Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000300-TA|BGIBMGA000300-PA|IPR005835|Nucleotidyl
transferase
(451 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4D7.09 |tif223||translation initiation factor eIF2B|Schizosa... 120 6e-28
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 70 6e-13
SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc... 41 4e-04
SPBC13G1.02 |||mannose-1-phosphate guanyltransferase |Schizosacc... 32 0.19
SPBC16A3.06 |||tRNA specific adenosine deaminase |Schizosaccharo... 31 0.33
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 31 0.44
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 2.3
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 28 2.3
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 27 4.1
SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb... 27 4.1
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 27 4.1
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom... 27 5.4
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 27 7.1
SPAC10F6.07c |mug94||sequence orphan|Schizosaccharomyces pombe|c... 27 7.1
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 27 7.1
SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|... 27 7.1
SPBC21C3.02c |sds3||Sds3-like family protein|Schizosaccharomyces... 27 7.1
>SPAC4D7.09 |tif223||translation initiation factor
eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 468
Score = 120 bits (288), Expect = 6e-28
Identities = 109/417 (26%), Positives = 201/417 (48%), Gaps = 51/417 (12%)
Query: 5 LEFQVVVLAAGKGSRMPDVGG-SVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVLDED 63
+EFQ VV A S P G ++ K LLP+G P+L YPL LE GF +++ ++E
Sbjct: 37 IEFQAVVFAGFGNSLYPLTGSDALPKALLPIGNKPMLHYPLYWLEAAGFTSAILICMEEA 96
Query: 64 KSNI---LNALEKCPLKIKYELIVIPSEEDWGTANSLKHVSARINT----------DLLV 110
+++I L + + ++I E I ++ +A++L+ VS I + D +
Sbjct: 97 EAHINAWLRSGYEGHMRIHVEAPTI-LDDSKSSADALRAVSHLIKSQKMLTDTHQNDFVC 155
Query: 111 ISGDLITNINLNDVLNLHRKHDACVTTLFFNNGPEEWIELPGPKTKSKPDRDLVCIDKET 170
+S D I + L+ R + ++ P E ++K + L+ I+++T
Sbjct: 156 LSCDSIVGLPPIYGLDKFRLDNPSALAVY---SPVLKYEHITSQSKEIDAKQLIGIEEKT 212
Query: 171 ERLVFLASASDFEENVTIPRLLVKKYDALSIYSRLLDAHVYVMKHWILDYIVDSEKFTSI 230
RL++ S++D + T L+ K+ +++ + L DAH++V KHW++D I + E +SI
Sbjct: 213 SRLLYAKSSADVGSDFTFRMSLLWKHPRVTLNTNLSDAHIFVFKHWVIDLIREKESISSI 272
Query: 231 KGEVVPYIVKKQLTKPNNLVEKKGTSEKNAEINKGIFDYAIETGYERKIREISAYNDHKH 290
+G+++PY+VK Q +K T +N + R +S+ N+ +
Sbjct: 273 RGDLIPYLVKCQ-------YQKSFTVRENIQ------------------RFLSSPNNIDN 307
Query: 291 GNKGVYFNDTLRCYAHIPSKNTFAIRVNTLSSFYLSNNKILSKWQDLTGSSLFERFHPNS 350
+ G+ + ++ A I R N L + Y NK ++K LT +R +
Sbjct: 308 YDGGL-SSQEIKINALIAKDGIICSRANNLPN-YFELNKCIAK---LTPE---QRLVDVT 359
Query: 351 EVKTIQIDDNCTVGEKTIINEKTSVKNSFIGSNCNIENKVRLTNCILMNNVTIKESL 407
+ + +C V E T I + +++K S IG NC I V ++N ILM+N+ +++ +
Sbjct: 360 VSERALVGADCMVNEGTTIKDNSNIKKSIIGKNCVIGKGVVVSNSILMDNIVVEDGV 416
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 70.1 bits (164), Expect = 6e-13
Identities = 82/410 (20%), Positives = 171/410 (41%), Gaps = 42/410 (10%)
Query: 8 QVVVLAAGKGSRMPDVGGSVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVLDEDKSNI 67
Q +VL+ R + +CLLP+ P++ Y L G Q+V + I
Sbjct: 19 QAIVLSDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAGVQEVYVFCCAH-AGQI 77
Query: 68 LNALEKCPLKIK---YELIVIPSEEDWGTANSLKHVSAR--INTDLLVISGDLITNINLN 122
+EK + + + I S E ++L+ + ++ I +D +++SGD+++N+ LN
Sbjct: 78 REYIEKSKWNLPSSPFSVNTIVSRESLSVGDALRELDSKQLITSDFILVSGDVVSNVPLN 137
Query: 123 DVLNLHRKHDACVTTLFFNNGPEEWIELPGPKTKSKPDRDLVCIDKETERLVFLASASDF 182
+VL HRK E P +T+++ + + IDK+T + V +
Sbjct: 138 EVLKEHRKRREDDKNAIMTMVVRE--ASPFHRTRARTESSVFVIDKKTSQCVHYQANERG 195
Query: 183 EENVTIPRLLVKKYDALSIYSRLLDAHVYVMKHWILDYIVDSEKFTSIKGEVVPYIVKKQ 242
+ V++ + +++ L + + L+D + + + + ++ + I+ + V ++
Sbjct: 196 KHYVSMDPEIFNEHEELEVRNDLIDCQIDICSNDVPALFTENFDYQDIRKDFVYGVLTSD 255
Query: 243 LTKPNNLVEKKGTSEKNAEINKGIFDYAIETGYERKIREISAYNDHKHGNKGVYFNDTLR 302
L + K I + + Y ++R + Y+ +K V +
Sbjct: 256 L------------------LGKKIHCHVAKENYAARVRSLQTYDAI---SKDVL---SRW 291
Query: 303 CYAHIPSKN----TFAIRVNTL---SSFYLSNNKILSKWQDLTGSSLFERFHPNSEVKTI 355
Y +P N TF+ + + + L+ + I+ K + L G+ + + S V
Sbjct: 292 VYPFVPDSNLLNQTFSYQRHQIYKEEDVVLARSCII-KARTLIGA--YTKVGDASVVANT 348
Query: 356 QIDDNCTVGEKTIINEKTSVKNSFIGSNCNIENKVRLTNCILMNNVTIKE 405
I NCT+G I+ ++ IG NC I + + + NN +I++
Sbjct: 349 IIGRNCTIGSNCSIDSAFLWEDVVIGDNCRIGKAILANSVKIGNNCSIED 398
Score = 37.9 bits (84), Expect = 0.003
Identities = 29/128 (22%), Positives = 59/128 (46%), Gaps = 16/128 (12%)
Query: 294 GVYFNDTL--RCYAHIPSKNTFAIRVNTLSSFYLSNNKILSKW-------QDLTGSSLFE 344
GV +D L + + H+ +N +A RV +L ++ + +LS+W +L +
Sbjct: 250 GVLTSDLLGKKIHCHVAKEN-YAARVRSLQTYDAISKDVLSRWVYPFVPDSNLLNQTFSY 308
Query: 345 RFHPNSEVKTIQIDDNCTVGEKTIINEKTSV------KNSFIGSNCNIENKVRLTNCILM 398
+ H + + + + +C + +T+I T V N+ IG NC I + + + L
Sbjct: 309 QRHQIYKEEDVVLARSCIIKARTLIGAYTKVGDASVVANTIIGRNCTIGSNCSIDSAFLW 368
Query: 399 NNVTIKES 406
+V I ++
Sbjct: 369 EDVVIGDN 376
Score = 32.3 bits (70), Expect = 0.14
Identities = 20/57 (35%), Positives = 33/57 (57%), Gaps = 6/57 (10%)
Query: 355 IQIDDNCTVGEKTIINEKTSVKNSFIGSNCNIENKVRLTNCILMNNVTIKESLAQLT 411
+ I DNC +G+ + N SVK IG+NC+IE+ + +++ + TI E +LT
Sbjct: 371 VVIGDNCRIGKAILAN---SVK---IGNNCSIEDGAIVAAGVVIGDNTIIEKNKRLT 421
>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 363
Score = 40.7 bits (91), Expect = 4e-04
Identities = 28/129 (21%), Positives = 60/129 (46%), Gaps = 4/129 (3%)
Query: 8 QVVVLAAGKGSRMPDVGGSVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVLDEDKSNI 67
+ ++L G G+R+ + ++ K L+ G P++ + + L G D+++ V + +
Sbjct: 2 KALILVGGFGTRLRPLTLTLPKPLVEFGNKPMILHQVEALAAAGVTDIVLAV-NYRPEIM 60
Query: 68 LNALEKCPLKIKYELIVIPSEEDWGTANSL---KHVSARINTDLLVISGDLITNINLNDV 124
+ AL+K + + E GTA L + + A+ ++ V++ D+I D+
Sbjct: 61 VEALKKYEKEYNVNITFSVENEPLGTAGPLALARDILAKDHSPFFVLNSDVICEYPFADL 120
Query: 125 LNLHRKHDA 133
H+ H A
Sbjct: 121 AAFHKAHGA 129
>SPBC13G1.02 |||mannose-1-phosphate guanyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 414
Score = 31.9 bits (69), Expect = 0.19
Identities = 17/58 (29%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Query: 347 HPNSEV-KTIQIDDNCTVGEKTIINEKTSVKNSFIGSNCNIENKVRLTNCILMNNVTI 403
HPN+ V K +I N ++G + I + ++NS I +C I + + IL + I
Sbjct: 291 HPNAIVSKGAKIGPNVSIGARVRIEDGARIRNSIIQEDCEISANAVVLHSILSRHCKI 348
Score = 27.5 bits (58), Expect = 4.1
Identities = 12/42 (28%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Query: 367 TIINEKTSVKN-SFIGSNCNIENKVRLTNCILMNNVTIKESL 407
TI+ V+ + +G++C + ++VR+ NC+++ + IK L
Sbjct: 367 TIMRNSVKVQAITVMGADCIVHDEVRVQNCLVLPHKEIKVGL 408
>SPBC16A3.06 |||tRNA specific adenosine deaminase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 31.1 bits (67), Expect = 0.33
Identities = 24/92 (26%), Positives = 41/92 (44%), Gaps = 6/92 (6%)
Query: 142 NGPEEWIELPGPKTKSKPDRDLVCIDKETERLVFLASASDFEENVTIPRLLVKKYDALSI 201
NG EW L G + K + + +C+ T A E+ ++ + AL
Sbjct: 38 NGVREWTTLAGVVIQKKMENEFICVCLATGVKCTPAGIIKNEQLGSVLHDCHAEILALRC 97
Query: 202 YSRLLDAHVYVMKH-----WILDYIVDSEKFT 228
++RLL H ++K W+L+ + D+ KFT
Sbjct: 98 FNRLLLEHCILIKESKKDTWLLE-VADNGKFT 128
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 30.7 bits (66), Expect = 0.44
Identities = 33/149 (22%), Positives = 63/149 (42%), Gaps = 8/149 (5%)
Query: 87 SEEDWGTAN-SLKHVSARINTDLLVISGDLITNINLNDVLNLHRKHDAC-VTTLFFNNGP 144
S ++ T+N L H+ N ++IS ++ N++ L H A + NN
Sbjct: 27 SSQNSTTSNPELSHIEKESNASSIIISQQPLSPSNISSAAPLDETHIATKASASLRNNNV 86
Query: 145 EEWIELPGP-KTKSKPDRDLVCIDK---ETERLVFLA-SASDFEENVTIPRLLVKKYDAL 199
I P + S D D +D+ ++E + ++ S F E++ + R ++ L
Sbjct: 87 SPHIPSPSSFSSSSSSDLDKSMLDEKHPDSEDITAVSLSTPPFPESIDVARFSSEEKKIL 146
Query: 200 SIYSRLLDAHVYVMKHWILDYIVDSEKFT 228
S R LD + + WI ++ S ++
Sbjct: 147 SRIRRKLDLRI-ITCLWITYFLSRSVTYS 174
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 28.3 bits (60), Expect = 2.3
Identities = 19/72 (26%), Positives = 32/72 (44%), Gaps = 8/72 (11%)
Query: 330 ILSKWQDLTGSSLFERFHPNSEVKTIQIDDNCTVGEKTIINEKTSVKNSFIGSNCNIENK 389
+LSK+ T FE + + +Q D++ + K +N GSN + +
Sbjct: 704 VLSKYTQWTTQKFFEEANKQDDTSEVQNDNSSSWFSKA--------RNILSGSNEVLPFR 755
Query: 390 VRLTNCILMNNV 401
T CIL+NN+
Sbjct: 756 FSPTMCILLNNI 767
>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 674
Score = 28.3 bits (60), Expect = 2.3
Identities = 31/122 (25%), Positives = 54/122 (44%), Gaps = 5/122 (4%)
Query: 1 MHKILEFQVVVLAAGKGSRMPDVGGSVSKCLLPVGPYPVLWYPLNMLEKIGFQDVMIVVL 60
+ ++ F V AG +R V + L V P+ V+ L+M G + ++
Sbjct: 125 LKRLQRFLVSENEAGNINRQEAVS-MLPPLFLDVEPHHVI---LDMCAAPGSKTAQLIEA 180
Query: 61 DEDKSNILNALEKCPLKIKYELIVIPSEEDWGTANSLKHVSARINT-DLLVISGDLITNI 119
K+NI +A E +VI ++ D A L H R+N+ ++LV++ D T
Sbjct: 181 VYKKANIKDAAHDSKNLKSVEGLVIANDADPKRAQMLVHQINRLNSPNILVVNHDASTMP 240
Query: 120 NL 121
N+
Sbjct: 241 NI 242
>SPAPJ696.01c |vps17||retromer complex subunit
Vps17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 549
Score = 27.5 bits (58), Expect = 4.1
Identities = 34/149 (22%), Positives = 63/149 (42%), Gaps = 15/149 (10%)
Query: 252 KKGTSEKNAEINKGIFDYAIETGYE----------RKIREISAYNDHKHGNKGVYFNDTL 301
K+ + +A++ + + DY++E + + ++ IS + + V D+L
Sbjct: 271 KQSLALTHADLGQSLIDYSVEEQHNGLANALNRVGKMLQAISDVRIMQSSKQLVTLADSL 330
Query: 302 RCYAHIPSKNTFAIRVNTLSSFYLSNNKILSKWQDLTGSSLFERFHPNSEVKTIQIDDNC 361
CYA S N F ++ + L + I SK Q + S R + ++ + DD
Sbjct: 331 -CYA---SDNAFVVKEILSNRHILMRDLISSKNQTNSYLSAANRLQDSPKISKARTDDAL 386
Query: 362 TVGEKTIINEK-TSVKNSFIGSNCNIENK 389
E ++EK S K F+ N E+K
Sbjct: 387 QALEVARVHEKLLSDKVDFVTLNLVKESK 415
>SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 27.5 bits (58), Expect = 4.1
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
Query: 48 EKIGFQDVMIVVLD----EDKSNILNALEKCPLKIKYELIVIPSEED 90
EK+ + ++ VVL+ ED +N+ + + KC L I E + IP+ D
Sbjct: 335 EKVAYGTLVQVVLEDWPLEDFNNLASFMAKCHLPITLEELGIPNVTD 381
>SPCC777.14 |prp4||serine/threonine protein kinase
Prp4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 477
Score = 27.5 bits (58), Expect = 4.1
Identities = 17/44 (38%), Positives = 22/44 (50%)
Query: 158 KPDRDLVCIDKETERLVFLASASDFEENVTIPRLLVKKYDALSI 201
KPD LV + ++ L SASD EN P L+ + Y A I
Sbjct: 288 KPDNMLVNEKRNILKICDLGSASDASENEITPYLVSRFYRAPEI 331
>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 601
Score = 27.1 bits (57), Expect = 5.4
Identities = 14/43 (32%), Positives = 23/43 (53%)
Query: 156 KSKPDRDLVCIDKETERLVFLASASDFEENVTIPRLLVKKYDA 198
K K D+D ++ E + L + SD E V +P L + K+D+
Sbjct: 102 KLKLDKDSSSVNYENQISKPLITFSDIHETVNVPFLCLDKHDS 144
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 26.6 bits (56), Expect = 7.1
Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 1/52 (1%)
Query: 308 PSKNTFAIRVNTLSSFYLSNNKILSKWQDLTGSSLFERFHPNSEVKTIQIDD 359
PS+N + N L + +L +KIL + T L ER + I +DD
Sbjct: 101 PSQNAYEADKNALDAKFLEAHKILENLDEQT-RKLSERTEKQQDELDIALDD 151
>SPAC10F6.07c |mug94||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 188
Score = 26.6 bits (56), Expect = 7.1
Identities = 16/87 (18%), Positives = 38/87 (43%), Gaps = 4/87 (4%)
Query: 315 IRVNTLSSFYLSNNKILSKWQDLTGSSLFERFHPNSEVKTIQIDDNCTVGEKTIINEKTS 374
+ N L + + +KI+ +++ ++LFER +S K+ + ++N +
Sbjct: 41 VLANLLGGIFENEDKIIPNYKEAVIAALFERLLLSSNQKSTSKSNQMI----KLLNNSLA 96
Query: 375 VKNSFIGSNCNIENKVRLTNCILMNNV 401
N CN ++K+ + + N +
Sbjct: 97 HNNGLPSQRCNHDSKLVYMSLVQNNGL 123
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 26.6 bits (56), Expect = 7.1
Identities = 12/27 (44%), Positives = 14/27 (51%)
Query: 136 TTLFFNNGPEEWIELPGPKTKSKPDRD 162
T LF N P+EW L KS P +D
Sbjct: 187 TLLFSNEIPQEWCSLELSYYKSSPSKD 213
>SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 381
Score = 26.6 bits (56), Expect = 7.1
Identities = 13/58 (22%), Positives = 26/58 (44%)
Query: 330 ILSKWQDLTGSSLFERFHPNSEVKTIQIDDNCTVGEKTIINEKTSVKNSFIGSNCNIE 387
++S W DLT + + N + I + G+ + NE++ +S + N N +
Sbjct: 230 LISPWLDLTNAKKIGSYRANDGLDVICYETLNRFGKAYVNNEESLFTSSVVNINMNCD 287
>SPBC21C3.02c |sds3||Sds3-like family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 491
Score = 26.6 bits (56), Expect = 7.1
Identities = 27/135 (20%), Positives = 61/135 (45%), Gaps = 17/135 (12%)
Query: 250 VEKKGTSEKNAEINKGIFDYAIETGYERKIREISAYNDHKHGNKGVYFNDTLRCYAHIPS 309
V +K ++ ++I + A+ET E REI+ D + ++ + D + I
Sbjct: 256 VSRKWSTNSESKIKR----VALETSQEESDREIA---DRRSASEQAHEADDEKA---IKR 305
Query: 310 KNTFAIRVNTLSSFYLSNNKI-------LSKWQDLTGSSLFERFHPNSEVKTIQIDDNCT 362
K F +N + F N++ L++ +++ + ERF+ ++ T + DD
Sbjct: 306 KEAFDALLNIETEFTFLRNRLYGKKLLKLNEHEEMIQNETHERFNACIDLITERRDDRVR 365
Query: 363 VGEKTIINEKTSVKN 377
+ + ++ + ++KN
Sbjct: 366 LATENLMKQLGNIKN 380
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.135 0.393
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,206,092
Number of Sequences: 5004
Number of extensions: 99447
Number of successful extensions: 295
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 274
Number of HSP's gapped (non-prelim): 29
length of query: 451
length of database: 2,362,478
effective HSP length: 75
effective length of query: 376
effective length of database: 1,987,178
effective search space: 747178928
effective search space used: 747178928
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 55 (26.2 bits)
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