BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000343-TA|BGIBMGA000343-PA|IPR000618|Insect cuticle protein (118 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g18590.1 68417.m02752 expressed protein 29 0.82 At1g15280.2 68414.m01829 glycine-rich protein 28 1.4 At1g15280.1 68414.m01828 glycine-rich protein 28 1.4 At3g14890.2 68416.m01883 phosphoesterase identical to phosphoest... 27 3.3 At3g14890.1 68416.m01882 phosphoesterase identical to phosphoest... 27 3.3 At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 27 4.4 At5g25140.1 68418.m02979 cytochrome P450 family protein CYTOCHRO... 27 4.4 At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note:... 27 4.4 At5g61480.1 68418.m07714 leucine-rich repeat transmembrane prote... 26 5.8 At3g28455.1 68416.m03555 CLE25, putative CLAVATA3/ESR-Related 25... 26 5.8 At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 26 5.8 At3g33520.1 68416.m04291 actin-related protein 6 (ARP6) nearly i... 26 7.6 At3g07400.1 68416.m00882 lipase class 3 family protein contains ... 26 7.6 At1g74360.1 68414.m08615 leucine-rich repeat transmembrane prote... 26 7.6 At1g73370.1 68414.m08492 sucrose synthase, putative / sucrose-UD... 26 7.6 >At4g18590.1 68417.m02752 expressed protein Length = 106 Score = 29.1 bits (62), Expect = 0.82 Identities = 12/22 (54%), Positives = 15/22 (68%) Query: 53 RDEQGEVINVGTDDESIVIKGS 74 R + G VI TDD+ IV+KGS Sbjct: 31 RSDVGSVIGKSTDDQQIVVKGS 52 >At1g15280.2 68414.m01829 glycine-rich protein Length = 585 Score = 28.3 bits (60), Expect = 1.4 Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 1/42 (2%) Query: 23 DDNVQLLKFDSDNDGLGSYRFLYEQTDGS-KRDEQGEVINVG 63 D+ V +K+D+D DG SY E++ G D+ G V G Sbjct: 53 DEEVGTVKYDNDEDGEDSYEDDEEESGGGIDNDKSGVVKEAG 94 >At1g15280.1 68414.m01828 glycine-rich protein Length = 584 Score = 28.3 bits (60), Expect = 1.4 Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 1/42 (2%) Query: 23 DDNVQLLKFDSDNDGLGSYRFLYEQTDGS-KRDEQGEVINVG 63 D+ V +K+D+D DG SY E++ G D+ G V G Sbjct: 53 DEEVGTVKYDNDEDGEDSYEDDEEESGGGIDNDKSGVVKEAG 94 >At3g14890.2 68416.m01883 phosphoesterase identical to phosphoesterase [Arabidopsis thaliana] GI:21630064; contains Pfam profile PF00645: Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region Length = 638 Score = 27.1 bits (57), Expect = 3.3 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%) Query: 30 KFDSDNDGLGSYRFLYEQTDGSKRDEQGEVINVGTDDESI 69 K D DND + L E+T+ K E GE++ +DES+ Sbjct: 361 KMDEDNDDTEAKIKLTEETNKRKHSEVGEMVE---EDESL 397 >At3g14890.1 68416.m01882 phosphoesterase identical to phosphoesterase [Arabidopsis thaliana] GI:21630064; contains Pfam profile PF00645: Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region Length = 694 Score = 27.1 bits (57), Expect = 3.3 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%) Query: 30 KFDSDNDGLGSYRFLYEQTDGSKRDEQGEVINVGTDDESI 69 K D DND + L E+T+ K E GE++ +DES+ Sbjct: 417 KMDEDNDDTEAKIKLTEETNKRKHSEVGEMVE---EDESL 453 >At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to glutamine synthetase, chloroplast precursor (glutamate-- ammonia ligase, GS2) [Arabidopsis thaliana] SWISS-PROT:Q43127 Length = 430 Score = 26.6 bits (56), Expect = 4.4 Identities = 16/59 (27%), Positives = 27/59 (45%) Query: 25 NVQLLKFDSDNDGLGSYRFLYEQTDGSKRDEQGEVINVGTDDESIVIKGSYSWVAPDGI 83 +V L + N+ + +R L Q+D S + ++N+ T S I Y W+ GI Sbjct: 31 SVVLKQKKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGI 89 >At5g25140.1 68418.m02979 cytochrome P450 family protein CYTOCHROME P450 71B1 - Thlaspi arvense, EMBL:L24438 Length = 496 Score = 26.6 bits (56), Expect = 4.4 Identities = 15/37 (40%), Positives = 18/37 (48%), Gaps = 1/37 (2%) Query: 15 HSAPQGPKDDNVQLL-KFDSDNDGLGSYRFLYEQTDG 50 H G KDD V LL K + GLG ++F T G Sbjct: 253 HLEDGGSKDDIVDLLLKVERGEIGLGEFQFTRNHTKG 289 >At1g08135.1 68414.m00894 cation/hydrogen exchanger (CHX6b) Note: CHX6a and CHX6b were originally 1 gene but were split pased on alignments with other family members; may be a pseudogene and requires futher investigation; monovalent cation:proton antiporter family 2 (CPA2) member, PMID:11500563 Length = 824 Score = 26.6 bits (56), Expect = 4.4 Identities = 12/27 (44%), Positives = 17/27 (62%) Query: 12 ALAHSAPQGPKDDNVQLLKFDSDNDGL 38 AL + P GP +V + KFDS N+G+ Sbjct: 348 ALGLAVPNGPPLGSVFIQKFDSFNEGI 374 >At5g61480.1 68418.m07714 leucine-rich repeat transmembrane protein kinase, putative Length = 1041 Score = 26.2 bits (55), Expect = 5.8 Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 3/33 (9%) Query: 62 VGTDDESIVIKGSYSWVAPDGITYTVTYVADDK 94 + TD+ V+ GSY ++AP+ Y T D K Sbjct: 877 IQTDESMSVVAGSYGYIAPE---YAYTLQVDKK 906 >At3g28455.1 68416.m03555 CLE25, putative CLAVATA3/ESR-Related 25 (CLE25) Length = 81 Score = 26.2 bits (55), Expect = 5.8 Identities = 13/35 (37%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Query: 5 LVLFSFLA-LAHSAPQGPKDDNVQLLKFDSDNDGL 38 L++F + LA+SAP P +NV+ L+F + L Sbjct: 18 LIVFLLVGILANSAPSVPSSENVKTLRFSGKDVNL 52 >At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA topoisomerase II / DNA gyrase (TOP2) identical to SP|P30182 DNA topoisomerase II (EC 5.99.1.3) {Arabidopsis thaliana} Length = 1473 Score = 26.2 bits (55), Expect = 5.8 Identities = 12/35 (34%), Positives = 17/35 (48%) Query: 38 LGSYRFLYEQTDGSKRDEQGEVINVGTDDESIVIK 72 L Y F D SK E + I V DD+ +V++ Sbjct: 1307 LAQYNFGSAPADSSKTAETSKAIAVDDDDDDVVVE 1341 >At3g33520.1 68416.m04291 actin-related protein 6 (ARP6) nearly identical to actin-related protein 6 (ARP6) [Arabidopsis thaliana] GI:21427467; contains Pfam profile PF00022: Actin Length = 421 Score = 25.8 bits (54), Expect = 7.6 Identities = 12/23 (52%), Positives = 16/23 (69%), Gaps = 2/23 (8%) Query: 70 VIKGSYSWVAPDGITYTVTYVAD 92 +IK +Y V PDG+T+T YV D Sbjct: 238 LIKSTY--VLPDGVTHTKGYVKD 258 >At3g07400.1 68416.m00882 lipase class 3 family protein contains Pfam profile PF01764: Lipase Length = 1003 Score = 25.8 bits (54), Expect = 7.6 Identities = 12/31 (38%), Positives = 17/31 (54%) Query: 21 PKDDNVQLLKFDSDNDGLGSYRFLYEQTDGS 51 P D N +LLK D GL F++ Q +G+ Sbjct: 570 PGDKNAELLKVDITGFGLHLCSFVHAQVNGN 600 >At1g74360.1 68414.m08615 leucine-rich repeat transmembrane protein kinase, putative similar to brassinosteroid insensitive 1 GB:AAC49810 (putative receptor protein kinase); contains Pfam profiles: PF00560 Leucine Rich Repeat (17 repeats), PF00069 Eukaryotic protein kinase domain Length = 1106 Score = 25.8 bits (54), Expect = 7.6 Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Query: 59 VINVGTDDESIVIKGSYSWVAPD-GITYTVTYVAD 92 ++NVG S VI G+ +VAP+ G T+ T D Sbjct: 965 LLNVGDSHVSTVIAGTIGYVAPEYGQTWQATTRGD 999 >At1g73370.1 68414.m08492 sucrose synthase, putative / sucrose-UDP glucosyltransferase, putative similar to sucrose synthase GI:6682841 from [Citrus unshiu] Length = 942 Score = 25.8 bits (54), Expect = 7.6 Identities = 17/67 (25%), Positives = 33/67 (49%), Gaps = 6/67 (8%) Query: 45 YEQTDGSKRDEQGEVINVGTDDESIVIKGSYSWVAPDGITY--TVTY--VADDKG--FQP 98 ++ + + R+E+ E+ + E +KG + W+A Y + Y +AD KG QP Sbjct: 617 FDMSKSNDREEKAEIKKMHDLIEKYKLKGKFRWIAAQTDRYRNSELYRCIADTKGVFVQP 676 Query: 99 SIEQGPG 105 ++ + G Sbjct: 677 ALYEAFG 683 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.314 0.136 0.392 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,212,933 Number of Sequences: 28952 Number of extensions: 142919 Number of successful extensions: 235 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 227 Number of HSP's gapped (non-prelim): 15 length of query: 118 length of database: 12,070,560 effective HSP length: 72 effective length of query: 46 effective length of database: 9,986,016 effective search space: 459356736 effective search space used: 459356736 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.9 bits) S2: 54 (25.8 bits)
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