Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000364-TA|BGIBMGA000364-PA|IPR005654|AFG1-like ATPase
(342 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g30490.1 68417.m04329 AFG1-like ATPase family protein contain... 105 6e-23
At4g28070.1 68417.m04026 AFG1-like ATPase family protein contain... 99 4e-21
At2g25530.1 68415.m03056 AFG1-like ATPase family protein contain... 52 7e-07
At1g08820.1 68414.m00982 vesicle-associated membrane family prot... 38 0.007
At4g08320.1 68417.m01373 tetratricopeptide repeat (TPR)-containi... 30 1.9
At1g55550.1 68414.m06358 kinesin motor protein-related Similar t... 30 2.6
At5g06830.1 68418.m00772 expressed protein contains Pfam profile... 29 3.4
At5g40880.1 68418.m04964 WD-40 repeat family protein / zfwd3 pro... 29 5.9
At2g45660.1 68415.m05677 MADS-box protein (AGL20) 29 5.9
At5g24290.2 68418.m02858 integral membrane family protein contai... 28 7.9
At5g24290.1 68418.m02857 integral membrane family protein contai... 28 7.9
>At4g30490.1 68417.m04329 AFG1-like ATPase family protein contains
Pfam profile: PF03969 AFG1-like ATPase
Length = 497
Score = 105 bits (251), Expect = 6e-23
Identities = 66/201 (32%), Positives = 110/201 (54%), Gaps = 23/201 (11%)
Query: 30 GPWQAYTQKVSS-KALSKDPHQERVVQHLQKVYQEISN------YERPIIQEQNIGSFFN 82
GP Y +++S+ + ++ D Q ++ LQ++Y E+ + +R +++ S +
Sbjct: 89 GPLVEYERRISNGELMTGDICQISALRELQRLYDELVDSVDTCRLDRYNTSDKSSRSRWF 148
Query: 83 FFXXXXXXXXXXXXGVYIWGSVGGGKTMLMDLFYDTVPIK-EKLRVHFNSFMLNIHARIH 141
+ G+Y++G VG GKTMLMDLF+D +P +K R+HF+ FML++H+R+
Sbjct: 149 WSRLMPQTSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPCTWKKQRIHFHDFMLSVHSRLQ 208
Query: 142 ELKIKSGKGASSFRDEGSKPFDPIPPVAADITQESWLICFDEFQVTDIGDAMILKRLFTQ 201
+ KG S DP+ VA +I ++ L+C DEF VTD+ DA+IL RLF
Sbjct: 209 K-----HKGLS----------DPLEVVAQEIAHDAILLCLDEFMVTDVADALILNRLFGH 253
Query: 202 LFDNGNSQISPQNDPVNNLFK 222
LF NG ++ N + L++
Sbjct: 254 LFSNGVILVATSNRNPDKLYE 274
Score = 31.5 bits (68), Expect = 0.84
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Query: 233 RPRIINI-FGRNVKFAKSCGGVLDSTFEELCDRVVIAAD 270
RP+++ + GR ++ G FEELCDR + AAD
Sbjct: 340 RPQVVEVVMGRKLQIPLGANGCAYFPFEELCDRPLGAAD 378
>At4g28070.1 68417.m04026 AFG1-like ATPase family protein contains
Pfam profile: PF03969 AFG1-like ATPase
Length = 464
Score = 99.1 bits (236), Expect = 4e-21
Identities = 64/201 (31%), Positives = 104/201 (51%), Gaps = 23/201 (11%)
Query: 30 GPWQAYTQK-VSSKALSKDPHQERVVQHLQKVYQEI------SNYERPIIQEQNIGSFFN 82
GP Y ++ V+ + L D Q ++ LQ++Y E+ +R ++ S +
Sbjct: 61 GPLVEYERRIVAGELLDGDLCQLGTLRELQRLYDELVQSADACRLDRYSASAKSTRSNWF 120
Query: 83 FFXXXXXXXXXXXXGVYIWGSVGGGKTMLMDLFYDTVPIKEKL-RVHFNSFMLNIHARIH 141
+ G+Y++G VG GKTMLMDLF+ +P + R+HF++FML++H+R+
Sbjct: 121 WNKFVSHSSVSPVKGLYLYGGVGTGKTMLMDLFFHQLPASWRTQRIHFHNFMLSVHSRLQ 180
Query: 142 ELKIKSGKGASSFRDEGSKPFDPIPPVAADITQESWLICFDEFQVTDIGDAMILKRLFTQ 201
+ KG DP+ V +I E+ L+C DEF V D+ DA+IL RLF
Sbjct: 181 K-----HKGLE----------DPLEVVGLEIADEAILLCLDEFMVNDVADALILNRLFRH 225
Query: 202 LFDNGNSQISPQNDPVNNLFK 222
LF+NG ++ N +NL++
Sbjct: 226 LFNNGIILVATSNRAPDNLYE 246
Score = 29.5 bits (63), Expect = 3.4
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Query: 264 RVVIAADSEPKNLMKLDETEFGDADRALMDDLKITKDSEDAKATIFTGEEEMFACDRCLS 323
R++ A+ P L++ T DA + ++ S+D + E FA DR +S
Sbjct: 383 RLLCTAEGSPLELLERIVT-ISDAQQIAPRTSSRSRKSDDPDLCV--DNELGFAKDRTIS 439
Query: 324 RIMEMQTDEYWEK 336
R+ EM + EY E+
Sbjct: 440 RLTEMNSKEYLEQ 452
Score = 29.1 bits (62), Expect = 4.5
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Query: 234 PRIINI-FGRNVKFAKSCGGVLDSTFEELCDRVVIAAD 270
P+++ + GR ++ + G FEELCDR + AAD
Sbjct: 312 PQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAAD 349
>At2g25530.1 68415.m03056 AFG1-like ATPase family protein contains
Pfam profile: PF03969 AFG1-like ATPase
Length = 655
Score = 51.6 bits (118), Expect = 7e-07
Identities = 24/54 (44%), Positives = 37/54 (68%), Gaps = 2/54 (3%)
Query: 97 GVYIWGSVGGGKTMLMDLFYDTVP--IKEKLRVHFNSFMLNIHARIHELKIKSG 148
G+YI+G+VG GKTMLMD+FY I+ + R HF+ ML I+ ++H+ ++G
Sbjct: 243 GLYIYGNVGCGKTMLMDMFYGATDGIIRHRQRFHFHEAMLKINEQMHKYWKENG 296
Score = 41.1 bits (92), Expect = 0.001
Identities = 29/91 (31%), Positives = 43/91 (47%), Gaps = 5/91 (5%)
Query: 185 QVTDIGDAMILKRLFTQLFDNGNSQISPQNDPVNNLFKFLVSKETDTVRPRIIN-----I 239
++ IG + +R+ + + P ND V F+ + + TD I + +
Sbjct: 422 EIISIGSEVDYRRVAAKNSAENVHYLWPLNDAVLEEFEKMWRQVTDQYGGEITSATLPVM 481
Query: 240 FGRNVKFAKSCGGVLDSTFEELCDRVVIAAD 270
FGR V+ +SC GV TFE LC R V AAD
Sbjct: 482 FGRTVEVPESCSGVARFTFEYLCGRPVGAAD 512
Score = 35.9 bits (79), Expect = 0.039
Identities = 22/56 (39%), Positives = 32/56 (57%), Gaps = 1/56 (1%)
Query: 281 ETEFGDADRALMDDLKITK-DSEDAKATIFTGEEEMFACDRCLSRIMEMQTDEYWE 335
ETE R ++ + I+ S + ++ +GEEEMFA R SR++EMQT Y E
Sbjct: 587 ETEDSRLRRDVLAEGSISAAGSPSSIVSMLSGEEEMFAFARAASRLIEMQTPLYLE 642
Score = 31.9 bits (69), Expect = 0.64
Identities = 12/40 (30%), Positives = 23/40 (57%)
Query: 30 GPWQAYTQKVSSKALSKDPHQERVVQHLQKVYQEISNYER 69
GP Y++ V L DP+QE+VV + ++ + ++E+
Sbjct: 111 GPLTNYSKLVEQGRLQHDPYQEKVVSAFENLFGRLEHFEK 150
>At1g08820.1 68414.m00982 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 386
Score = 38.3 bits (85), Expect = 0.007
Identities = 41/172 (23%), Positives = 75/172 (43%), Gaps = 12/172 (6%)
Query: 166 PPVAADITQESWLICFDEFQVTDIGDAMILKRLFTQLFDNGNSQISPQNDPVNNLFKFLV 225
P +I +E ++ DE + D + ++ T F + ++ +
Sbjct: 164 PQYEGEIVKEPRMVGHDELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRM 223
Query: 226 SKET--DTVRPRIINIFGRNVKFAKSCGGVLDSTFEELCDRVV---IAADSEPK-----N 275
+KET D +R GR +K + + + D+ IA DSEPK +
Sbjct: 224 AKETGFDPIRSHKDADDGRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERD 283
Query: 276 LMKLDETEFGDADRALMDDLKITKDSEDAKATIFTGEEEMFACDRCLSRIME 327
+++L +T+ G R L D+LK+ KD E+ K + E ++ D +S++ME
Sbjct: 284 VVQLQKTD-GQNVRGL-DELKLVKDIEEMKLKVDALESKLKQADSTISKLME 333
>At4g08320.1 68417.m01373 tetratricopeptide repeat (TPR)-containing
protein glutamine-rich tetratricopeptide repeat (TPR)
containing protein (SGT) - Rattus
norvegicus,PID:e1285298 (SP|O70593); contains Pfam
profile PF00515 TPR Domain
Length = 426
Score = 30.3 bits (65), Expect = 1.9
Identities = 15/52 (28%), Positives = 27/52 (51%)
Query: 30 GPWQAYTQKVSSKALSKDPHQERVVQHLQKVYQEISNYERPIIQEQNIGSFF 81
G + +K KAL DPH E V ++++ Q+I ++ + QN +F+
Sbjct: 257 GKYAEAIEKGFKKALLLDPHNESVKENIRVAEQKIREEQQRQRRSQNTSTFY 308
>At1g55550.1 68414.m06358 kinesin motor protein-related Similar to
Kinesin proteins; Contains kinesin motor domain protein
motif and kinesin heavy chain signature motif
Length = 887
Score = 29.9 bits (64), Expect = 2.6
Identities = 29/108 (26%), Positives = 47/108 (43%), Gaps = 8/108 (7%)
Query: 98 VYIWGSVGGGKTMLMDLFYDT---VP--IKEKLR-VHFNSFMLNIHARIHELKIKSGKGA 151
++ +G G GKT M+ ++ VP IK + V ++ M IH + L+I G
Sbjct: 164 IFAYGQTGTGKTYTMEGLPNSPGIVPRAIKGLFKQVEESNHMFTIHFSM--LEIYMGNLK 221
Query: 152 SSFRDEGSKPFDPIPPVAADITQESWLICFDEFQVTDIGDAMILKRLF 199
E +KP PIPP + T + I + + D + RL+
Sbjct: 222 DLLLSEATKPISPIPPSLSIHTDPNGEIDIENLVKLKVDDFNEILRLY 269
>At5g06830.1 68418.m00772 expressed protein contains Pfam profile:
PF05600 protein of unknown function (DUF773)
Length = 549
Score = 29.5 bits (63), Expect = 3.4
Identities = 13/35 (37%), Positives = 23/35 (65%)
Query: 171 DITQESWLICFDEFQVTDIGDAMILKRLFTQLFDN 205
D+++ SW + + QV +IGD+ +L+ TQL D+
Sbjct: 329 DVSEISWDVSVETPQVEEIGDSALLESNQTQLTDS 363
>At5g40880.1 68418.m04964 WD-40 repeat family protein / zfwd3
protein (ZFWD3) contains 5 WD-40 repeats (PF00400);
contains Zinc finger C-x8-C-x5-C-x3-H type domain
(PF00642); identical to zfwd3 protein (GP:12057168)
{Arabidopsis thaliana}
Length = 472
Score = 28.7 bits (61), Expect = 5.9
Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 6/51 (11%)
Query: 10 CN--KCMRLLSSQTHAQHFVNDGPWQAYTQKVSSKAL----SKDPHQERVV 54
CN +C+R ++ Q A +++GPW + KA SKD H E VV
Sbjct: 216 CNSGQCVRSINLQAEAGSLISEGPWVFLGLPNAVKAFNVQNSKDVHLEGVV 266
>At2g45660.1 68415.m05677 MADS-box protein (AGL20)
Length = 214
Score = 28.7 bits (61), Expect = 5.9
Identities = 26/77 (33%), Positives = 31/77 (40%), Gaps = 4/77 (5%)
Query: 231 TVRPRIINIFGRNVKFAKSCGGVLDSTFE--ELCDRVVIAADSEPKNLMKLDETEFGDAD 288
T RI N R V F+K G+L FE LCD V PK KL E +
Sbjct: 6 TQMKRIENATSRQVTFSKRRNGLLKKAFELSVLCDAEVSLIIFSPKG--KLYEFASSNMQ 63
Query: 289 RALMDDLKITKDSEDAK 305
+ L+ TKD K
Sbjct: 64 DTIDRYLRHTKDRVSTK 80
>At5g24290.2 68418.m02858 integral membrane family protein contains
Pfam domain PF01988: Integral membrane protein
Length = 534
Score = 28.3 bits (60), Expect = 7.9
Identities = 23/105 (21%), Positives = 45/105 (42%), Gaps = 6/105 (5%)
Query: 207 NSQISPQNDPVNNLFKFLVSKET-DTVRPRIINIFGRNVKFAKSCGGVLDSTFEELCDRV 265
N + S ++ +NL L ++E + P RNV K G ++ +L +
Sbjct: 170 NERSSSDSEEKSNLENLLATQENYELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDI 229
Query: 266 VIAADSEPKNLMKLDETEFGDADRALMDDLKITKDSEDAKATIFT 310
+ EP ++ +++ + + + +I D ED + TIFT
Sbjct: 230 PVVEPDEPSDIEEMESPV-----KVYVPETRIEDDQEDKEGTIFT 269
>At5g24290.1 68418.m02857 integral membrane family protein contains
Pfam domain PF01988: Integral membrane protein
Length = 550
Score = 28.3 bits (60), Expect = 7.9
Identities = 23/105 (21%), Positives = 45/105 (42%), Gaps = 6/105 (5%)
Query: 207 NSQISPQNDPVNNLFKFLVSKET-DTVRPRIINIFGRNVKFAKSCGGVLDSTFEELCDRV 265
N + S ++ +NL L ++E + P RNV K G ++ +L +
Sbjct: 186 NERSSSDSEEKSNLENLLATQENYELYCPSCSTCITRNVVLKKRKRGKHVNSSLDLKPDI 245
Query: 266 VIAADSEPKNLMKLDETEFGDADRALMDDLKITKDSEDAKATIFT 310
+ EP ++ +++ + + + +I D ED + TIFT
Sbjct: 246 PVVEPDEPSDIEEMESPV-----KVYVPETRIEDDQEDKEGTIFT 285
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.136 0.413
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,108,739
Number of Sequences: 28952
Number of extensions: 335471
Number of successful extensions: 892
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 873
Number of HSP's gapped (non-prelim): 19
length of query: 342
length of database: 12,070,560
effective HSP length: 82
effective length of query: 260
effective length of database: 9,696,496
effective search space: 2521088960
effective search space used: 2521088960
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 60 (28.3 bits)
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