Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000355-TA|BGIBMGA000355-PA|IPR002853|Transcription
factor TFIIE, alpha subunit
         (421 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g03280.1 68414.m00306 transcription initiation factor IIE (TF...    68   1e-11
At4g20340.1 68417.m02969 transcription initiation factor IIE (TF...    56   6e-08
At4g20810.1 68417.m03020 transcription initiation factor IIE (TF...    47   2e-05
At3g60300.1 68416.m06740 RWD domain-containing protein contains ...    33   0.47 
At3g21770.1 68416.m02746 peroxidase 30 (PER30) (P30) (PRXR9) ide...    31   1.9  
At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive eff...    29   4.4  
At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive eff...    29   4.4  
At1g12150.1 68414.m01407 expressed protein contains Pfam profile...    29   4.4  
At4g27910.1 68417.m04006 PHD finger protein-related / SET domain...    29   7.7  

>At1g03280.1 68414.m00306 transcription initiation factor IIE
           (TFIIE) alpha subunit family protein / general
           transcription factor TFIIE family protein contains Pfam
           profile: PF02002 TFIIE alpha subunit
          Length = 479

 Score = 67.7 bits (158), Expect = 1e-11
 Identities = 73/296 (24%), Positives = 128/296 (43%), Gaps = 52/296 (17%)

Query: 31  ALIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETG--------- 81
           A+++D L R   ++E+D+ + L+   K LR  I + + +K I    + ET          
Sbjct: 51  AVVLDALARRQWVREEDLAKDLQLHAKQLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAA 110

Query: 82  ----LDGKAQ-KV-----NYYFINYKTFVNVVKYKLDLMRKRLETEERDATSRASFKCPA 131
                DG+A+ KV     +Y  ++Y    +VV+++L  M+KRL+ E  D  +   + CP 
Sbjct: 111 VAATTDGRAEDKVKLHTHSYCCLDYAQICDVVRFRLHRMKKRLKDELEDKNTVQEYGCPN 170

Query: 132 CGKTFTDLEADQLYDMATQEFQCTFCSA-------------VVDEDMSALPKKDSRL--L 176
           C + +  L+A +L  M    F C  C+              VVD D +A  ++   L  +
Sbjct: 171 CQRKYNALDALRLISMVDDSFHCENCNGELVVECNKLTSEEVVDGDDNARRRRRENLKNM 230

Query: 177 LAKFNEQLETLYILLREVEGIKLAPEILEPEPVDINTIRGLTSKQSALRPGGEQWSGEAT 236
           L K   Q++ L   L  V+ +        P P   + +        A R  G+    +  
Sbjct: 231 LQKLEVQMKPLMDQLNRVKDL--------PIPEFGSFLAWEARAAMAARENGDLNPNDPL 282

Query: 237 RNQG-------MLVEETRVDVTIGD---DKPARDAGALRKERPVWMVESTIASNEQ 282
           R+QG         + ET+V+V +GD   D  ++   +  K  P WM++  +   E+
Sbjct: 283 RSQGGYGSTPMPFLGETKVEVNLGDGNEDVKSKGGDSSLKVLPPWMIKEGMNLTEE 338


>At4g20340.1 68417.m02969 transcription initiation factor IIE
           (TFIIE) alpha subunit family protein / general
           transcription factor TFIIE family protein contains Pfam
           profile: PF02002 TFIIE alpha subunit
          Length = 527

 Score = 55.6 bits (128), Expect = 6e-08
 Identities = 36/146 (24%), Positives = 67/146 (45%), Gaps = 19/146 (13%)

Query: 32  LIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFIQVRLKMETG---------- 81
           +++D L R   ++E+D+ + +K   K LR  I   +  KF+    + ET           
Sbjct: 52  IVLDALTRRQWVREEDLAKEVKRNAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAV 111

Query: 82  ---LDGKAQKV------NYYFINYKTFVNVVKYKLDLMRKRLETEERDATSRASFKCPAC 132
               DG+A+        +Y  ++Y    ++V+YKL  ++K+ + E  D  +   + CP C
Sbjct: 112 GGTTDGRAEDNVKFHTHSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCPNC 171

Query: 133 GKTFTDLEADQLYDMATQEFQCTFCS 158
            + +  L+A +L  M    F C  C+
Sbjct: 172 KRKYNALDALRLISMEDDSFHCENCN 197


>At4g20810.1 68417.m03020 transcription initiation factor IIE
           (TFIIE) alpha subunit family protein / general
           transcription factor TFIIE family protein contains Pfam
           profile: PF02002 TFIIE alpha subunit
          Length = 416

 Score = 47.2 bits (107), Expect = 2e-05
 Identities = 30/110 (27%), Positives = 56/110 (50%), Gaps = 9/110 (8%)

Query: 32  LIVDMLVRNPCMKEDDICELLKFERKMLRARISILKNDKFI-QVRLKMETGL--DGKAQ- 87
           L++D L R   ++E+D+ + LK   K LR  +   +  +FI +V  K ++    +G+ + 
Sbjct: 34  LVLDALTRRQWVREEDLAKELKLNTKQLRTILRYFEEQQFIMRVHRKEKSSATTNGRGED 93

Query: 88  --KVNYYF---INYKTFVNVVKYKLDLMRKRLETEERDATSRASFKCPAC 132
             KV+ Y    ++Y    +V++YKL  M+K  +    D  +   + CP C
Sbjct: 94  KVKVHMYSYCCLDYSQIYDVIRYKLHRMKKEFKDVLEDKDNVQEYGCPNC 143


>At3g60300.1 68416.m06740 RWD domain-containing protein contains
           weak similarity to RING finger protein 25 (RING finger
           protein AO7) (Swiss-Prot:Q9QZR0) [Mus musculus]
          Length = 366

 Score = 32.7 bits (71), Expect = 0.47
 Identities = 30/136 (22%), Positives = 55/136 (40%), Gaps = 8/136 (5%)

Query: 108 DLMRKRLETEERDATSRASFK----CPACGKTFTDLEADQLYDMATQEFQCTFCSAVVDE 163
           D +  R  +  R+  S ++ K    CP C K F   + + + D+   +      S +  +
Sbjct: 174 DTLHMRRGSTAREGNSGSADKSLGNCPVCRKIFHSSDIEHVLDLVGAQSSLQDSSLIPGK 233

Query: 164 DMSALPKKDS-RLLLAKFNEQLETLYI---LLREVEGIKLAPEILEPEPVDINTIRGLTS 219
           D   L + DS  +   +F   L+T      L++  + I + P +  P P   +T R    
Sbjct: 234 DQDPLLQSDSENIRRERFESILKTQEEKGGLVQPKKNISVVPGMYLPPPAHASTSRNEEE 293

Query: 220 KQSALRPGGEQWSGEA 235
           +    +  GE+   EA
Sbjct: 294 EAGESQEQGEEEPKEA 309


>At3g21770.1 68416.m02746 peroxidase 30 (PER30) (P30) (PRXR9)
           identical to SP|Q9LSY7 Peroxidase 30 precursor (EC
           1.11.1.7) (Atperox P30) (PRXR9) (ATP7a) {Arabidopsis
           thaliana}
          Length = 329

 Score = 30.7 bits (66), Expect = 1.9
 Identities = 17/53 (32%), Positives = 25/53 (47%)

Query: 194 VEGIKLAPEILEPEPVDINTIRGLTSKQSALRPGGEQWSGEATRNQGMLVEET 246
           VE IK   E + P+ V    I  LT++ + +  GG  WS    R  G +  +T
Sbjct: 105 VERIKALLEKVCPKTVSCADIIALTARDAVVATGGPSWSVPTGRRDGRISNKT 157


>At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 676

 Score = 29.5 bits (63), Expect = 4.4
 Identities = 17/88 (19%), Positives = 36/88 (40%)

Query: 4   ERYVTEVPSSLKQLARLVVRGFYTIEDALIVDMLVRNPCMKEDDICELLKFERKMLRARI 63
           E  V +  + LK+   +       +  A+++    + P M  +++CE  KF R +    +
Sbjct: 365 ELLVVDEAAQLKECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLV 424

Query: 64  SILKNDKFIQVRLKMETGLDGKAQKVNY 91
            +  N   + V+ +M   +     K  Y
Sbjct: 425 LLGHNKHLLDVQYRMHPSISRFPNKEFY 452


>At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 839

 Score = 29.5 bits (63), Expect = 4.4
 Identities = 17/88 (19%), Positives = 36/88 (40%)

Query: 4   ERYVTEVPSSLKQLARLVVRGFYTIEDALIVDMLVRNPCMKEDDICELLKFERKMLRARI 63
           E  V +  + LK+   +       +  A+++    + P M  +++CE  KF R +    +
Sbjct: 528 ELLVVDEAAQLKECESVAALQLPGLRHAILIGDEFQLPAMVHNEMCEKAKFGRSLFERLV 587

Query: 64  SILKNDKFIQVRLKMETGLDGKAQKVNY 91
            +  N   + V+ +M   +     K  Y
Sbjct: 588 LLGHNKHLLDVQYRMHPSISRFPNKEFY 615


>At1g12150.1 68414.m01407 expressed protein contains Pfam profile
           PF05701: Plant protein of unknown function (DUF827);
           expression supported by MPSS
          Length = 548

 Score = 29.5 bits (63), Expect = 4.4
 Identities = 28/112 (25%), Positives = 57/112 (50%), Gaps = 4/112 (3%)

Query: 97  KTFVNVVKYKL-DLMRKRLETEERDATSRASFKCPACGKTFTDLEADQLYDMATQEFQCT 155
           ++ VN ++ +L DL R+R E ++++A  R   +     +     E+ +L  M T+  +  
Sbjct: 325 RSLVNSLRMELEDLRREREELQQKEA-ERLEIEETKKLEALKQ-ESLKLEQMKTEAIEAR 382

Query: 156 FCSAVVDEDMSALPKKDSRLLLAKFNEQLETLYILLREVEGIKLAPEILEPE 207
             +A ++  + +L K+    ++A   E  + L +++REVE  K A E +  E
Sbjct: 383 NEAANMNRKIESLKKETEAAMIAA-EEAEKRLELVIREVEEAKSAEEKVREE 433


>At4g27910.1 68417.m04006 PHD finger protein-related / SET
           domain-containing protein (TX4) nearly identical over
           285 amino acids to trithorax 4 [Arabidopsis thaliana]
           GI:16118405; contains Pfam profiles PF00856: SET domain,
           PF00855: PWWP domain; identical to cDNA trithorax 4
           (TX4) partial cds GI:16118404
          Length = 1027

 Score = 28.7 bits (61), Expect = 7.7
 Identities = 21/78 (26%), Positives = 38/78 (48%), Gaps = 9/78 (11%)

Query: 128 KCPACGKTFTDLEADQ-----LYDMATQEFQCTFCSAVVDEDMSALPKKDSRLLLAKFNE 182
           +C  C K     E DQ     L D+   ++ C  C A  + D+S   K++S+  +AK + 
Sbjct: 418 RCDGC-KVRIHAECDQISDRHLKDLRETDYYCPTCRAKFNFDLSDSEKQNSKSKVAKGDG 476

Query: 183 QL---ETLYILLREVEGI 197
           Q+   + + ++   VEG+
Sbjct: 477 QMVLPDKVIVVCAGVEGV 494


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.315    0.132    0.368 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,576,569
Number of Sequences: 28952
Number of extensions: 325791
Number of successful extensions: 838
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 830
Number of HSP's gapped (non-prelim): 10
length of query: 421
length of database: 12,070,560
effective HSP length: 83
effective length of query: 338
effective length of database: 9,667,544
effective search space: 3267629872
effective search space used: 3267629872
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 61 (28.7 bits)