Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000355-TA|BGIBMGA000355-PA|IPR002853|Transcription
factor TFIIE, alpha subunit
         (421 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su...    58   3e-09
SPBC418.02 |||NatA N-acetyltransferase complex subunit |Schizosa...    28   2.1  
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote...    28   2.8  
SPBC31F10.16 |||ChAPs family protein|Schizosaccharomyces pombe|c...    28   2.8  
SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomy...    28   2.8  
SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb...    28   2.8  
SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schi...    27   3.7  
SPAC3H1.08c |||DUF1640 family protein|Schizosaccharomyces pombe|...    27   3.7  
SPAC29A4.19c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||...    27   3.7  
SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar...    27   4.9  
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc...    27   6.5  

>SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha
           subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 448

 Score = 57.6 bits (133), Expect = 3e-09
 Identities = 45/209 (21%), Positives = 92/209 (44%), Gaps = 22/209 (10%)

Query: 7   VTEVPSSLKQLARLVVRGFYTIEDALIVDMLVRNPCMKEDDICELLKFER---------- 56
           ++  P  +++L ++++R FY     + +D ++R+       I  +L F R          
Sbjct: 1   MSNAPEIVQRLIKMIMRAFYETRHIIFMDAILRHSAYVLHKIRTILTFGRLTDEQTALLM 60

Query: 57  ----KMLRARISILKNDKF--IQVRLKMETGLDGKAQKVNYYFINYKTFVNVVKYKLDLM 110
               K  R     L+ D+   IQ R +M+ G   +     Y++I++ + ++ +K+++  +
Sbjct: 61  GIPIKECRFIAGKLREDRLLAIQSRTEMKEGQQ-RQYHTTYFYIDFCSTIDSIKWRMHQL 119

Query: 111 RKRLETEERDATSRASFKCPACGKTFTDLEADQLYDMATQE--FQCTFCSAVVDEDMSAL 168
            K +E   R+      + CP C K F+ L+   +  + T E  F C  C   + +D  + 
Sbjct: 120 VKTVEDRMRNDFDSKGYVCPFCNKKFSSLD---VLSLVTNEGTFACNVCGTELKDDEESA 176

Query: 169 PKKDSRLLLAKFNEQLETLYILLREVEGI 197
               S+  L K   Q+  +   L+ V+ I
Sbjct: 177 EMMSSQKRLGKLMGQVNGIIDALKRVDEI 205


>SPBC418.02 |||NatA N-acetyltransferase complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 28.3 bits (60), Expect = 2.1
 Identities = 11/36 (30%), Positives = 20/36 (55%)

Query: 383 NGKTVPLTSVDDDVIAQMTPTEKETYIQIYQEYYSH 418
           N K + L  +++D I    P+E E  + I ++Y+ H
Sbjct: 591 NKKIMKLQKIEEDKIKSYIPSESEEPLVIDEDYFGH 626


>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
           Mug36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1646

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 18/62 (29%), Positives = 31/62 (50%), Gaps = 2/62 (3%)

Query: 147 MATQEFQCTFCSAVVDEDMSALPKKDSRLLLAKFN-EQLETLYILLREVEGIKLAPEILE 205
           ++  +  C+F +AVV   M    K   R L+ + N   +  L + L EV G+ L  E++ 
Sbjct: 60  LSNLQLNCSFLNAVVSLPMINFTKGTLRRLILRLNVTDIVNLNVEL-EVNGLSLEIELVP 118

Query: 206 PE 207
           P+
Sbjct: 119 PD 120


>SPBC31F10.16 |||ChAPs family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 679

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 13/38 (34%), Positives = 20/38 (52%)

Query: 233 GEATRNQGMLVEETRVDVTIGDDKPARDAGALRKERPV 270
           G A+ +Q  + EE+  +  +G DKP    G+L K   V
Sbjct: 472 GNASPDQNEVAEESVAEEVVGLDKPETSVGSLPKNATV 509


>SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 581

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 1/38 (2%)

Query: 196 GIKLAPEILEPE-PVDINTIRGLTSKQSALRPGGEQWS 232
           G+KL+ +I++   P+D+   R +TS  + L    E+WS
Sbjct: 106 GLKLSKKIVDASTPIDLKRKRAVTSLSTGLLSKREKWS 143


>SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 557

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 15/27 (55%), Positives = 17/27 (62%), Gaps = 2/27 (7%)

Query: 219 SKQSALRPGGEQWSGEATRNQGMLVEE 245
           SKQ  LR  G  WSG+A RNQ +L  E
Sbjct: 25  SKQ--LRHRGPDWSGKAIRNQTILCHE 49


>SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein
            Urb1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1568

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 16/63 (25%), Positives = 26/63 (41%)

Query: 274  ESTIASNEQSDSAHSTDXXXXXXXXXXXXXXXXGKEKNDDIMSVLLAHEKQPSAGNPVSN 333
            +ST+ S  +S   H T                  +  +   + ++LAHEK P AG   S+
Sbjct: 1139 KSTLNSFLESAIVHVTSFSNLRLCLTLAFTMTAEQCDSSRFLQMILAHEKNPLAGEDSSD 1198

Query: 334  AVK 336
             +K
Sbjct: 1199 EIK 1201


>SPAC3H1.08c |||DUF1640 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 211

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 21/80 (26%), Positives = 41/80 (51%), Gaps = 3/80 (3%)

Query: 11  PSSLKQLARLVVRGFYTIEDALIVDMLVRNPCMKEDDICELLK-FERKMLRARISILKND 69
           P  LKQ +R + +  Y++++A  +  L+R   +  + + EL K    K  +  +S  +  
Sbjct: 36  PELLKQSSRRLEQAGYSVKNAETITNLMRT--ITGEALTELEKNIGFKAKQESVSFQQKR 93

Query: 70  KFIQVRLKMETGLDGKAQKV 89
            F+Q+R  +ET  + +  KV
Sbjct: 94  TFLQIRKYLETIEENEFDKV 113


>SPAC29A4.19c |||P-type ATPase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1096

 Score = 27.5 bits (58), Expect = 3.7
 Identities = 9/20 (45%), Positives = 17/20 (85%)

Query: 396 VIAQMTPTEKETYIQIYQEY 415
           V+A+M+P +K TY+++ Q+Y
Sbjct: 810 VLARMSPFDKATYVELCQKY 829


>SPAC27F1.04c |nuf2||spindle pole body protein
           Nuf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 441

 Score = 27.1 bits (57), Expect = 4.9
 Identities = 25/96 (26%), Positives = 44/96 (45%), Gaps = 5/96 (5%)

Query: 33  IVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETGLDGKAQKVNYY 92
           I+   ++N C  +  I +LLK +R  L+  +S + N  F ++R +     D   QK    
Sbjct: 87  ILSQFMQNICFHDFTIQDLLKPDRNRLQLILSAVIN--FAKLREERLQQFDDDIQKRESL 144

Query: 93  FINYKTFVNVVKYKLDLMRKRLETEERDATSRASFK 128
               +T+  +   + DL  K L +++R   S A  K
Sbjct: 145 L---ETYTLLDAQRKDLEEKVLLSQDRKLESEAIIK 177


>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 822

 Score = 26.6 bits (56), Expect = 6.5
 Identities = 12/34 (35%), Positives = 20/34 (58%)

Query: 87  QKVNYYFINYKTFVNVVKYKLDLMRKRLETEERD 120
           QK++YY  NY TF+ V   +   + K+   +E+D
Sbjct: 495 QKLDYYSGNYDTFLKVRAERDVQLAKKARQQEKD 528


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.315    0.132    0.368 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,687,973
Number of Sequences: 5004
Number of extensions: 63661
Number of successful extensions: 174
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 166
Number of HSP's gapped (non-prelim): 12
length of query: 421
length of database: 2,362,478
effective HSP length: 75
effective length of query: 346
effective length of database: 1,987,178
effective search space: 687563588
effective search space used: 687563588
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 55 (26.2 bits)