BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000303-TA|BGIBMGA000303-PA|IPR005225|Small GTP-binding protein domain, IPR013753|Ras, IPR003577|Ras small GTPase, Ras type, IPR003579|Ras small GTPase, Rab type, IPR003578|Ras small GTPase, Rho type, IPR001806|Ras GTPase (209 letters) Database: mosquito 2123 sequences; 516,269 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value EF127647-1|ABL74413.1| 213|Anopheles gambiae Rab5 protein. 90 4e-20 AJ438610-3|CAD27475.1| 190|Anopheles gambiae putative RHO small... 72 1e-14 DQ182016-1|ABA56308.1| 353|Anopheles gambiae G(alpha)i protein. 25 1.7 AY724802-1|AAW50311.1| 134|Anopheles gambiae G protein alpha su... 25 1.7 AY724801-1|AAW50310.1| 134|Anopheles gambiae G protein alpha su... 25 1.7 AF457552-1|AAL68782.1| 311|Anopheles gambiae D7 protein long fo... 25 2.2 AB090818-2|BAC57912.1| 988|Anopheles gambiae reverse transcript... 23 5.1 AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein p... 23 8.9 >EF127647-1|ABL74413.1| 213|Anopheles gambiae Rab5 protein. Length = 213 Score = 90.2 bits (214), Expect = 4e-20 Identities = 61/174 (35%), Positives = 87/174 (50%), Gaps = 6/174 (3%) Query: 8 YRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTY-RQVISSNKTICTLQITDTTGSH 66 +++V+ G VGKSSLVLRFVKG F E TI + Q + + T +I DT G Sbjct: 25 FKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTLCIDDTTVKFEIWDTAGQE 84 Query: 67 QFPAMQRLSISKGHAFILVYSVSSRQSLEELKPIWQTIKEIKGVELPNIPVMLAGNKCDE 126 ++ ++ + A I+VY + + S K W +KE++ PNI + LAGNK D Sbjct: 85 RYHSLAPMYYRGAQAAIVVYDIQNSDSFARAK-TW--VKELQRQASPNIVIALAGNKADL 141 Query: 127 SPEIREVSASEGQAQAQAWGVSFMETSAKTNHNVTQLFQELL-NMEKNRNVSLQ 179 + R V E + A + FMETSAKT NV +F + + KN Q Sbjct: 142 A-NSRVVDYEEAKQYADDNRLLFMETSAKTAVNVNDIFLAIAKKLPKNEGAGPQ 194 >AJ438610-3|CAD27475.1| 190|Anopheles gambiae putative RHO small GTPase protein. Length = 190 Score = 71.7 bits (168), Expect = 1e-14 Identities = 53/172 (30%), Positives = 81/172 (47%), Gaps = 15/172 (8%) Query: 9 RVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSNKTICTLQITDTTGSHQF 68 + VV G G VGK+ +++ + +F Y+PT D Y + + +L + DT G + Sbjct: 8 KCVVVGDGTVGKTCMLISYTTDSFPGEYVPTSFDNYSAPMVVDGVQVSLGLWDTAGQEDY 67 Query: 69 PAMQRLSISKGHAFILVYSVSSRQSLEELKPIWQTIKEIKGVELPNIPVMLAGNKCD--E 126 ++ LS + F++ YSV+S S E + W EIK P+ P++L G K D E Sbjct: 68 DRLRPLSYPQTDVFLICYSVASPSSFENVTSKW--YPEIKH-HCPDAPIILVGTKIDLRE 124 Query: 127 SPEIREVSASEG-------QAQAQA---WGVSFMETSAKTNHNVTQLFQELL 168 E + A +G Q Q A V +ME SA T + Q+F E L Sbjct: 125 DRETISLLADQGLSALKREQGQKLANKIRAVKYMECSALTQRGLKQVFDEAL 176 >DQ182016-1|ABA56308.1| 353|Anopheles gambiae G(alpha)i protein. Length = 353 Score = 25.0 bits (52), Expect = 1.7 Identities = 14/47 (29%), Positives = 27/47 (57%), Gaps = 1/47 (2%) Query: 5 SNDYRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSN 51 +++ ++++ GAG GKS++V + +K Y + YR V+ SN Sbjct: 30 ASEVKLLLLGAGESGKSTIV-KQMKIIHETGYSQEECEQYRPVVYSN 75 >AY724802-1|AAW50311.1| 134|Anopheles gambiae G protein alpha subunit AgOn protein. Length = 134 Score = 25.0 bits (52), Expect = 1.7 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Query: 3 EQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSN 51 + + D ++++ GAG GKS++V + +K + YR V+ SN Sbjct: 16 QAAKDIKLLLLGAGESGKSTIV-KQMKIIHESGFTSEDFKQYRPVVYSN 63 >AY724801-1|AAW50310.1| 134|Anopheles gambiae G protein alpha subunit AgOa protein. Length = 134 Score = 25.0 bits (52), Expect = 1.7 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Query: 3 EQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSN 51 + + D ++++ GAG GKS++V + +K + YR V+ SN Sbjct: 16 QAAKDIKLLLLGAGESGKSTIV-KQMKIIHESGFTSEDFKQYRPVVYSN 63 >AF457552-1|AAL68782.1| 311|Anopheles gambiae D7 protein long form protein. Length = 311 Score = 24.6 bits (51), Expect = 2.2 Identities = 15/46 (32%), Positives = 20/46 (43%) Query: 122 NKCDESPEIREVSASEGQAQAQAWGVSFMETSAKTNHNVTQLFQEL 167 NK D PE+R V AS A + + +S K + F EL Sbjct: 237 NKSDLEPEVRSVLASCTGTHAYDYYSCLLNSSVKEDFRNAFYFHEL 282 >AB090818-2|BAC57912.1| 988|Anopheles gambiae reverse transcriptase protein. Length = 988 Score = 23.4 bits (48), Expect = 5.1 Identities = 15/43 (34%), Positives = 20/43 (46%) Query: 88 VSSRQSLEELKPIWQTIKEIKGVELPNIPVMLAGNKCDESPEI 130 VS + EELK I +++ K NIP M A PE+ Sbjct: 409 VSEEELAEELKEIARSLNPKKAPGDDNIPNMAAAAAILAFPEV 451 >AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein protein. Length = 3325 Score = 22.6 bits (46), Expect = 8.9 Identities = 7/31 (22%), Positives = 15/31 (48%) Query: 97 LKPIWQTIKEIKGVELPNIPVMLAGNKCDES 127 L +W + + G+ ++ +L +CD S Sbjct: 1988 LSVLWMVVHSVHGIMFKDLKQILRKEQCDAS 2018 Database: mosquito Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 516,269 Number of sequences in database: 2123 Lambda K H 0.314 0.128 0.358 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 177,958 Number of Sequences: 2123 Number of extensions: 5793 Number of successful extensions: 20 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 9 Number of HSP's gapped (non-prelim): 8 length of query: 209 length of database: 516,269 effective HSP length: 61 effective length of query: 148 effective length of database: 386,766 effective search space: 57241368 effective search space used: 57241368 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits) S2: 46 (22.6 bits)
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