Silkworm BLAST Result against bee

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000368-TA|BGIBMGA000368-PA|IPR001128|Cytochrome P450,
IPR002403|E-class P450, group IV
         (517 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450 monoo...   206   5e-55
DQ232888-1|ABB36783.1|  499|Apis mellifera cytochrome P450 monoo...    79   9e-17
DQ244075-1|ABB36785.1|  548|Apis mellifera cytochrome P450 monoo...    77   5e-16
DQ067178-1|AAZ20250.1|  448|Apis mellifera conserved ATPase doma...    28   0.16 
AY601637-1|AAT11850.1|  683|Apis mellifera hexamerin 70b protein.      24   3.5  
AB264313-1|BAF43600.1|  900|Apis mellifera ecdysone-induced prot...    23   4.6  

>DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 517

 Score =  206 bits (502), Expect = 5e-55
 Identities = 150/498 (30%), Positives = 238/498 (47%), Gaps = 20/498 (4%)

Query: 27  FVGSEHASKVNEQGDNLMNFEDIPGPRSYPIIGTLHKYLPLIGDYDAEAL-DKNAILNWR 85
           +  + H +    +G        +PGP S PI GT   +   IG Y    + D    LN +
Sbjct: 32  WTATSHEASAPAEG-KFKTVSKVPGPFSLPIFGTRWIF-SCIGYYKLNKIHDAYKDLN-Q 88

Query: 86  RYGSLVREKPIVN--LVHVYDPDDIEAVFRQDHRYPARRSHTAMNYYRTNKPNVYNTGGL 143
           RYG+L +E+ + N  ++ V+   DIE + R++ RYP R     +++YR  + + Y   GL
Sbjct: 89  RYGALCKEEALWNFPMISVFSRQDIETIIRRNSRYPLRPPQEVISHYRRTRRDRYTNLGL 148

Query: 144 LATNGPDWWRLRSIFQKNFTSPQSVKTHVSDTDNIAKEFVEWIKRDKVSSK-NDFLTFLN 202
           +   G  W  LR       T+  +V       + +A  F+E I+R +V  K   F     
Sbjct: 149 VNEQGQTWHDLRVALTSELTAASTVLGFFPALNIVADSFIELIRRQRVGYKVTGFEELAY 208

Query: 203 RLNLEIIGVVAFNERFNSFAL-SEQDPESRSSKTIAAAF-GSNSGVMKLDKGFLWKMFST 260
           ++ LE    +            S  +  +R ++ +   F  S      L    LWK+  T
Sbjct: 209 KMGLESTCTLILGRHLGFLKPDSSSELATRLAEAVRIHFTASRDAFYGLP---LWKLLPT 265

Query: 261 PLYKKLVNSQIYLEKISTDILIRKINLFESDDSKND--KSLLKTFLQQPQLDHKDIMGMM 318
             YK+L+ S+  +  I ++I+   I   + DD+K++  +++ ++ L+Q  LD +D    +
Sbjct: 266 CAYKQLIESEDAIYNIISEIIETTIQE-KRDDAKDESVEAIFQSILRQKNLDIRDKKAAI 324

Query: 319 VDILMAAIDTTAYTTSFVLYHIARNKRCQDEMFEELHTLLPKKDDEITADVLSKASYVRS 378
           VD + A I T   T  F+   I RN   Q++++EE + L P   D +T D L KA Y+R+
Sbjct: 325 VDFIAAGIHTLGNTLVFLFDLIGRNPTVQNKLYEETYALAPAGCD-LTIDNLRKAKYLRA 383

Query: 379 SIKESLRLNPVSIGIGRWLQKDIVLKGYSIPKGTVIVTQNMTSSRLPQFIRDPLTFKPER 438
            I ESLRL P +  I R L + I L GY +  GTV++     +    +  +D   + PER
Sbjct: 384 CITESLRLIPTTTCIARILDEPIELSGYRLTAGTVVLLHTWIAGLNEENFKDAKKYLPER 443

Query: 439 WMRGSPQYETIHPFLSLPFGHGPRSCIARRLAEQNICIILMRLIREFEIQWAGEELGVKT 498
           W   +  +    P L  PFG G R C  +R  +  + +IL ++IREFEI    EEL ++ 
Sbjct: 444 WTTPTTPHS---PLLVAPFGAGRRICPGKRFVDLALQLILAKIIREFEI-IVEEELDLQF 499

Query: 499 LLINKPNKPVSLNFIPRS 516
             I  P  PVSL F  RS
Sbjct: 500 EFILAPKGPVSLGFRDRS 517


>DQ232888-1|ABB36783.1|  499|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 499

 Score = 79.0 bits (186), Expect = 9e-17
 Identities = 80/310 (25%), Positives = 134/310 (43%), Gaps = 14/310 (4%)

Query: 189 DKVSSKNDFLT---FLNRLNLEIIGVVAFNERFNSFALSEQDPESRSSKTIAAAFGSNSG 245
           DK+  KN+ +       R   ++IG  AF    +S   +E     R  + + A    N  
Sbjct: 163 DKLIEKNEPIECRELTARFTTDVIGSCAFGIDMSSMT-NENSEFRRMGREVFAVNFMNVM 221

Query: 246 VMKLDKGFLWKMFSTPLYKKLVNSQI-YLEKISTD-ILIRKINLFESDDSKNDKSLLKTF 303
            MKL K F+ +++    Y     +   +  ++ TD I  R  N     D  N    L+  
Sbjct: 222 RMKL-KQFMPRLYDLLGYVMPDRTFAPFFTRVVTDTIKYRNDNNIVRPDFINMLMELQKN 280

Query: 304 ---LQQPQLDHKDIMGMMVDILMAAIDTTAYTTSFVLYHIARNKRCQDEMFEELHTLLPK 360
              L+  +L    I        +A  +T++ T S  LY +A N+  Q ++ EE++T  PK
Sbjct: 281 PQKLENIKLTDSLIAAQAFVFFLAGFETSSTTMSNALYELALNQDVQKKLREEINTFCPK 340

Query: 361 KDDEITADVLSKASYVRSSIKESLRLNPVSIGIGRWLQKDIVLKG--YSIPKGTVIVTQN 418
            + E+  D + +  Y+    KE+LR+ P +  + R    D        +IPK   I    
Sbjct: 341 NNKELKYDDIKEMEYLDKVFKETLRMYPPASILMRKAISDYTFNDTKITIPKEMKIWIPA 400

Query: 419 MTSSRLPQFIRDPLTFKPERWMRGSPQYETIHPFLSLPFGHGPRSCIARRLAEQNICIIL 478
               R      +P +F PER+ + +    + HP   LPFG GPR+CI  R A     + L
Sbjct: 401 FAIHRDSAIYPNPDSFDPERFDQDA--MASRHPMHYLPFGDGPRNCIGARFAVYQTKVGL 458

Query: 479 MRLIREFEIQ 488
           + ++R  +++
Sbjct: 459 ITILRNHKVE 468


>DQ244075-1|ABB36785.1|  548|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 548

 Score = 76.6 bits (180), Expect = 5e-16
 Identities = 48/182 (26%), Positives = 85/182 (46%), Gaps = 4/182 (2%)

Query: 309 LDHKDIMGMMVDILMAAIDTTAYTTSFVLYHIARNKRCQDEMFEELHTLLPKKDDEITAD 368
           L  K++   +  I+    DTTA  +SF L  +  +   Q+++ +EL  +    D   T  
Sbjct: 333 LTDKEVKEQVDTIMFEGHDTTASGSSFFLAVMGCHPDIQEKVIQELDEIFGDSDRPATFQ 392

Query: 369 VLSKASYVRSSIKESLRLNPVSIGIGRWLQKDIVLKG--YSIPKGTVIVTQNMTSSRLPQ 426
              +  Y+   + E+LR+ P    I R ++ D+ L    Y+IP G  +V       R P 
Sbjct: 393 DTLEMKYLERCLLETLRMYPPVPLIAREIKTDLKLASGDYTIPAGCTVVIGTFKLHRQPH 452

Query: 427 FIRDPLTFKPERWMRGSPQYETIHPFLSLPFGHGPRSCIARRLAEQNICIILMRLIREFE 486
              +P  F P+ ++    +    H +  +PF  GPRSC+ R+ A   + I+L  ++R F 
Sbjct: 453 IYPNPDVFDPDNFL--PEKTANRHYYAFVPFSAGPRSCVGRKYAMLKLKIVLSTILRNFR 510

Query: 487 IQ 488
           ++
Sbjct: 511 VR 512



 Score = 29.1 bits (62), Expect = 0.093
 Identities = 39/164 (23%), Positives = 71/164 (43%), Gaps = 16/164 (9%)

Query: 47  EDIPGPRSYPIIGTLHKYLPLIGDYDAEALDKNAILNWRRYGSLVREKPIVNLV-HVYDP 105
           E IPGP + P+IG     L L G  D  A+    +     +  +V+      LV  + DP
Sbjct: 42  EKIPGPPALPLIGNA---LDLFGSPD--AMFSQVLKKAENFKDVVKIWVGPKLVICLIDP 96

Query: 106 DDIEAVFRQDHRYPARRSHTAMNYYRTNKPNVYNTGGLLATNGPDWWRLRSIFQKNFTSP 165
            D+E +    + Y  + +      YR  KP  +   GLL + G  W   R +    F   
Sbjct: 97  RDVEIIL-SSNVYIDKSTE-----YRFFKP--WLGDGLLISTGQKWRNHRKLIAPTF-HL 147

Query: 166 QSVKTHVSDTDNIAKEFVEWIKRDKVSSKNDFLTFLNRLNLEII 209
             +K+ +   +  A+  VE ++++    + D   +++ L ++I+
Sbjct: 148 NVLKSFIDLFNANARSVVEKMRKEN-GKEFDCHNYMSELTVDIL 190


>DQ067178-1|AAZ20250.1|  448|Apis mellifera conserved ATPase domain
           protein protein.
          Length = 448

 Score = 28.3 bits (60), Expect = 0.16
 Identities = 29/113 (25%), Positives = 56/113 (49%), Gaps = 7/113 (6%)

Query: 248 KLDKGFLWKMFSTPLYKKLVNSQIYLEKISTDILIRKINLFESDDSKNDKSLLKT--FLQ 305
           K+DK FL KM +   Y  ++ +  + +++S D+L+R   +  SD  K   S++K+     
Sbjct: 275 KIDKWFLHKMKNIIDYYLVLENTDHTKQLSHDVLLRAKQIGFSD--KQIASVVKSSELAV 332

Query: 306 QPQLDHKDIMGMMVDILMAAIDTTAYTTSFVLYHIARNKRCQDEMFEELHTLL 358
           + Q    +I  M+  I   A +  A TT+++  ++  N    D  F   +T++
Sbjct: 333 RIQRQENNIRPMVKQIDTVAAEWPA-TTNYL--YLTYNGTVHDVEFPGXYTMV 382


>AY601637-1|AAT11850.1|  683|Apis mellifera hexamerin 70b protein.
          Length = 683

 Score = 23.8 bits (49), Expect = 3.5
 Identities = 10/23 (43%), Positives = 14/23 (60%)

Query: 283 RKINLFESDDSKNDKSLLKTFLQ 305
           R  NL E+ D+ NDK  +  F+Q
Sbjct: 60  RTFNLVENLDNYNDKEAVNEFMQ 82


>AB264313-1|BAF43600.1|  900|Apis mellifera ecdysone-induced protein
           75 protein.
          Length = 900

 Score = 23.4 bits (48), Expect = 4.6
 Identities = 12/30 (40%), Positives = 16/30 (53%)

Query: 200 FLNRLNLEIIGVVAFNERFNSFALSEQDPE 229
           F  R +  I GVV F +R   F+L  QD +
Sbjct: 238 FSKRFSPAIRGVVEFAKRIPGFSLLAQDDQ 267


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.320    0.136    0.405 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 152,919
Number of Sequences: 429
Number of extensions: 6816
Number of successful extensions: 30
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 14
Number of HSP's gapped (non-prelim): 7
length of query: 517
length of database: 140,377
effective HSP length: 61
effective length of query: 456
effective length of database: 114,208
effective search space: 52078848
effective search space used: 52078848
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 46 (22.6 bits)