Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000312-TA|BGIBMGA000312-PA|IPR002483|Splicing factor PWI
(869 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 140 5e-33
At1g60200.1 68414.m06781 splicing factor PWI domain-containing p... 40 0.007
At3g60110.1 68416.m06712 DNA-binding bromodomain-containing prot... 35 0.27
At4g26630.1 68417.m03837 expressed protein 33 0.62
At5g49400.1 68418.m06113 zinc knuckle (CCHC-type) family protein... 33 0.82
At2g16860.1 68415.m01939 GCIP-interacting family protein similar... 32 1.4
At5g51680.1 68418.m06407 hydroxyproline-rich glycoprotein family... 32 1.9
At3g27250.1 68416.m03406 expressed protein 31 2.5
At5g39660.2 68418.m04803 Dof-type zinc finger domain-containing ... 31 3.3
At5g39660.1 68418.m04802 Dof-type zinc finger domain-containing ... 31 3.3
At4g39870.1 68417.m05649 expressed protein hypothetical protein,... 31 3.3
At3g28770.1 68416.m03591 expressed protein 31 3.3
At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical... 31 3.3
At2g45460.1 68415.m05654 forkhead-associated domain-containing p... 31 3.3
At1g76780.1 68414.m08935 expressed protein ; expression supporte... 31 3.3
At4g00710.1 68417.m00097 protein kinase family protein low simil... 31 4.4
At2g17580.1 68415.m02034 polynucleotide adenylyltransferase fami... 30 5.8
At1g56100.1 68414.m06442 pectinesterase inhibitor domain-contain... 30 5.8
At1g35820.1 68414.m04451 hypothetical protein 30 5.8
At4g37820.1 68417.m05351 expressed protein Kaposi's sarcoma-asso... 30 7.6
At1g75150.1 68414.m08729 expressed protein ; expression supporte... 30 7.6
At1g74325.1 68414.m08607 expressed protein ; expression supporte... 30 7.6
At1g43720.1 68414.m05023 hypothetical protein 30 7.6
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 140 bits (338), Expect = 5e-33
Identities = 68/141 (48%), Positives = 94/141 (66%), Gaps = 1/141 (0%)
Query: 4 YTGTSTEQDTRFSDKEKKLMKQMKFGDCLTQQVDMSKVKLDVLKPWITQKITEILNMEDD 63
+ GTS EQDTRFS+K+ KLMK KF L VD++KVK+DV+KPWI ++TE+L +ED+
Sbjct: 6 FRGTSAEQDTRFSNKQAKLMKSQKFAPELENLVDITKVKMDVMKPWIATRVTELLGIEDE 65
Query: 64 VVIEYVTNQLEEKFPCPKKMQINLTGFLNGKNARLFMGELWELLLSAQASENGIPESFTQ 123
V+I ++ L+ K K++QI LTGF+ KN FM ELW LLLSAQ + +G+P+ F
Sbjct: 66 VLINFIYGLLDGKVVNGKEIQITLTGFME-KNTGKFMKELWTLLLSAQNNPSGVPQQFLD 124
Query: 124 QKKEEIKKRMEEQQNKDKDKE 144
+ E KK+ EE K +E
Sbjct: 125 ARAAETKKKQEEANEIMKKRE 145
>At1g60200.1 68414.m06781 splicing factor PWI domain-containing
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF01480: PWI domain, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 899
Score = 39.9 bits (89), Expect = 0.007
Identities = 17/74 (22%), Positives = 44/74 (59%), Gaps = 3/74 (4%)
Query: 46 LKPWITQKITEILNMEDDVVIEYVTNQLEEKFPCPKKMQINLTGFLNGKNARLFMGELWE 105
++PWI++KI E L E+ +++++ + ++ + +++ L L+ + A +F+ ++W
Sbjct: 823 MRPWISKKIMEFLGEEEATLVDFIVSNTQQHVQASQMLEL-LQSILD-EEAEMFVLKMWR 880
Query: 106 LLL-SAQASENGIP 118
+L+ + E G+P
Sbjct: 881 MLIFEIKRVEAGVP 894
>At3g60110.1 68416.m06712 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 438
Score = 34.7 bits (76), Expect = 0.27
Identities = 19/74 (25%), Positives = 39/74 (52%), Gaps = 2/74 (2%)
Query: 465 DGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNED 524
D KT+S DD+EKE+ N ++ ++ + FL+R + + +K E + K Q ++
Sbjct: 298 DTKTESSDDDDDEKEE--NSKTEKKTVADKKKSVADFLKRIKKNSPQKGKETTSKNQKKN 355
Query: 525 EIKTKESKRAKRKA 538
+ K+ ++K+
Sbjct: 356 DGNVKKENDHQKKS 369
>At4g26630.1 68417.m03837 expressed protein
Length = 763
Score = 33.5 bits (73), Expect = 0.62
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Query: 476 EEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNEDEIKTKESKRAK 535
+E + + N+ + K ++T + E+D++K DE G E D K ESK AK
Sbjct: 204 DENKQVENVEGKEKEDKEENKTKEVEAAKAEVDESKVEDEKEGSEDENDNEKV-ESKDAK 262
>At5g49400.1 68418.m06113 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 275
Score = 33.1 bits (72), Expect = 0.82
Identities = 15/77 (19%), Positives = 38/77 (49%)
Query: 464 NDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNE 523
+ G++ S+++ + ED + SK + RR+ ++ S+ S + +
Sbjct: 197 SSGESSSEYSSSSDSEDERRRRRKAKKSKKKQKQRKERRRRYSSSSSESSESESASDSDS 256
Query: 524 DEIKTKESKRAKRKAHQ 540
DE +++ K++KR +++
Sbjct: 257 DEDRSRRKKKSKRHSNK 273
>At2g16860.1 68415.m01939 GCIP-interacting family protein similar to
GCIP-interacting protein mp29 (GI:27372623) [Mus
musculus]; similar to GCIP-interacting protein P29
(GI:11967379) [Homo sapiens]
Length = 298
Score = 32.3 bits (70), Expect = 1.4
Identities = 20/84 (23%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
Query: 455 VEKPPPEPFNDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSD 514
V + EP +D + + D +++EE+E+ P + + ++ R F R ++++ +KS+
Sbjct: 46 VHEQTREPLDDKRIEEDSSEEEEEEEDNQEPQV-DVTQLTGRQKKLFELRLKMNEARKSN 104
Query: 515 EVS-GKEQNEDEIKTKESKRAKRK 537
+ G E+ + E T+ +K+K
Sbjct: 105 QTDVGSEKKKMEAPTETKGISKQK 128
>At5g51680.1 68418.m06407 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965; similar to hydroxyproline-rich
Glycoprotein (HRGP) [Zea mays] gi|4007865|emb|CAA10387
Length = 343
Score = 31.9 bits (69), Expect = 1.9
Identities = 13/38 (34%), Positives = 22/38 (57%)
Query: 458 PPPEPFNDGKTQSDHTDDEEKEDIVNIPNLREYSKSLS 495
PPP + D +T SD DD + ++P+L ++S+S
Sbjct: 143 PPPPMYGDVETSSDIFDDASSDSFSSVPSLLATNRSVS 180
>At3g27250.1 68416.m03406 expressed protein
Length = 282
Score = 31.5 bits (68), Expect = 2.5
Identities = 22/58 (37%), Positives = 34/58 (58%), Gaps = 4/58 (6%)
Query: 466 GKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNE 523
GK++SD +DD E+ D+V + L E S +S SP L + +D + KS + G+E E
Sbjct: 76 GKSRSDLSDDREQTDVVTLQLLPEKS-DISGEYSP-LDQDSLDPSVKS--IIGEETQE 129
>At5g39660.2 68418.m04803 Dof-type zinc finger domain-containing
protein similar to H-protein promoter binding factor-2a
GI:3386546 from [Arabidopsis thaliana]
Length = 457
Score = 31.1 bits (67), Expect = 3.3
Identities = 20/66 (30%), Positives = 30/66 (45%)
Query: 465 DGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNED 524
DG +S+ EEK+ +LR S ++ T S + E K K++E SG
Sbjct: 68 DGGGESETDKKEEKDSECQEESLRNESNDVTTTTSGITEKTETTKAAKTNEESGGTACSQ 127
Query: 525 EIKTKE 530
E K K+
Sbjct: 128 EGKLKK 133
>At5g39660.1 68418.m04802 Dof-type zinc finger domain-containing
protein similar to H-protein promoter binding factor-2a
GI:3386546 from [Arabidopsis thaliana]
Length = 457
Score = 31.1 bits (67), Expect = 3.3
Identities = 20/66 (30%), Positives = 30/66 (45%)
Query: 465 DGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNED 524
DG +S+ EEK+ +LR S ++ T S + E K K++E SG
Sbjct: 68 DGGGESETDKKEEKDSECQEESLRNESNDVTTTTSGITEKTETTKAAKTNEESGGTACSQ 127
Query: 525 EIKTKE 530
E K K+
Sbjct: 128 EGKLKK 133
>At4g39870.1 68417.m05649 expressed protein hypothetical protein,
Schizosaccharomyces cerevisae, Z99168
Length = 394
Score = 31.1 bits (67), Expect = 3.3
Identities = 21/78 (26%), Positives = 37/78 (47%), Gaps = 7/78 (8%)
Query: 458 PPPEPFNDGKT---QSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSD 514
PPP P + ++ Q + T E+ +D+V+ P+ +S L L DK K D
Sbjct: 36 PPPLPDEEDESKRNQLESTTAEQPKDLVDEPDTSSFSAFL----GSLLSSDPKDKRKDQD 91
Query: 515 EVSGKEQNEDEIKTKESK 532
+++ EDE + E++
Sbjct: 92 PEDEEDEEEDEEEDSEAE 109
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 31.1 bits (67), Expect = 3.3
Identities = 17/70 (24%), Positives = 30/70 (42%)
Query: 467 KTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNEDEI 526
K +S H +KE E S +++E+DK +K +++ E E+
Sbjct: 1138 KKKSQHVKLVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKEM 1197
Query: 527 KTKESKRAKR 536
K E K+ K+
Sbjct: 1198 KESEEKKLKK 1207
>At3g20770.1 68416.m02627 ethylene-insensitive 3 (EIN3) identical to
ethylene-insensitive3 GI:2224933 from [Arabidopsis
thaliana]
Length = 628
Score = 31.1 bits (67), Expect = 3.3
Identities = 20/78 (25%), Positives = 36/78 (46%), Gaps = 8/78 (10%)
Query: 458 PPPEPFNDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVS 517
P P+ D + D+TDDE D E + + R R E DK K+ + +
Sbjct: 27 PVPQAEPDSIVEDDYTDDEIDVD--------ELERRMWRDKMRLKRLKEQDKGKEGVDAA 78
Query: 518 GKEQNEDEIKTKESKRAK 535
+ Q++++ + K+ RA+
Sbjct: 79 KQRQSQEQARRKKMSRAQ 96
>At2g45460.1 68415.m05654 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 915
Score = 31.1 bits (67), Expect = 3.3
Identities = 26/77 (33%), Positives = 37/77 (48%), Gaps = 3/77 (3%)
Query: 69 VTNQLEEKFPCPKKMQIN-LTGFLNGKNARLFMGELWELLLSAQASENGIPESFTQQKKE 127
V N+++E K MQ+ L L RL E LL AQ SE + + Q+K E
Sbjct: 412 VINKMKESEK-EKSMQVETLMSKLEDTRQRLVCSENRNRLLEAQVSEEQLAFADAQKKLE 470
Query: 128 EIKKRMEEQQNKDKDKE 144
E+ +++ Q KD D E
Sbjct: 471 ELDLQVKRLQ-KDLDSE 486
>At1g76780.1 68414.m08935 expressed protein ; expression supported by
MPSS
Length = 1871
Score = 31.1 bits (67), Expect = 3.3
Identities = 15/78 (19%), Positives = 39/78 (50%), Gaps = 2/78 (2%)
Query: 456 EKPPPEPFNDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDE 515
E P + G+ +DH ++E+KE+++ L S + + + + + K+S
Sbjct: 1058 ETYPKDKHTGGEDHNDHKEEEQKENVIAKAELNTEEDSFKKVEE--IEKQDHGELKRSMV 1115
Query: 516 VSGKEQNEDEIKTKESKR 533
+ +++ E++ KT+ ++
Sbjct: 1116 QAKRQETEEKDKTRAMEK 1133
>At4g00710.1 68417.m00097 protein kinase family protein low
similarity to protein kinase [Arabidopsis thaliana]
GI:2852449; contains Pfam profile: PF00069 Protein
kinase domain
Length = 489
Score = 30.7 bits (66), Expect = 4.4
Identities = 19/69 (27%), Positives = 36/69 (52%), Gaps = 4/69 (5%)
Query: 469 QSDHTDDEEKEDIVNIPNLREYS-KSLSRTPSPFLRRHEIDKN-KKSDEV--SGKEQNED 524
++ D+ E +I ++PN REY+ + L S F + + ++ +K+ V GK +N+
Sbjct: 22 EAPDVDNGESSEITDVPNFREYTLEQLKAATSGFAVEYIVSEHGEKAPNVVYKGKLENQK 81
Query: 525 EIKTKESKR 533
+I K R
Sbjct: 82 KIAVKRFTR 90
>At2g17580.1 68415.m02034 polynucleotide adenylyltransferase family
protein similar to SP|P13685 Poly(A) polymerase (EC
2.7.7.19) {Escherichia coli O157:H7}; contains Pfam
profile PF01743: polyA polymerase family protein
Length = 757
Score = 30.3 bits (65), Expect = 5.8
Identities = 17/81 (20%), Positives = 36/81 (44%), Gaps = 1/81 (1%)
Query: 456 EKPPPEPFNDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDE 515
E P EP ++ +DD+E +++P ++ + T +P + K K+
Sbjct: 631 EAPVSEPPKQKTSKRSRSDDQEAVGSLSVPAKIQHQSNKHDTNAPICELPK-QKTSKNHS 689
Query: 516 VSGKEQNEDEIKTKESKRAKR 536
++ +++ KE K AK+
Sbjct: 690 KESRKVKHNDLPVKEIKEAKQ 710
>At1g56100.1 68414.m06442 pectinesterase inhibitor domain-containing
protein contains TIGRFAM TIGR01614: pectinesterase
inhibitor domain; contains weak hit to Pfam PF04043:
Plant invertase/pectin methylesterase inhibitor
Length = 232
Score = 30.3 bits (65), Expect = 5.8
Identities = 19/60 (31%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Query: 473 TDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDE-VSGKEQNEDEIKTKES 531
TDD EKE I+++ N S+++ L+ + D+ KS++ KEQ + K ES
Sbjct: 128 TDDAEKEGIIDVVNAT--SEAIENETEVDLKEKDGDEEAKSEKPKKKKEQRKSRFKKMES 185
>At1g35820.1 68414.m04451 hypothetical protein
Length = 287
Score = 30.3 bits (65), Expect = 5.8
Identities = 15/74 (20%), Positives = 40/74 (54%)
Query: 464 NDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNE 523
+D ++QS H+ + +E +++ + E + S + S + + E+ + ++ +EV ++
Sbjct: 72 HDVQSQSQHSARQRREQLIHEHGVDEEGEDYSDSDSGNMPQTEVPETQEEEEVYRVTIDD 131
Query: 524 DEIKTKESKRAKRK 537
DEI + R +++
Sbjct: 132 DEIFQNSAARRQQR 145
>At4g37820.1 68417.m05351 expressed protein Kaposi's
sarcoma-associated herpes-like virus ORF73gene, Kaposi's
sarcoma-associated herpesvirus, U52064
Length = 532
Score = 29.9 bits (64), Expect = 7.6
Identities = 21/84 (25%), Positives = 41/84 (48%), Gaps = 3/84 (3%)
Query: 457 KPPPEPFNDGKTQSDHTDDEEKEDIVNIPNLREYS-KSLSRTPSPFLRRHEIDKN--KKS 513
K E + G+ + + + EKED + +E ++ + S +EI + K+
Sbjct: 337 KKKEESSSQGEGKEEEPEKREKEDSSSQEESKEEEPENKEKEASSSQEENEIKETEIKEK 396
Query: 514 DEVSGKEQNEDEIKTKESKRAKRK 537
+E S +E NE++ K+S ++RK
Sbjct: 397 EESSSQEGNENKETEKKSSESQRK 420
>At1g75150.1 68414.m08729 expressed protein ; expression supported
by MPSS
Length = 753
Score = 29.9 bits (64), Expect = 7.6
Identities = 20/71 (28%), Positives = 37/71 (52%), Gaps = 6/71 (8%)
Query: 467 KTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNEDEI 526
K+ S+ +D E +++I + E ++S P HE D +E+ K ++E+EI
Sbjct: 108 KSGSEISDSETRDEI----RVSETTESEKEDPDFETTVHEFDS--PMEELGEKGEDEEEI 161
Query: 527 KTKESKRAKRK 537
+ E+K A +K
Sbjct: 162 RVPETKEAGKK 172
>At1g74325.1 68414.m08607 expressed protein ; expression supported
by MPSS
Length = 179
Score = 29.9 bits (64), Expect = 7.6
Identities = 23/67 (34%), Positives = 32/67 (47%), Gaps = 4/67 (5%)
Query: 462 PFNDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQ 521
P K+ S EK+D+ +R +++SLSR P L H N KSD S +
Sbjct: 57 PLQVSKSNSFAWAKREKDDVC----VRVHNRSLSRGYIPSLSGHSPAFNGKSDVESKINK 112
Query: 522 NEDEIKT 528
+E E KT
Sbjct: 113 DEKEDKT 119
>At1g43720.1 68414.m05023 hypothetical protein
Length = 314
Score = 29.9 bits (64), Expect = 7.6
Identities = 15/73 (20%), Positives = 37/73 (50%)
Query: 464 NDGKTQSDHTDDEEKEDIVNIPNLREYSKSLSRTPSPFLRRHEIDKNKKSDEVSGKEQNE 523
+D ++QS H+ + +E +++ + E + S + S + E+ + ++ +EV ++
Sbjct: 8 HDVQSQSQHSARQRREQLIHEHGVDEEGEDYSDSDSGNMPEAEVPETQEEEEVYRVTIDD 67
Query: 524 DEIKTKESKRAKR 536
DE + A+R
Sbjct: 68 DESTARRGSSAQR 80
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.305 0.124 0.335
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,270,363
Number of Sequences: 28952
Number of extensions: 439296
Number of successful extensions: 1588
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1547
Number of HSP's gapped (non-prelim): 54
length of query: 869
length of database: 12,070,560
effective HSP length: 87
effective length of query: 782
effective length of database: 9,551,736
effective search space: 7469457552
effective search space used: 7469457552
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 43 (21.9 bits)
S2: 64 (29.9 bits)
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