SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000333-TA|BGIBMGA000333-PA|IPR000618|Insect cuticle
protein
         (174 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g28080.1 68417.m04027 expressed protein                             32   0.19 
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr...    30   0.99 
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr...    30   0.99 
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ...    29   1.7  
At1g72110.1 68414.m08335 expressed protein                             29   2.3  
At1g26930.1 68414.m03283 kelch repeat-containing F-box family pr...    27   5.3  
At1g09070.1 68414.m01012 C2 domain-containing protein / src2-lik...    27   5.3  
At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein           27   7.0  
At4g39980.1 68417.m05662 2-dehydro-3-deoxyphosphoheptonate aldol...    27   7.0  
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex...    27   7.0  
At5g06340.1 68418.m00710 diadenosine 5',5'''-P1,P4-tetraphosphat...    27   9.2  
At3g53080.1 68416.m05850 galactose-binding lectin family protein...    27   9.2  

>At4g28080.1 68417.m04027 expressed protein
          Length = 1660

 Score = 32.3 bits (70), Expect = 0.19
 Identities = 26/63 (41%), Positives = 32/63 (50%), Gaps = 7/63 (11%)

Query: 92   NNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVN-YVADENGYQPTGNAIP 150
            +NG A Q  G PR +  NP A   +   S  W S  G P+S N Y+A  NG + T N  P
Sbjct: 1498 SNGEANQFNG-PRIM--NPHAAEFIP--SQPWVS-NGYPVSPNGYLASPNGAEITQNGYP 1551

Query: 151  TSP 153
             SP
Sbjct: 1552 LSP 1554


>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
           protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
           F-box domain
          Length = 451

 Score = 29.9 bits (64), Expect = 0.99
 Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)

Query: 63  KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 119
           K S   G E + FG EI     Y Y   TN   +  +  VPR L G+     + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243


>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
           protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
           F-box domain
          Length = 451

 Score = 29.9 bits (64), Expect = 0.99
 Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)

Query: 63  KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 119
           K S   G E + FG EI     Y Y   TN   +  +  VPR L G+     + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243


>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
            subunit (RPB205) (RPII) (RPB1) nearly identical to
            P|P18616 DNA-directed RNA polymerase II largest subunit
            (EC 2.7.7.6) {Arabidopsis thaliana}
          Length = 1840

 Score = 29.1 bits (62), Expect = 1.7
 Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)

Query: 91   TNNGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGNA 148
            T+ G +    G      G  P  P  +  S  +S TSP   P S +Y      Y PT  +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614

Query: 149  I-PTSP 153
              PTSP
Sbjct: 1615 YSPTSP 1620



 Score = 29.1 bits (62), Expect = 1.7
 Identities = 21/57 (36%), Positives = 26/57 (45%), Gaps = 4/57 (7%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSPPVPEQIAR---ALAYIAKNIPL 172
            S+S TSP   P S  Y      Y PT  +  PTSP    Q A+   ++AY   N  L
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARL 1747



 Score = 28.7 bits (61), Expect = 2.3
 Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 6/72 (8%)

Query: 83   GSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 142
            G   +   ++ G +    G      G  P  P      +S TSP   P S  Y     GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587

Query: 143  QPTGNAI-PTSP 153
             PT  A  PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599



 Score = 28.3 bits (60), Expect = 3.0
 Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S +Y      Y PT  A  PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648



 Score = 28.3 bits (60), Expect = 3.0
 Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S +Y      Y PT  A  PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655



 Score = 28.3 bits (60), Expect = 3.0
 Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S +Y      Y PT  A  PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704



 Score = 28.3 bits (60), Expect = 3.0
 Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S  Y     GY PT  +  PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718



 Score = 27.5 bits (58), Expect = 5.3
 Identities = 27/97 (27%), Positives = 39/97 (40%), Gaps = 6/97 (6%)

Query: 62   PKVSEGYGAETVKFGNEINPDGSYTY--FYETNNGIAAQEQGVPRNLGGNPPAVPVVA-- 117
            P  S GY   +  + +  +P  S T   +  T+ G +        +  G  P  P  +  
Sbjct: 1539 PSSSPGYSPSSPGY-SPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPT 1597

Query: 118  QGSFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
              S+S TSP   P S +Y      Y PT  +  PTSP
Sbjct: 1598 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1634



 Score = 27.1 bits (57), Expect = 7.0
 Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S +Y      Y PT  +  PTSP
Sbjct: 1607 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1641



 Score = 27.1 bits (57), Expect = 7.0
 Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S  Y      Y PT  A  PTSP
Sbjct: 1628 SYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSP 1662



 Score = 27.1 bits (57), Expect = 7.0
 Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S +Y      Y PT  +  PTSP
Sbjct: 1663 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1697



 Score = 26.6 bits (56), Expect = 9.2
 Identities = 15/35 (42%), Positives = 17/35 (48%), Gaps = 1/35 (2%)

Query: 120  SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 153
            S+S TSP   P S +Y      Y PT     PTSP
Sbjct: 1677 SYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSP 1711


>At1g72110.1 68414.m08335 expressed protein
          Length = 479

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 14/39 (35%), Positives = 21/39 (53%)

Query: 68  YGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNL 106
           +G+ T+ F N + PD   ++F      IAA   GVP+ L
Sbjct: 388 FGSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426


>At1g26930.1 68414.m03283 kelch repeat-containing F-box family
           protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
           F-box domain
          Length = 421

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 16/51 (31%), Positives = 24/51 (47%)

Query: 59  RVVPKVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGN 109
           R   K S   G + + FG E++    Y Y   TN+   A+   +PR L G+
Sbjct: 157 RYADKESLAVGTDLLVFGWEVSSYVIYRYSLLTNSWSTAKSMNMPRCLFGS 207


>At1g09070.1 68414.m01012 C2 domain-containing protein / src2-like
           protein, putative similar to cold-regulated gene SRC2
           [Glycine max] GI:2055230; contains Pfam profile PF00168:
           C2 domain; identical to cDNA  src2-like protein
           GI:3426059
          Length = 324

 Score = 27.5 bits (58), Expect = 5.3
 Identities = 17/55 (30%), Positives = 21/55 (38%), Gaps = 6/55 (10%)

Query: 98  QEQGVPRNLGGNPPAVPVVAQGSFSWTSPE------GVPISVNYVADENGYQPTG 146
           Q+ G P   GG PP      QG +    P+      G P    Y   + GY P G
Sbjct: 204 QQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPGYPPQGPYGYPQQGYPPQG 258


>At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein
          Length = 774

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 17/62 (27%), Positives = 28/62 (45%), Gaps = 5/62 (8%)

Query: 104 RNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGYQPTGNAIPTSPPVPEQIARAL 163
           RNL  N  AVP +  G + W S      +++ ++D       G A P +   P+ I  A 
Sbjct: 74  RNLVNNAAAVPRLGIGGYEWWSE-----ALHGISDVGPGAKFGGAFPGATSFPQVITTAA 128

Query: 164 AY 165
           ++
Sbjct: 129 SF 130


>At4g39980.1 68417.m05662 2-dehydro-3-deoxyphosphoheptonate aldolase
           1 / 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
           1 / DAHP synthetase 1 (DHS1) nearly identical to
           SP|P29965
          Length = 525

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 20/69 (28%), Positives = 30/69 (43%), Gaps = 1/69 (1%)

Query: 107 GGNPPAVPVVAQGSFSWTSPEGV-PISVNYVADENGYQPTGNAIPTSPPVPEQIARALAY 165
           GG+    P   Q SF++       P SVN V   +  +P  NA+     V    + AL +
Sbjct: 16  GGDLSHRPSNRQSSFTFHPAVNTKPKSVNLVTAVHAAEPARNAVSVKESVASSSSGALKW 75

Query: 166 IAKNIPLKK 174
             ++  LKK
Sbjct: 76  TPESWKLKK 84


>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
           extensin family protein similar to extensin-like protein
           [Lycopersicon esculentum] gi|5917664|gb|AAD55979;
           contains leucine-rich repeats, Pfam:PF00560; contains
           proline rich extensin domains, INTERPRO:IPR002965
          Length = 839

 Score = 27.1 bits (57), Expect = 7.0
 Identities = 14/46 (30%), Positives = 20/46 (43%)

Query: 111 PAVPVVAQGSFSWTSPEGVPISVNYVADENGYQPTGNAIPTSPPVP 156
           P+ P+      +  SP G P S + V       P+  + PTSP  P
Sbjct: 430 PSPPITVPSPPTTPSPGGSPPSPSIVPSPPSTTPSPGSPPTSPTTP 475


>At5g06340.1 68418.m00710 diadenosine 5',5'''-P1,P4-tetraphosphate
          hydrolase, putative similar to diadenosine
          5',5'''-P1,P4-tetraphosphate hydrolase from [Lupinus
          angustifolius] GI:1888557, [Hordeum vulgare subsp.
          vulgare] GI:2564253; contains Pfam profile PF00293:
          NUDIX domain
          Length = 227

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 1/40 (2%)

Query: 29 PYRYSTYRPFVSVTTPTPFLPIPISSVAPVRVVPKVSEGY 68
          P ++S  + F SV++P P + + ++  +P R V     GY
Sbjct: 24 PVKFSCLKQF-SVSSPKPLVVLSVALSSPARTVESPPVGY 62


>At3g53080.1 68416.m05850 galactose-binding lectin family protein
           contains Pfam domain PF02140: Galactose binding lectin
           domain
          Length = 155

 Score = 26.6 bits (56), Expect = 9.2
 Identities = 17/65 (26%), Positives = 27/65 (41%)

Query: 67  GYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSP 126
           GY    + F +  NP G+  +F   N G  A  + V +N  G      +V    F  +  
Sbjct: 81  GYVITKINFADYGNPTGTCGHFRRGNCGARATMRIVKKNCLGKEKCHLLVTDEMFGPSKC 140

Query: 127 EGVPI 131
           +G P+
Sbjct: 141 KGAPM 145


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.313    0.134    0.404 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,538,471
Number of Sequences: 28952
Number of extensions: 216585
Number of successful extensions: 434
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 407
Number of HSP's gapped (non-prelim): 33
length of query: 174
length of database: 12,070,560
effective HSP length: 76
effective length of query: 98
effective length of database: 9,870,208
effective search space: 967280384
effective search space used: 967280384
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 56 (26.6 bits)

- SilkBase 1999-2023 -