GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000357-TA|BGIBMGA000357-PA|undefined
         (159 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g64580.1 68418.m08116 AAA-type ATPase family protein similar ...    28   3.4  
At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger) fa...    28   3.4  
At5g63890.2 68418.m08022 histidinol dehydrogenase, putative / HD...    27   4.5  
At5g63890.1 68418.m08021 histidinol dehydrogenase, putative / HD...    27   4.5  
At5g04860.1 68418.m00509 expressed protein                             27   5.9  
At3g48710.1 68416.m05319 expressed protein putative protein - Ar...    27   5.9  
At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model...    27   7.8  
At2g03610.1 68415.m00321 F-box family protein contains F-box dom...    27   7.8  
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID...    27   7.8  

>At5g64580.1 68418.m08116 AAA-type ATPase family protein similar to
           zinc dependent protease [Arabidopsis thaliana]
           GI:7650138; contains Pfam profile PF00004: ATPase AAA
           family
          Length = 855

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 21/57 (36%), Positives = 29/57 (50%), Gaps = 7/57 (12%)

Query: 102 YICKESLYSITILKRR----ETKSQESLEIPNELKYRWVAESHPVSV-ACLCTRDYQ 153
           YI +E L  +  LKR+    ET  ++S E+P ELK R       V+V AC     Y+
Sbjct: 549 YIGREEL--LEALKRQKGTFETGQEDSTEVPEELKLRLAYREAAVAVLACYLPDQYR 603


>At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 383

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 26/96 (27%), Positives = 44/96 (45%), Gaps = 12/96 (12%)

Query: 46  RNDVILLDNSVETRTRKR--GNIQVENKEIEDLGENSVPRFI-----ETRNCNKTQQPTC 98
           R D I L+   E R +K+    +   NKE+E+LG  SV   I     E +NC    +  C
Sbjct: 274 RTDDIKLELDDERREKKKLEEELMELNKELEELGSESVEAAIVRLQEEVKNCKNILK--C 331

Query: 99  RPPYICKESLYSITILKRRETKSQESLEIPNELKYR 134
               +C +    + I+K      Q+ ++   E+++R
Sbjct: 332 G---VCFDRPKEVVIVKCYHLFCQQCIQRSLEIRHR 364


>At5g63890.2 68418.m08022 histidinol dehydrogenase, putative / HDH,
           putative strong similarity to SP|P24226 Histidinol
           dehydrogenase, chloroplast precursor (EC 1.1.1.23) (HDH)
           {Brassica oleracea var.capitata}; contains Pfam profile
           PF00815: histidinol dehydrogenase
          Length = 466

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 13/30 (43%), Positives = 20/30 (66%), Gaps = 1/30 (3%)

Query: 53  DNSVETRTRKRGNIQVENKEIEDLGENSVP 82
           DN+V+  T +   +Q+ NK +ED+ E SVP
Sbjct: 75  DNAVKEYTERFDKVQL-NKVVEDMSELSVP 103


>At5g63890.1 68418.m08021 histidinol dehydrogenase, putative /
          HDH, putative strong similarity to SP|P24226 Histidinol
          dehydrogenase, chloroplast precursor (EC 1.1.1.23)
          (HDH) {Brassica oleracea var.capitata}; contains Pfam
          profile PF00815: histidinol dehydrogenase
          Length = 452

 Score = 27.5 bits (58), Expect = 4.5
 Identities = 13/30 (43%), Positives = 20/30 (66%), Gaps = 1/30 (3%)

Query: 53 DNSVETRTRKRGNIQVENKEIEDLGENSVP 82
          DN+V+  T +   +Q+ NK +ED+ E SVP
Sbjct: 61 DNAVKEYTERFDKVQL-NKVVEDMSELSVP 89


>At5g04860.1 68418.m00509 expressed protein 
          Length = 782

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 20/81 (24%), Positives = 35/81 (43%)

Query: 7   DVLGDKENVRPNPYYTEPFDPDTSPEELSALIVDYANMIRNDVILLDNSVETRTRKRGNI 66
           D LG++     N  Y   FD D   + L ++I D    + N      N  E   R + + 
Sbjct: 614 DTLGERLYEGCNQAYVLKFDKDAEIKRLPSVIKDNKADMGNQKQGGKNKSEQPERSKESE 673

Query: 67  QVENKEIEDLGENSVPRFIET 87
           + E +E+   G+ S   +I++
Sbjct: 674 EQEEEEVVCRGKESCREYIKS 694


>At3g48710.1 68416.m05319 expressed protein putative protein -
           Arabidopsis thaliana, EMBL:AL078465.1
          Length = 462

 Score = 27.1 bits (57), Expect = 5.9
 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 7/68 (10%)

Query: 32  EELSALIVDY--ANMIRNDVILLDNSVETRTRKRGNIQVENKEIEDLGENSVPRFIETRN 89
           EEL+  ++++        D++L D+  ET+ RK+      +K +   GE+S       R 
Sbjct: 186 EELAVRVLEFLVCPKATRDILLADSEKETKKRKKST----SKNVTS-GESSHVPAKRRRQ 240

Query: 90  CNKTQQPT 97
             K +QPT
Sbjct: 241 AKKQEQPT 248


>At4g16340.1 68417.m02476 adapter protein SPIKE1 (SPK1) One model
          reflects the alignment of a full-length cDNA sequence
          gi:18496702.  There are multiple frame shifts in the
          gene model resulting in a truncated protein.  The
          alternate model includes modifications in exons 14, 17
          and 29 to compensate for frame shifts and maximize the
          protein length.  It is not based on EST data.  adapter
          protein SPIKE1 [Arabidopsis thaliana] GI:18496703
          Length = 1757

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%)

Query: 10 GDKENVRPNPYYTEPFD-PDTSPEELSALIVDYANMIRNDV 49
          G  EN+RP+ + T+ F+ PDT  E    L    +  I  DV
Sbjct: 56 GHYENIRPDSFQTQIFEGPDTDTETEIRLASARSATIEEDV 96


>At2g03610.1 68415.m00321 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 216

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 16/60 (26%), Positives = 28/60 (46%), Gaps = 5/60 (8%)

Query: 7   DVLGD--KENVRPNPYYTEPFDPDTSPEEL---SALIVDYANMIRNDVILLDNSVETRTR 61
           D  GD  KE    NPY   PF  D  P+E      +++  +  + N++++  + V  R +
Sbjct: 96  DFSGDSPKEEGLTNPYSNHPFRFDEKPQEYIWKRKIVIAESGEMMNEMLIFGHGVTMRAQ 155


>At1g67120.1 68414.m07636 midasin-related similar to Midasin
            (MIDAS-containing protein) (Swiss-Prot:Q12019)
            [Saccharomyces cerevisiae]; similar to Midasin
            (MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
            sapiens]; contains Prosite PS00017: ATP/GTP-binding site
            motif A (P-loop)
          Length = 5336

 Score = 26.6 bits (56), Expect = 7.8
 Identities = 20/78 (25%), Positives = 40/78 (51%), Gaps = 6/78 (7%)

Query: 23   EPFDPDTS--PEELSALIVDYANMIRNDVILLDNSVETRTRKRGNI--QVENKEIEDLGE 78
            EP + +TS  PEE +   V+  +   +D   L+  ++T+    G +   V+N++I+D  E
Sbjct: 4629 EPEESNTSDKPEEGNDENVEQDDF--DDTDNLEEKIQTKEEALGGLTPDVDNEQIDDDME 4686

Query: 79   NSVPRFIETRNCNKTQQP 96
                  +E  + N+ ++P
Sbjct: 4687 MDKTEEVEKEDANQQEEP 4704


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.314    0.132    0.389 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,101,680
Number of Sequences: 28952
Number of extensions: 162301
Number of successful extensions: 298
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 298
Number of HSP's gapped (non-prelim): 9
length of query: 159
length of database: 12,070,560
effective HSP length: 76
effective length of query: 83
effective length of database: 9,870,208
effective search space: 819227264
effective search space used: 819227264
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 56 (26.6 bits)