Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000299-TA|BGIBMGA000299-PA|undefined
(286 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g54670.1 68418.m06807 kinesin-like protein C (KATC) 33 0.29
At1g27750.1 68414.m03391 ubiquitin system component Cue domain-c... 33 0.29
At5g35790.1 68418.m04292 glucose-6-phosphate 1-dehydrogenase / G... 31 0.67
At3g04050.1 68416.m00427 pyruvate kinase, putative similar to py... 31 0.89
At2g06530.1 68415.m00724 SNF7 family protein contains Pfam domai... 31 0.89
At3g55810.1 68416.m06201 pyruvate kinase, putative similar to py... 31 1.2
At3g55650.1 68416.m06183 pyruvate kinase, putative simlar to pyr... 30 1.6
At1g68790.1 68414.m07863 expressed protein 29 2.7
At1g15910.1 68414.m01908 XH/XS domain-containing protein / XS zi... 29 2.7
At3g25960.1 68416.m03235 pyruvate kinase, putative similar to py... 29 3.6
At1g05260.1 68414.m00532 peroxidase 3 (PER3) (P3) / rare cold-in... 29 4.7
At5g43880.1 68418.m05366 expressed protein 28 6.3
At1g74290.1 68414.m08603 esterase/lipase/thioesterase family pro... 28 6.3
At1g09470.1 68414.m01059 expressed protein ; expression supporte... 28 6.3
At3g45850.1 68416.m04962 kinesin motor protein-related kinesin-r... 28 8.3
>At5g54670.1 68418.m06807 kinesin-like protein C (KATC)
Length = 754
Score = 32.7 bits (71), Expect = 0.29
Identities = 35/172 (20%), Positives = 66/172 (38%), Gaps = 6/172 (3%)
Query: 1 MTNYQEQLTQMQHDLESGLDNMAETLANAGGDTTAVTE----LNGHFSNLQFDLENMFLS 56
+ Y L L+ LD ET+ + TA+ E L G FS LQ L S
Sbjct: 201 LQEYNSSLQLYNSKLQGDLDEAHETIKRGEKERTAIIENIGNLKGQFSALQEQLAASKAS 260
Query: 57 QKELNDNQNKLSVDLEGLWQPIEVLRSTKADREEVADALRDKAGIAALNGLVSLQQFDAV 116
Q+++ + +L ++ L ++ ++ + DR V + ++ + +
Sbjct: 261 QEDIMKQKGELVNEIASLKVELQQVKDDR-DRHLVEVKTLQTEATKYNDFKDAITELETT 319
Query: 117 QGDFEKRILTAYDKFNNQEMIWQKAIDDLFRGLNEKADYVQ-VTSLRDEIQK 167
+I D+ N E Q + F +NE D Q + L+ +++
Sbjct: 320 CSSQSTQIRQLQDRLVNSERRLQVSDLSTFEKMNEYEDQKQSIIDLKSRVEE 371
>At1g27750.1 68414.m03391 ubiquitin system component Cue
domain-containing protein very low similarity to ASC-1
complex subunit P100 [Homo sapiens] GI:12061187; contains
Pfam profile PF02845: CUE domain
Length = 1973
Score = 32.7 bits (71), Expect = 0.29
Identities = 21/71 (29%), Positives = 37/71 (52%), Gaps = 4/71 (5%)
Query: 175 RINAMSEILGEPRAAAILRKIHRDSACL----SCATPAVMDLEEPGTIPALPALSSRSPG 230
R++ E + +P++A LR + L + ++ + + E+P T P+LPA S+ S
Sbjct: 1675 RLDTSLETMPQPKSAPTLRSKDKGKGKLIESDTSSSASAIYTEKPITRPSLPASSASSAT 1734
Query: 231 VGAESLNPKED 241
VG PK+D
Sbjct: 1735 VGRFVRKPKDD 1745
>At5g35790.1 68418.m04292 glucose-6-phosphate 1-dehydrogenase / G6PD
(APG1) identical to SP|Q43727 Glucose-6-phosphate
1-dehydrogenase, cytoplasmic isoform (EC 1.1.1.49)
(G6PD) {Arabidopsis thaliana}
Length = 576
Score = 31.5 bits (68), Expect = 0.67
Identities = 37/134 (27%), Positives = 59/134 (44%), Gaps = 8/134 (5%)
Query: 112 QFDAVQGD-FEKRILTAYDKFNNQEMIWQKAIDDLFRGLNEKADYVQVTSLRDEIQK-YL 169
QF V G+ ++K T D N+ +I + + ++ +N K + + R ++ Y
Sbjct: 434 QFRHVPGNLYKKSFATNLDNATNELVIRVQPDEGIYLRINNKVPGLGMRLDRSDLNLLYR 493
Query: 170 NMFDSRI-NAMSEILG---EPRAAAILRKIHRDSACLSCATPAVMDLEEPGTIPALPALS 225
+ + I +A +L E +R D+A TPA+ +LEE IP L
Sbjct: 494 SRYPREIPDAYERLLLDAIEGERRLFIRSDELDAAW-DLFTPALKELEEKKIIPELYPYG 552
Query: 226 SRSPGVGAESLNPK 239
SR P VGA L K
Sbjct: 553 SRGP-VGAHYLASK 565
>At3g04050.1 68416.m00427 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 510
Score = 31.1 bits (67), Expect = 0.89
Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 4/65 (6%)
Query: 46 LQFDLENMFLSQKELNDNQNKLSVDLEGLWQPIEVL----RSTKADREEVADALRDKAGI 101
++ +E MFL+QK + N L + Q +E + R T+A+ +VA+A+ D
Sbjct: 265 MEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKSPRPTRAEATDVANAVLDGTDC 324
Query: 102 AALNG 106
L+G
Sbjct: 325 VMLSG 329
>At2g06530.1 68415.m00724 SNF7 family protein contains Pfam domain,
PF03357: SNF7 family
Length = 225
Score = 31.1 bits (67), Expect = 0.89
Identities = 31/124 (25%), Positives = 55/124 (44%), Gaps = 10/124 (8%)
Query: 66 KLSVDLEGLWQPIEVLRSTKADREEVADALRDKAGIAALNGLVSLQQFDAVQGDFEKRIL 125
KL L+G+ I+ L+ST+A E + + + +N ++L + +FE++
Sbjct: 79 KLKSQLQGVSLRIQTLKSTQAMGEAMKGVTK---AMGQMNRQMNLPSLQKIMQEFERQ-- 133
Query: 126 TAYDKFNNQEMIWQKAIDDLFRGLNEKADYVQ-VTSLRDEIQKYLNMFDSRINAMSEILG 184
+K + AIDD G E+ + V+ + DEI +N +NA S +
Sbjct: 134 --NEKMEMVSEVMGDAIDDALEGDEEEEETEDLVSQVLDEIGIDIN--QELVNAPSGAVA 189
Query: 185 EPRA 188
P A
Sbjct: 190 VPAA 193
>At3g55810.1 68416.m06201 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 492
Score = 30.7 bits (66), Expect = 1.2
Identities = 25/95 (26%), Positives = 44/95 (46%), Gaps = 9/95 (9%)
Query: 16 ESGLDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFLSQKELNDNQNKLSVDLEGLW 75
+ G+ N + L N+ A +L ++ +E MFL+QK + N L +
Sbjct: 222 QEGVMNCEKILENSDAFMVARGDLG-----MEIQIEKMFLAQKTMIKMANALGKPVVTAT 276
Query: 76 QPIEVL----RSTKADREEVADALRDKAGIAALNG 106
Q +E + R T+A+ +VA+A+ D L+G
Sbjct: 277 QMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSG 311
>At3g55650.1 68416.m06183 pyruvate kinase, putative simlar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 510
Score = 30.3 bits (65), Expect = 1.6
Identities = 25/95 (26%), Positives = 44/95 (46%), Gaps = 9/95 (9%)
Query: 16 ESGLDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFLSQKELNDNQNKLSVDLEGLW 75
+ G+ N + L N+ A +L ++ +E MFL+QK + N L +
Sbjct: 240 QEGVMNCEKILENSDAFMVARGDLG-----MEIPIEKMFLAQKTMIKMANALGKPVVTAT 294
Query: 76 QPIEVL----RSTKADREEVADALRDKAGIAALNG 106
Q +E + R T+A+ +VA+A+ D L+G
Sbjct: 295 QMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSG 329
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 29.5 bits (63), Expect = 2.7
Identities = 32/141 (22%), Positives = 59/141 (41%), Gaps = 5/141 (3%)
Query: 5 QEQLTQMQHDLESGLDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFLSQKELNDNQ 64
+E+L Q + E + + + AN + V E N NLQ E L ++E+
Sbjct: 522 REELKQDKERFEKEWEALDKKRANITREQNEVAEENEKLRNLQIS-EKHRLKREEMTSRD 580
Query: 65 NKLSVDLEGLWQPIEVLRSTKADREEVADALRDKAGIAALNGLVSLQQFDAVQGDFEKRI 124
N L +L+G+ E S +AD E++ R+ + F+ +EKR
Sbjct: 581 N-LKRELDGVKMQKE---SFEADMEDLEMQKRNLDMEFQRQEEAGERDFNERARTYEKRS 636
Query: 125 LTAYDKFNNQEMIWQKAIDDL 145
D N + + Q+ ++++
Sbjct: 637 QEELDNINYTKKLAQREMEEM 657
>At1g15910.1 68414.m01908 XH/XS domain-containing protein / XS zinc
finger domain-containing protein contains Pfam domains
PF03469: XH domain, PF03468: XS domain and PF03470: XS
zinc finger domain
Length = 634
Score = 29.5 bits (63), Expect = 2.7
Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 1/63 (1%)
Query: 49 DLENMFLSQKELNDNQNKLSVDLEGLWQPIEVLRSTKADREEVADALRDKAGIAALNGLV 108
D E + KE+ND + +LEGL VL + + + A R K IA L GL+
Sbjct: 435 DDEAVQKKMKEMNDELDDKKAELEGLESMNSVLMTKERQSNDEIQAARKKL-IAGLTGLL 493
Query: 109 SLQ 111
+
Sbjct: 494 GAE 496
>At3g25960.1 68416.m03235 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 497
Score = 29.1 bits (62), Expect = 3.6
Identities = 24/95 (25%), Positives = 44/95 (46%), Gaps = 9/95 (9%)
Query: 16 ESGLDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFLSQKELNDNQNKLSVDLEGLW 75
+ G+ N + L N+ A +L ++ +E MFL+QK + + N +
Sbjct: 240 QEGVMNFDKILENSDAFMVARGDLG-----MEIPIEKMFLAQKTMINKANAHGKPVVTAT 294
Query: 76 QPIEVL----RSTKADREEVADALRDKAGIAALNG 106
Q +E + R T+A+ +VA+A+ D L+G
Sbjct: 295 QMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSG 329
>At1g05260.1 68414.m00532 peroxidase 3 (PER3) (P3) / rare
cold-inducible protein (RCI3A) (PRC) identical to
SP|O23044 Peroxidase 3 precursor (EC 1.11.1.7) (Atperox
P3) (Rare cold inducible protein) (RCI3A) (ATPRC)
{Arabidopsis thalliana}
Length = 326
Score = 28.7 bits (61), Expect = 4.7
Identities = 14/37 (37%), Positives = 21/37 (56%)
Query: 19 LDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFL 55
+ N AE LAN T+ +T L F+N DL+++ L
Sbjct: 150 ISNAAEALANIPPPTSNITNLQTLFANQGLDLKDLVL 186
>At5g43880.1 68418.m05366 expressed protein
Length = 836
Score = 28.3 bits (60), Expect = 6.3
Identities = 18/50 (36%), Positives = 23/50 (46%), Gaps = 3/50 (6%)
Query: 6 EQLTQMQHDLESGLDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFL 55
+ L H ES LD +L N+ D+T L + NL FDL N L
Sbjct: 678 DNLLSKWHSSESPLD---PSLRNSYADSTEQKRLGSNVKNLVFDLVNTLL 724
>At1g74290.1 68414.m08603 esterase/lipase/thioesterase family
protein contains Interpro entry IPR000379
esterase/lipase/thioesterase family
Length = 371
Score = 28.3 bits (60), Expect = 6.3
Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Query: 219 PALPAL--SSRSPGVGAESLNPKEDGDHLCYPRHPIPHQKDSRYII 262
P P L SS P + A + +DG +L Y H +P +K +R I+
Sbjct: 43 PPQPKLCGSSSGPPITAPRIK-LQDGRYLAYKEHGLPREKANRKIV 87
>At1g09470.1 68414.m01059 expressed protein ; expression supported
by MPSS
Length = 336
Score = 28.3 bits (60), Expect = 6.3
Identities = 26/103 (25%), Positives = 51/103 (49%), Gaps = 7/103 (6%)
Query: 36 VTELNGHFSNLQFDLENMFLSQKELNDNQNKLSVDLEGLWQPIEVLRSTKADREEVADAL 95
VT L NL+ +L SQK+L D K+ D + + + V++ TK + EV + L
Sbjct: 147 VTRLGEQLENLRKELRVRESSQKQLRDELLKVEGD---IMRAVSVVK-TK-ENSEVRNML 201
Query: 96 RDKA--GIAALNGLVSLQQFDAVQGDFEKRILTAYDKFNNQEM 136
+ +N L++ + + + E +I++A+ +F +E+
Sbjct: 202 NEDTPKNSERINKLLTAKDDEIARLRDELKIISAHWRFKTKEL 244
>At3g45850.1 68416.m04962 kinesin motor protein-related
kinesin-related protein TKRP125, Nicotiana tabacum,
PIR:T02017
Length = 1058
Score = 27.9 bits (59), Expect = 8.3
Identities = 37/182 (20%), Positives = 72/182 (39%), Gaps = 15/182 (8%)
Query: 3 NYQEQLT-QMQHDLESGLDNMAETLANAGGDTTAVTELNGHFSNLQFDLENMFLSQKELN 61
N Q+ LT ++ LE + ET + + N +F + N+ S+K L
Sbjct: 472 NSQQILTAELSEKLEKTEKKLEETEHSLFDLEEKYRQANATIKEKEFVISNLLKSEKSLV 531
Query: 62 DNQNKLSVDLEGLWQPIEVLRSTKADREEVADALRDKAGIAALNGLVSLQQFDA-VQGDF 120
+ +L +LE + L S ++++ D R +Q+F + +
Sbjct: 532 ERAFQLRTELESASSDVSNLFSKIERKDKIEDGNR-----------FLIQKFQSQLTQQL 580
Query: 121 EKRILTAYDKFNNQEMIWQKAIDDLFRGLNEKADYVQVTSLRDEIQKYLNMFDSRINAMS 180
E T QE+ + +D+ ++ K++ + LRD + K ++ S I A+
Sbjct: 581 ELLHKTVASSVTQQEVQLKHMEEDMESFVSTKSEATE--ELRDRLSKLKRVYGSGIEALD 638
Query: 181 EI 182
I
Sbjct: 639 NI 640
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.132 0.374
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,465,440
Number of Sequences: 28952
Number of extensions: 260321
Number of successful extensions: 809
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 805
Number of HSP's gapped (non-prelim): 15
length of query: 286
length of database: 12,070,560
effective HSP length: 80
effective length of query: 206
effective length of database: 9,754,400
effective search space: 2009406400
effective search space used: 2009406400
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 59 (27.9 bits)
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