BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000303-TA|BGIBMGA000303-PA|IPR005225|Small GTP-binding
protein domain, IPR013753|Ras, IPR003577|Ras small GTPase, Ras type,
IPR003579|Ras small GTPase, Rab type, IPR003578|Ras small GTPase, Rho
type, IPR001806|Ras GTPase
(209 letters)
Database: mosquito
2123 sequences; 516,269 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
EF127647-1|ABL74413.1| 213|Anopheles gambiae Rab5 protein. 90 4e-20
AJ438610-3|CAD27475.1| 190|Anopheles gambiae putative RHO small... 72 1e-14
DQ182016-1|ABA56308.1| 353|Anopheles gambiae G(alpha)i protein. 25 1.7
AY724802-1|AAW50311.1| 134|Anopheles gambiae G protein alpha su... 25 1.7
AY724801-1|AAW50310.1| 134|Anopheles gambiae G protein alpha su... 25 1.7
AF457552-1|AAL68782.1| 311|Anopheles gambiae D7 protein long fo... 25 2.2
AB090818-2|BAC57912.1| 988|Anopheles gambiae reverse transcript... 23 5.1
AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein p... 23 8.9
>EF127647-1|ABL74413.1| 213|Anopheles gambiae Rab5 protein.
Length = 213
Score = 90.2 bits (214), Expect = 4e-20
Identities = 61/174 (35%), Positives = 87/174 (50%), Gaps = 6/174 (3%)
Query: 8 YRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTY-RQVISSNKTICTLQITDTTGSH 66
+++V+ G VGKSSLVLRFVKG F E TI + Q + + T +I DT G
Sbjct: 25 FKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTLCIDDTTVKFEIWDTAGQE 84
Query: 67 QFPAMQRLSISKGHAFILVYSVSSRQSLEELKPIWQTIKEIKGVELPNIPVMLAGNKCDE 126
++ ++ + A I+VY + + S K W +KE++ PNI + LAGNK D
Sbjct: 85 RYHSLAPMYYRGAQAAIVVYDIQNSDSFARAK-TW--VKELQRQASPNIVIALAGNKADL 141
Query: 127 SPEIREVSASEGQAQAQAWGVSFMETSAKTNHNVTQLFQELL-NMEKNRNVSLQ 179
+ R V E + A + FMETSAKT NV +F + + KN Q
Sbjct: 142 A-NSRVVDYEEAKQYADDNRLLFMETSAKTAVNVNDIFLAIAKKLPKNEGAGPQ 194
>AJ438610-3|CAD27475.1| 190|Anopheles gambiae putative RHO small
GTPase protein.
Length = 190
Score = 71.7 bits (168), Expect = 1e-14
Identities = 53/172 (30%), Positives = 81/172 (47%), Gaps = 15/172 (8%)
Query: 9 RVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSNKTICTLQITDTTGSHQF 68
+ VV G G VGK+ +++ + +F Y+PT D Y + + +L + DT G +
Sbjct: 8 KCVVVGDGTVGKTCMLISYTTDSFPGEYVPTSFDNYSAPMVVDGVQVSLGLWDTAGQEDY 67
Query: 69 PAMQRLSISKGHAFILVYSVSSRQSLEELKPIWQTIKEIKGVELPNIPVMLAGNKCD--E 126
++ LS + F++ YSV+S S E + W EIK P+ P++L G K D E
Sbjct: 68 DRLRPLSYPQTDVFLICYSVASPSSFENVTSKW--YPEIKH-HCPDAPIILVGTKIDLRE 124
Query: 127 SPEIREVSASEG-------QAQAQA---WGVSFMETSAKTNHNVTQLFQELL 168
E + A +G Q Q A V +ME SA T + Q+F E L
Sbjct: 125 DRETISLLADQGLSALKREQGQKLANKIRAVKYMECSALTQRGLKQVFDEAL 176
>DQ182016-1|ABA56308.1| 353|Anopheles gambiae G(alpha)i protein.
Length = 353
Score = 25.0 bits (52), Expect = 1.7
Identities = 14/47 (29%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Query: 5 SNDYRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSN 51
+++ ++++ GAG GKS++V + +K Y + YR V+ SN
Sbjct: 30 ASEVKLLLLGAGESGKSTIV-KQMKIIHETGYSQEECEQYRPVVYSN 75
>AY724802-1|AAW50311.1| 134|Anopheles gambiae G protein alpha
subunit AgOn protein.
Length = 134
Score = 25.0 bits (52), Expect = 1.7
Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Query: 3 EQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSN 51
+ + D ++++ GAG GKS++V + +K + YR V+ SN
Sbjct: 16 QAAKDIKLLLLGAGESGKSTIV-KQMKIIHESGFTSEDFKQYRPVVYSN 63
>AY724801-1|AAW50310.1| 134|Anopheles gambiae G protein alpha
subunit AgOa protein.
Length = 134
Score = 25.0 bits (52), Expect = 1.7
Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Query: 3 EQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRESYIPTIEDTYRQVISSN 51
+ + D ++++ GAG GKS++V + +K + YR V+ SN
Sbjct: 16 QAAKDIKLLLLGAGESGKSTIV-KQMKIIHESGFTSEDFKQYRPVVYSN 63
>AF457552-1|AAL68782.1| 311|Anopheles gambiae D7 protein long form
protein.
Length = 311
Score = 24.6 bits (51), Expect = 2.2
Identities = 15/46 (32%), Positives = 20/46 (43%)
Query: 122 NKCDESPEIREVSASEGQAQAQAWGVSFMETSAKTNHNVTQLFQEL 167
NK D PE+R V AS A + + +S K + F EL
Sbjct: 237 NKSDLEPEVRSVLASCTGTHAYDYYSCLLNSSVKEDFRNAFYFHEL 282
>AB090818-2|BAC57912.1| 988|Anopheles gambiae reverse transcriptase
protein.
Length = 988
Score = 23.4 bits (48), Expect = 5.1
Identities = 15/43 (34%), Positives = 20/43 (46%)
Query: 88 VSSRQSLEELKPIWQTIKEIKGVELPNIPVMLAGNKCDESPEI 130
VS + EELK I +++ K NIP M A PE+
Sbjct: 409 VSEEELAEELKEIARSLNPKKAPGDDNIPNMAAAAAILAFPEV 451
>AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein
protein.
Length = 3325
Score = 22.6 bits (46), Expect = 8.9
Identities = 7/31 (22%), Positives = 15/31 (48%)
Query: 97 LKPIWQTIKEIKGVELPNIPVMLAGNKCDES 127
L +W + + G+ ++ +L +CD S
Sbjct: 1988 LSVLWMVVHSVHGIMFKDLKQILRKEQCDAS 2018
Database: mosquito
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 516,269
Number of sequences in database: 2123
Lambda K H
0.314 0.128 0.358
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 177,958
Number of Sequences: 2123
Number of extensions: 5793
Number of successful extensions: 20
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 9
Number of HSP's gapped (non-prelim): 8
length of query: 209
length of database: 516,269
effective HSP length: 61
effective length of query: 148
effective length of database: 386,766
effective search space: 57241368
effective search space used: 57241368
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 46 (22.6 bits)
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