytochrome P450, IPR002403|E-class P450, group IV (517 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 58 3e-09 SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 48 4e-06 SPAC26H5.12 |rpo41||mitochondrial DNA-directed RNA polymerase|Sc... 29 1.6 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 28 2.7 SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 27 4.8 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 27 6.3 SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual 27 6.3 SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 27 6.3 SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 27 6.3 SPAC10F6.14c |||ABC1 kinase family protein|Schizosaccharomyces p... 27 8.3 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 27 8.3 SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 8.3 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 58.0 bits (134), Expect = 3e-09 Identities = 41/184 (22%), Positives = 86/184 (46%), Gaps = 4/184 (2%) Query: 293 SKNDKSLLKTFLQQPQLDHKDIMGMMVDILMAAIDTTAYTTSFVLYHIARNKRCQDEMFE 352 S+N + K + + ++I + L A+ D T+ +++ +A + ++ E Sbjct: 288 SENKEGAEKPSVLIREFSDEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVRE 347 Query: 353 E-LHTLLPKKDDEITADVLSKASYVRSSIKESLRLNPVSIGIGRWLQKDI-VLKGYSIPK 410 E L D ++ D++ K +Y R+ +KE LRL P + + ++K + Y++PK Sbjct: 348 EQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPITPDYTVPK 407 Query: 411 GTVIVTQNMTSSRLPQFIRDPLTFKPERWMRGSPQYETIHPFLSLPFGHGPRSCIARRLA 470 +++ + + +P TF P+RW ++ P + FG+GP C+ +R A Sbjct: 408 DAMVIPTLYGALHDSKVYPEPETFNPDRWAPNGLAEQS--PKNWMVFGNGPHVCLGQRYA 465 Query: 471 EQNI 474 ++ Sbjct: 466 VNHL 469 >SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 47.6 bits (108), Expect = 4e-06 Identities = 48/221 (21%), Positives = 98/221 (44%), Gaps = 18/221 (8%) Query: 306 QPQLDHKDIMGMMVDILMAAIDTTAYTTSFVLYHIARNKRCQDEMFEELHTLLPKKDDEI 365 +P +H +I GMM+ +LMA T+A T +VL + + ++EE ++ ++ E+ Sbjct: 271 RPLKEH-EIAGMMIALLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVV-GENLEL 328 Query: 366 TADVLSKASYVRSSIKESLRLNPVSIGIGRWLQKDIVLKGYS--IPKGTVIVTQNMTSSR 423 D + I+E+LRL+P R +++D+ + G IP ++ ++ Sbjct: 329 KFDQYKDMPLLNYVIQETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLTAT 388 Query: 424 LPQFIRDPLTFKPERWM------RGSPQYETIHPFLS-------LPFGHGPRSCIARRLA 470 ++ F P+RW + Q + + ++ LPFG G CI + A Sbjct: 389 EEEYFTHATDFDPKRWNDRVNEDENAEQIDYGYGLVTKGAASPYLPFGAGRHRCIGEQFA 448 Query: 471 EQNICIILMRLIREFEIQWAGEELGVK-TLLINKPNKPVSL 510 ++ I+ + + ++ G+ V + ++ P PV + Sbjct: 449 YMHLSTIISKFVHDYTWTLIGKVPNVDYSSMVALPLGPVKI 489 >SPAC26H5.12 |rpo41||mitochondrial DNA-directed RNA polymerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1120 Score = 29.1 bits (62), Expect = 1.6 Identities = 34/117 (29%), Positives = 56/117 (47%), Gaps = 7/117 (5%) Query: 192 SSKNDFLTFLNRLNLEIIGVVAFNERFNSFALSEQDPESRSSKTIAAAFGSNSGVMKLDK 251 SS + LT L ++ + + F + ++F L+ Q +S+ AA + L++ Sbjct: 222 SSLSSLLTESIDLPIDEVSL-EFGNQGDTFNLARQKLLEKSAILSAAEVWKSEHESVLNR 280 Query: 252 GFLW--KMFSTPLYKKLVN-SQIYLEKISTDILIRKINLFESDDSKNDKSLLKTFLQ 305 G L K S+ Y V Q++ E+IS LI + L ES D KND+ + FL+ Sbjct: 281 GNLQVPKNVSSLFYSWYVQLEQLFKEEIS---LIDDLALNESLDKKNDRLIYGPFLK 334 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 28.3 bits (60), Expect = 2.7 Identities = 14/26 (53%), Positives = 17/26 (65%) Query: 218 FNSFALSEQDPESRSSKTIAAAFGSN 243 F S LS +P+S+S K I AA GSN Sbjct: 330 FKSLRLSYLEPKSQSEKGIRAATGSN 355 >SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 27.5 bits (58), Expect = 4.8 Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Query: 82 LNWRRYGSLVREKPIVNLVHVYDPDDIEA 110 LN G ++R++P VNL H+Y PD + A Sbjct: 317 LNQETRGLVLRQRP-VNLHHLYYPDSLPA 344 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 27.1 bits (57), Expect = 6.3 Identities = 14/40 (35%), Positives = 21/40 (52%) Query: 273 LEKISTDILIRKINLFESDDSKNDKSLLKTFLQQPQLDHK 312 ++K + L RK+ L E + NDK L +T + Q D K Sbjct: 76 IQKTEAEQLSRKVELLEEELETNDKLLRETTEKMRQTDVK 115 >SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual Length = 656 Score = 27.1 bits (57), Expect = 6.3 Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 2/39 (5%) Query: 399 KDIVLKGYSIPKGTVIVTQNMTSSRLPQ--FIRDPLTFK 435 ++I GYS+P G VI++ + T + L + +R P FK Sbjct: 443 RNIEQNGYSLPDGPVILSPDRTMAALHREVILRKPEVFK 481 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 27.1 bits (57), Expect = 6.3 Identities = 10/22 (45%), Positives = 12/22 (54%) Query: 425 PQFIRDPLTFKPERWMRGSPQY 446 P+ I DP KPE W+ P Y Sbjct: 275 PRMIPDPDAVKPEDWLEDEPLY 296 >SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 27.1 bits (57), Expect = 6.3 Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Query: 278 TDILIRKINLFESDDSKNDKSLLKTFLQQPQLDHKDIMGMMVDILMAAIDTTAYTTS 334 T L + + E+ K+D S L ++ + I G+M+ I ++A+D T TT+ Sbjct: 58 TSSLKSRFSTHEASSEKDDSSDFTLVLPSNRM-YIVIPGLMLSIFLSALDQTVITTA 113 >SPAC10F6.14c |||ABC1 kinase family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 535 Score = 26.6 bits (56), Expect = 8.3 Identities = 24/84 (28%), Positives = 42/84 (50%), Gaps = 3/84 (3%) Query: 209 IGVVAFNERFNSFALSEQDPESRSSKTIAAAFGSNSGVMKLDK-GFLWKMFSTPLYKKLV 267 IGV A++ +N+ AL+ ++ IAA + N K DK L + + L+K + Sbjct: 59 IGVYAWDRVYNAHALTRSIRTVYTASIIAADYKLNFSEKKADKIDALHQRVAQRLFKTIY 118 Query: 268 -NSQIYLEKISTDILIRKINLFES 290 N +Y+ K+ I ++ NL E+ Sbjct: 119 KNGGLYI-KMGQIIAMQSNNLPEA 141 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 26.6 bits (56), Expect = 8.3 Identities = 18/69 (26%), Positives = 33/69 (47%), Gaps = 4/69 (5%) Query: 131 RTNKPNVYNTGGLLATNGPDWWRLRSIF-QKNFTS---PQSVKTHVSDTDNIAKEFVEWI 186 R++ NV + +L T RS F Q ++ P S++ H + + + E++E Sbjct: 2324 RSSWENVISNVSILRTKFQTTKHKRSPFCQVTYSKVDIPWSMELHAASVEKVLNEYLELQ 2383 Query: 187 KRDKVSSKN 195 +RD + KN Sbjct: 2384 RRDLLQGKN 2392 >SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 26.6 bits (56), Expect = 8.3 Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 284 KINLFESDDSKNDKSLLKTFLQQPQLDHKDIMGMMVDILMAAIDTTAYTTS 334 + + E KND L +L + I G+M+ I +AA+D T TT+ Sbjct: 64 RFSTHEISSEKNDSPDFTLVLPSNRL-YIVIPGLMLSIFLAALDQTVITTA 113 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.320 0.136 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,539,686 Number of Sequences: 5004 Number of extensions: 113900 Number of successful extensions: 291 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 281 Number of HSP's gapped (non-prelim): 15 length of query: 517 length of database: 2,362,478 effective HSP length: 76 effective length of query: 441 effective length of database: 1,982,174 effective search space: 874138734 effective search space used: 874138734 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 56 (26.6 bits)
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